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Elucidating the functional role of human ABHD16B lipase in regulating triacylglycerol mobilization and membrane lipid synthesis in Saccharomyces cerevisiae
No full textLipids are essential biological macromolecules that play a pivotal role in various physiological processes and
cellular homeostasis. ABHD16B, a member of the α/β-hydrolase domain (ABHD) superfamily protein, has
emerged as a potential key regulator in lipid metabolism. However, the precise role of human ABHD16B in lipid
metabolism remains unclear. In this study, we reported the overexpression of ABHD16B in Saccharomyces cerevisiae
to determine its physiological relevance in lipid metabolism. Through in vivo [14C]acetate labeling experiments,
we observed that overexpression of ABHD16B causes a decrease in cellular triacylglycerol (TAG)
levels and a concurrent increase in phospholipid synthesis in wild-type cells. Mass spectrometry (LC–MS/MS)
analysis further corroborated these findings, showing a significant decrease in TAGs with a carbon chain length
of 48 and an increase in major phospholipid species, specifically 34:2, upon overexpression of ABHD16B.
Confocal microscopy analysis revealed a reduction in the number of lipid droplets in strains overexpressing
ABHD16B, consistent with the observed decrease in neutral lipids. Additionally, qRT-PCR analysis indicated a
high phospholipid synthetic activity of ABHD16B and a potential decrease in TAG levels in wild-type yeast,
possibly due to upregulation of endogenous TAG hydrolytic enzymes, as confirmed using 3tglsΔ mutant strain.
Furthermore, GC-MS analysis revealed significant modifications in fatty acid composition upon ABHD16B
overexpression. Collectively, our results underscore the influence of ABHD16B overexpression on TAG levels,
phospholipid synthesis, lipid droplet dynamics, and fatty acid composition. These findings reveal a complex
interplay between TAG hydrolysis and phospholipid synthesis, highlighting the critical involvement of ABHD16B
in lipid homeostasis and providing further insights into its regulatory function in cellular lipid metabolism
Ginger and cinnamon infused sesame oil:unveiling health Benefits and flavor dynamics in a comprehensive research Thesis
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