1401 research outputs found
Sort by
Toolkit: Waste Flow Diagram (WFD): A rapid assessment tool for mapping waste flows and quantifying plastic leakage. Version 1.0.
No full textThe Waste Flow Diagram toolkit is an excel-based model allowing users to perform a rapid assessment of municipal solid waste flows within a city or municipality. The tool maps these waste flows using Sankey diagrams and quantifies the plastic waste that is emitted from the solid waste management system and its eventual fate in the environment. The toolkit is specifically designed to integrate with SDG 11.6.1 by informing on the sub-indicators, and can be operated either as a baseline assessment or used for scenario forecasting
Exploring high aspect ratio gold nanotubes as cytosolic agents: structural engineering and uptake into mesothelioma cells - dataset
Get PDFThe generation of effective and safe nanoagents for biological applications requires their physicochemical characteristics to be tunable, and their cellular interactions to be well characterized. Here, the controlled synthesis is developed for preparing high‐aspect ratio gold nanotubes (AuNTs) with tailorable wall thickness, microstructure, composition, and optical characteristics. The modulation of optical properties generates AuNTs with strong near infrared absorption. Surface modification enhances dispersibility of AuNTs in aqueous media and results in low cytotoxicity. The uptake and trafficking of these AuNTs by primary mesothelioma cells demonstrate their accumulation in a perinuclear distribution where they are confined initially in membrane‐bound vesicles from which they ultimately escape to the cytosol. This represents the first study of the cellular interactions of high‐aspect ratio 1D metal nanomaterials and will facilitate the rational design of plasmonic nanoconstructs as cytosolic nanoagents for potential diagnosis and therapeutic applications
Sphingomyelinase Disables Inactivation in Endogenous PIEZO1 Channels
No full textEndogenous PIEZO1 channels of native endothelium lack the hallmark inactivation often seen when these channels are overexpressed in cell lines. Because prior work showed that the force of shear stress activates sphingomyelinase in endothelium, we considered if sphingomyelinase is relevant to endogenous PIEZO1. Patch clamping was used to quantify PIEZO1-mediated signals in freshly isolated murine endothelium exposed to the mechanical forces caused by shear stress and membrane stretch. Neutral sphingomyelinase inhibitors and genetic disruption of sphingomyelin phosphodiesterase 3 (SMPD3) cause PIEZO1 to switch to profoundly inactivating behavior. Ceramide (a key product of SMPD3) rescues non-inactivating channel behavior. Its co-product, phosphoryl choline, has no effect. In contrast to ceramide, sphingomyelin (the SMPD3 substrate) does not affect inactivation but alters channel force sensitivity. The data suggest that sphingomyelinase activity, ceramide, and sphingomyelin are determinants of native PIEZO gating that enable sustained activity
Dataset associated with "Friction between soft contacts at nanoscale on uncoated and protein-coated surfaces"
No full textThe understanding of friction on soft sliding biological surfaces at the nanoscale is poorly understood as hard interfaces are frequently used as model systems. Herein, we studied the influence of elastic modulus on the frictional properties of model surfaces at the nanoscale for the first time. We prepared model silicone-based elastomer surfaces with tuneable modulus ranging from hundreds of kPa to a few MPa, similar to those found in real biological surfaces, and employed atomic force microscopy to characterize their modulus, adhesion, and surface morphology. Consequently, we used friction force microscopy to investigate nanoscale friction in hard-soft and soft-soft contacts using spherical colloidal probes covered by adsorbed protein films. Unprecedented results from this study reveal that modulus of a surface can have a significant impact on the frictional properties of protein-coated surfaces with higher deformability leading to lower contact pressure and, consequently, decreased friction. These important results pave the way forward for designing new functional surfaces for serving as models of appropriate deformability to replicate the mechanical properties of the biological structures and processes for accurate friction measurements at nanoscale
Pt/CoB/Pt Josephson π-junctions with perpendicular magnetic anisotropy
No full textThis is the dataset for "Pt/CoB/Pt Josephson π-junctions with perpendicular magnetic anisotropy" containing raw electrical transport and magnetization data appearing in the main tex
Analysis of the Polysemy Exhibited by Spatial Prepositions
No full textIn previous work exploring the semantics of spatial prepositions in grounded settings, we conducted a study to gather annotations related to spatial language in 3D virtual environments (https://doi.org/10.5518/764). Using this collected data we initially considered a semantic model based on Prototype Theory and introduced a method for learning its parameters from data which we discuss in http://eprints.whiterose.ac.uk/156866/. However, though there is much to suggest that spatial prepositions exhibit polysemy, each term was treated as exhibiting a single sense. The ability for terms to represent distinct but related meanings is unexplored in the work on grounded semantics and referring expressions, where even homonymy is rarely considered. Using the previously collected data we have examined how the polysemy exhibited by spatial prepositions may be incorporated into semantic models for situated dialogue and we have tested the approaches in a reference task. The current dataset provides an archive of the associated code and results of this analysis
Polyelectrolyte Complex Templated Synthesis of Monodisperse, Sub-100 nm Porous Silica Nanoparticles for Cancer Targeted and Stimuli-Responsive Drug Delivery - Dataset
No full textThis dataset provides the data used to create graphs throughout the manuscript ‘Polyelectrolyte Complex Templated Synthesis of Monodisperse, Sub-100 nm Porous Silica Nanoparticles for Cancer Targeted and Stimuli-Responsive Drug Delivery’
Changes in food intake to reduce CO2 emissions and have a healthy diet
Get PDFIntroduction: Human activities have resulted in global warming by high CO2 emissions. Poor dietary choices are linked to health diseases and high CO2 emissions. Most studies focus on the changes the average population diet, often suggesting radical changes to our diets.
Methodology: An optimisation algorithm will be created to minimise CO2 emissions without changing greatly the current food intake of each individual. Diets of 212 individuals will be used, along with CO2 emissions for each food item in the sample.
Results: Current diets contribute 6.94kg of CO2 emissions per person per day, which was reduced by 30% after the optimisation. 100 out of 212 individuals had an optimised diet as the result (47%). Meat and dairy contribute significantly more in CO2 emissions from diets compared to fruits and vegetables. Meat, dairy products, fruits and vegetables are 12% of our food intake each, however after the optimisation, meat and dairy were decreased by 23% whereas fruits and vegetables were increased by 30% and 4% respectively.
Conclusions: Optimising food intake led to a healthy diet low in CO2 emissions by combining foods in current diets. It is possible to achieve this by making small changes to the food intake consumed
Chalumeau venting study no.1
Get PDFA study for solo clarinet on 'venting'; the technique of altering a clarinet fingering by opening holes further up the instrument (skipping at least one closed hole above the terminator hole), which sharpens pitch, can afford register shifting and increases possibilities for multiphonics. This study focusses on the first venting effect by staying in the chalumeau register and not using register changes
Data associated with 'Cryopreservation of primary cultures of mammalian somatic cells in 96-well plates benefits from control of ice nucleation'
No full textCryopreservation of mammalian cells has, to date, typically been conducted in cryovials, but there are applications where cryopreservation of primary cells in multiwell plates would be advantageous. However excessive supercooling in the small volumes of liquid in each well of the multiwell plates is inevitable without intervention and tends to result in high and variable cell mortality. We describe a technique for cryopreservation of adhered primary bovine granulosa cells in 96-well plates by controlled rate freezing using controlled nucleation. This dataset contains experimental data derived from granulosa cell culture cryopreservation experiments and measurements of ice nucleation temperature measurements observed within 96-well plates and other cryocontainer