Central Food Technological Research Institute
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Anti-aging potential of mealworm (Tenebrio molitor) protein-enriched fruit and vegetable-based products in in-vitro and in-vivo models
Nanoencapsulation of apocynin and vanillic acid extracted from Picrorhiza kurroa Royle ex Benth plant roots and its characterisation
Picrorhiza kurroa Royle ex Benth (P. kurroa) is an important medicinal plant in the ayurvedic system for treating various liver and inflammatory
conditions. The present study aimed to extract the phytocompounds from various extracts (Acetone, Chloroform, Ethanol, Ethyl acetate, Hexane,
and Methanol) of P. kurroa. Further, the major phytocompounds were nano-encapsulated by PLGA (Poly-lactic-co-glycolic acid) method and
characterized to enhance activity towards the target. The highest polyphenolic value was found to be 323.2 ± 16.6 and 316.3 ± 12.1 μg GAEq./mg
in ethanolic and methanolic extracts. The highest flavonoid value was found to be 280.3 ± 19.8 and 300.8 ± 15.2 in ethanolic and methanolic
extracts μg QEq./mg. P. kurroa exhibited DPPH radical scavenging with IC50 of 38.2 ± 1.1 and 43.7 ± 1.8 μg/mL and also showed potent ferric
reducing power and total antioxidant activities. The major phytocompounds, such as apocynin (AP) and vanillic acid (VA), were confirmed using
HPLC. Further, the nano-encapsulation of apocynin and vanillic acid successfully achieved by PLGA methods. The average particle size of nanoencapsulated
apocynin, vanillic acid is 350 nm, 204.4 nm, and zeta potential wer
Chitosan nanoparticles modulate plant growth, and yield, as well as thrips infestation in Capsicum spp.
The objective of this study was to investigate the physiological and biochemical effects of Chitosan nanoparticles
on Capsicum annuum plants. The particle size, polydispersity index, composition, and structure of the synthesized
chitosan-based nanoparticles (Chitosan (CS), Chitosan-Silver (CSAg), and Chitosan-Copper (CSCu) NPs) were
determined by analyzing the zeta potential, FTIR, TEM, and XRD. The seedlings showed improved physiological
and biochemical characteristics when 1, 10, and 20 ppm concentrations of nanoparticles (CS, CSAg, and CSCu)
were used for 24-h seed priming. The application of nanoparticles in different concentrations (0, 20, 40, 60, 80,
and 100 ppm) on the leaves of Capsicum spp. plants resulted in improved physiological traits and protection
against thrips by 70–85 %. Furthermore, it enhanced the content of chlorophyll (20–75 %), carotenoids (20–30
%), total phenolics (20–45 %), total flavonoids (40–125 %), reducing sugars (15–40 %), total antioxidant activity
(10–82 %), FRAP (10–100 %), DPPH (76-83 mg m