Asian Journal of Pharmaceutical Research and Health Care (AJPRHC)
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STRUCTURE BASED DRUG DESIGN STUDIES ON HETEROARYL PROPANOIC ACID DERIVATIVES AS PPARΓ AGONISTS
Full text linkPeroxisome proliferator-activated receptors (PPARs) are a group of nuclear receptorproteins. Docking studies are based on several factors. Among 15 entries of PPARγ, 2Q6S wastaken for docking analysis, as it showed 418 most favored regions, 35 in additionally allowedregion and none of the residue in disallowed regions. To carry out drug designing, moleculeswere considered from the literature in which substitution of R1 position with dihydrofurylreported to have high dock score (-14.98 Kcal/mol) than the remaining analogues, with bettergeometry and interactions. Hence docking analysis using heteroaryl propionic acid derivatives asanti-diabetic agents suggest the reproducibility of active molecules being predicted bycomputational docking studies using Auto dock software
IMMUNOMODULATORY ACTIVITY OF BALANITE ROXBURGHI
Full text linkBesides having traditional use as animmunomodulatory action, it is not well scientificallyestablish. Hence present study was undertaken to screenthe immunomodulatory activity of ethanolic extract ofBalanite roxburghi leaves.Traditionally, leaves of Balaniteroxburghi are claimed to possess immunomodulatoryactivity and hence the reason behind evaluating theimmunomodulatory activity of ethanolic extract ofBalanite roxburghi (EBR) leaves. The animals weredistributed into three groups consisting of six animalseach. The first group served as control, the second andthird group received low dose and high dose of ethanolicextract of Balanite roxburghi (EBR) at 200 mg/kg, p.o.and 400 mg/kg, p.o. respectively. Theimmunomodulatory activity of EBR was evaluated inCarbon clearance test, Effect on serum immunoglobulin,Cyclophosphamide induced neutropenia. In all theseparadigms 400mg/kg dose of EBR was more effectivethan its 200 mg/kg of dose. These results suggest thatBalanite roxburghi can be use as immunomodulator
ESTIMATION OF GATIFLOXACIN IN PHARMACEUTICAL DOSAGE FORMS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Full text linkA simple, rapid, sensitive and preciseHigh Performance Liquid Chromatographic(HPLC) method has been developed for theestimation of gatifloxacin in its pharmaceuticaldosage forms. In this method RP-C18 column(150mmx4.6mm I.D., 5 m particle size) withmobile phase consisting of acetonitrile and 0.05Mphosphate buffer in the ratio of 25:75 v/v inisocratic mode was used. The detection wavelengthis 293nm and the flow rate is 0.8ml/min.Ciprofloxacin is used as internal standard. Thelinearity was found to be in the range of 0.1 to 10g/ml and shows a correlation coefficient of0.9999. The intra- and inter-day variation wasfound to be less than 1.2% showing high precisionof the assay method. The mean percent recovery ofthe drug from the solution containing 4 g/ml was100.5 2.06 indicating high accuracy of theproposed method. Hence, this method is simple,fast, specific, accurate, precise and less timeconsuming for the estimation of gatifloxacin inpharmaceutical dosage form
POLYCYSTIC OVARY SYNDROME
Full text linkPolycystic ovary syndrome is a condition in which a woman has an imbalance of female sex hormones. This may lead to menstrual cycle changes, cysts in the ovaries, trouble getting pregnant, and other health changes. In PCOS, mature eggs are not released from the ovaries. Instead, they can form very small cysts in the ovary. These changes can contribute to infertility. Common symptoms of PCOS include Menstrual disorders, Infertility, High levels of testosterone and Metabolic syndrome. Obesity, sedentary life style with inadequate physical activity, stress, junk food consumption are thought to be contributing factors in addition to genetic origin. In recent years many of the girls and women are suffering from PCOS because of wrong eating habits, stressful living conditions and lack of physical activity. Weight loss has been the major recommendation by physicians for women with PCOS. Lifestyle modifications including stress reduction, moderate exercise, and group support, along with a decrease in total calorie intake and avoiding junk food consumption have had positive results. A decrease of only 5% of total body weight is associated with decreased insulin levels, increased fertility, reduced hirsutism and acne, and lower testosterone levels. Whole grains, fruits and vegetables with foods containing protein and natural fat along with vitamins and minerals are beneficial
MORPHOLOGICAL STUDY AND MEDICINAL IMPORTANCE OF CLERODENDRUM INFORTUNATUM GAERTN. (VERBENACEAE), FOUND IN TADOBA NATIONAL PARK, INDIA
Full text linkClerodendron infortunatum Gaertn (Verbenaceae) is an important and widely used medicinal plant, reported to contain active bitter substance like clerodin, has been widely used as tonic and ant-helmintic agent in the countrysides of North India. Though, variously used in Ayurveda, Unani system of medicine and Homeopathy in case of ailments like diarrhea, skin disorders, venereal and scrofulous complaints, wounds, post-natal complications, as vermifuge, laxative and cholagogue, for the removal of ascarids in anus, as external applications on tumours, etc., the plant needs thorough investigation for its specific medicinal activity. Leaves and roots are used for external applications on tumours are due to the presence of Clerodolone, Clerodone, Clerodol and a sterol now designated as Clerosterol. Leaves and root extract is useful for the treatment of fresh wound. Percentage of haemoglobin increased due to leaf extract. Pile patient treated with leaf extract gives considerable relief. Sugar percentage reduced in diabetic patient. Slightly warm leaves applied with edible oil on the painful part of human body gives considerable relief within twelve hours. Seven sugars namely raffinose, lactose, maltose, sucrose, glactose, glucose and fructose were also identified from root extract. Pollen viability was assessed on the basis of hanging drop method. Reproductive success was assessed on the basis of fruit and seed se
QUANTITATIVE DETERMINATION OF ETORICOXIB AND PARACETAMOL IN PHARMACEUTICAL DOSAGE FORM AND IN-VITRO COMPARISON BY REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (RP-HPLC)
Full text linkThe objective of this present work was todevelop and validate analytical method for quantitativedetermination of Paracetamol and Etoricoxib in a tabletformulation and also the comparison of invitro data withreference dosage form. Chromatographic separations of thetwo drugs were analyzed on a Kromasil C18 column (25cmX 4.6mm, 5 m). The mobile phase constituted of Buffer:Acetonitirile with gradient program was delivered at theflow rate 1.0 mL/min. Detection was performed at 220 nm.Separation was completed within 20 min. Calibrationcurves were linear with coefficient correlation between0.99 to 1.0 over a concentration range of 48 to 146 g/mLof Paracetamol and 6 to 19 g/mL for Etoricoxibrespectively. The relative standard deviation (R.S.D) wasfound to be less than 2.0%. Analysis for dissolution studywas also performed by Reversed-Phase High PerformanceLiquid Chromatography (RP-HPLC) method. Differencefactor (f1) were found to be 2.85 and 3.83 and similarityfactor (f2) were found to be 73.514 and 68.961 forParacetamol and Etoricoxib respectively
A SURVEY ON METHODS OF UNDERGRADUATE PHARMACOLOGY TEACHING
Full text linkKnowledge of pharmacology to choose and prescribe drugs is a major challenge encountered by medical practitioners. A number of initiatives have been carried out to improve the teaching of pharmacology and applied therapeutics.Material & methods-A survey was conducted on medical students,pursuing pharmacology at Kasturba Medical College,Manipal,during the month of August 2010,to obtain information regarding students attitude towards Pharmacology. Result-Two hundred and fourteen students participated.The total median score was 56 (maximum score 80).Majority of them suggested to have more problem based learning than didactic lectures and to have integrated teaching with other clinical subjects. Suggestions to improve pharmacology teaching were noted.Conclusion-The findings of the study would be of interest to medical educators in modifying undergraduate pharmacology teaching programm
ANTIOVULATORY AND ESTROGENIC ACTIVITY OF LEAVES OF DATURA STRAMONIUM LINN. IN FEMALE ALBINO RATS
Full text linkThe effect of petroleum ether (60-8
ACTINOMYCIN “D” FROM MARINE SEDIMENT ASSOCIATED STREPTOMYCES CAPILLISPIRALIS MTCC10471
Full text linkIn our screening program for new bio-active metabolites from marine actinomycetes, a cyclic depsipeptide wasfound in the fermentation medium of marine Strepromyces (SS23/4) isolated from sediments collected from Bayoff Bengal, vellampattai,Tamilnadu. It showed strong biological activity against gram-positive / gram negativebacteria by agar overlay technique. It was taxonomically characterized by the basis of morphological andphenotypic characteristics, genotypic data and phylogenetic showing Streptomyces sps. Bio active compoundwas obtained by solvent extraction and purification using column chromatography followed by reverse phaseHPLC. The pure compound had potent activity against Mycobacterium tuberculosis and Multi Drug ResistantMycobacterium tuberculosis strains (437RU) at a concentration of 10 μg/mL, and The minimum inhibitoryconcentration (MIC) against standard test organisms was found to be 1μg/mL against B.subtilis, E.coli andMethicillin resistant Staphylococcus aureus. The compound exhibited potent cytotoxic activity against breastcarcinoma (MCF-7), melanoma cells (A375), prostate carcinoma (DU145) and lung carcinoma (A549) cellswith IC values 20μg/ml. The symbiotic Streptomyces capillispiralis MTCC 10471 produces crude antibiotic30mg/Lt by using nonoptimized fermentation conditions. The structure of the antibiotic was explained by 1D,2D NMR and LC-ESI-MS/MS, MALDI-TOF/MS experiments, revealed that it belongs to cyclic ploy peptideActinomycin D
ESTIMATION OF TIANEPTINE IN ITS TABLET FORM USING VISIBLE SPECTROPHOTOMETRIC METHODS
Full text linkTwo simple and sensitive visible spectrophotometric methods are described for the determination of tianeptine inbulk and pharmaceutical preparations based on the formation of colored species with SA- NaNO2