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Π7.1 Παροχή υπηρεσιών επιτόπιας τεχνικής υποστήριξης και πρακτικής εκπαίδευσης σε σχολεία Α’ βάθμιας και Β’ βάθμιας, εξαμηνιαίες αναφορές
Discovering probiotic microorganisms: invitro, invivo, genetic and omics approaches
Over the past decades the food industry has been revolutionized toward the production of functional foods due to an increasing awareness of the consumers on the positive role of food in wellbeing and health. By definition probiotic foods must contain live microorganisms in adequate amounts so as to be beneficial for the consumer’s health. There are numerous probiotic foods marketed today and many probiotic strains are commercially available. However, the question that arises is how to determine the real probiotic potential of microorganisms. This is becoming increasingly important, as even a superficial search of the relevant literature reveals that the number of proclaimed probiotics is growing fast. While the vast majority of probiotic microorganisms are food-related or commensal bacteria that are often regarded as safe, probiotics from other sources are increasingly being reported raising possible regulatory and safety issues. Potential probiotics are selected after in vitro or in vivo assays by evaluating simple traits such as resistance to the acidic conditions of the stomach or bile resistance, or by assessing their impact on complicated host functions such as immune development, metabolic function or gut–brain interaction. While final human clinical trials are considered mandatory for communicating health benefits, rather few strains with positive studies have been able to convince legal authorities with these health claims. Consequently, concern has been raised about the validity of the workflows currently used to characterize probiotics. In this review we will present an overview of the most common assays employed in screening for probiotics, highlighting the potential strengths and limitations of these approaches. Furthermore, we will focus on how the advent of omics technologies has reshaped our understanding of the biology of probiotics, allowing the exploration of novel routes for screening and studying such microorganisms
Μελέτες Περίπτωσης για το Τμήμα Τουρκικών και Σύγχρονων Ασιατικών Σπουδών
Αναλύονται 5 επιχειρηματικά σχέδια: δύο Ε.Π.Ε. παροχής υπηρεσιών εστίασης, ένα διαδικτυακό φροντιστήριο Ανατολίτικων Σπουδών, μία ομόρρυθμη επιχείρηση εμπορίου παραδοσιακών ανατολίτικων τροφίμων και ποτών και μία πράσινη ξενοδοχειακή μονάδα σε τουρκικό έδαφος, στη Σμύρνη
Μελέτη του σχηματισμού βιοϋμενίων στο νοσοκομειακό παθογόνο και προσφάτως συγκαταλεγόμενο ως παράγοντα διατροφικού κινδύνου Staphylococcus aureus subsp. aureus COL (MRSA)
Τα στελέχη του Staphylococcus aureus MRSA-τύπου (methicillin resistant) είναι στην συντριπτική τους πλειοψηφία απομονωμένα από κλινικές (HA – hospital-associated) και δευτερευόντως από εξάρσεις κρουσμάτων στην ευρύτερη πολιτεία (CA –communityassociated) ή τη ζωική παραγωγή (LA – livestock-associated). Είναι ανθεκτικά στη μεθικιλλίνη κι άλλες β-λακτάμες και ιδιαίτερα δύσκολο να καταπολεμηθούν. Προκαλούν από απλές δερματικές παθήσεις μέχρι θανατηφόρες συστημικές λοιμώξεις, κι αποτελούν σοβαρό κίνδυνο για ασθενείς με ανοιχτές πληγές, ιατρικά πρόσθετα ή αδύναμο ανοσοποιητικό.
The vast majority of Staphylococcus aureus MRSA (methicillin-resistant) strains are primarily isolated from clinics (HA – hospital-associated) and to a lesser extend from the wider population (CA – community-associated) or animal farms (LA – livestock-associated). They are resistant to β-lactam antibiotics including methicillin and extremely difficult to treat. They cause from mild skin disorders to serious systemic infections, and pose as deadly threat to patients with open wounds, medical implants or compromised immune system
Sol-gel immobilization of glutathione transferase: efficient tool for bioremediation
Glutathione transferases are multi-functional enzymes with an important role in xenobiotic detoxification. They catalyse the nucleophilic addition of the sulfur atom of glutathione (γ-L-Glu-L-Cys-Gly, GSH) to the electrophilic groups of a large variety of hydrophobic molecules including organic halides, epoxides, arene oxides, α- and β-unsaturated carbonyls, organic nitrate esters, and organic thiocyanates. The conjugation of GSH to such molecules increases their solubility and reduces their toxicity. GSTs represent a versatile tool with a variety of biotechnological applications, in the field of bioremediation to clean up environmentally contaminated sites. The purpose of this project was the study of GST
immobilization for the biodegradation of toxic compounds
Interactions encountered inside dual-species biofilms formed by Salmonella Typhimurium and autochthonous microbiota recovered from leafy salads on stainless steel
In the present study, the ability of bacteria isolated from leafy salads to affect biofilm formation by Salmonella Typhimurium (ST), when all these were cultured together on stainless steel (SS) coupons, was investigated. To achieve this, isolates recovered from either rocket or spinach salads were left to form mixed culture dual-species biofilms with ST on SS coupons immerged in: (i) LB medium, (ii) rocket sterile extract, and (iii) spinach sterile extract, at 20°C