Central Food Technological Research Institute

Central Food Technological Research Institute, New Delhi: ePrints@CFTRI
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    18062 research outputs found

    Optimization of matrix matched method for acrylamide analysis in chicory

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    Chicory is a coffee surrogate widely employed along with coffee in the beverage brewed from coffee-chicory mixture in India, few states of America, and France. In recent times, chicory has attracted attention in the context of higher acrylamide occurrence levels compared to coffee. The quantification studies have reported a significant variation in the amount of acrylamide present in roasted chicory samples. This considerable disparity in the reported acrylamide values prompted us to revisit the reported liquid chromatography tandem mass spectrometry (LC-MS/MS) method. The poor extraction and matrix interference were the significant contributors to the observed variations of acrylamide contents in roasted chicory. To address these issues, a matrix matched LC-MS/MS method is optimized for analysis of acrylamide content in roasted chicory matrix. The optimized LC- MS/MS method encompasses pre-spiking of roasted chicory sample with acrylamide and acrylamide-d3 followed by the generation of matrix matched calibration curve based on the MS response of pre-spiked samples. The extracted chicory samples were analysed using positive ion electrospray LC-MS/MS. The MS/MS data for the extracted chicory samples were compared with matrix matched calibration data to quantitate the acrylamide in roasted chicory samples. By employing developed matrix matched LC-MS/MS method, substantial number of chicory samples were screened for acrylamide content and the results are in the range of 152.81 ± 3.4–645.92 ± 0.55 μg/kg

    Effect of incorporation of nano encapsulated curcumin on the product quality of instant rice and extrudates from brown top millets: retention and stability studies

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    Curcumin, a bioactive compound known for its antioxidant and anti-inflammatory properties, has limited bioavailability due to poor solubility and rapid degradation during processing. With the growing demand for functional foods that are convenient and nutritionally enhanced, incorporating curcumin in staple-based value-added products can offer significant health benefits. The study aims to develop value-added products, quick-cooking rice (QCR) and millet extrudate, by incor- porating nano encapsulated curcumin (NEC). The quick-cooking rice was processed by steam cooking and fluidized drying followed by rehydration. Two formulations of NEC incorporated fluidized dried Bioactive quick cooking rice (BQCR-1 and BQCR-2) and Rehydrated Bioactive quick cooking rice (RBQCR-1 and RBQCR-2) were compared with their quick cooking rice control and rehydrated quick cooking rice control (QCR-C and RQCR-C) for their physico-chemical properties. The BQCR- 2 (6.6 mg/100 g) exhibited the highest curcumin retention. RBQCR-2 also showed the highest curcumin content with a concentration of 5 mg/ 100 g. Bio-accessibility testing revealed that sample BQCR-2 exhibited the highest release percentage. The subsequent objective was to explore bioactive-incorporated value-added products using brown top millet and to study the effect of extrusion on the retention of NEC. The flour was processed for brown top millet, maize, and rice, and three combinations were formulated with varying proportions. The flour with the initial NEC concentration showed the highest curcumin content (9.92/5.96 mg/100 g) before and after extrusion. The unique processing methods employed in this study offer a promising approach for enhancing the bioavailability and retention of curcumin in these products, which can have significant implications for their industrial applications in the future. Although the extrusion process led to some degradation of curcumin content, it still retained a substantial amount of the bioactive compound

    Can volatile sulphides act as fumigant or contact toxicant in cowpea weevil, Callosobruchus maculatus F.?: a comparative analysis

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    For understanding the toxic action of volatile sulphides, we have evaluated the insecticidal efficacy of sulphide mixture (diallyldisul- fide, diallylsulfide and diallyltrisulfide) against Callosobruchus macu- latus F. adults by fumigant toxicity (FT) and contact toxicity (CT) bioassays. Further, diffusional characteristics of sulphides inside the treatment vials were analysed by GC-FPD and microstructural impacts on treated beetles were observed using SEM. In the com- parative analysis, maximum level of insecticidal toxicity (0.88 µl/L of LC50), residue persistence (32.28 ppm/adult) and microstructural aberration in beetle elytra was observed in CT compared to FT bio- assay (1.60 µl/L of LC50 and 22.67 ppm/adult of residue). Whereas, the GC-FPD based diffusion analysis indicated even distribution of volatiles inside the vials in both FT and CT bioassays. The study results suggests that volatiles act as fumigant/vapours in both bio- assays with respect to even diffusion and toxicity occurs based on insect–volatile contacts inside the treatment chambers

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    Central Food Technological Research Institute, New Delhi: ePrints@CFTRI
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