Central Food Technological Research Institute

Central Food Technological Research Institute, New Delhi: ePrints@CFTRI
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    18062 research outputs found

    Identification and quantification of vitamin A in fortified oils

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    Convective heat and moisture transfer coefficients during heat pump drying of Spirulina platensis extracts: effect of temperature and additives, and thermal analysis of powders

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    This work conducted heat pump drying of Spirulina platensis extracts and studied the effect of drying temperature and additives on drying characteristics, heat and moisture transfer parameters, and thermal properties of powdered extracts. A heat pump dryer with single-stage dehumidification and cooling was operated at 30 and 40 ◦ C, 10 % relative humidity, and 0.6 m/s air velocity. The cell-ruptured wet biomass extract was dried without additives (control, SE) and with 5 % w/w whey protein (SE + WP), dextrin (SE + DX), and maltodextrin (SE + MD). The experimental moisture ratio data agreed well with Dincer and Dost model under all drying conditions (R2, 0.9771 to 0.9933). The drying kinetics and moisture transfer parameters were significantly higher at 40 ◦C for all extracts. SE had the shortest drying time, highest moisture diffusivity, and maximum heat and moisture transfer coefficients at both temperatures, but the powder contained >12 % moisture content (wet basis). Whey protein was the most desirable additive, followed by dextrin. At 30 ◦C, SE + WP showed highest values of drying (5.047 × 10 4 s 1) and moisture transfer (3.498 × 10 7 m/s) coefficients. At 40 ◦C, SE + DX showed maximum moisture diffusivity (3.408 × 10 9 m2/s) and moisture transfer coefficient (4.953 × 10 7 m/s) but 6.15 % less heat transfer coefficient than SE + WP (6.044 ± 0.084 W/m2◦ C), p < 0.05. With whey protein, the melting temperature of SE was significantly increased, while SE + DX and SE + MD had water activity between 0.56 and 0.58 and the lowest glass transition temperature. The evaluation of heat and moisture transfer, drying kinetics, and powder properties facilitated the selection of suitable temperature and additive combination for cell- ruptured Spirulina platensis extract

    Protective effects of novel probiotic strains against LPS-induced inflammation in RAW264.7 macrophages

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    Plant-based fermented foods offer an underexplored reservoir of probiotic strains with therapeutic potential. In this study, we isolated and screened lactic acid bacteria (LAB) from spontaneously fermented vegetables through a comprehensive in-vitro approach involving stress toler- ance assays, antioxidant capacity, adhesion evaluations, and immune modulation studies using RAW 264.7 macrophages. Two standout strains, Lactiplantibacillus plantarum CRT01 (PP724070) and Levilactobacillus brevis CAB20 (PP499280), demonstrated strong resilience to gastrointesti- nal conditions, robust adhesion to Caco-2 cells, and significant free radical scavenging activity. Notably, both strains reduced reactive oxygen species and modulated LPS-induced inflammatory responses by downregulating TNF-α, IL-1β, IL-6, and IL-12, while enhancing IL-10 expres- sion. This dual antioxidant–immunomodulatory action, along with their ability to exclude pathogens, produce short-chain fatty acids, and exhibit low antibiotic resistance indices, highlights their suitability as non-dairy probiotics for gut-targeted interventions against oxidative stress and inflammation-associated disorders

    Precision biofumigation using plant monoterpenes and its molecular interaction with acetylcholinesterase to alleviate insect pests of stored sorghum

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    Improving the shelf life of sorghum during storage involves effective control of insect pests, which affect the grain quality and quantity. As an alternative to hazardous chemicals, ongoing research focuses on essential oils as a safer substitute. This study emphasizes on utilizing monoterpenes (α-Pinene, Eucalyptol, and p-Cymene) and their combinations to control three major storage insect pests: Sitophilus oryzae (L.), Rhyzopertha dominica (F.), and Oryzaephilus surinamensis (L.). The fumigant toxicity assay displayed complete mortality at the highest dose of 10 μL (Conc-333.3 μL/L air) for all tested compounds. The combination of α-Pinene and Eucalyptol confirmed potent fumigant toxicity against S. oryzae, R. dominica, and O. surinamensis, with LC50 values of 113.9 μL/L, 158.2 μL/L, and 80.1 μL/L respectively. The bulk biofumigation study showed that the α-Pinene and Eucalyptol combination exhibited the highest mortality rates (>95 %) for all insects within a 5-day cycle in Sorghum. Persistence analysis revealed reduced residue and no significant difference in seed viability of post-treated grains. Scanning electron microscopy analysis of treated insects showed significant damage in insect elytra. However, it’s noteworthy that these monoterpenes were not considerably effective against aflatoxigenic fungi. p-Cymene displayed remarkable AChE inhibitory activity towards S. oryzae (93.78 %), R. dominica (96.72 %), and O. surinamensis (81.52 %). Furthermore, computational studies were carried out to analyse the mode of in- teractions of selected monoterpenes against modelled AChE structures of S. oryzae and R. dominica. These results indicate the potential use of monoterpene and their combinations in formulating effective biofumigants to mitigate insect pests’ infestations in stored sorghum

    Characterization and application of zein-based composite films functionalized with PVA and peppermint extract in the preservation of harvested bell pepper

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    Capsicum annuum.L (bell pepper) is a very cultivated and economically important crop. However, the short shelf life of the fruit, due to its non-climacteric ripening nature and susceptibility to mechanical damage, leads to significant postharvest losses. Edible packing has emerged as a promising and sustainable solution to preserve quality attributes and extend shelf life during storage. In this study, we developed a composite film by blending zein (Z) and polyvinyl alcohol (P), with peppermint extract (M). The mixture of Z, P, and M (ZPM films) exhibited higher antioxidant (DPPH (42 %) and ABTS (52 %)) and antimicrobial activity compared to Z and P films, and the incorporation of peppermint extract improved UV light protection (22 %). FTIR, XRD, and SEM analyses confirmed the interaction between Z, polymer, and peppermint extract. Films were applied to preserve bell peppers and evaluated over 9 d at room temperature for their structural, physical, mechanical, and anti- oxidant properties. Bell peppers packed in ZPM films showed reduced weight loss (2.9 %), delayed softening, and better antioxidant activity compared to control fruits. The H₂O₂ content (5 mol kg 1) was significantly higher in control fruits than in those packed with ZPM films. Additionally, we examined the relative gene expression and specific activity of cell wall-degrading enzymes in bell peppers. Fruit packed in ZPM films exhibited lower enzyme activity and expression fold change than the control, suggesting delayed cell wall degradation. These findings demonstrate that ZPM films offer an effective and sustainable food preservation method, extending the shelf life of bell peppers while supporting the development of eco-friendly food packaging

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