Central Food Technological Research Institute
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Deamidation of wheat gluten using natural deep eutectic solvent (nades) and its impact on emulsion gel based fat replacers in bakery products
No full textOptimization of microwave assisted extraction of bioactives from capsicum annuum. (Byadagi) variety of Karnataka
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Quality evaluation in commercial whey powder: Detection and quantification of aflatoxin M1 and pesticide residues
No full textRole of monoacylglycerol lipase (MagL) in pearl millet (Pennisetum glaucum) seed germination
No full textAssessing genetic diversity of indigenous turmeric (Curcuma longa L.) through inter‑simple sequence repeat (ISSR) markers
No full textTurmeric (Curcuma longa L.), native to Southeast Asia, is renowned for its therapeutic properties, primarily due to its rhizomes containing various secondary metabolites, including the prominent compound curcumin. This study aimed to evaluate the genetic diversity among 35 turmeric genotypes from different geographical regions using Inter-Simple Sequence Repeat (ISSR) markers. Out of 116 amplified products, 110 (94.82%) were polymorphic, indicating significant genetic variation, while 6 (5.17%) products were monomorphic. The ISSR primer pairs generated between 8 to 11 bands each, averaging 9.67 bands per pair. Seven markers exhibited the highest polymorphism (100%), while UBC 850 showed the lowest (81.82%). Polymorphism Information Con-
tent (PIC) ranged from 0.18 to 0.46, averaging 0.33.
Resolving Power (RP) varied from 5.66 to 11.49, averaging 8.20. Effective Multiplex Ratio (EMR) values ranged from 8.44 to 25.45, with an average of 18.15. Marker index values ranged from 2.88 to 7.44, averaging 5.92, demonstrating the primers’ effectiveness in genetic diversity research. Unweighted pair group method with arithmetic mean (UPGMA) cluster analysis based on ISSR primers grouped the 35
turmeric genotypes into four main clusters. Cluster I,
sourced from Kerala, includes 15 accessions divided into four subclusters. Cluster II has three accessions: CIM-Pithambar and ACC Pratibha in one subcluster, and Suguna in another. Cluster III consists of Suvarna and Ladaw in one group, and Lasein and Lakadong in another, with three accessions from the northeastern region and one from Kerala. Cluster IV, the second-largest, includes 13 accessions from Karnataka, Kerala, Uttar Pradesh, Assam, and Maharashtra. These clusters highlight the genetic diversity and geographical distribution of turmeric accessions
Ultrasonication and ozone processing of Syzygium malaccense juice for retention of bioactive components
No full textFreshly extracted juice from two different varieties of an
under-utilized fruit (Syzygium malaccense) was subjected
to various non-thermal treatments, such as ultrasonication
(5 and 10 min at 50% amplitude with 30 s pulse lap time)
and ozonation (0.1 ppm for 20 min), along with the stan-
dard heat treatment (85 °C/10 min). Various treatment’s
effect on the microbiological, physicochemical, and bioac-
tive constituents of the juice were studied throughout stor-
age (28 days at 4 °C). Ultrasonication treatment enhanced
the bioactive components retention, and higher vitamin C,
TPC, DPPH, and ABTS radical scavenging activities were
observed in ultrasonicated (5 min) juices compared to oth-
ers. The ozone treatment showed deleterious effects on
wax apple juice, reducing TPC, vitamin C, and flavonoids.
Organic acid profiling revealed ascorbic, malic, fumaric,
and tartaric acids; major phenolic compounds included
catechin, rutin, syringic, vanillic, protocatechuic, gallic,
and rosmarinic acids in the fruit juice. Pearson correla-
tion coefficient highlighted positive correlations between
these compounds and antioxidant activity in both juices.
The present study addresses the scarcity of information on
wax apple juice constituents, highlighting its rich organic
acids and phenolics content. The results showed that ultra-
sonication (5 min) treatment emerged as an alternative to
thermal treatment, preserving bioactive compounds in wax
apple juice