Advanced Emergency Medicine (E-Journal)
Not a member yet
458 research outputs found
Sort by
Prediction of Overall Survival in Gastric Cancer by a Six-Gene Cox Proportional Hazards Model
Get PDFThis study aims to investigate prognostic genes that are correlated with overall survival in gastric cancer. A list of 43 critical genes is first selected from literature review and is narrowed down using marginal analysis, false discovery rate (FDR). This study used the 16 differential genes selected by FDR to create a cox proportional hazards regression model. Using a stepwise approach, the cox model is refined. From the 16 genes, 6 genes significantly correlated with overall survival are chosen and kept in the cox model. A principal component regression model was also constructed based on the principal component analysis results. The concordance of the principal component regression model is then compared to the concordance of the cox proportional hazards regression model. The final 6 identified genes are NRP1, STK11, MCM2, MARCKS, CTS6, C5. Of the 6 genes, MARCKS, NRP1, STK11, MCM2 are in line with previous research. CTS6 and C5, although studied in other cancers, are comparatively novel in the field of gastric cancer
Application Effect of “3P†Nursing Management Mode in Emergency Patients with Acute Ischemic Stroke
Get PDFObjective:To explore the application effects of the 3P(perfecting equipment,perfecting nursing ability, perfecting emergency pathway and application) nursing management mode of acute ischemic stroke nursing care. Methods: Clinical data of 80 cases of patients were selected as research group,and 80 acute ischemic stroke patients were selected as reference group. Results: Vein opening time, CT completion time and thrombolysis DNT in the research group were significantly lower than that of the reference group（P < 0.05. Conclusion: The "3P" management mode can significantly shorten the patients' vein opening time, CT completion time and DNT time of thrombolysis, improve the first aid effect,improve the treatment effect of AIS, which can be widely used
Meta-Analysis of Chinese Patent Medicine Combined with Zoledronic Acid in the Treatment of Primary Osteoporosis
Get PDFObjective: To systematically evaluate the safety and efficacy of Chinese patent medicine combined with zoledronic acid in the treatment of primary osteoporosis. Methods: CnKI, VIP, Wanfang Database, Chinese Biomedical literature database, PubMed, Web of Science, The Cochrane Library, Embase and CnKI were searched by computer RCTs of clinical randomized controlled trials of Chinese patent medicine combined with zoledronic acid in the treatment of primary osteoporosis in Embase database were retrieved from the database construction to April 1, 2022, and meta-analysis was performed using RevMan 5. 3 software. Results: A total of 14 RCTs were included, and the results of meta-analysis showed that:Compared with the control group, Chinese patent medicine combined with zoledronic acid had significant effects on the improvement of total clinical response rate, VAS score, MDL score, lumbar vertebra bone mineral density, femoral neck bone mineral density, Ward's triangle bone mineral density, osteocalcin and ALP, and significantly reduced alkaline phosphatase, with statistically significant differences. It had no significant effect on serum phosphorus, calcium and adverse reactions (P >0. 05). Conclusion: The safety and efficacy of Chinese patent medicine combined with zoledronic acid in the treatment of POP are clear, but the quality of the included studies is limited, and more high-quality studies are needed to enhance the evaluation evidence
Trimethylamine-N-Oxide: Its Role in Cardiovascular Disease, Mechanism and Potential Therapeutic Targets
Get PDFCardiovascular disease (CVD) is a common circulatory system disease and the collective name of all kinds of heart diseases, such as rheumatic heart disease, congenital heart disease, hypertensive heart disease and so on. According to WHO’s publishment, CVD is the top three leading cause of death in the world. Therefore, it is necessary to study the risk factors of CVD. In recent years, there has been increasing research on the effect of diet on CVD, and red meat caught the attention of researchers. L-carnitine in red meat can eventually be oxidized to trimethylamine-n-oxide (TMAO) by the gut flora. In this review, I give evidence-based conclusions about the relationship between L-carnitine and CVD, metabolism of TMAO in the presence of intestinal microorganisms. I also discuss the mechanism by which TMAO increases the CVD risk and possible therapeutic targets offered by TMAO. Overall speaking, it is a promising research direction to find therapeutic targets by blocking L-carnitine’s formation
Computed Web Learning Software Design with a Medical Psychological Perspective: Depression as an Example and Economic Analysis
Get PDFWe have tried to use computer technology in teaching and designing the necessary knowledge points for the diagnosis, treatment, and prevention of depression. We have also used computer platforms to elucidate this model as an economics product and carry out the necessary investigation and study of the market prospects, and we have proposed innovative points in solving the problem based on basic knowledge in medical psychology, and we have reported the results in conjunction with the results of the study
Effect of NE Infusion on Postoperative Delirium in Elderly Patients Undergoing PFNA Internal Fixation
Get PDFObjective: To observe the effect of blood pressure management by norepinephrine infusion on postoperative delirium (POD) in elderly patients undergoing PFNA internal fixation. Methods: 80 elderly patients undergoing elective PFNA internal fixation under spinal anesthesia, aged >65 years, ASA II or III, with body mass index(BMI) of 16.9 kg/m2 to 27.8 kg/m2, were included and divided into a test group(group T) and a control group (group C) using the random number table method, with 40 cases in each group. In group T, patients received an infusion of 8μg/mL norepinephrine (NE) that was begun at 15 mL/h immediately after spinal anesthesia, and then adjusted within the range 0-30mL/h according to systolic blood pressure(SBP) values measured noninvasively at 2-minute interval until the patients left the operating room, with the goal of maintaining values from baseline to 20% above the baseline; in group C, SBP values was allowed to fluctuate ±20% baseline by giving a bolus of 8μg NE(8 µg/mL). The incidence of POD within 7 days after surgery was recorded. SBP values, SpO2, and HR were recorded at the following endpoints: before the induction of anesthesia (T1),4 minutes after anesthesia (T2), before incision (T3), 4 minutes, 10 minutes, 20 minutes after incision (T4-6), at the end of surgery (T7), 5 minutes before leaving the room (T8).NE amount and occurrence of adverse events were recorded. Results: Compared with group C, the incidence of POD was reduced, SBP values at T2 to T8 and NE amount was increased (P<0.05). Conclusion: Infusion of 8µg/mL of NE maintaining SBP values from baseline to 20% above the baseline reduces the incidence of POD in elderly patients undergoing PFNA internal fixation
A Study on the Effect of IL-17A on Phenotypic Transformation of Fibroblasts in Bleomycin-Induced Pulmonary Fibrosis in Mice and Its Mechanism
Get PDFObjective: In this study, lung fibroblasts were cultured and identified in mice lung fiber model with bleomycin. Under the induction of IL-17A, lung fibroblasts were gradually transformed into myofibroblasts in pulmonary fibrosis, and the specific induction effect of IL-17A in pulmonary fibrosis was analyzed, which could provide ideas for the prevention and treatment of clinical pulmonary fibrosis. Methods: To investigate the transcriptional expression of bleomycin-induced fractional pulmonary fibrosis in different pulmonary fibrosis processes. The 14-day mice model was taken as the research object, and the pulmonary fibrosis model was established by induction of myogenesis. After 14 days of modeling, lung tissue was removed, and after centrifugation and repeated adherent treatment, lung fibroblasts could be cultured at the origin. After three generations of culture, the morphological changes of lung fibroblasts could be observed under a microscope. Indirect immunofluorescence was used to establish the expression of vimentin, and IL-17 was used to stimulate primary cultured lung fibroblasts to detect the expression and specific localization of a-SMA in cells. Western blotting was used to stimulate the expression of lung fibroblast protein by IL-17A at different time points. Results: The typical characteristics of primary culture lung fibroblasts were obtained. After purification and culture, lung fibroblasts were obtained in morphology. The morphology of the 3rd and 4th generation cells was relatively uniform, showing long carboxyform. 1-2 nucleoli can be observed by microscope, which have distinct cell boundary and are lined up like fish schools. The results of indirect immunofluorescence showed that the vimentin staining in the third generation cells was positive, and the plasma was dark red. There were collagenous fibrous septa between the cells, which might make them develop into lung fibroblasts. A-SMA immunofluorescence results showed that in the absence of IL-17A induction, A-SMA signal was relatively weak in the lung fibroblasts of the control group and was in the cytoplasm, while after IL-17A induction, A-SMA signal was stronger in the lung fibroblasts of mice and the whole cells presented spindle structure. Western bletting showed that lung fibroblasts were stimulated by IL-17 in the 0h group. Compared with the 1h, 2h, and 4h groups, the expression of A-SMA in lung fibroblasts was significantly increased in the 1h, 2h, and 4h groups. The fibroblasts were very low in the 2h and 4h groups. There was no significant difference in the expression of AS MA signal. Compared with 0h, protein contents of p-IKB-a and p-p65 were higher in lung fibroblasts at 1h, 2h and 4h. Protein expressions of Acti, 1P6, IKB-a and P65 were different in lung fibroblasts, but there was no significant difference. However, there was no significant statistical difference in the expression of these proteins in lung fibroblasts at different times. Conclusion: By differential centrifugation and repeated adhesion, bleomycin-induced lung fibroblasts can be isolated and purified, and more cell production can be obtained. The staining vimentin was strongly positive after identification by indirect immunofluorescence. The stimulation of IL-17A could gradually transform non-fibroblasts into myofibroblasts and play an important role in pulmonary fibrosis. Therefore, through experimental studies, it was found that IL-17A stimulated F-kB signal and then increased the expression of P-IKB-a and P-P65 proteins, and transformed non-phosphorylated proteins into phosphorylated proteins, thus transforming lung fibroblasts into myofibroblasts and playing a role in pulmonary fibrosis
Study on the Bacteriostatic Effect of Baitouweng on Pseudomonas Aeruginosa Infection of Wounds in Rats
Get PDFObjective: To analyze the bacteriostatic effect of Baitouweng on Pseudomonas aeruginosa infection of wounds in rats. Methods: Forty Wistar rats were enrolled in the study, among which excisions were made on 30 rats on their upper layer of dorsal skin with an area of 1 cm x 1 cm, the other 10 rats as the control group of sterile wound. Prepared Pseudomonas aeruginosa was applied on the wounds of rats to create infection models. Forty rats were divided into three groups (control group, mafenide group and Baitouweng group) according to different infection methods, and were treated with normal saline, 100g/L mafenide, and 1g/L Baitouweng respectively after 3 hours of injury. The changes in the number of white blood cells in both the wound surface and body of the three groups were observed within one to four days after injury. After that, the changes on the number of both white blood cells and body weight were continuously observed. The survival of the rats in each group was observed on the 14th day after injury. Results: From the observation after injury, compared with the other two groups, rats in control group had more exudation and moist wounds, and the activities of rats decreased while the death rate increased. On the 3rd day after injury, the number of white blood cells in each group decreased, and the number of Pseudomonas aeruginosa in the control group was significantly higher than that in the other two groups (P < 0.01). The rats in the sterile wound control group did not die and continued to gain weight. After 14 days, the survival number of rats in control group was significantly less than that in mafenide group and Baitouweng group (P<0.05). Conclusion: Baitouweng has obvious bacteriostatic and virus-killing effects on Pseudomonas aeruginosa infection of wounds in Wistar rats, reducing mortality rate effectively, and has practical value as well as development and application prospects
Effects of Perioperative Psychological Intervention on Rehabilitation Process of Patients with Total Knee Arthroplasty
Get PDFBackground: This study focuses on evaluating the effects of perioperative psychological intervention on rehabilitation process of patients with total knee arthroplasty (TKA). Method: We selected 40 patients randomly which all need to receive total knee arthroplasty in Nanjing Drum Tower Hospital during the period from January 2022 to March 2022. The patients were randomly assigned to two Groups (20 in each group): an intervention group (Psychological intervention combined with routine nursing, drug and rehabilitation therapy) and a control group (routine nursing, drug rehabilitation therapy). During each patients’ perioperative TKA surgeries, three scales (including VAS, ROM and ADL) are used to assess two groups. Result: After one week of psychological intervention, the pain score of the intervention group was lower than that of control group, the knee motion was greater than that of control group, and the ADL score was higher than that of control group. There was a significant difference in the treatment recovery between the two groups (P<0.05) Conclusions: Perioperative psychological intervention can promote the rehabilitation process of TKA patients, It can significantly improve pain, joint activity limitation, disuse muscle atrophy and other problems in a short period of time after surgery. Besides, it will effectively help patients to overcome the fear of movement, anxiety and improve patients' confidence, rehabilitation cooperation and prevention of complications, make patients adapt to the later rehabilitation life
Research Progress of Each Cell Signaling Pathway in Renal Interstitial Fibrosis and Anti-Fibrotic Intervention Countermeasures
Get PDFInterstitial fibrosis is a common pathological feature of various progressive renal diseases, andthis result is mainly caused with the activation of renal interstitial innate cells (fibroblasts, pericytes, immune cells, mesenchymal stem cells, etc.) and the massive expression and deposition of extracellular matrix（ECM）. According to statistics,chronic kidney disease and interstitial renal fibrosis affect half of the world's adults over the age of 70 and 10% of the population.Although there are currently no drugs or other means to halt this process, as more and more key players affecting fibrosis are identified, this provides new research directions for anti-fibrotic therapy. In this review, we highlight the relationship between renal interstitial lamina propria and the progression of interstitial fibrosis and describe new advances in anti-fibrotic strategies.Finally,we hope to provide new ideas for the treatment of interstitial renal fibrosis