Scientific Journals of INIA (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria)
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Salicylic and citric acid as promising new stimulants for resin tapping in maritime pine (Pinus pinaster Ait.)
Get PDFAim of the study: We tested alternative active principles to the most widely used resin tapping stimulant which contains sulphuric acid. We also studied the effect of wounding in five-year-old Pinus pinaster seedlings with a microtapping method.Area of study: The experiment was carried out at the Universidad Politécnica de Madrid in Spain.Material and Methods: The experiment consisted of six treatments: control (no stimulant no wounding), wound (no stimulant), and pines stimulated with sulphuric acid, ethrel, salicylic acid and citric acid. We evaluated the resin yield differentiating between released resin and internal resin (resin retained within the xylem), and the physiological status of the tree.Main Results: Wounded plants produced on average three times more resin than control plants. Plants stimulated with salicylic and citric acids showed the highest resin yield and produced on average 15% more resin than those stimulated with sulphuric acid, mainly because the released resin was higher. Tree diameter affected resin yield and thicker trees produced more resin. We did not observe any significant effect of the treatments on stomatal conductance and only a marginal significant effect (p<0.10) on water potential.Research highlights: Salicylic acid and citric acid seem to be promising stimulants for the resin tapping activity to be further tested in field experiments with adult trees.Keywords: sulphuric acid; ethrel; pine resin; microtapping; wounding; water potential; stomatal conductance.Abbreviations used: TR: total resin content; RR: released resin; IR: internal resin; gs: stomatal conductance; Ψleaf: midday leaf water potential
Leaf litter hides post-dispersed seeds from invasive alien removers in a sclerophyll forest in central Chile
Get PDFAim of the study: Seed removal may be a determining filter for regeneration. Factors such as leaf litter, tree cover and seed density affect seed removal. This aims to assess three aspects involving on post dispersal seed removal of four woody species (Lithraea caustica, Maytenus boaria, Quillaja saponaria and Retanilla ephedra) of a sclerophyll forest.
Study area: Andean Mediterranean sclerophyll forest of Quillaja saponaria and Lithraea caustica located in the Río de Los Cipreses National Reserve, Chile.
Materials and methods: Seven experimental plots were set up, in which seeds were offered in an experiment with variations in leaf litter, tree cover and seed density. Generalized Linear Mixed Models (GLMM) were used for the statistical analysis. Camera traps were installed to identify the seed removers.
Main results: Q. saponaria seeds were more removed than the seeds of the other species (0.84, observed mean). Whereas that M. boaria and R. ephedra did not show significant differences on the removal of their seeds (0.77 and 0.67 respectively), both were more removed than L. caustica (0.59). The removal of M. boaria, Q. saponaria and R. ephedra seeds was lower in the presence of leaf litter. No factor influenced the removal of L. caustica seeds. Seed removers were identified as invasive alien species such as Rattus sp. and Oryctolagus cuniculus and native species such as Lycalopex culpaeus.
Research highlights: Three woody species of this forest suffer severe seed removal by invasive alien fauna with major implications for the resilience of these forests. The coverage of leaf litter is key to hide away the seeds, increasing survival and could promote germination.
Keywords: invasive alien fauna; Mediterranean forest; sclerophyll forest; seed removal factors
Characterization of the Spanish juniper population of El Peñón de Alamedilla (Granada, Spain): conservation status and protection proposal
Get PDFAims of the study: To characterize the southernmost Spanish Juniper (Juniperus thurifera L.) population in Europe, a relict species of the tertiary flora.Area of study: El Peñón de Alamedilla, Los Montes region (Granada, Spain).Material and Methods: A census and geo-referencing of the individuals in the population was carried out, together with the determination of the population structure, inference of the potential extension of the population, and discussion of the risk faced by the population.Main results: A total of 899 individuals were located in mainly 128 ha from 806 to 888 m a.s.l. We found 50.6% adults, 35.7% saplings and 13.6% juveniles. Sexing on the adults showed a 60% of male feet compared to 39.1% of females (0.9% undifferentiated). 47% of the population has a basal diameter less than or equal to 50 mm. A 74.11% of the population was in grasslands + scrubs, and only 5.3% of the specimens were in arable lands. All individuals are on privately owned land.Research highlights: The characterization of this southernmost Spanish Juniper population suggests a good viability and resilience, though there is also a high risk of inbreeding, with the population being able to go into genetic drift, which is critical for successful reproduction and may easily jeopardize its conservation efforts. This species is not protected enough in Andalusia and conservation measures should be taken.Keywords: Juniperus thurifera, SE Iberian Peninsula, relict population, Natura 2000 Network
Production efficiency of different crop rotations and tillage systems
Get PDFAim of study: The production efficiency of crop rotations was evaluated based on the yield of the main crop (cereal grains and pea seeds) and yield of the by-product (straw) converted per cereal units (CU), and on total protein yield.
Area of study: South-eastern Poland, Europe (2017-2019).
Material and methods: The first order experimental factor included crop rotations: A): peas–durum wheat–spring barley; B): spring wheat–durum wheat–spring barley +oats; and C): spring barley–durum wheat–spring wheat. The second order experimental factor included tillage systems: CT, conventional tillage, RT, reduced tillage, and NT, no-tillage.
Main results: The yield of pea seeds, cereal grains, and straw per CU was higher in crop rotation A than B (127.8 CU vs. 101.1 CU). Higher CU yields were also recorded in crop rotation C than B (by 18.9 CU). The tillage system had no effect on CU yield. The total protein yield was significantly higher in crop rotation A (2110.7 kg ha-1) than in crop rotations B (by 808.8 kg ha-1) and C (by 448.0 kg ha-1). A higher protein yield was also recorded in RT than in the NT system.
Research highlights: The units used for CR assessment, i.e. CU and total protein yield, enable to reliably evaluate the production yield of both CRs and tillage systems
Drought responsiveness in two Mexican conifer species forming young stands at high elevations
Get PDFAim of study: To determine the response of high-altitudinal forests to seasonal drought.Area of study: Monte Tláloc, Estado de México and Rancho Joyas del Durazno, Municipality of Río Verde, San Luis Potosí, México.Materials and methods: In this study, we evaluate the response to drought and hydroclimate in two young Mexican conifers sampled at high elevation, correlating records of tree-ring growth and the Normalized Difference Vegetation Index (NDVI).Main results: The results show that Pinus teocote and Abies religiosa are vulnerable to the precipitation regime and warm conditions of winter-spring. The physiological response mechanisms seem to be differentiated between the species, according to the effects of drought stress. The NDVI demonstrated the different temporal responses of the species according to their inherent physiological mechanisms in response to hydroclimatic limitations. This differentiation can be attributed to the spatial variation present in the particular physical and geographic conditions of each area. The dry and warm seasonal climates reveal P. teocote and A. religiosa to be species that are vulnerable to drought conditions. However, further evaluation of the resistance and resilience of these species is necessary, as well as disentanglement of the effects of associated mechanisms that can influence the predicted processes of extinction or migration.Research highlights: Pinus teocote and Abies religiosa are vulnerable to the seasonal drought conditions. These results are of particular importance given the climatic scenarios predicted for elevated ecotones. Tree-ring widths and NDVI improved the response of radial growth to the climate, enhancing our understanding of forest growth dynamics. The response to climatic variability depends on the particular species.Keywords: High elevation; tree-ring; ENSO; NDVI; climate-growth relationship. Abbreviations used: Normalized Difference Vegetation Index (NDVI); Tree-Ring Width (TRw); precipitation (PP); maximum temperature (Tmax); minimum temperature (Tmin); El Niño-Southern Oscillation (ENSO); Climatic Research Unit Time-series data version 4.04 data (CRU TS v. 4.04); Standardized Precipitation-Evapotranspiration Index (SPEI); Climatic Research Unit Time-series data version 4.03 data (CRU TS v. 4.03); first-order autocorrelation (AC); mean sensitivity (MS); mean correlation between trees (Rbt); expressed population signal (EPS); Ring Width Index (RWI)
Short communication: Virulence of Puccinia triticina in the North Caucasus region of Russia
Get PDFAim of study: To analyze the structure of P. triticina populations by a virulence survey in the North Caucasus region of Russia from 2011 to 2015.Area of study: The North Caucasus region is a leading grain production region in Russia where wheat leaf rust causes losses in yield.Material and methods: Uredinial samples of leaf rust were collected in all agro-climatic zones of the North Caucasus on the production sites of winter wheat and on the plots of official state trials. Single uredinial isolates (a total of 564) were tested for virulence with 41 ˈThatcherˈ near isogenic lines with Lr resistance genes.Main results: Clones virulent to Lr9, Lr42, Lr47 and Lr50 were not found. Isolates virulent to the Lr19, Lr24, Lr29, Lr41, Lr43 + 24, Lr45, Lr52 genes were characterized by low frequencies. The 564 fungal isolates studied were represented by 564 virulence phenotypes, the majority of them with a virulence complexity from 9 to 19. A high level of intrapopulation fungus diversity in virulence was noticed during the whole period of research (Shannon diversity index from 2.994 to 3.314). The differences in the frequencies of virulences in the years of research were small (Rogers distance from 0.001 to 0.160).Research highlights: Due to the fact that the North Caucasus region is a zone of epiphytotic danger and high variability of the P. triticina population, the analysis of the genetics of the fungus population is important for the strategy of varietal distribution in the region and development of rust-resistant varieties
Technical-economic viability of mechanized picking coffee (Coffea arabica L.) in up to three annual operations
Get PDFAim of study: Unavailability, coupled with the burden of labor for agricultural services nowadays, has made the mechanization process of harvesting of fallen coffee (Coffea arabica L.) essential. Although this operation has essential importance, it is often not monitored and executed in search of extreme quality. Considering the search for higher profits, this study aimed to analyze the performance of a coffee picker in three passes in an area in order to collect and process all the material and its economic viability.Area of study: The experiment was carried out in July 2017 in the Brazilian Cerrado, in the municipality of Presidente Olegário, Minas Gerais, Brazil, at Fazenda Gaúcha/Café.Material and methods: The amount of gathered coffee was equivalent to 600 kg ha−1 of processed coffee. The data from 2017 were used to analyze the economic viability of the picking operation. Treatments were distributed in split-blocks with three passes of the picking machine. The analyzed variables were picking and cleaning efficiency, picking losses, and percentage of vegetal and mineral impurities.Main results: Coffee losses reached the minimum level in the third pass. However, the harvesting operation could be carried out at most twice in the same area from the economic point of view under the evaluated conditions.Research highlights: Mechanized picking of coffee can be performed at most twice in the same area, providing a positive economic return
Comparison of the technical and economic performances of two different shredders on pomegranate pruning residues
Get PDFAim of study: The study aimed to examine the technical and economic performances of two different shredders for three windrow densities of pomegranate residues.Area of study: The study was conducted in the Serik District of Antalya Province, Turkey.Material and methods: Two different pruning residue shredders driven by tractor power take off (PTO) were used. Machine‑I has pick‑up, shredding, screen units. Machine‑II only has a shredding unit. The experiment was conducted at windrow densities of 1.49, 2.10, and 2.41 kg/m2 in a pomegranate orchard; the study used a completely randomized split‐plot design with two treatments and three replications.Main results: The power values for the increasing windrow densities were 8.00, 11.73, and 18.47 kW/m for Machine-I and 5.08, 5.68, and 6.48 kW/m for Machine-II. Moreover, the average particle length of 68.6 mm shredded by Machine‑I was approximately 20 mm smaller than that of Machine‑II. The minimum unit energy value of Machine-II was 2.53 kWh/t at the maximum windrow density of 2.41 kg/m2. This value for Machine-I was 5.58 kWh/t at the medium windrow density of 2.10 kg/m2. The lowest unit cost for Machine-I and Machine-II was calculated as 27.2-7.1 US/t (at maximum density), respectively.Research highlights: The appropriate windrow densities for Machine-I and Machine-II were different in terms of energy requirements and total unit cost. Machine-I is more effective at consistently chopping the residues than Machine-II, but it requires more energy and a higher unit cost
Short Communication: Laboratory assessment of ammonia volatilization from pig slurries applied on intact soil cores from till and no-till plots
Get PDFAim of study: Agricultural activities are the main source of volatilized ammonia (NH3). Maximum rates are reached within a few hours after slurry application. This study aimed to evaluate the influence of soil texture, tillage and slurry dry matter (DM) on NH3 volatilization.Area of study: Mediterranean semiarid environments (NE Spain).Material and methods: Ammonia volatilization from pig slurry directly applied on the soil surface was quantified in the laboratory, in soil samples from two experimental sites with different soil textures: silty loam and sandy loam. Field treatments consisted of two tillage management practices: till by disc-harrowing or no-till. At topdressing (cereal tillering), tillage treatments were combined with slurries of different DM contents applied onto the silty loam soil. Measurements were done for two cereal cropping seasons and during the period of maximum NH3 flux (12 h after slurry application). A photoacoustic analyzer was used.Main results: Slurry spreading at sowing resulted in low volatilization (0.7-9% of NH4+-N applied) as it also did at topdressing (0.3-1.4% of NH4+-N applied). At sowing, ammonia volatilization from high DM slurry (>7.5%) was significantly enhanced by no-till in both soils. At topdressing, this result was also found in records on silty loam soil. No differences were found between tillage systems when slurry of low DM content was applied, whatever the soil texture and application moment. Although NH3 volatilization was probably affected by the laboratory conditions, the comparisons between treatments were still valuable.Research highlights: Ammonia volatilization abatement can be improved (<1 kg NH3-N ha-1) if fertilization is done after crop establishment using low DM slurries (<3.5%)
Effect of antimicrobial peptides and monoterpenes on control of fire blight
Get PDFAim of study: Antimicrobial peptides and monoterpenes are safe compounds that have been used for control of many plant diseases. Herein, the effects of two recombinant antibacterial peptides (AMPs) were compared with two monoterpenes for control of Erwinia amylovora directly or via induction of plant defense enzyme guaiacol peroxidase (GPOD).Area of study: The experiments were performed at the Ferdowsi University of Mashhad (Iran).Material and methods: The central composite design (CCD) method was used to study the effect of mixing the compounds and copper compound (Nordox) in controlling the pathogen. The resistance level was studied on shoots of tolerant (‘Dargazi’) and semi-susceptible (‘Spadona’) pear cultivars treated with the antibacterial compounds.Main results: Thanatin and 1,8-cineole showed the highest and lowest antibacterial effects. All treatments reduced E. amylovora pathogenicity on blossom. The CCD analysis revealed that the best reduction in colony number obtained by mixing Lfc, thanatin, thymol, 1,8-cineole and Nordox at concentrations of 32, 16, 24, 250 and 250 μg/mL. Thymol and 1,8-cineole at 500 μg/mL decreased disease severity significantly compared to that of AMPs. The level of GPOD enzyme in ‘Dargazi’ was higher than in ‘Spadona’. All treatments increased the GPOD levels in both cultivars. Furthermore, resistance level and GPOD ratio were negatively correlated.Research highlights: Antimicrobial peptides showed better effect on growth inhibition of E. amylovora than monoterpenes. Mixing of these peptides and monoterpens at special dosage enhanced their antimicrobial efficacy against E. amylovora; that could represent a new method in control of fire blight disease