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Testing the links between income levels and Subjective Well-Being: The case of the Greek regions
No full textIn recent years, the debate on conceptualization of development has been reawakened triggering significant discussions both in academic, as well as policy making circles. One of the main causes lies in the growing recognition of GDP shortcomings as a suitable indicator of social welfare
Έκθεση διάχυσης αποτελεσμάτων υλοποίησης Δράσεων 1-5 [Υποέργο: Το ανθρώπινο δυναμικό ως βασική συνιστώσα της αναπτυξιακής δυναμικής και διαφοροποίησης των περιοχών: Η περίπτωση της Ελλάδας]
No full textΠρωταρχικό Υλικό µελέτης (Πρώτες ύλες) για την µεθοδική έρευνα σχετικά µε την πρωτεϊνική έκφραση βιο-υµενικών κυττάρων (Π5-Δ1.1 & Δ1.2)
Get PDFΟ πειραματικός σχεδιασμός στοχεύει στη δημιουργία πρωταρχικού υλικού για τη μελέτη της συνολικής γονιδιωματικής έκφρασης σε βιο-υμένια τροφικών παθογόνων: γονιδιωματικές, μεταγραφωματικές, πρωτεομικές και μεταβολομικές προσεγγίσεις (genomics transcriptomics proteomics, metabolomics)
Morphological, Physiological and Metabolomic Response of transgenic tobacco plants (N. tabacum L.) overexpressing GmGSTU4 under Drought Stress
Get PDFGSTs appear to have a significant role in plants’ adaptation under abiotic stress as many isoenzymes are found to be differentially expressed under these conditions yet, little is known about the regulatory functions of GSTs. Wild type and transgenic tobacco plants over-expressing the soybean GmGSTU4 of cultivars Basmas, Burley and Virginia were grown in vitro under 100 and 200mM mannitol or in soil (plant pots) by withholding watering for 15 days. However, GmGSTU4 plants did not exhibit significant
differences in drought tolerance compared to wild-type plants. Morphological (shoot length, total and root fresh weight) and physiological (chlorophyll content, relative water content and photosynthetic capacity) parameters of transgenic plants did not differ from the wild-type in the presence of 100 or 200mM mannitol or in the soil when watering was halted. Metabolite profiling was used to understand the dynamics between the wild-type and transgenic tobacco response to drought stress. Different metabolic pathways are involved in production of osmoprotectants. These molecules accumulate in plants under stress conditions as adaptive mechanism, which can provide stress tolerance. GmGSTU4 plants did not exhibit difference in drought tolerance compared to wild-type plants, however metabolomics analysis indicated alterations in metabolite profile and increased concentration of sorbitol, glycerol and pyruvic acid. In conclusion, overexpression of GmGSTU4 in transgenic plants did not
affect their drought stress tolerance although it has altered their metabolite profile possible because of diverse effects on plant stress tolerance mechanism
Over-expression of a specific soybean GmGSTU4 isoenzyme improves chloroacetanilide herbicide tolerance of transgenic tobacco plants
Get PDFPlant glutathione transferases (GSTs) have a major role in herbicide detoxification. Soybean (Glycine max L.) GmGSTs have been well studied for their correlation in herbicides selectivity towards diphenyl ether, chloroacetanilide and sulfonylurea herbicides. Chloroacetanilide herbicide tolerance was assayed in vitro by measuring the growth inhibition of wild type (wt) and transgenic tobacco seedlings from cultivars (Basmas, Virginia, Burley) in the presence of 7.5 and 15 mg/L of alachlor and metolachlor. Alachlor caused strong inhibition of shoot and root growth of wt tobacco plants. All the transgenic Basmas lines showed significantly higher shoot and root elongation at 7.5 mg/L alachlor, with line BAGST-3 exhibiting the greatest tolerance. However, at 15 mg/L alachlor, growth was highly reduced in transgenic and wt plants. In Burley, only line BUGST-2 has statistically significant greater mean of root and shoot length compared to wt under the two doses. On the contrary, Virginia has reduced growth which was similar to the wt. Metolachlor toxicity was less severe compared to alachlor. Growth of the transgenic lines of the three cultivars was not significantly greater in either
metolachlor concentrations tested, compared to wt plants, except line BAGST-3 which exhibited significantly greater mean of shoot and root elongation at 7.5 mg/L. Transgene expression was determined quantitatively using Real Time qPCR, lines BAGST-3 and BUGST-2 showed greater expression of Gmgstu4 in shoot compared to root. These results confirm that overexpression of GmGSTU4 in tobacco provides higher catalytic activities towards xenobiotics, resulting for future use in environmental cleanup of alachlor
MDR-involved human glutathione transferases (hGSTs) are targets for inhibition by 2,2'-dihydroxybenzophenones and N-carbonyl analogues
Get PDFOver expression of human GSTA1-1 in tumour cells is part of MDR mechanisms. Substituted 2-hydroxybenzophenones are ubiquitous in naturally occurring and synthetic compounds, exhibiting important biological activities. 2,2’-Dihydroxybenzophenones and N-carbonyl analogues, structurally, are ringopened forms of xanthone analogues which we reported recently as hGSTA1-1 inhibitors. The present study combined GST inhibition screening, in silico molecular docking and enzyme inhibition kinetics, revealing four analogues with strong inhibitory potency (IC50 = 0.18-1.8 μM) and modest cytotoxic activity for Caco2 cell line (LC50 = 35 to > 400 μM), thus being useful as lead structures for the design of new inhibitors against hGSTs
Τεχνική αναφορά εργασίας υπαίθρου στην Πελοπόννησο
Get PDFΗ εργασία υπαίθρου που εκπονήθηκε κατά τις ημέρες 30/06/2013 έως 02/07/2013 είχε ως στόχο την μελέτη των μεταλπικών αποθέσεων (ηλικίας κατωτέρου Πλειοκαίνου) Νότιο-ανατολικά της χερσονήσου της Μεσσηνίας
Λειτουργικός χαρακτηρισμός του ισοενζύμου PVGSTU2-2 (p. vulgaris) στην μεταβολική αποδόμηση του ζιζανιοκτόνου dimethenamid
Get PDFΟι τρανσφεράσες της γλουταθειόνης, έχουν κεντρικό ρόλο στο μηχανισμό μεταβολικής αποδόμησης ζιζανιοκτόνων των φυτικών οργανισμών. Το γονίδιο PvGSTU2-2 απομονώθηκε από τα φύλλα του είδους Phaseolus vulgaris και το ισοένζυμο που κωδικοποιεί καταλύει την συμπλοκοποίηση της γλουταθειόνης με τα ζιζανιοκτόνα alachlor και metalachlor της ομάδας των χλωροακεταμιδίων. Προκειμένου να μελετηθεί in planta η ικανότητα του ισοενζύμου PvGSTU2-2 να μεταβολίζει ζιζανιοκτόνα της ομάδας αυτής, δημιουργήθηκαν με την μέθοδο του αγροβακτηρίου διαγονιδιακά φυτά καπνού που υπερεκφράζουν το γονίδιο PvGSTU2-2. Η παρουσία του διαγονιδίου επιβεβαιώθηκε με PCR πραγματικού χρόνου (RTPCR) και η έκφραση του με ποσοτική PCR. Περεταίρω επιβεβαιώθηκε πως το διαγονίδιο κωδικοποιεί ενεργές πρωτεΐνες με την μέτρηση της ειδικής ενζυμικης δραστικότητας στο υπόστρωμα NBD-CI. Επιλέχθηκαν τρεις διαγονιδιακές σειρές οι οποίες αξιολογήθηκαν ως προς την ανθεκτικότητα τους στο ζιζανιοκτόνο dimethenamid (0,5 and 1 mg/L). Κάτω απο in vivo συνθήκες οι διαγονιδιακές σειρές επέδειξαν μεγαλύτερη ανθεκτικότητα και στις δύο συγκεντρώσεις καθώς παρουσίασαν μεγαλύτερο μήκος ρίζας και βλαστού σε σχέση με τα φυτά μάρτυρες. Μέσω της προσέγγισης αυτής, επιβεβαιώθηκε ο ρόλος του ισοενζύμου PvGSTU2-2 στην εκλεκτικότητα τοωροακεταμιδίων
Synthesis of glutathione analogues and screening as substrates & inhibitors for human glutathione transferase p1‐1
Get PDFA major detoxification mechanism of the cell involves the glutathione transferase (GST)‐catalyzed formation of glutathione (GSH) conjugates with various xenobiotics Based on the same mechanism, GST overexpression may lead to multidrug resistant phenotypes Therefore, several compounds with inhibitory potency against GSTs have been developed as potential tools fortackling GST-‐attributed MDR. Several individual compounds and prodrugs have been proposed as GST‐inhibiting substances. In addition, GSH analogues have been considered as specific GST inhibitors, with particular attention been directed towards the synthesis of GSH analogues stable against γ‐glutamyltranspeptidase (γGT) and peptidases, as GST inhibitors