Poltekkes Kemenkes Kupang Online Journal
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Implementasi Implementation Of Health Care Tasks Throughfamily Counseling For Tuberculosis Patients At The Sikumana Community Health Center
No full textTuberculosis (TB) is an infectious disease with a high number of cases in Indonesia, including in the working area of the Sikumana Community Health Center. Low patient compliance in undergoing therapy is one of the main challenges. Families play an important role in accompanying patients, from monitoring medication intake and providing nutrition to giving emotional support. To determine the effect of implementing health care tasks through family support on tuberculosis patients at the Sikumana Community Health Center. Quantitative research with a pre-experimental one-group pretest-posttest design. The research sample consisted of 30 respondents who were family members of tuberculosis patients. The instruments used were a family support questionnaire covering five family health tasks and a SAP on Family Support. Data analysis was performed using the Wilcoxon test. Results: In the pretest, most families were in the adequate (53%) and poor (46.7%) categories, with none in the good or very good categories. After the intervention, the posttest results showed a significant improvement, with 60% of respondents in the good category and 20% in the very good category. The Wilcoxon test produced a p-value < 0.001, indicating a significant effect of family support on the improvement of health care tasks. Family support was proven to increase family involvement and understanding in supporting TB patient treatment. Suggestions: Family support needs to be made a sustainable strategy in primary care, with a broader sample coverage and innovative interactive educational media to increase effectiveness
Extract of Teak Leaf Buds (Tectona grandis) as Natural Dyes Replacement of Eosin on HE Staining
No full textHematoxylin and Eosin (HE) staining is a routine method in histopathology. In HE staining, eosin functions to stain the cytoplasm of the tissue. Continuous use of eosin may cause health problems due to its carcinogenic properties, thus requiring an alternative. One such alternative is teak leaf buds, which contain anthocyanin compounds. The aim of this study was to determine whether teak leaf bud extract can be used as a substitute for eosin in HE staining. The type of research used was a true experimental study with a post-test only control group design. The samples consisted of paraffin blocks, from which 10 slides were prepared: one control and nine treatment slides, with three repetitions for each concentration (20%, 30%, and 40%). Microscopic observations of HE staining using eosin showed a score of 3 (100%). Using 20% teak bud extract resulted in a score of 2 (100%), 30% concentration gave a score of 2 (33,3%) and score 3 (66,7%), while 40% concentration resulted in score 3 (100%). Kruskal-Wallis test showed eosin and teak leaf bud extract were not significantly different: for 20% concentration, p=0,083; for 30%, p=0,564; and for 40%, p=1,000 (p>0,05), indicating no significant difference.It can be concluded that 40% teak leaf bud extract is the best concentration and has the potential to replace eosin in HE staining. Future research is suggested to use 40% teak leaf bud extract with variations in staining time to further explore its potential as a substitute for eosin in histological staining
Maternal and Child Determinants of Psychological Well-Being in Mothers Caring for Children with Chronic Conditions
Full text linkCaring for a child with a chronic condition imposes significant physical and psychological burdens on mothers, including increased stress, anxiety, depression, and difficulties balancing daily caregiving responsibilities with personal and social roles, which negatively impact their overall psychological well-being. This study aims to analyze the factors (maternal and child characteristics) associated with the psychological well-being of mothers who care for children with chronic conditions. A cross-sectional correlation design was used with 204 mothers of chronically ill children at Hospital in Malang City, selected through purposive sampling. The research instruments in this study are a questionnaire. Data analysis was performed using ordinal logistic regression. A p-value of less than 0.05 was considered statistically significant. Most mothers have moderate psychological well-being (72,1%). Multivariate analysis showed that mothers aged 26–35 years (OR = 0.113, p = 0.020), treatment duration under 1 year (OR = 5.551, p = 0.001), living in a village (OR = 0.221, p = 0.009), high family income (reference group, OR = 1.00), low obstacles to care (OR = 5.948, p = 0.009), good partner support (OR = 16.609, p = 0.002), good family support (OR = 6.062, p = 0.031), and good health worker support (OR = 6.965, p = 0.036) were significantly associated with increased maternal psychological well-being. Health workers can actively provide psychosocial support for mothers caring for children with chronic conditions, such as counseling. In addition, health workers must work with partners and families to create an emotionally supportive environment for mothers.
The Reappearance of Menstruation After Childbirth Associated with Contraceptive Use Among Women of Reproductive Age in Kyrgyzstan
Full text linkThe return of menstruation after childbirth is an important aspect of women\u27s reproductive health, especially in the context of contraceptive use. This study aims to explore how the return of menstruation after childbirth is closely connected to contraceptive use, with various factors such as age, marital status, region, place of residence, education level, ethnicity, and wealth index in Kyrgyzstan playing a role. This study employed secondary data from the Kyrgyzstan 2023 Multiple Indicator Cluster Survey (MICS), focusing on women of reproductive age (15-49 years). The analysis utilized a final sample of 1.059 women and applied binary logistic regression to examine the relationship between contraceptive use and the return of menstruation after childbirth while controlling for factors such as age, marital status, region, education level, ethnicity, and wealth index. The study found that among 1.059 women who had given birth, 30.88% were using contraceptive methods, and 64.68% experienced the return of menstruation post-childbirth. The multivariate analysis indicates that menstrual return after giving birth, having marital status formerly married/in union, in Naryn, Talas, and Chui region, and having the richest wealth index significantly influence contraceptive use among women of reproductive age. These results underscore the importance of comprehensive sexual health education, improved healthcare services, and targeted communication strategies to increase contraceptive use in the region.
The Activity of Older People Determining Their Functional Abilities During Old Age: A Cross-Sectional Study in Two Major Cities In Indonesia
Full text linkAmidst Indonesia\u27s aging population, this study explores key determinants influencing productivity, the indicators of functional abilities, among older adults in diverse urban/rural settings of East Java and Yogyakarta. The study design used a cross-sectional study surveyed 220 adults (≥60 years; mean age 65.3) via stratified probability sampling. Structured questionnaires assessed holistic health (biological, psychological, social, spiritual, cultural), active ageing determinants (social, habitual, physical, personal, economic, service access), and elderly capability improvement (physical, mental, social, spiritual, cultural health). Binary logistic regression identified predictors of productivity status. The study results showed that holistic health conditions (Exp(B) = 0.983, p = 0.050), active ageing determinants (Exp(B) = 1.16, p =0.000), and improvements in elderly capabilities (Exp(B) = 1.090, p = 0.005) were significant positive predictors of productivity. This Indonesian study demonstrates that specific active ageing determinants and enhanced capabilities are more critical to elderly productivity than general holistic health in a multivariate context. It highlights the need for targeted, regionally adaptable community interventions and age-friendly policies to support productive aging.
The Role of Alcohol in the DNA Isolation Process: A Comprehensive Review
Full text linkDNA isolation is a fundamental step in various molecular biology applications, with its success highly dependent on the purity and integrity of the isolated DNA. This review article aims to highlight the vital role of alcohol, particularly ethanol and isopropanol, in the DNA isolation process from various biological sources. This comprehensive review explores the biophysical principles underlying DNA precipitation by alcohol, where the reduction of the solution\u27s dielectric constant and disruption of the hydration layer lead to DNA precipitation. The article also evaluates the application of alcohol in different DNA isolation protocols, including the classic phenol-chloroform extraction method, commercial silica column-based kits, and salting-out techniques. Recent advancements in optimizing alcohol precipitation parameters indicate that process efficiency can be enhanced by adjusting the type of alcohol, incubation time, and salt concentration. Current research trends also point toward the development of environmentally friendly approaches that minimize alcohol usage, as well as alternative alcohol-free methods such as magnetic-based technology and paper-based DNA isolation systems.The future prospects of DNA isolation are projected to integrate traditional alcohol-based methods with advanced technologies such as automation, nanomaterials, and microfluidic systems. A deeper understanding of alcohol’s role in DNA isolation is expected to optimize existing protocols and drive innovation in DNA isolation techniques for applications ranging from diagnostics to genomic research
Pengaruh substitusi tepung ikan lele (Clarias sp) dan tepung kacang hijau (Vigna radiata) terhadap daya terima biskuit
Full text linkTo achieve optimal growth and development, it is important to consider the golden age, often referred to as the vital development phase for children. According to the 2023 Indonesian Health Survey (SKI), 9.4% of children in East Nusa Tenggara (NTT) province are underweight. Therefore, researchers decided to investigate the impact of replacing catfish flour with green bean flour on biscuit acceptance. The objective of this study was to determine the nutritional components and biscuit acceptance test of biscuits made using green bean flour as a substitute for catfish flour. This study is an experimental study, with food formulations using proportions of catfish flour (10%, 20%, 30%) and green bean flour (30%, 20%, 10%). Acceptance test data were analyzed descriptively, using the Normality Test, ANOVA, and Tukey\u27s Test. It can be concluded that the preference level for each treatment received a score of 4, indicating preference. Based on statistical analysis, there was a significant difference in taste between treatments P0 and P3. The macronutrient values of the biscuits, with varying proportions of catfish flour to green bean flour, were affected (energy, protein, fat, and carbohydrates). To prevent cross-contamination from the environment, researchers are advised to use an oven when drying the green beans and catfish, and to use uniform molds to ensure consistent product weight.Untuk mencapai pertumbuhan dan perkembangan yang optimal, penting untuk mempertimbangkan masa keemasan yang sering dikenal sebagai fase perkembangan vital bagi anak-anak. Menurut Survei Kesehatan Indonesia (SKI) 2023, anak yang kekurangan berat badan di provinsi Nusa Tenggara Timur (NTT) mencapai 9,4%. Oleh karena itu, peneliti memutuskan untuk meneliti dampak penggantian tepung ikan lele dengan tepung kacang hijau terhadap penerimaan biskuit. Tujuan dari studi ini adalah untuk mengetahui komponen gizi dan tes penerimaan biskuit yang dibuat dengan menggunakan tepung kacang hijau sebagai pengganti tepung ikan lele. Studi ini merupakan studi eksperimental, formulasi makanan menggunakan proporsi tepung ikan lele (10%, 20%, 30%) dan tepung kacang hijau (30%, 20%, 10%). Data tes penerimaan dianalisis secara deskriptif, dengan melakukanTes Normalitas, ANOVA dan Tes Tukey. Dapat disimpulkan bahwa tingkat kesukaan untuk setiap tindakan mendapatkan skor 4 yang menunjukkan kesukaan. Berdasarkan analisis statistik, terdapat perubahan rasa yang relevan antara tindakan P0 dan P3. Nilai makronutrien biskuit proporsi tepung ikan lele terhadap tepung kacang hijau memengaruhi (kekuatan, protein, lemak, dan karbohidrat). Untuk mencegah kontaminasi silang dari lingkungan, peneliti selanjutnya diharapkan menggunakan oven saat mengeringkan kacang hijau dan ikan lele, menggunakan cetakan yang seragam untuk menjamin berat produk
Dietary Intake and Pregnancy Characteristics on Maternal Anemia Status
No full textAnemia during pregnancy remains a significant global health concern, affecting a substantial portion of the worldwide population. The prevalence of anemia is particularly high among pregnant women, especially in developing countries like Indonesia. Anemia during pregnancy can lead to severe complications for both mother and fetus, including increased mortality risk, premature birth, low birth weight, and cognitive impairment. Purpose: This study aimed to identify factors contributing to anemia, specifically examining food intake and pregnancy characteristics. A cross-sectional study was conducted from June to September 2023 at Parung Panjang Health Center, Bogor Regency, involving 135 pregnant women. Data collection included hemoglobin measurements using an easy touch device and nutritional intake assessment through SQ-FFQ interviews. Analysis was performed using SPSS version 23 with chi-square testing. The study revealed significant correlations between parity, intake of calcium, and vitamin C with anemia status (p<0.05). Most participants were in their third trimester, predominantly unemployed, and had low education levels. There was no significant association between nutrition status, age, family income and infection story with anemia status (p>0.05). While the majority of women had adequate iron intake, no significant association was found between iron intake and anemia status (p>0.05). The study identified parity, intake of calcium, and vitamin C as significant factors associated with anemia in pregnant women. These findings emphasize the importance of comprehensive nutritional support and monitoring during pregnancy, particularly focusing on adequate micronutrient intake and considering parity status in anemia prevention strategies.
Mercury Exposure in Pregnant Women Using Whitening Cosmetics and Potential Health Risks
No full textRecently, women are increasingly using whitening cosmetics to beautify their skin. However, they are not aware that whitening cosmetics contain mercury. Several studies have reported high levels of mercury in skin whitening creams. Women, especially pregnant and lactating mothers, who use these creams are at risk of mercury poisoning because long-term exposure can cause permanent nerve damage, kidney disorders, fertility problems, and congenital disabilities. This study aimed to analyze mercury exposure in pregnant women using whitening cosmetics and potential health risks. This research method is an observational analytic, cross-sectional design. The instrument is a questionnaire. Sampling was carried out at Khadijah 1 Hospital, Makassar City. The sample in this study consisted of 20 pregnant women who used whitening cosmetics, 20 who did not use whitening cosmetics and 20 samples of whitening cosmetics used by pregnant women. The cosmetic samples analyzed were the concentration of mercury contained in the cosmetics. Mercury testing was conducted at The Indonesian Institute for Public Health Laboratories, Makassar (BBLK). Data analysis using SPSS software. This result study showed that 20 cosmetics used by pregnant women were detected with mercury. The highest mercury concentration was 86.1 ppm, and the lowest was 3.5 ppm. Those registered with the Food and Drug Administration (BPOM) were 11 cosmetics, and 9 were not registered with BPOM. Statistical analysis shows a correlation between the frequency, duration, and volume of cosmetic use and mercury concentration in pregnant women\u27s hair, namely 0.008, 0.017, and 0.032, respectively. The potential health risks experienced by pregnant women who use cosmetic whitening creams are anemia of as much as 55%, the fetus not actively moving as much as 15%, and the weight of the fetus not increasing as much as 30%. The statistical analysis of the relationship between mercury exposure and potential health risk experienced by pregnant women, with a p-value of 0.039 < p-value of 0.05. The use of whitening cosmetics in pregnant women can have an impact on the fetus in the womb. Therefore, pregnant women should not use cosmetics containing mercury during pregnancy and should increase their knowledge about the contents of cosmetics
PENAMBAHAN DAUN BAYAM MERAH TERHADAP SIFAT ORGANOLEPTIK DAN NILAI GIZI SOSIS IKAN CAKALANG GUNA MENCEGAH ANEMIA PADA REMAJA
No full textParenting is a pattern Anemia is a condition where the hemoglobin level in the body is less than normal. In general, adolescent girls are susceptible to anemia due to inadequate nutritional intake, physical activity and menstruation. Normal Hb levels in adolescent girls are >12 g/dL. Adolescent girls need more iron than adolescent boys, because adolescent girls experience menstruation every month, adolescent girls who experience menstruation for a long period of time will cause anemia. One way to prevent anemia is by providing functional food ingredients that have been developed, namely adding red spinach leaves to skipjack fish sausages, where red spinach has a high iron content which can overcome anemia in young women. The research objective: to determine the organoleptic tests and nutritional content of fish sausages with the addition of red spinach leaves. Research Method: The research used a completely random method (RAL). With the addition of red spinach leaves, there were 4 treatments where P1 10% P2 15% and P3 20% Results: The results of the study showed that the comparison of skipjack tuna meat with the addition of red spinach leaves had an effect on descriptive and hedronic sensory assessments of color and taste attributes. Conclusion: The best organoleptic test results and the panelists liked the most was P2 with the addition of 52 grams of red spinach leaves = 15%. From the research results the author recommends P3: 20% because it has the highest nutritional content. Values in nutritional analysis per slice of 41 pieces: energy 22.27 kcal, protein 1.96 grams, fat 0.26 grams, carbohydrates 2.98 grams, iron 0 .51 mg.Anemia adalah suatu keadaan dimana kadar hemoglobin dalam tubuh kurang dari normal. Pada umumnya anemia rentan terjadi pada remaja putri karena asupan gizi yang kurang, kegiatan fisik dan menstruasi. Kadar Hb normal pada remaja putri adalah >12 g/dL. Remaja putri lebih banyak membutuhkan zat besi dari pada remaja putra, dikarenakan remaja putri mengalami mensruasi setiap bulannya, remaja putri yang mengalami menstruasi dalam kurun wakatu yang lama akan menyebabkan anemia. Salah satu pencegahan anemia dengan pemberian bahan pangan fungsional yang dikembangkan yaitu penambahan daun bayam merah pada sosis ikan cakalang, dimana bayam merah memiliki kandungan zat besi yang tinggi yang dapat mengatasi anemia pada remaja putri. Adapun Tujuan Penelitian : untuk mengetahui uji organoleptik dan kandungan gizi pada sosis ikan dengan penambahan daun bayam merah.Metode Penelitian : Penelitian menggunakan metode acak lengakp (RAL). Dengan penambahan daun bayam merah sebanyak 4 perlakuan dimana P1 10% P2 15% dan P3 20% Hasil : Hasil penelitian menunjukan bahwa perbandingan daging ikan cakalang dengan penambahan daun bayam merah berpengaruh terhadap penilain deskriptif dan sensori hedronik terhadap atribut warna dan rasa.Kesimpulan : Hasil uji organoleptik yang terbaik dan paling disukai panelis adalah P2 dengan penambahan daun bayam merah sebanyak 52 grm = 15%. Dari hasil penelitian penulis merekomendasikan P3 : 20% karena memiliki kandungan gizi tertinggi, Nilai pada analisis nutrisi perpotong 41 buah: energi 22,27 kkal, protein 1,96 grm, lemak 0,26 grm, karbohidrat 2,98 grm, zat besi 0,51 mg