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EHD1-positive endocytic PM-recycling vesicles contribute to cell plate formation and root development in arabidopsis
No full textRecycling of internalized endocytic proteins to the plasma membrane (PM) occurs at the trans-Golgi network (TGN) in plant cells where the secretory and endocytic protein trafficking pathways converge. Little is known about the nature and identity of PM-recycling vesicles in plants. A family of proteins containing the Epidermal growth factor receptor substrate 15-Homology Domain (EHD) are markers of recycling, and EHD bearing tubules function in slow-recycling of internalized proteins from sorting endosome to the PM in animal cells. To define the PM-recycling pathway in plants, we cloned the Arabidopsis homolog of mammalian EHD1 and generated a green fluorescent protein (GFP)-fusion and expressed it in tobacco BY-2 cells and Arabidopsis plants. Cytosolic punctate structures labeled by GFP-EHD1 fusion or endogenous EHD1 are distinct from the Golgi apparatus and prevacuolar compartment (PVC), but partially colocalize with a TGN marker. EHD1-positive structures do not lie on the endocytic pathway as judged by FM4-64 labelling. Pharmacological studies revealed that Brefeldin A, but neither Concanamycin A nor Wortmannin, inhibits EHD1 PM-recycling. During interphase, EHD1 and a Rab11 homolog RabA1g partially colocalize as cytosolic punctae, but highlight the developing cell plate during meiosis. Using an inducible RNA interference (RNAi) approach, we showed that EHD1 knockdown by RNAi could hamper root development and cell plate formation in Arabidopsis
Drop by drop
Get PDFIn October 2013 Yale University professor Sheila Levrant de Bretteville began exploring the water\u27s edge at four Hong Kong harbours with an interdisciplinary group of students from HKDI. The issues that captured their attention formed the basis of some vivid and emotionally compelling work
End game
Get PDF2012 has long been the subject of myths and speculation because it appeared to be the year in which the world was supposed to be destroyed, according to some scholars of the Mayan calendar. So far this year has not seen a global cataclysm but, as Summer Cao reports, it has inspired a hit movie and a popular HKDI show
Picture this
Get PDFHong Kong photographers Leong Ka Tai and Tse Ming Chong discuss the skills required to create an image that is both elegant and powerful
A fitting tribute
Get PDFWe explore the history of the qipao, and how this iconic dress has come to represent so muc
Outstanding alumni
Get PDFOrganised by the Hong Kong Design Centre, the Hong Kong Young Design Talent Awards celebrate and support up-and-coming designers, awarding sponsorships for overseas placements to help them reach their potential. Eunice Tsang speaks with four HKDI graduates among this year\u27s winners
Master strokes
Get PDFA new exhibition at HKDI will reveal how one of China\u27s most famous contemporary artists created a unique synthesis between East and Wes
Fit to be tied
Get PDFIn conjunction with the 8th International Shibori Symposium, HKDI presented The Animal Fiber: Art Informs Shibori from 13 December 2011 to 14 January 2012, revealing how an ancient technique has grown into an art form revered by couturiers and a medium of experimentation loved by children of all ages
Fresh inspiration 「心」鮮有道
Get PDFHélène Darroze was recently named as Veuve Cliquot\u27s World\u27s Best Female Chef for 2015. She comes from four generations of chefs and despite a brief attempt to work outside the kitchen she is now a global culinary star. From her Michelin-starred restaurant in London she told Daniel Jeffreys how she manages two world-class kitchens, a family of two young girls and still finds time to mentor young culinary professionals.
Hélène Darroze最近被法國凱歌香檳(Veuve Cliquot)評選為2015世界最佳女廚師。Darroze來自烹飪世家，祖上三代均為廚師，雖然她曾短暫離開廚房以外環境工作，但回歸廚房後已搖身成為全球頂尖的廚藝明星。在她位於倫敦的米芝蓮星級餐廳，她告訴我如何管理兩間世界一流的餐廳，盡心教養一對女兒之餘，還能抽空向不少年輕廚師傳授經驗