Central Food Technological Research Institute
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Extraction optimization, partial purification, and characterization of Sialoglycoproteins from Piaractus brachypomus (Red Bellied Pacu)
2-Methyl tetrahydrofuran as an excellent alternative bio-based solvent for extractions of curcuminoids from turmeric (Curcuma longa)
Phytochemical analysis and insecticidal activity of Jatropha curcas extracts against Sitophilus oryzae
Metabolic and Biochemical Responses of Arthrospira Platensis (Spirulina) to Organic Nitrogen: An RSM-Guided Optimization Study
A study on the standardization of black-rice incorporated semi-dried noodles and evaluation of the bioactives during processing
A promising approach for improved citric acid production by Aspergillus niger with the assistance of cinnamaldehyde derivatives
Considering the importance of food preservation, this study presents the synthesis of cinnamaldehyde derivatives
i.e., chalcones and pyrazoles with an atom economy of >92 % using an energy-efficient and eco-friendly mi-
crowave-assisted synthesis with high purity and yield. All the compounds were evaluated for their ability to
enhance the growth of Aspergillus niger for citric acid production via submerged fermentation, which showed that
the pyrazoles significantly outperformed both untreated samples and chalcones in citric acid production, with
enhancements of up to 25-times and 4-times, respectively. Compounds at lower concentrations yielded citric acid
levels of 100–113 g/L. The purity and authenticity of citric acid recovered were confirmed through spectral
studies. Molecular docking against aconitase enzyme from nine different sources revealed various types of in-
teractions with binding affinities ranging from 7.2 to 10. The target compounds can be regarded as valuable
“lead compounds” for citric acid production, effectively addressing both demand and supply
Physicochemical characteristics of dietary fiber polysaccharides extracted from Murraya koenigii leaves and their functional role on gut homeostasis
Dietary fiber (DF) is an indigestible carbohydrate in plant foods that supports various physiological functions.
This study aimed to extract the soluble and insoluble dietary fiber (DF) from the curry leaves and investigate
their physicochemical properties as well as their functional role in the homeostasis of the gut microbiome. The study observed that insoluble-DF (IDF) yielded higher amounts than soluble-DF (SDF) across alkali, acid, and water extraction methods. Acid-extracted SDF showed the highest polysaccharide content (91.58 ± 1.53 %). Among IDFs, alkali-extracted IDF showed the highest polysaccharide content (81.93 ± 1.94 %). Glucose, arabinose, and xylose were identified as major monosaccharides. IDF had a larger particle size (463.5 ± 14.2 μm)compared to SDF (1.23 ± 15.55 μm), which influenced its physicochemical properties. IDF displayed better oil-holding capacity, while SDF showed superior water-holding capacity, potentially impacting glucose diffusion and cholesterol micelle formation. Furthermore, SDF and IDF promoted the growth of beneficial gut bacteria and increased production of short-chain fatty acids, which play critical roles in physiological regulation. Acid-extracted SDF restored gut homeostasis by increasing species richness and diversity, predominantly increasing beneficial bacteria and reducing pathogenic bacteria in LPS-induced dysbiotic mice. This study reveals the impact of extraction methods on the physicochemical and functional properties of curry leaves-DF, underscoring
its promise as a functional food for gut health
Development and characterization of carrageenan-based antibacterial films incorporated with natural melanin pigment from niger seed hulls (Guizotia abyssinica) and their efficacy to enhance the shelf-life of strawberries
Enhancing the safety and quality of minimally processed foods is a top priority for consumers and the food
industry. There is an urgent need for eco-friendly and sustainable packaging films to protect fresh food products.
In this study, we attempted to develop carrageenan-based films incorporated with melanin extract with enhanced
physicochemical properties, antibacterial activity, and effectiveness in maintaining the quality of strawberries
during storage. The carrageenan-melanin films were produced using a solution casting method, incorporating melanin extracted from niger seed hulls as a bioactive additive. The impact of melanin content on the structural, thermal, water barrier, antioxidant, and antimicrobial properties of the carrageenan-based films was assessed.
FTIR analysis validated the successful integration of melanin into the carrageenan matrix. The antibacterial
activity of the carrageenan-melanin (30 mg) films was tested against common foodborne pathogens, showing
effectiveness against Salmonella enterica (60 mm), Staphylococcus aureus (40 mm), Micrococcus luteus (36.67 mm), Klebsiella oxytoca (40 mm), Escherichia coli (42 mm), and Bacillus cereus (31 mm), outperforming the chloram-
phenicol control. The carrageenan-melanin film (30 mg) significantly restricted bacterial counts and effectively
extended the shelf life of strawberries stored at room temperature (25 ◦ C ± 2) for five days and maintained lower
heterotrophic bacterial count subsequently. Precise analysis of volatile organic compounds with a sophisticated
electronic nose (E-nose) system, revealed the freshness profile of strawberries during storage. Overall, the
carrageenan-melanin film shows great promise as a sustainable packaging material with enhanced antibacterial
properties for preserving strawberries, representing an innovative solution for sustainable food packaging