Università del Salento: ESE - Salento University Publishing

Messa, Francesco

Perrone, Serena

Milano, Francesco

Salomone, Antonio

Università del Salento - Coordinamento SIBA

LEbiotec VI edition – Lecce 27-29 September 2023 – Abstract Book 
 
45 
 
Extraction and Valorization of Antioxidant Polyphenols from Olive 
Leaves Using Environmentally Friendly Solvents 
Messa Francesco1, Perrone Serena1, Milano Francesco2, Salomone Antonio3 
 
1 Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Prov.le Lecce-
Monteroni, Lecce, I-73100, Italy 
2 Istituto di Scienze delle Produzioni Alimentari, Consiglio Nazionale delle Ricerche, UOS Lecce, Lecce, Italy 
3 Dipartimento di Chimica, Università degli Studi di Bari “Aldo Moro”, Consorzio C.I.N.M.P.I.S., Via Orabona, 4, 
Bari, I-70125, Italy 
 
 
 
Tyrosol (T) and hydroxytyrosol (HT) are olive-derived phytochemical polyphenols with a 
great biological activity related to their biological properties.1 In particular, HT exhibits 
numerous pharmacological activities and benefits for human health such as antioxidant, 
anti-inflammatory, anti-tumor, anti-viral, anti-bacterial and anti-fungal activities.2 Traditionally 
the extractions of these polyphenolic compounds have been carried out with Soxhlet 
techniques, using water or hydroalcoholic solutions as the solvent. However, the high 
temperatures reached in the process affect the thermolabile molecules of HT and T. 
Furthermore, due to the high affinity between water and polyphenols, the following 
purification step becomes complex. A valid alternative to classical extraction solvents was 
represented by Deep Eutectic Solvents (DESs), a promising class of eco-friendly extraction 
media composed by at least two constituents, which mixed in suitable stoichiometric ratios, 
generate a eutectic mixture liquid at room temperature.3  
In this communication, we present an eco-friendly solid-liquid extraction of T and HT from 
olive leaf as wastes, mediated by DESs as solvents. The use of DES favors a very selective 
and environmentally friendly extraction, preserving the stability of the polyphenols. 
Moreover, to valorize the polyphenols extracted, they were subjected to a “green” 
alkoxycarbonylation reaction, using Mo(CO)6 as a solid and safe to handled CO source.4,5 
The methodology was carried out in cyclopenthyl methyl ether (CPME) as a bio-derived 
reaction media (Scheme 1). The antioxidant properties of the novel synthetized ester 
derivatives were also assessed. 
 
 
 
 
 
LEbiotec VI edition – Lecce 27-29 September 2023 – Abstract Book 
 
46 
 
 
 
Scheme 1. Ecofriendly extraction of tyrosol (T) and hydroxytyrosol (HT) from olive leaves 
with DESs and their “green” alkoxycarbonylation in the bio-derived CPME. 
 
 
 
 
 
References 
 
[1] A.E. Ünlü, Waste Biomass Valorization 2021, 12, 5329–5346. 
[2] M. Bertelli, A.K. Kiani, S. Paolacci, E. Manara, D. Kurti, K. Dhuli, V. Bushati, J. Miertus, D. 
Pangallo, M. Baglivo, T. Beccari, S. Michelini, J. Biotechnol. 2020. 29–33. 
[3] E.L. Smith, A.P. Abbott, K.S. Ryder, Chem Rev 2014, 114, 11060–11082. 
[4] F. Messa, A.N. Paparella, S. Perrone, A. Salomone, Org Biomol Chem 2023, 21, 5164–
5170. 
[5] F. Messa, A.N. Paparella, D. Veselý, J. Krajčovič, P. Papadia, S. Perrone, A. Salomone, 
European J Org Chem 2023, 26, e2023003. 
  


English

Extraction and Valorization of Antioxidant Polyphenols from Olive Leaves Using Environmentally Friendly Solvents

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Extraction and Valorization of Antioxidant Polyphenols from Olive Leaves Using Environmentally Friendly Solvents

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