Central Food Technological Research Institute
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A novel regulatory motif at the hinge dimer interface of the MksB mediates dimerization and DNA binding activity
No full textThe Structural Maintenance of Chromosome (SMC) protein is essential for various cellular processes, including chromosome organization, DNA repair, and genome stability. MksB, an alternative SMC protein present in prokaryotes, comprises a hinge dimerization domain and an ABC ATPase head domain linked by a coiled-coil arm. While hinge dimerization in bacterial and eukaryotic SMCs is attributed to
conserved glycines, our study unveils the critical role of a novel KDDR motif located at a loop near the hinge dimer interface in Mycobacterium smegmatis MksB (MsMksB). We demonstrate the regulatory role of this motif in MsMksB dimerization and DNA binding activity. The K600D mutation in the KDDR motif induces MsMksB dimer-to-monomer conversion, highlighting the significance of this motif in MsMksB
dimerization. Mass spectrometry-based mapping of the DNA binding site revealed the lysine's involvement in protein-DNA interaction. Monomers of the hinge domain lose DNA binding activity, and MsMksB single-arm mutants exhibit reduced DNA binding and ATPase activity, underscoring the importance of hinge-mediated dimerization in MsMksB function. Notably, the R603D mutant retains dimerization but
shows compromised ATPase and DNA binding activities. Mutants with defective ATPase activity exhibit impaired DNA condensation in vivo. These findings provide novel regulatory insight into the mechanism of MksB dimerization and DNA binding, uncovering the fundamental processes of chromosome
condensation and segregation
PEGylation as a tool to improve the stability of garlic sulfides as nano‑protectants from stored‑product beetles infestation
No full textThis study aimed to develop PEGylation based nanoparticles using garlic essential oil (GEO) and polyethylene glycol (PEG)
by melt-dispersion method. Physico-chemical characteristics of GEO loaded PEG nanoparticles (GPNs) was analysed by
Differential Scanning Calorimetry (DSC), Dynamic Light Scattering (DLS), and Gas Chromatography (GC) techniques.
Optimized GPN was subjected to stability and physico-chemical characteristics analysis up to six-month storage. Then,
insecticidal potentiality of GPN was investigated and microstructural impacts on the beetle surface was observed under
Scanning Electron Microscopy (SEM). Among the developed GPNs, DLS characteristics revealed GPN4 as optimal with
195.90 nm of particle size, 0.25 PDI, and 86.48% of encapsulation efficiency. Significantly, there was no physico-chemical
changes on GPN4 during six-month storage compared to control. In GC analysis, diallyl sulfide, diallyl disulfide, and dial-
lyl trisulfide were detected as major constituents of GEO and GPN4. The GPN4 showed potential contact toxicities against
Sitophilus oryzae, Rhyzopertha dominica, and Tribolium castaneum adults with 165.73, 169.95, and 192.82 ppm of LC 50
values at 72 h exposure. SEM observations exhibited adhesion of powder-form nanoparticles and abrasions on the cuticular
surface of beetles. In conclusion, the study results suggested that GPN4 was a stable and potential contact toxicant, which
could be useful as nano-protectant against stored-product beetles
Integrated Profiling of Aroma and Taste of various Tea Samples (Camellia sinensis) using E-Nose and E-Tongue
No full textUltrasound-Assisted Synthesis and Characterization of Tetrahydrocurcumin �-di-glucoside: A Strategy for Improved Solubility and Stability
No full textBioimprinting of lipases to enhance specificity towards medium chain fatty acids (MCFA's) in Coconut oil
No full textCymbopogon essential oils as biopesticides: Potential and challenges
No full textCymbopogon essential oils have proven effective as biopesticides used alone or alongside other agents against
diverse targets, including agricultural and stored grain pests, fungi, viruses, household pests, and blood-feeding
insects. They can be applied in multiple forms, such as fumigants, direct sprays, or granular formulations. Key
benefits include environmental safety, low impact on non-target species, fast-acting properties, and alignment
with sustainable farming practices. Additionally, they can be adapted to diverse environmental conditions.
Cymbopogon essential oils offer a promising alternative to chemical pesticides. Biotechnological advancements
can help scale up their use, and slow or extended-release formulations—such as gels, oil mixtures, or Nano
formulations—further enhance their potential. The industrial and economic benefits of Cymbopogon species for
small, medium, and large-scale farmers and entrepreneurs could significantly contribute to national growth. This
review summarizes current research on the application of Cymbopogon essential oils as biopesticides and ad-
dresses challenges associated with their use
Role of montmorillonite and other clays in the prebiotic reaction of D-ribose and amino acid
No full textRibose stability and availability is prerequisite for the prebiotic formation of RNA according to RNA world
hypothesis. The growing body of studies is suggesting the simultaneous evolution of RNA and protein. In
addition, RNA peptide world hypothesis is also receiving credible evidences. In view of the identification of these
prebiotic scenario, it is difficult to rule out interaction of ribose and amino acids in early Earth. In this paper, we
reveal the role of prebiotically plausible clay catalysts consisting of montmorillonite/bentonite/kaolinite in
prebiotic reaction of ribose with amino acids. The current study demonstrates that montmorillonite/bentonite/
kaolinite catalyze partial Amadori reaction of ribose and amino acids in solid state. On the other hand, in
aqueous state, montmorillonite/bentonite/kaolinite remain more or less as spectator and hardly any reactivity is
observed between ribose and amino acids. To get the insight on the role of montmorillonite/bentonite/kaolinite
in the reaction of ribose and amino acid in solid state and aqueous solution, a reactivity study was also conducted in methanol. The performed study uncovered the factors responsible for lability of ribose towards amino acids in
solid state and stability of ribose towards amino acids in aqueous solution
