1401 research outputs found
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Dataset associated with 'Quantification of Ebola virus replication kinetics in vitro'
No full textThis dataset is associated with ‘Quantification of Ebola virus replication kinetics in vitro’. In the paper, a mathematical model is developed to describe the replication of Ebola virus in Vero cells. The model is parametrised using measurements of total and infectious extracellular virus at three different multiplicities of infection. The results provide insights into the distribution of time an infected cell spends in the eclipse phase (the period between infection and the start of virus production), as well as the rate at which infectious virions lose infectivity. Here, we provide all the experimental results necessary to parametrise the mathematical model
Data associated with 'Looking beyond the core: The role of flanking regions in the aggregation of amyloidogenic peptides and proteins'
No full textRaw data for Figure 3 in the associated publicatio
Dataset associated with "Toward programmed complex mechanical deformations of Liquid Crystal Elastomers"
No full textUsing an empirical model encapsulating much of the anisotropic and nonlinear mechanical behaviour of Liquid Crystal Elastomers (LCEs), we study the localised deformation behaviour of a complexly-aligned LCE. This dataset provides the raw data from our tensile mechanical tests and the python scripts used to analyse photographs of the sample during mechanical deformation
Frequency of hand-to-head, -mouth, -eyes, and -nose contacts for adults and children during eating and non-eating macro-activities data
No full textHand to face contact frequencies
Hand to face contact sequences
R code for analysising data and creating figures published in the attached pape
Data associated with the Energies article 'Quantifying the Environmental Impacts of Cookstove Transitions: A Societal Exergy Analysis Based Model of Energy Consumption and Forest Stocks in Honduras'
No full textData and calculations for the societal exergy analysis and forest stock model for Honduras
See Sheet1 of HN_PSB_forecast_data_noIEA2.xlsx for Readm
Dataset for Macromolecular crowding enhances the detection of DNA and proteins by a solid-state nanopore.
No full textNanopore analysis of nucleic acid is now routine, but detection of proteins remains challenging. Here, we report the systematic characterization of the effect of macromolecular crowding on the detection sensitivity of a solid-state nanopore for circular and linearized DNA plasmids, globular proteins (beta-galactosidase), and filamentous proteins (alpha-synuclein amyloid fibrils). We observe a remarkable ca. 1000-fold increase in the molecule count for the globular protein beta-galactosidase and a 6-fold increase in peak amplitude for plasmid DNA under crowded conditions. We also demonstrate that macromolecular crowding facilitates the study of the topology of DNA plasmids and the characterization of amyloid fibril preparations with different length distributions. A remarkable feature of this method is its ease of use; it simply requires the addition of a macromolecular crowding agent to the electrolyte. We therefore envision that macromolecular crowding can be applied to many applications in the analysis of biomolecules by solid-state nanopores
Bio-Inspired Preparation of Clay-Hexacyanoferrate Composite Hydrogels as Super Adsorbents for Cs+ : dataset
No full textData associated with the paper Bio-Inspired Preparation of Clay–Hexacyanoferrate Composite Hydrogels as Super Adsorbents for Cs+, published in ACS Applied Materials & Interfaces
Data for ‘Resolving the size of ice-nucleating particles with a balloon deployable aerosol sampler: the SHARK’
No full textIce-nucleating particles (INPs) affect cloud development, lifetime and radiative properties, hence it is important to know the abundance of INPs throughout the atmosphere. A critical factor in determining the lifetime and transport of INPs is their size, however very little size-resolved atmospheric INP concentration information exists. This is especially so in the free troposphere. Here we present the development and application of a radio-controlled payload capable of collecting size-resolved aerosol from a tethered balloon for the primary purpose of offline INP analysis. This payload, known as the SHARK (Selective Height Aerosol Research Kit), consists of two complementary cascade impactors for aerosol size-segregation from 0.25 to 10 µm, with an after-filter and top stage to collect particles below and above this range at flow rates up to 100 L min-1. The SHARK also contains an optical particle counter to quantify aerosol size distribution between 0.38 and 10 µm, and a radiosonde for the measurement of temperature, pressure, GPS altitude, and relative humidity. This is all housed within a weatherproof box, can be run from batteries for up to 11 h and has a total weight of 9 kg. The radio control and live data link with the radiosonde allow the user to start and stop sampling depending on meteorological conditions and height, which can, for example, allow the user to avoid sampling in very humid or cloudy air, even when the SHARK is out of sight. While the collected aerosol could, in principle, be studied with an array of analytical techniques, this study demonstrates that the collected aerosol can be analysed with an off-line droplet freezing instrument to determine size-resolved INP concentrations, activated fractions and active site densities, producing similar results to those obtained using a standard PM10 aerosol sampler when summed over the appropriate size range. Test data is presented from four contrasting locations having very different size resolved INP spectra: Hyytiälä (Southern Finland), Leeds (Northern England), Longyearbyen (Svalbard), and Cardington (Southern England)
Bacterial transfer to fingertips during sequential surface contacts with and without gloves
No full textSingle Molecule Protein Stabilisation Translates to Macromolecular Mechanics of Protein Network - Dataset
No full textFolded globular proteins are attractive building blocks for biopolymer-based materials, as their mechanically resistant structures carry out diverse biological functionality. While much is now understood about the mechanical response of single folded proteins, a major challenge is to understand and predictably control how single protein mechanics translates to the collective response of a network of connected folded proteins. Here, by utilising the binding of maltose to hydrogels constructed from photo-chemically cross-linked maltose binding protein (MBP), we investigate the effects of protein stabilisation at the molecular level on the macroscopic mechanical and structural properties of a protein-based hydrogel. Rheological measurements show an enhancement in the mechanical strength and energy dissipation of MBP hydrogels in the presence of maltose. Circular dichroism spectroscopy and differential scanning calorimetry measurements show that MBP remains both folded and functional in situ. By coupling these mechanical measurements with mesoscopic structural information obtained by small angle scattering, we propose an occupation model in which higher proportions of stabilised, ligand occupied, protein building blocks translate their increased stability to the macroscopic properties of the hydrogel network. This provides powerful opportunities to exploit environmentally responsive folded protein based biomaterials for many broad applications