JOS - UNSOED (Jurnal Online Soedirman - Universitas Jenderal Soedirman)
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Kojic Acid from the Ethyl Acetate Fraction of Terminalia catappa Linn Fruit Flesh and its anti-cancer activity against HeLa cells
Full text linkABSTRACT. The ethyl acetate fraction of Terminalia catappa Linn. fruit flesh has anti-cancer activity against HeLa cells, with IC50 value is =165.37 ppm. The aim of this research is to isolate active anticancer compound from the ethyl acetate fraction of T. catappa fruit flesh. Anti-cancer active compounds from the ethyl acetate fraction of T. catappa fruit flesh were purified using column chromatography and continued with recrystallization. The compound isolate from the ethyl acetate fraction is clear yellowish crystals and has a melting point 150 ± 2 oC, has anti-cancer activity against HeLa cells of IC50 =413.695 ppm. Results of molecular structure identification using UV spectroscopy; FTIR; 1HNMR, 13CNMR and LC-MS are thought to be the compound 5-Hydroxy-2-(hydroxymethyl)-4H-pyran-4- one (Kojic acid) with a molecular weight of 142.36 gram/mol. The Kojic acid content in T. catappa Linn fruit flesh was found to provide information on the potential use of T. catappa fruit flesh extract in the pharmaceutical and cosmetics industries.
Keywords: Anticancer activity, HeLa cells, Kojic acid, T. catappa Linn
REPRESENTATION OF STUDENT BULLYING ACTS IN KOREAN DRAMA (ROLAND BARTHES SEMIOTIC ANALYSIS IN THE GLORY DRAMA SEASON 1)
Full text linkThis article presents the results of research on bullying acts in the Korean drama series The Glory Season 1 (2022). The drama depicts acts of revenge by victims of bullying during their teenage years. This research is qualitative and utilizes Roland Barthes’s semiotics method for analysis. The study results demonstrate that the acts of bullying perpetrated by individuals can be categorized into several types. Bullies will continue to harass victims until they are satisfied. Through this research it was also found that films can be an examples for the audience to take certain actions
Geologi Dan Zonasi Kerentanan Gerakan Tanah Menggunakan Analytical Hierarchy Process Daerah Kebanaran Dan Sekitarnya, Kecamatan Mandiraja, Kabupaten Banjarnegara, Jawa Tengah
Full text linkDaerah Kebanaran dan Sekitarnya, Kecamatan Mandiraja, Kabupaten Banjarnegara, Jawa Tengah merupakan daerah yang berlokasi di tepi Sungai Serayu dan berbatasan dengan jalur pegunungan selatan. Daerah penelitian juga merupakan daerah yang memungkinkan terjadinya gerakan tanah, hal ini diakibatkan adanya beberapa faktor alamiah dan faktor aktivitas manusia yang menyebabkan gerakan tanah. Dilakukannya penelitian ini yaitu untuk menjelaskan hubungan kondisi geologis terhadap faktor kerentanan gerakan tanah di daerah penelitian. Metode yang digunakan adalah metode Analytical Hierarchy Process (AHP). Metode ini mencocokkan beberapa parameter yang dianggap sebagai faktor terjadinya gerakan tanah dan diberikan nilai berdasarkan kriteria pembobotan. Berdasarkan hasil penelitian, terdapat beberapa parameter, seperti faktor alam, geologi, dan aktivitas lainnya yang mempengaruhi gerakan tanah di daerah penelitian yang diantaranya adalah intensitas curah hujan, litologi, kerapatan sungai, strukur geologi, tata guna lahan, serta kemiringan lereng. Parameter – parameter tersebut kemudian diberikan penilaian dan pembobotan sebagai untuk mendapatkan nilai akhir dalam menentukan zona kerentanan gerakan tanah. Hasil dari perhitungan tersebut menghasilkan 4 tingkat gerakan tanah yaitu zona kerentanan gerakan tanah rendah, zona kerentanan gerakan tanah sedang, zona kerentanan gerakan tanah tinggi, dan zona kerentanan gerakan tanah sangat tinggi
Deliberative Policy in The Cooperation Process For The Development of Joint Village Owned Enterprises (Bumades) in Banyumas Regency
Full text linkThis research aims to determine the deliberative policy process for cooperation in the development of joint village-owned enterprises (BUMADes) in Banyumas Regency. The role and institutional objectives of BUMADes are efforts to optimize the development of village resources and potential as a whole. The BUMADes development policy is an alternative in optimizing the management of the potential possessed by each village, so that it can be managed jointly to support and improve the economy of the village community. This research method uses descriptive qualitative research with a case study approach to the management of joint village-owned enterprises. The focus of the research is a deliberative approach in the BUMADes development process in Banyumas Regency, which is based on aspects of Representation and Recruitment, Framing and Forum Design, Facilitating Conversations and Synthesis of Results. The research results show that the deliberative process is an effective model in implementing cooperation between villages, especially implementing joint BUMADes through a system of communication flow, commitment and providing mutual benefits. In terms of management, BUMADes together is able to optimize village economic development in a sustainable manner. The policy for managing jointly owned business entities is one of the strategic steps in developing joint village-owned business entities and is a step in building partnerships between village. The policy for developing joint village-owned enterprises is an effort to support progress in developing village potential in a synergistic and participatory manner
Computational Study on Apoptosis-Inducing Factor (AIF)-Mediated Modulation of Menadione using Molecular Docking and Parametrized Quantum Methods
No full textABSTRACT. Apoptosis-inducing factor (AIF) is a protein that is crucial for apoptosis which its dysregulation has been connected to the emergence of cancer. Mitochondria are organelles that in healthy cells function as energy factories that are important for maintaining cell life. AIF is located in the mitochondrial intermembrane space with active sites, namely FAD and NADH. Meanwhile in the recent studies, quinone compounds have shown potential as anti-cancer agents by targeting mitochondrial function, but the mechanism is still unclear. In this study, we used computational methods, including molecular docking and the Divide-and-Conquer Density Functional Tight-Binding Molecular Dynamics (DCDFTBMD) method, a type of parametrized quantum calculation, to investigate the interaction between mitochondrial AIF and menadione, as a quinone compound with anticancer potential. From the calculation result, AIF interaction with menadione was stronger in the FAD domain than in NADH. The partial charges of the ligands before and after the interactions were calculated using the DCDFTBMD technique, and the results show that the charge in the bonding area becomes more negative. This indicates the strengthening of electrostatic interactions through polarization effects in the bonding molecule. Additionally, it was discovered through this study's data that all ligands interacted with the ser-175 residue in the AIF protein. These residues are modulated by the involvement of AIF in the cell death process. From this study, it can be concluded that menadione has the potential as an anticancer agent through the AIF modulation mechanism.
Keywords: AIF, enzyme-catalyzed modulation, docking, quantum parametrization, menadion
Synthesis and Cytotoxic Activity of Methoxylated Chalcones in Breast Cancer MCF-7 and Prostate Cancer DU-145 Cell Lines
No full textChalcones, a class of naturally occurring compounds, exhibit a broad spectrum of biological activities, including anticancer properties. In this study, a series of methoxylated chalcones were synthesized via Claisen-Schmidt condensation and evaluated for cytotoxic activity against breast cancer MCF-7 and prostate cancer DU-145 cell lines. The synthetic route involved Claisen-Schmidt condensation, leading to various methoxy-substituted chalcone derivatives. The structures of the synthesized chalcones were confirmed through NMR and mass spectrometry. Cytotoxicity was assessed using the PrestoBlue assay, with 4-bromochalcone (compound 2) displaying the highest cytotoxic activity against MCF-7 cancer cell lines (IC50 = 26.99 µM). These results indicate that methoxylated chalcones hold promise as potential lead compounds for the development of new anticancer agents targeting breast and prostate cancer
Development and Validation of Zerumbone Analysis Method Using Ultraviolet-Visible Spectrophotometry
No full textABSTRACT. Analytical method development ensures that an analytical procedure is suitable for the identification and quantification of compounds of interest. This study aimed to develop and validate a simple, cost-effective analytical method for the determination of zerumbone using UV-Vis spectrophotometry. Method validation was conducted according to ICH Q2(R2) guidelines, including tests for specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, and precision. Zerumbone exhibited a maximum absorption wavelength (λmax) of 212 nm when dissolved in dimethyl sulfoxide (DMSO). The method demonstrated excellent linearity with a determination coefficient (R2) of 0.9991 across a concentration range of 10-50 μg/mL. Accuracy, evaluated via recovery studies, ranged from 91,00% to 110,45%. Precision testing results in a % RSD of 0.58%. The calculated LOD and LOQ were 1.55 μg/mL and 4.70 μg/mL, respectively. This validated method is simple, rapid, and economical, making it a promising alternative for zerumbone analysis in quality control laboratories.
Keywords: Zerumbone, Method Development, UV-Vis Spectrophotometry, Validation, ICH Guidelines.
 
Translation Problems and Techniques of Key Legal–Commercial Terms in Abbott’s Purchase Order
No full textThis study analyzes the translation problems and translation quality of key legal–commercial terms in Abbott’s Purchase Order Terms and Conditions, a bilingual corporate document used in international business. Adopting a qualitative descriptive approach, 60 legally significant terms were examined using Spradley’s componential analysis to identify problem types, Molina and Albir’s (2002) taxonomy to classify translation techniques, and Nababan et al.’s (2012) Translation Quality Assessment model to evaluate accuracy, acceptability, and readability, supported by expert validation. The results showed that major translation problems include system-bound non-equivalence, ambiguity, under-translation, modality errors, and terminology inconsistency, largely caused by conceptual gaps between English common-law terminology and Indonesian civil-law concepts. Calque emerged as the most frequently used technique, often producing unnatural, unclear, or misleading renderings, while established equivalents resulted in more accurate and acceptable translations. Literal and calque frequently weakened legal precision by reproducing surface linguistic forms without conveying the functional legal effects of the source text. Overall, the study concludes that translating legal–commercial terminology requires linguistic competence, comparative legal knowledge, and consistent terminology management. The findings highlight the need for improved translation practices, more rigorous post-editing of machine-assisted output, and closer collaboration between translators and legal experts to ensure clarity, accuracy, and enforceability in bilingual corporate documents
Electronic Properties of Fe3-xCoxO4 (x = 0.25 and 0.375) Synthesized from Natural Iron Sand: Evaluation of Band Gap and Urbach Tail
No full textFe3-xCoxO4 nanoparticles (x = 0.25 and 0.375) were successfully synthesized using natural iron sand as the base material through a coprecipitation method with the addition of CoCl2·6H2O as a cobalt source. Optical characterization was carried out using UV–Vis spectroscopy in the wavelength range of 200–800 nm to analyze the electronic properties of the material. The measurement results showed that increasing the cobalt fraction strengthened the absorption in the visible region and shifted the absorption onset to lower energies, indicating a narrowing of the band gap energy. Tauc analysis revealed that the direct gap energy decreased from 2.271 eV (x = 0.25) to 2.221 eV (x = 0.375), while the indirect gap energy decreased from 2.222 eV to 2.204 eV. Furthermore, the Urbach energy calculation shows an increase from 0.073 eV at x = 0.25 to 0.074 eV at x = 0.375, indicating an increase in local disorder that broadens the band tail. This finding is consistent with previous research reports on Co-doped magnetite and CoFe2O4, and confirms that cobalt doping is effective in enhancing the visible light absorption capacity. Thus, Fe3-xCoxO4 based on natural iron sand, especially at x = 0.375, has potential applications as an active material in visible light-based photocatalysis and solar energy optoelectronic devices.Fe3-xCoxO4 nanoparticles (x = 0.25 and 0.375) were successfully synthesized using natural iron sand as the base material through a coprecipitation method with the addition of CoCl2·6H2O as a cobalt source. Optical characterization was carried out using UV–Vis spectroscopy in the wavelength range of 200–800 nm to analyze the electronic properties of the material. The measurement results showed that increasing the cobalt fraction strengthened the absorption in the visible region and shifted the absorption onset to lower energies, indicating a narrowing of the band gap energy. Tauc analysis revealed that the direct gap energy decreased from 2.271 eV (x = 0.25) to 2.221 eV (x = 0.375), while the indirect gap energy decreased from 2.222 eV to 2.204 eV. Furthermore, the Urbach energy calculation shows an increase from 0.073 eV at x = 0.25 to 0.074 eV at x = 0.375, indicating an increase in local disorder that broadens the band tail. This finding is consistent with previous research reports on Co-doped magnetite and CoFe2O4, and confirms that cobalt doping is effective in enhancing the visible light absorption capacity. Thus, Fe3-xCoxO4 based on natural iron sand, especially at x = 0.375, has potential applications as an active material in visible light-based photocatalysis and solar energy optoelectronic devices
Penggunaan Fitur Close Friends Pada Second Account Instagram Sebagai Self Disclosure (Studi Dramaturgi Di Kalangan Mahasiswa Ilmu Komunikasi Universitas Nusa Cendana)
No full textThis research aims to find out the meaning and use of the use of the close friends feature on the second Instagram account of Nusa Cendana University Communication Science students and to see the display of self-disclosure carried out through the close friends second account feature and also to describe how messages are managed on the front stage and back stage in the close friends second account Instagram feature of Nusa Cendana University Communication Science students on the first and second Instagram accounts. This type of research is a type of qualitative descriptive research where the researcher carries out observations, interviews and documentation with several informants. The theory used is Erving Goffman's Dramaturgical Theory. The results of this research show that Nusa Cendana University Communication Science students interpret and utilize, display self-disclosure and manage messages using the close friends second account feature on Instagram because they want to separate their formal personal life from their daily activities which are more flexible, they want to brand themselves well on their first account while being more self-disclosure or open self-disclosure on the close friends second account feature on Instagram by separating only trusted people who can see their Instagram stories. With that, posting random things, private things, togetherness with family and friends, romantic relationships and their work is included in the back stage of Dramaturgical theory, while posting formal things such as birthday greetings, aesthetic photos of oneself is included in the front stage of Dramaturgical theory