International Journal of Advances in Medical Biotechnology (IJAMB)
Not a member yet
91 research outputs found
Sort by
Fluid flow in a Porous Scaffold for Microtia by Lattice Boltzmann Method
Get PDFThe birth deformity of ear, known as microtia, varies from a minimal deformed ear to the absence of auricular tissue or anotia. This malformation has been treated by reconstructing the external ear, mainly by autogenous rib cartilage in auricular repair. The fabrication of the ear framework is a prolonged reconstructive procedure and depends of the surgeon’s skill. In order to avoid these inconveniences and reduce surgery time, it was proposed in a previous work to use implants made with biocompatible materials. One of these is a scaffold made by fused deposition modeling using PLA based in the three-dimensional geometry of the ear cartilage. The aim of this work is to evaluate the feasibility of this scaffold to perform cell culture in a perfusion biorreactor by estimating the flow transport characteristics in porous media using a scaffold with the porous geometry of the human auricular cartilage for microtia. Flow and heat transfer through the scaffold were simulated by the lattice Boltzmann method, and permeability and shear stress distribution were obtained at different Reynolds numbers. The permeability values of the scaffold achieved are in the order of magnitude of scaffolds used for cell culture. Linear dependencies between maximum shear stress and Reynolds number, and between maximum shear stress and permeability were obtained. The values of shear stress achieved correspond to high percentage of cell viability. The scaffolds for microtia treatment with the proposed filling pattern select is appropriate for cell culture in a perfusion bioreactor with characteristics similar to those described herein
3D electrospinning used in medical materials
Get PDFElectrospinning (ES) is an interesting and efficient technique for biomedical use. This is a method used for the fabrication of polymer fibers used in tissue engineering (TE). The electrospun nano- and microfibers biomaterial, called scaffolds, are also used for regenerative medicine. The aim of the present mini-review is to present methods used to fabricate 3D fibers by electrospinning and their applications in TE. Also, discussed here are issues regarding the electrospinning limitations and research challenges
Intelligent copolymers based on poly (N-isopropilacrylamide). Part ii: Grafts polysaccharide to obtain new biomaterials for biomedical and pharmacological applications
Get PDFBiopolymers such as polysaccharides are compounds that have functional groups and they are very susceptible to be used in chemical modifications and also allows them to synthesizer of new copolymers (used as graft-like chains). Poly (N-Isopropylacrylamide) PNIPAm, is a thermosensitive synthetic polymer widely used in the preparation of intelligent gels for the biomedical field, but have some limitations in use as biodegradable matrix or scaffolds. In this research wered the synthesis and characterization of copolymers their PNIPAm grafted with the polysaccharides: chitosan (CS) or hyaluronic acid (HA), were performed to obtain new biodegradable and biocompatible biomaterials that conserve the intelligent character (thermosensitivity).The PNIPAm was in first chemically modified with 3-butenoic acid in order to generate carboxyl end groups on the graft-polymer chain (PNIPAm-co-COOH) which serve as anchor points and then covalently graft the polysaccharides. For the specific case of grafting with hyaluronic acid, it was necessary to perform a second modification using piperazine (PIP) and obtain the graft-polymers PNIPAm-co-COO-g-PIP. All this modification process was previously reported (Carrero et al, 2018). In this case, the polysaccharides used as grafts-like chains were: (1) chitosan oligomers obtained by acid degradation and (2) hyaluronic acid. The characterization of all copolymers obtained was follow by infrared spectroscopic (FT-IR); the differential scanning calorimetric (DSC) technique was used to determine the lower critical solution transition temperature (LCST), resulting in the range of 29-34 °C. Its morphology was studied using scanning electron microscopy (SEM), but previously was simulate an inject process, for the reversible gel character presented by these novel copolymers; resulting a high porosity and interconnection between pores (scaffold-like micrometric structures). Hemocompatibility assays were performed on agar/blood systems, showing non cytotoxicity. All these results give these graftcopolymers a high potentiality of use as scaffolds in tissue engineering and also for pharmacological applications
Tissue engineering of different cartilage types: a review of different approaches and recent advances
Get PDFCartilage is a connective tissue that serves as a structural support for maintaining the shape for specific appendices (nose, ear) and also helps for shock absorption when present in joints. Different types of cartilage coexist in the body: hyaline, elastic and fibrocartilage. Due to their different embryologic origin, they produce distinct extracellular matrix and therefore have specific functions according to their location. Cartilage is frequently subjected to many different lesions. Those include traumatic, metabolic and congenital forms, concerning all regions where this tissue is present: joints, head and neck area, intervertebral disks, etc. Increasing number of cancers also affects cartilage; especially in ear, nose and trachea. Unfortunately, this tissue has a poor regeneration ability. Few therapeutic options exist for cartilaginous lesions and most of them concern articular cartilage. They include micro fracture, autologous chondrocytes implantation, mosaicplasty, allograft and prosthesis. Ear and trachea are also targeted for reconstruction with lesser extent. Therefore, cartilage engineering highly addresses increasing number of pathologies associated to this tissue. In the last two decades, several trials were investigated using both progenitor cells and scaffolds. Even bone marrow derived stem cells were widely used and served as gold standard. Many progenitors from different areas are investigated for their capacity of chondrogenesis. On the other hand, biomaterials, natural and synthetic, are used to induce a 3D environment that allows proper growth and differentiation toward cartilage formation. Their characteristics depend on the location of the expected graft where porosity, biodegradability, ability to support strength and large scale use are the key points. Favorable environments are also needed to achieve appropriate chondrogenesis, including biochemical or mechanical stimuli and low oxygen tension. Bioprinting showed also encouraging outcomes in cartilage reconstruction with the investigation of several scaffolds
The importance of Biotechnology in the health area: historical overview and future perspectives
Get PDFA biotecnologia, em sentido amplo, compreende a manipulação de microrganismos, plantas e animais, com a proposta de obter processos e produtos de interesse da sociedade. Embora o termo tenha surgido no início do século XX, os processos biotecnológicos estão presentes desde os tempos antigos na produção de alimentos e bebidas fermentados. A biotecnologia também está presente desde o início da medicina. A inalação de pó de feridas de varíola humana secas ao sol ou a secreção do úbere de vacas contaminadas com varíola bovina são exemplos de técnicas antigas usadas como métodos de imunização da doença. Nesse sentido, muitos tratamentos surgiram ao longo do tempo, como vacinas, produção de penicilina, o primeiro antibiótico e produção de insulina por DNA recombinante, que revolucionaram a Medicina e possibilitaram uma melhor qualidade de vida para os seres humanos. Muitos produtos e materiais da Biotecnologia ainda estão surgindo, como andaimes em Medicina Regenerativa para recuperação de tecido ósseo, tubos de celulose bacteriana usados como vasos sanguíneos artificiais, células-tronco pluripotentes induzidas e sistemas microfisiológicos, que prometem melhorar ainda mais a medicina moderna e fornecer melhores técnicas e técnicas. terapias para vários tratamentos
Special session in honor of Antonio Carlos Massabni
Get PDFThe present issue of International Journal of Advances in Medical Biotechnology (IJAMB) introduces a set of five papers elaborated by Prof. Antonio Carlos Massabni and students that have attended a graduate course nominated Seminars of Integration from the Postgraduate Program in Biotechnology of the University of Araraquara (UNIARA), Araraquara, Brazil. The authors have focused on the impact of Industry 4.0 technologies on Biotechnology as well as the chance for future professionals and the work relations in this context. In other papers, the importance of biotechnology in the development of vaccines, antibiotics, hormones and scaffolds for regenerative medicine are discussed. This issue is dedicated in honor of Prof. Massabni for his commitment with teaching, research and administrative duties
Bacterial cellulose-based biomaterials on third-degree burns in rats
Get PDFBurns are cutaneous lesions that present high rate of morbidity and mortality worldwide. In order to innovate the treatment strategies currently applied new biomaterials are being investigated. The aim of the present study was to evaluate the action of bacterial cellulose in both membrane and gel form, in the treatment of third degree burns in rats. For this, 24 Wistar rats were used, divided into three distinct groups. The lesion was performed with the aid of a soldering iron heated at 150 °C pressed on the back of the animal for 10 seconds. Treatment was performed immediately after wound induction, and skin samples were collected on the tenth day post-injury. Statistical analysis was performed using a significance level of 5% (p?0.05). The histological results show differences in the healing process presented by each group. The group that received bacterial cellulose in the membrane format presented the best results, such as discrete inflammatory infiltrate and better morphological quality of the tissue, characterizing an advanced stage of the healing process, also proven in the collagen quantitative analysis. On the other hand, the group that received the cellulose gel showed characteristics of an inflammatory phase with the presence of evident ulcerations, which corresponds to a delay in the healing process even when compared to CG alone. Thus, it was concluded that before the biomaterials tested cellulose membrane in the format presented more favorable results both in terms of environmental protection as a contribution to an adequate tissue recovery.
1st Brazilian Meeting of Biocellulose University of Araraquara - UNIARA June 4 and 5, 2018
No full textBrazilian Meeting of Biocellulose ", promoted by the University of Araraquara - Uniara, through the Biopolymers and Biomaterials Laboratory - BioPolMat of its Graduate Program in Biotechnology in Regenerative Medicine and Medicinal Chemistry - PPGB-MRQM, and by the Company's units Brasileira de Pesquisa Agropecuária - Embrapa of Fortaleza and São Carlo
Editorial
Get PDFIJAMB had its beginning counting on a highly qualified team in different areas of knowledge which will be of great value in terms of improving impact and novelty related to the publications.This journal aims to be a vehicle for disseminating research, advances and developments in the field of medical biotechnology, with emphasis in medicinal chemistry, bioelectronics, biophotonics, biopolymers, 3D printing for biomedical applications, tissue engineering and regenerative medicine areas. In addition, the submission of new methodologies that support studies within the above mentioned areas are also welcome. Our Publishing Process is based on the most relevant journals related to the topics covered by IJAMB, which periodicity at this first moment will be biennial. We are pleasure to invite colleagues according to the mentioned areas of interest to submit their articles for analysis in our editorial peer-reviewed system which will certainly allow an evaluation with ethics and criteria. It is known that there is a large number of "predatory" magazines that reduce quality in cientific publishing and some charge for their publications. This magazine will be the "online" and "open access" type, making the articles visible and available for download at any time in any part of the world. IJAMB is already in English language format, so that in a short time the magazine will be internationally recognized, allowing a new range of collaborators and referees, as well as new members to the editorial staff. Therefore, we are very hopefull that all members envolved and future ones will really strive to contribute to the evolution of this publication which quality and impact of the shared information will certainly increase.Welcome to the International Journal of Advances in Medical Biotechnology
Morphology study of alginate micro/nano particles for the encapsulation of divalents Mg2+ AND Zn2+ IONS
Get PDFThis research work aimed to promote the formation of alginic acid particles and the encapsulation of divalent ions, such as Zn2+ and Mg2+; but using a combination of internal alginate gelation and micro-emulsification method. Both ions are essential elements of the human body, i.e., they are present in tissues and body fluids and participates in many bodily functions. The influence of different parameters was evaluated relate to the formation of the particles in micro/nano-scale, and their morphology was observed. The concentration of both ions used in the formulation was varied considering [0.075, 0.15 and 0.25] mol/L. It was found in general that the formation of particles in nanoscale, with a spherical shape and smooth surfaces (also by Atomic force microscopy AFM) after characterizing by electron microscopy (Scanning SEM and Transmission TEM) with energy-dispersed analysis of X-rays (SEM/EDX). The only evidence of formation of particles at higher concentrations of the ion ([0.25] mol/L) was found when the magnesium ion was used (MgSO4) while the smallest particles (?100nm) were formed when ZnSO4 ([0.25] mol/L) was used. The results suggest that these particles can be used as a coat or carrier for essential nutrients for food fortification, for instance, for others applications in biomedicine or charge drugs in delivery systems