Scientific Journals of INIA (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria)
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Is GNSS real-time positioning a reliable option to validate erosion studies at olive grove environments?
Aim of study: Soil degradation in agricultural areas is a widespread problem. In this framework, a data validation methodology is presented, including a study of the spatial resolution of Global Navigation Satellite System (GNSS) measurements, the calculation of erosion/deposition models, and the contribution of dual frequency and low-cost single frequency GNSS receivers.Area of study: A test olive grove in SE Spain.Material and methods: The study is based on three observation campaigns, between 2016 and 2018, using different GNSS receivers and working modes. The comparison between different surveys provide the volumetric variation over the analyzed period.Main results: Considering the dual-frequency receiver, there was no statistically significant difference between the means and the variances from 1.5 m and from 4.5 m data resolution at the 0.05 significance level. In order to estimate vertical differences from successive GNSS campaigns a differential digital elevation approach was applied. Although the differences depended on the zone of the test area and they changed along the monitoring period, the erosion rate could be catalogued as very low. The dual-frequency receiver satisfied the vertical centimetric precision limits for high accurate Digital Elevation Model (DEM), making it a reliable and accurate option to validate erosion studies in small areas.Research highlights: The results have allowed the characterization of multi-annual spatial redistribution of the topsoil at local scale, being of great help to design future prevention actions for the “tillage erosion” in olive grove environments. However, more tests are needed to guarantee the feasibility of low-cost receivers
Direct heat stress-induced effects on rumen fermentation characteristics and nutrients degradability in sheep pair-fed alfalfa hay
Aim of the study: To investigate the direct effect of heat stress on rumen fermentation characteristics and nutrients degradability of pair-fed rams to subsequently eliminate the confounding effects of dissimilar feed intake induced by heat stress exposure.Area of study: Saudi Arabia.Material and methods: Five rumen-cannulated desert rams (45 ± 1.63 kg body weight; 2–3 years of age) were placed individually in controlled climatic-chambers to be exposed to two successive periods. The 1st period was a control thermoneutral period (TN; 23.64 ± 0.14 °C; extended for 21 days) followed by a 2nd period of heat stress (HS; 44.26 ± 1.70 °C, for another 21 days). Each period was consisted of a temperature acclimation phase (7 days) and a data collection phase (14 days). Alfalfa hay was offered twice daily during both periods in a pair-fed manner (800 g DM per head and day).Main results: Exposing pair-fed desert rams to elevated ambient temperature had (p<0.05) elevated their respiration rate and skin temperature, without noticeable (p>0.05) changes in their rectal temperature. Most of the rumen fermentation characteristics and nutrients degradability were not affected by HS (p>0.05). However, exposure to HS increased (p<0.05) pre-feeding rumen total volatile fatty acids concentrations, pre-feeding molar proportion of acetate, and post-feeding rumen osmolality.Research highlights: HS had no direct effect on post-feeding rumen fermentation characteristics and nutrients degradability in desert sheep. This implies that strategic approaches to mitigate the adverse effects of HS have to be directed towards promoting feed intake and nutrients utilization under such conditions
Influence of production system and finishing feeding on meat quality of Rubia Gallega calves
Aim of study: Beef quality is mainly affected by finishing feeding (FF) and production system (PS). The effects of PS (extensive, semi-extensive, traditional and intensive systems) and FF from Rubia Gallega calves were compared in terms of meat quality.Area of study: Galicia (NW Spain)Material and methods: Calves (n=10 per treatment) were slaughtered at nine months of age and meat samples were assessed in terms of meat quality attributes such as physicochemical, nutritional and sensory analysis performed by a trained panel.Main results: Meat chemical composition varied (p<0.01) with PS and FF. The semi-extensive system showed the lowest values (p<0.05) for cooking loss (22.8%) and for shear force (26.0 N) while the extensive system presented the lowest contents of saturated fatty acids (SFA) and the highest contents of linolenic acid (p<0.001). The SFA and polyunsaturated fatty acid (PUFA) contents varied with FF (p<0.05). The lowest and highest values for SFA and PUFA were displayed in extensive group finished with Pasture/Concentrate, meanwhile feedlot group finished with Concentrate/Straw showed and opposite trend. The n-6/n-3 ratio was lower than 4 for extensive and semi-extensive systems (p<0.001). In addition, tenderness and juiciness showed significant differences mainly due to FF.Research highlights: This study showed that meat of calves reared in extensive systems was the healthiest, regarding total fat and fatty acid composition. It can be concluded that finishing feeding, and PS affected meat quality. Hence the potential usefulness of these results is to improve meat quality and safety according to market demands
Multi-criteria analysis to compare multiple risks associated with management alternatives in planted forests
Aim of study: Adaptation of silviculture in planted forest may help to mitigate damage due to biotic and abiotic hazards. However, compromises have to be found because it is not possible to minimize the risk from all hazards through application of a single forest management approach. The objective of this study was to improve a multi-criteria risk analysis (MCRA) method that makes it possible to rank forest management alternatives (FMAs) according to multiple risks. Material and Methods: We defined eight FMAs for maritime pine forests in France, Spain and Portugal. We used as the definition of risk the combination of hazard, susceptibility and exposure. Hazard level was estimated using archive data on occurrence and severity of damaging agents over the last few decades. Forest susceptibility to hazards was evaluated by experts who scored the effect on stand resistance of eleven silvicultural operations characterizing each FMA. Exposure was estimated as value at stake, which combined forest standing volume, simulated with forest growth models, and wood prices.Main Results: Using the PROMETHEE algorithm, we found that the overall ranking of FMAs was consistent across all countries, with short rotation plantations to produce pulpwood or energy wood were the least at risk. The ranking was mainly driven by forest values at stake. We found that by improving the accuracy of forest values exposed to damage, based on growth models and representative wood prices, the MCRA outcomes were more useful and realistic.Research highlights: Our methodology provides a relevant framework to design FMAs that would minimize risks while maintaining income.Keywords: Pinus pinaster; vulnerability; hazards; growth modelling; expert assessment; wood price; southwestern Europe
Optimum cork stopper diameter for a proper wine sealing performance when modifying bottleneck diameter: a first approach
Aim of study: This study present a theoretical model that allow establishing the proper relationship between forces and diameters that take part in sealing for ensuring an adequate closure during storage time, and obtained the optimum stopper diameter for a proper sealing performance when modifying bottleneck diameter.Area of study: The proposed model is of interested to the whole cork value chain from forest owners to natural cork stoppers manufacturers.Material and methods: The optimum cork stopper diameter depends mainly on stopper quality and the compression rate applied in the bottling operation. In this study, we establish the stopper diameter when reducing bottleneck diameter, applying a compression rate of 33% when corking, and for natural cork stoppers which quality allows to recover its initial diameter to 96% after 24 h since compression.Main results: For a bottleneck diameter of 18 mm, the value of the stopper diameter should be at least of 22.3 mm, and for a bottleneck diameter of 17 mm, the value of the stopper diameter should be at least of 20.3 mm.Research highlights: These results try to solve one of the main worries of natural cork stopper manufacturers, which is the scarcity of raw cork suitable for manufacturing them. However this study is also of interested to forest owners because the increment of cork suitable for natural cork stoppers manufacturing means an increment in cork value.Key words: bottling; corking; compression force; compression rate; diameter recovery; relaxation force; relaxation ratio.Abbreviations used: Ds (Cork Stopper Diameter); Dg (Caliper Diameter the Corking Machine); Db (Bottleneck Diameter); Dr (Recovered Diameter); Fc (Compression Force); Fr (Relaxation Force); CR (Compression Rate); RR (Relaxation Ratio); RD (Diameter Recovery)
Artificial intelligence with deep learning algorithms to model relationships between total tree height and diameter at breast height
Aim of Study: As an innovative prediction technique, Artificial Intelligence technique based on a Deep Learning Algorithm (DLA) with various numbers of neurons and hidden layer alternatives were trained and evaluated to predict the relationships between total tree height (TTH) and diameter at breast height (DBH) with nonlinear least squared (NLS) regression models and nonlinear mixed effect (NLME) regression models.Area of Study: The data of this study were measured from even-aged, pure Turkish Pine (Pinus brutia Ten.) stands in the Kestel Forests located in the Bursa region of northwestern Turkey.Material and Methods: 1132 pairs of TTH-DBH measurements from 132 sample plots were used for modeling relationships between TTH, DBH, and stand attributes such as dominant height (Ho) and diameter (Do).Main Results: The combination of 100 # neurons and 8 # hidden layer in DLA resulted in the best predictive total height prediction values with Average Absolute Error (0.4188), max. Average Absolute Error (3.7598), Root Mean Squared Error (0.6942), Root Mean Squared error % (5.2164), Akaike Information Criteria (-345.4465), Bayesian Information Criterion (-330.836), the average Bias (0.0288) and the average Bias % (0.2166), and fitting abilities with r (0.9842) and Fit Index (0.9684). Also, the results of equivalence tests showed that the DLA technique successfully predicted the TTH in the validation dataset.Research highlights: These superior fitting scores coupled with the validation results in TTH predictions suggested that deep learning network models should be considered an alternative to the traditional nonlinear regression techniques and should be given importance as an innovative prediction technique.Keywords: Prediction; artificial intelligence; deep learning algorithms; number of neurons; hidden layer alternatives.Abbreviations: TTH (total tree height), DBH (diameter at breast height), OLS (ordinary least squares), NLME (nonlinear mixed effect), AIT (Artificial Intelligence Techniques), ANN (Artificial Neural Network), DLA (Deep Learning Algorithm), GPU (Graphical Processing Units), NLS (nonlinear least squared), RMSE (root mean squared error), AIC (Akaike information criteria), BIC (Bayesian information criterion), FI (fit index), AAE (average absolute error), BLUP (best linear unbiased predictor), TOST (two one-sided test method).
Short communication: Antimicrobial activity of indoleacetic, gibberellic and coumaric acids against Paenibacillus larvae and its toxicity against Apis mellifera
Aim of study: To explore three isolated phytomolecules: indoleacetic acid (IAA), gibberellic acid (GA), and the secondary metabolite p-coumaric acid (CUM): (1) evaluating their toxicity against Apis mellifera larvae and adults under controlled conditions in the laboratory; (2) searching for antimicrobial activity against Paenibacillus larvae.Area of study: Honey bee larvae and adults were collected from the experimental apiary of the “Centro de Investigación en Abejas Sociales (CIAS)” (-37.9348798, -57.682817), Institute of the National University of Mar del Plata (UNMdP), Argentina.Material and methods: Paenibacillus larvae strains were isolated from beehives from different provinces of Argentina (Buenos Aires, Córdoba and Entre Ríos) showing clinical symptoms of the American foulbrood. All strains (S1, S2, S3, S4) were genotypically identified using PL5 and PL4 primers and characterized as genotype ERIC1. Then standard essays were performed to determined toxicity of phytomolecules in honey bees and antimicrobial activity through the broth microdilution method.Main results: The diet with GA, IAA and CUM did not present toxic effects in larvae or adult bees, and only CUM showed antimicrobial activity against P. larvae. In this study, we obtained in vitro values of MNIC (minimum non-inhibitory concentration) of 500 μg mL-1 and a MIC (minimum inhibitory concentration) of 650 μg mL-1 for CUM.Research highlights: The obtained results remark its potential as a natural alternative for the control of P. larvae, avoiding the problems generated by the use of synthetic antibiotics such as the resistance phenomena and the contamination of hive’s products
Soil nitrogen sequestration in a long-term fertilizer experiment in central China
Aim of study: To evaluate the effects of a long-term manuring and fertilization experiment on the soil total N concentration and its storage and sequestration rates in the rice-wheat cropping system.Area of study: A rice-wheat rotation area in central China.Material and methods: A 35-yr long-term fertilizer experiment was conducted with 9 treatments: unfertilized (Control), N, P, and K fertilizers, manure (M) and M combined with N, P, and K fertilizers treatments. Soil total N input amount, total N concentration, total N storage amount and N sequestration rate in soil were calculated.Main results: The soil total N input amount, N concentration, N storage amount and N sequestration rate were significantly influenced by M and chemical fertilizers. In total, 0.017-0.021 g N/kg soil accumulated in the organic M plots, whereas only 0.005-0.007 g in chemical fertilizer alone plots. The highest soil total N storage amount was 6.09 t/hain the M alone plot, and the lowest value was 4.46 tN/ha in the N fertilizer alone plot. The highest N sequestration rate in soil was 0.061 t N/ha/yr in the high amount M plus NPK fertilizers plot, and the lowest value was 0.002 tN/ha/yr in the N fertilizer alone plot. A significant nonlinear regression relationship existed between the total N sequestration rate in soil and annual total N input amount. Moreover, the average soil total N concentration was significantly positively correlated with the average grain yield of crop and soil organic C concentration. The soil total N sequestration rate in M alone or M combined with inorganic fertilizer treatments were increased compared with inorganic fertilizer alone treatments.Research highlights: Considering crop yields and total N sequestration rate in soil, the use of manure combined with inorganic fertilizer should be recommended in the rice-wheat cropping system
Acute stress assessment using infrared thermography in fattening rabbits reacting to handling under winter and summer conditions
Aim of study: This study assesses acute stress by measuring, through infrared thermography in summer and winter, the temperature of the eye, outer ear, inner ear and nose in 40 fattening rabbits before and after handling.Area of study: Seville (Spain).Material and methods: Body thermographic temperatures were recorded during a 38-day fattening period twice weekly and twice a day, before and after the handler held the rabbits in their arms for one minute. Ambient temperature and relative humidity were also recorded, and their influence on body temperatures was assessed. For each anatomical part, the variation of the temperature between the handled and undisturbed rabbit, and the differential temperature between the anatomical part in the undisturbed rabbit and the ambient temperature were calculated.Main results: The variation in temperatures between handled and undisturbed rabbits ranged from 0.25±0.041 ºC for eye to 3.09±0.221 ºC for outer ear in summer and -0.41±0.182 ºC for nose to 2.09±0.178 ºC for outer ear in winter. The day of the fattening period influenced all the temperature traits during summer and winter, except for the inner ear in winter. In summer, unlike winter, the temperature variation at the end of fattening period between handled and undisturbed rabbits was lower than at weaning (-0.04 to 1.94 ºC vs. 0.54 to 5.52 ºC, respectively). The temperatures in undisturbed rabbits were correlated with ambient temperature.Research highlights: Measuring body temperature with infrared thermography is a useful tool to evaluate acute stress in handled rabbits, with the inner ear and eye the most reliable body parts for measuring it
Nanomaterials. Effective tools for field and horticultural crops to cope with drought stress: A review
Drought is the most serious environmental challenge that limits plant growth and causes more severe yield losses than other abiotic stress factors resulting in a serious food shortage. Nanomaterials (NMs) are considered as vital tools to overcome contemporary and future challenges in agricultural production. Recently, NMs have been applied for enhancing seed germination, growth, physiology, productivity and quality attributes of various crops under normal or stress conditions. Up to date, there is no a comprehensive review about the potential role of NMs in attenuating the drought-induced adverse effects in crop plants. Thus, this review will highlight this issue. Generally, NMs minimize drought-induced osmotic stress by accumulation of osmolytes that result in osmotic adjustment and improved plant water status. In addition, NMs play a key role to improve root growth, conductive tissue elements and aquaporin proteins facilitating uptake and translocation of water and nutrients. Furthermore, NMs reduce water loss by stomatal closure due to abscisic acid signaling. However, this leads to reduced photosynthesis and oxidative stress damage. At the same time, NMs increase the content of light-harvesting pigments, enzymatic and non-enzymatic antioxidants leading to enhancing photosynthesis with reducing oxidative stress damage. Overall, NMs can ameliorate the deleterious effects of drought stress in crop plants by regulation of gene expression and alternation of various physiological and biochemical processes