Scientific Journals of INIA (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria)
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Designing acorn protection for direct seeding of quercus species in high predation areas
Aim of the study: a) To present the trial-and-error approach followed in the design and patent of a manufactured seed and seedling protector effective against mice and voles, rabbits, wild boar and deer (http://bopiweb.com/elemento/829172/). b) To assess the viability of direct oak seeding with and without protection in the complex acorn predation reality of post fire restoration and underplanting in existing pine afforestations.Study area: Northern Plateau of Spain, in an area of extreme acorn predation.Material and methods: We followed a classical trial-and-error approach for problem solving. Different modifications to a wire mesh screen cylinder were tested in subsequent trials aiming to evaluate the effects on acorn predation and early emerging plant survival and growth. The final protector is based on a thin wire mesh cylinder with three innovations: a truncated cone, a circular crown and a sphere compartment. Further we assessed the viability of direct oak seeding with and without protection in the complex acorn predation reality of post fire restoration and underplanting in existing pine afforestations.Main results: The manufactured seed protector was found to be effective against synergic attacks of mice, rodents, wild boars and herbivores. Survival of protected oak was 77% under canopies and 32% in open light conditions two years after sowing.Research highlights: Our results confirm the viability of direct oak seeding for woodland restoration if seed predation is controlled. Keywords: sowing; underplanting; trial-and-error; seed protector; afforestation; oak
Current and future estimates for the fire frequency and the fire rotation period in the main woodland types of peninsular Spain: a case-study approach
Aim of study. Fire regimes are frequently dynamic and change as a function of the interactions between the three main fire drivers: fuels, ignitions and climatic conditions. We characterized the recent period (1974-2005) and performed estimates for the future fire regimeArea of study. We have considered five pine and another four woodland types by means of the analyses of 100 reference areas in peninsular Spain.Material and methods. The estimates of the expected alterations in fire frequency and the fire rotation period were based on models previously developed for the climatic scenarios SRES A2 and B2.Main results. The results point to the large variability in fire frequency and rotation periods between the woodland types as defined, and also among the reference areas delimited for each of them. Fire frequencies will increase for all woodland types while very relevant shortenings of the fire rotation periods are expected. For the 32 yr period analysed, rotation periods longer than 500 yr were obtained in 54% of the reference areas while this percentage would decrease to 31% in the B2 and to 29% in the A2 climatic scenario. In the most affected woodland type, P. pinaster, from a median rotation period of 83 yr it would decrease to 26 yr in the B2 and to 20 yr in the A2 climatic scenario.Research highlights. We conclude that the predicted increases in fire activity will have adverse effects on some of the main Spanish woodland types due to the expected future disruptions in the fire regime. Keywords: Forest fires; fire regime; fire frequency; fire rotation period; climatic change.Abbreviations used: SRES: Special Report on Emissions Scenarios; IPCC: Intergovernmental Panel on Climate Change; RA: Reference Areas
Influence of anatomy and basic density on specific cutting force for wood from Corymbia citriodora Hill & Johnson
Aim of the study: The aim of this study was to evaluate the influence of xylem tissue cell structure, determined through biometry and basic density of the wood from Corymbia citriodora Hill & Johnson on consumption of specific 90º-0º longitudinal cutting force.Area of study: The study area was in the region of the Vale do Rio Doce - Minas Gerais, Brazil.Material and methods: A diametrical board with dimensions of 60 x 18 x 5 cm (length x width x thickness, respectively), with more than 1.3 m from the ground, was removed. In machining trials, a 400 mm diameter circular saw was used, with 24 “WZ” teeth, feed rate of 10 m.min-1, cutting speed of 61 m.s-1, and maximum instantaneous torque of 92.5 N.m. During cutting, test specimens were removed with alternated and parallel 1.5 cm edges in 6 radial positions, which were used for biometric determination of cell structure and basic density.Main results: It was observed that wood basic density, vessel diameter, fiber wall thickness, fiber wall fraction and fiber wall portion were directly proportional to the specific cutting force. In contrast, vessel frequency and fiber lumen diameter proved to be inversely proportional to cutting force.Research highlights: This work provides important values of quantification of influence of xylem tissue cell structure, determined through biometry and physical properties of the wood that may be used to prediction of consumption of specific cutting force.Keywords: wood machining; wood properties; optimization of the process
Predicting methionine and lysine contents in soybean meal and fish meal using a group method of data handling-type neural network
Artificial neural network models offer an alternative to linear regression analysis for predicting the amino acid content of feeds from their chemical composition. A group method of data handling-type neural network (GMDH-type NN), with an evolutionary method of genetic algorithm, was used to predict methionine (Met) and lysine (Lys) contents of soybean meal (SBM) and fish meal (FM) from their proximate analyses (i.e. crude protein, crude fat, crude fibre, ash and moisture). A data set with 119 data lines for Met and 116 lines for Lys was used to develop GMDH-type NN models with two hidden layers. The data lines were divided into two groups to produce training and validation sets. The data sets were imported into the GEvoM software for training the networks. The predictive capability of the constructed models was evaluated by their abilities to estimate the validation data sets accurately. A quantitative examination of goodness of fit for the predictive models was made using a number of precision, concordance and bias statistics. The statistical performance of the models developed revealed close agreement between observed and predicted Met and Lys contents for SBM and FM. The results of this study clearly illustrate the validity of GMDH-type NN models to estimate accurately the amino acid content of poultry feed ingredients from their chemical composition
Selection of a suitable model for the prediction of soil water content in north of Iran
Multiple Linear Regression (MLR), Artificial Neural Network (ANN) and Rosetta model were employed to develop pedotransfers functions (PTFs) for soil moisture prediction using available soil properties for northern soils of Iran. The Rosetta model is based on ANN works in a hierarchical approach to predict water retention curves. For this purpose, 240 soil samples were selected from the south of Guilan province, Gilevan region, northern Iran. The data set was divided into two subsets for calibration and testing of the models. The general performance of PTFs was evaluated using coefficient of determination (R2), root mean square error (RMSE) and mean biased error between the observed and predicted values. Results showed that ANN with two hidden layers, Tan-sigmoid and linear functions for hidden and output layers respectively, performed better than the others in predicting soil moisture. In the other hand, ANN can model non-linear functions and showed to perform better than MLR. After ANN, MLR had better accuracy than Rosetta. The developed PTFs resulted in more accurate estimation at matric potentials of 100, 300, 500, 1000, 1500 kPa. Whereas, Rosetta model resulted in slightly better estimation than derived PTFs at matric potentials of 33 kPa. This research can provide the scientific basis for the study of soil hydraulic properties and be helpful for the estimation of soil water retention in other places with similar conditions, too
Antifungal potential of Bacillus vallismortis R2 against different phytopathogenic fungi
The cash crops grown in an agro-climatic region are prone to infection by various fungal pathogens. The use of chemical fungicides over the years has resulted in emergence of resistant fungal strains, thereby necessitating the development of effective and environmental friendly alternatives. The natural antagonistic interactions among different microbial populations have been exploited as an eco-friendly approach for controlling fungal pathogens resistant to synthetic chemicals. Morphologically distinct bacterial cultures (150), isolated from rhizospheric soils of wheat, rice, onion and tomato plants were screened for their antifungal potential against seven phytopathogenic fungi prevalent in the State of Punjab (India). The bacterial isolate R2, identified as Bacillus vallismortis, supported more than 50% inhibition of different phytopathogenic fungi (Alternaria alternata, Rhizoctonia oryzae, Fusarium oxysporum, Fusarium moniliforme, Colletotrichum sp, Helminthosporium sp and Magnaporthe grisea) in dual culture plate assay. The thin layer chromatography based bio-autography of acid-precipitated biomolecules (APB) indicated the presence of more than one type of antifungal molecule, as evidenced from zones of inhibition against the respective fungal pathogen. The initial analytical studies indicated the presence of surfactin, iturin A and fengycin-like compounds in APB. The antifungal activity of whole cells and APB of isolate R2 was evaluated by light and scanning electron microscopy. The wheat grains treated with APB and exposed to spores of A. alternata showed resistance to the development of black point disease, thereby indicating the potential application of R2 and its biomolecules at field scale level
Phenology and interspecific association of Forficula auricularia and Forficula pubescens in apple orchards
The European earwig Forficula auricularia L. (Dermaptera: Forficulidae) has been widely studied as a key predator of pests in temperate regions, but its phenology and behavior may differ in warmer areas such as the Mediterranean. Here we assessed the phenology, aggregation, and interspecific association of F. auricularia and Forficula pubescens Gené, the only two species found consistently in both ground and canopy shelters in Mediterranean apple orchards. In addition to F. auricularia and F. pubescens, three other earwig species, namely Labidura riparia Pallas, Nala lividipes Dufour and Euborellia moesta Gené, were found occasionally. The mature stages of F. auricularia were observed mainly from May to November in tree shelters and immature ones from October to June in ground shelters. Adult individuals of F. pubescens were observed year-round and nymph instars were detected from April to June in ground as well as in tree shelters. The suitability of the current degree-days models for temperate regions was evaluated for the prediction of European earwig phenology in a Mediterranean climate. Regarding interspecific association, F. auricularia and F. pubescens co-occurred in canopies without apparent competition. This study provides useful weekly data about the phenology of the two earwig species throughout the year that can be used to detect the key periods during which to enhance their populations in pip fruit orchards or to control them in stone fruit crops. Furthermore, our results are of relevance for the development of new phenological models of earwigs in Mediterranean areas where nymphs hibernate, a feature that makes current models inaccurate
The effects of three techniques that change the wetting patterns over subsurface drip-irrigated potatoes
Wetting pattern enhancement is one of the goals of irrigation designers and researchers. In this study, we addressed three techniques (dual-lateral drip, intermittent flow and physical barrier methods) that change the wetting pattern of subsurface drip irrigation. To study their effect on the yield and water-use efficiency (WUE) of potatoes, field experiments were conducted for four seasons, during which the soil-water balance was continuously monitored using a set of capacitance probes. The results of the soil water patterns showed that both the dual-lateral and intermittent techniques increased lateral water movement and eliminated deep percolation, whereas the physical barrier had a limited effect on the top soil layer. The crop results indicated that the yield and WUE increased significantly in response to the application of the dual-lateral drip (up to 30%); the intermittent application also positively affected the yield (~10%) and the WUE (~14%), but these effects were not statistically significant according to the statistical model. The physical barrier showed a non-significant negative effect on the yield and WUE. These findings suggest the following recommended practices: the use of dual-lateral drip technique due to its beneficial results and its potential for increasing yields and reducing water consumption; the application of intermittent flow with more than three surges; and restricting the use of physical barriers to soils with high permeability
Short Communication. Embryo yield in llamas synchronized with two different intravaginal progesterone-releasing devices and superovulated with eCG
The objectives of this study were to compare the effects of two intravaginal devices (ID) containing the same dose (0.5 g) of progesterone (P4) on subsequent ovarian response, embryo production and circulating P4 concentration profile in llamas (Lama glama) treated with equine chorionic gonadotropin (eCG) for ovarian superstimulation. Female llamas were randomly assigned (n = 10 llamas per group) to one of the following groups and treated (Day 0) with an ID containing 0.5 g of vegetal P4 to synchronize the emergence of a new follicular wave: i) DIB 0.5® and ii) Cronipres M15®. On Day 3 llamas were intramuscularly treated with 1000 IU of eCG. The IDs were removed on Day 7. Llamas were naturally mated (Day 9) and treated with GnRH analogue to induce ovulation. A second mating was allowed 24 h later. Embryos were collected between 7 and 8 days after the first mating. Blood samples were taken every day from Day 0 to Day 7 to measure circulating P4 concentrations. The results indicated that DIB device maintained greater plasma P4 levels as compared to Cronipres until Day 2. However, the mean (± SD) number of corpora lutea and recovered embryos was not affected (p < 0.05) by the type of ID (5.3 ± 2.6 vs 4.2 ± 2.2 and 3.5 ± 2.7 vs 2.6 ± 3.0 for DIB and Cronipres, respectively). In conclusion, both DIB and Cronipres devices can be successfully used to synchronize the emergence of follicular wave prior to a single dose of eCG in superovulation protocol in llamas
Effects of feeding dry glycerol on milk production, nutrients digestibility and blood components in primiparous Holstein dairy cows during the early postpartum period
The aim of this study was to evaluate the glucogenic property of glycerol supplementation in the dairy cow’s diet. Sixty primiparous cows (control, n=30, and glycerol supplemented, n=30) were used to measure milk yield and components, blood hormone and metabolite profiles, and body condition score. Feed intake and apparent total-tract digestibility were also measured using 10 primiparous cows (control, n=5, and glycerol supplemented, n=5). Dry glycerol was top dressed at 250 g/day/cow from parturition to 21 days postpartum. Average feed intake, milk yield and components were not affected by glycerol supplementation. Apparent total–tract digestibility of organic matter and neutral detergent fibre were not influenced by dry glycerol supplementation, but lipid digestibility was greater (p=0.01) in cows fed glycerol. The serum concentration of glucose and insulin tended to be higher in dry glycerol-supplemented cows (p=0.1; p=0.06, respectively). While, serum concentrations of nonesterified fatty acids and β-hydroxybutyrate were not affected. Supplemented cows had lower body condition loss during weeks 1 to 5 after calving (p=0.09). The glucogenic effect of glycerol did not affect milk yield during the first 3 weeks of lactation. However, daily milk yield during the 13 weeks recording period was higher in the glycerol-supplemented cows (28.5 vs. 30.3 kg, p<0.001). Percentages of cows cycling at the planned breeding date was greater (p=0.01) for cows fed dry glycerol. The results demonstrated that feeding dry glycerol as a glucogenic supply could be useful in saving body reserves and improving energy balance of primiparous Holstein dairy cows during the early postpartum period