Bioscientia Medicina - Journal of Biomedicine and Translational Research
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The Effectiveness of Turmeric Extract (Curcuma domestica Val.) Against Fibroblast Cells in Socket Post Tooth Extraction
Get PDFBackground. This study is an exploration to assess the potential of turmeric extract in the induction of fibroblast cell proliferation, where fibroblast cells are important cells in the process of repair and healing of wounds (socket wounds after tooth extraction). The antioxidants found in turmeric extract are believed to have potential in the initiation of growth factors which will then initiate the process of fibroblast cell activation, which in turn will play a role in wound healing.
Methods. The research design in this study is an experimental study with a post-test-only approach with a control group design. The research subjects in this study were rats (Rattus norvegicus) Wistar strain, male sex, age 8-12 weeks, and body weight between 150-250 grams. A total of 30 white rats were grouped into 5 groups. Data analysis was carried out with the help of SPSS 25 software. First, a univariate analysis was performed to present the mean number of fibroblast cells and the standard deviation. Furthermore, an analysis was carried out to assess the difference in the mean number of fibroblast cells between groups using the one-way ANOVA test.
Results. The results of the one-way ANOVA test showed that there were significant differences between the test groups. Giving 10% and 15% turmeric extract was seen to be more potent in the initiation of the number of fibroblast cells than the treatment group that received povidone-iodine.
Conclusion. Turmeric extract (Curcuma domestica Val.) concentration of 15% was the most effective concentration as an effort to increase the number of fibroblast cells after tooth extraction in Wistar rats
Arrhythmia Mechanism on Diabetes Mellitus: A Narrative Review
Get PDFDiabetes mellitus is a metabolic disorder characterized by an increase in blood sugar levels. The mechanism of arrhythmias in patients with diabetes mellitus is complex and unique. This is an independent factor in the occurrence of arrhythmias through several slightly different mechanisms in non-diabetics. Hyperglycemia, hypoglycemia, remodeling, autonomic neuropathic dysfunction, fibrosis, and oxidative changes, and metabolic stress play an important role in the occurrence of arrhythmias in diabetes. This narrative review was at least to describe the mechanism of arrhythmia in diabetes mellitus
Cortisol Level Related to Depression in Women with Endometriosis
Get PDFBackground: Several main symptoms of endometriosis can lead to physical and psychological stress and also hormonal disturbances as the result of prolonged stress. Cortisol was associated with the onset of depression. This study aimed to determine the relationship between cortisol levels and depression in women with endometriosis at the Department of Obstetrics and Gynecology at Dr. Mohammad Hoesin General Hospital (RSMH) Palembang.
Methods: A cross-sectional study was performed at the Reproductive Endocrinology and Infertility outpatient clinic and inpatient ward, Department of Obstetrics and Gynecology at Dr. Mohammad Hoesin General Hospital Palembang, from January to November 2020. There were 74 samples of endometriosis patients who met the inclusion criteria. The blood cortisol level was examined using the ELISA method. The determination of depression level was performed by filling out the Beck Depression Inventory questionnaire. Data were analyzed using SPSS version 22.0.
Results: Seventy-four samples met the inclusion criteria. As many as 44 patients (59.5%) without depression, 20 patients (27%) with mild depression, 8 patients (10.8%) with moderate depression, and only 2 patients (2.7%) with severe depression. There were no differences in age, marital status, parity, menstrual cycle, and duration of treatment between endometriosis patients with and without depression (p > 0.05). There was a significant difference in cortisol levels between endometriosis patients with and without depression (p = 0.017). Endometriosis patients with cortisol levels ≤ 7.4 mg/dl were three times more at risk of depression than endometriosis patients with cortisol levels > 7.4 mg/dl. In addition, there was a significant relationship between cortisol level and depression status (OR = 3.023 (95% CI 1.153–7.942; p = 0.041). This study also found a significantly low negative correlation between cortisol levels and BDI scores (r = -0.249; p = 0.032).
Conclusion: There was a significant relationship between serum cortisol levels and depression in women with endometriosis. Endometriosis patients with depression had significantly lower cortisol levels than endometriosis patients without depression
Gaucher Disease with Normocytic Normochrome Anemia Manifestation in a 13-Month-Old Baby: A Case Report
Get PDFBackground: Gaucher disease is a sphingolipidosis, an inherited disorder of metabolism resulting from glucocerebrosidase deficiency, causing the deposition of glucocerebroside and related compounds. This study aimed to describe Gaucher disease in one year old baby girl.
Case presentation: A 13-month-old girl was referred from the district hospital with a diagnosis of hemolytic anemia. The Patient come with major complaints in the form of enlarged abdomen since 6 months ago. The patient looked pale and coughed since 7 days ago, accompanied by a cold. Ultrasound examination of the abdomen shows splenomegaly. Based on supporting examinations, the effect of normocytic normochrome anemia with reticulocytosis was obtained. The bone survey resulted in an Erlenmeyer flask image on both the humerus and femur suitable for Gaucher disease. The results of microscopic evaluation on bone marrow smear found large cells with Gaucher cell characteristics. Laboratory evaluation of glucocerebroside enzymes showed that there was a deficiency deficiency β-glucosidase. Patients get enzyme replacement therapy for 7 times, each therapy is carried out every 2 weeks.
Conclusion: Enzyme replacement therapy is the definitive effective therapy in patients with Gaucher disease. Follow-up should, in principle, be individualized, as the heterogeneity of the disease and a number of associated conditions precludes strict protocolized follow-up
Diagnosis and Treatment-Spectrum Beta-Lactamase and Multidrug Resistance Bacterial
Get PDFMultidrug-resistant (MDR) is a condition resistant to at least one type of antibiotic from 3 classes of antibiotics. Extended-Spectrum Beta Lactamases are globular proteins that consist of alpha-helices and beta-pleated sheets. β-lactamase hydrolyze broad-spectrum cephalosporin with oxyimino side chain. ESBL hydrolyze antibiotics group penicillin, cephalosporin first, second, third, fourth generation, and monobactam aztreonam. Multidrug resistance occurs through two mechanisms, bacteria accumulate multiple genes encoding resistance to one antibiotic, and due to increased expression of genes encoding multidrug effluent pump, enzymatic inactivation and target structure change. Multidrug-resistant (MDR) caused by extended-spectrum resistance beta-lactamase (ESBL) can be detected by phenotyping and genotyping methods. Treatment for MDR ESBLs other than carbapenems can be β-lactam/β-lactamase inhibitor combinations (BLBLIs), namely piperacillin/tazobactam (PZT)
The Role of Galectin-3 in Acute Myocardial Infarction: A Narrative Literature Review
Get PDFGalectin-3 (Gal-3) is a protein, namely a lectin, that binds to beta galactosides. Gal-3 has been shown to be related to the process of fibrosis, classification of atherosclerosis, and cardiac remodeling. Gal-3 regulation is known to be involved in the process of atherogenesis. Low-density lipoprotein (LDL) Oxidation increases Gal-3 expression in vascular smooth muscle cells. Gal-3 also induces fibroblasts and vascular smooth muscle cells to proliferate and produce fibrosis-related proteins in the extracellular matrix. Inhibition of Gal-3 can reduce the atherosclerotic process and reduce the progression of plaque formation. Gal-3 also induces fibroblasts and vascular smooth muscle cells to proliferate and produce fibrosis-related proteins in the extracellular matrix. Inhibition of Gal-3 can reduce the atherosclerotic process and reduce the progression of plaque formation. Several clinical trials have focused on the role of Gal-3 in acute myocardial infarction patients showing that Gal-3 is upregulated. In these patients. This literature review aims to describe the role of Gal-3 in acute myocardial infarction
The Effect of Acemannan Hydrogel on Collagen Expression in Gingival Tissue of Diabetes Mellitus Animal Model
Get PDFBackground: Diabetes mellitus is a comorbid disease that greatly interferes with the process of wound healing, including injuries to the gingival tissue. The Aloe vera plant, or Aloe vera, is one of Indonesia's natural wealth, which is often found and has been widely used by the community in overcoming various health problems, including to treat various wounds. This study aims to explore the potential of hydrogen acemannan to repair gingival tissue in rats with diabetes mellitus.
Methods: An in vivo experimental study using 24 Wistar rats grouped into 4 groups (negative control, 25%, 50%, and 75% acemannan hydrogel). Collagen density was assessed histologically semiquantitatively. Data analysis was performed using SPSS using univariate, bivariate, and multivariate methods.
Results: The administration of acemannan hydrogel showed an increase in the collagen density score along with an increase in the concentration of acemannan hydrogel. Administration of 25%, 50%, and 75% acemannan hydrogel showed significant differences in the collagen density score compared to the group that did not receive acemannan hydrogel treatment, p<0.05.
Conclusion: Acemannan hydrogel application can increase collagen density in gingival tissue of diabetes mellitus animal model
Diagnosis and Management of Mirizzi Syndrome Related to Gallstones: A Narrative Literature Review
Get PDFMirizzi syndrome (MS) is a rare condition caused by compression of the common hepatic duct due to gallstones located in the cystic duct or neck of the gallbladder. Biliary pain, jaundice, and fever are the most common clinical manifestations of MS. Patients usually report a history of biliary pain with jaundice. There are no pathognomonic features in the history and physical examination. Patients may also come with acute cholecystitis, acute cholangitis, or pancreatitis. In cases where MS is suspected, the diagnosis can be made by ultrasonography (US), computed tomography (CT), magnetic resonance cholangiopancreatography (MRCP), and endoscopic retrograde cholangiopancreatography (ERCP). Surgery is the main treatment for cases of MS and is a challenge because preoperative diagnosis is difficult, thus impacting management during surgery. This literature review aimed to describe the features of MS related to gallstones and management options
The Role of the Managerial Function of Supervisor Model 4S towards Patient Safety Culture at Sawahlunto General Hospital
Get PDFBackground: The culture of patient safety in hospitals is still not well implemented. Maintaining a patient safety culture is the responsibility of every healthcare professional. This study aims to explore the relationship between the supervisory function of the 4S model and the culture of patient safety at Sawahlunto General Hospital.
Methods: This study is an observational study. A total of 52 respondents participated in this study. This study uses a questionnaire instrument to assess the role of supervisory and managerial functions in the 4S model with patient safety culture. Data analysis was performed by SEM-PLS.
Results: There is a significant relationship between the characteristics of nurses and patient safety culture at Sawahlunto General Hospital. There is a significant relationship between the characteristics of supervisors and interpersonal supervisors with the managerial function of supervisors at Sawahlunto General Hospital.
Conclusion: The managerial function of the Model 4S supervisor is related to the patient safety culture at Sawahlunto General Hospital
Strangulated Umbilical Hernia: A Case Report
Get PDFBackground: The abdominal wall hernia repair was one of the most frequently performed operations by general surgeons worldwide. The second most common hernia operation is repairs of umbilical hernia. It is estimated that 5% of individuals will develop an abdominal wall hernia over their lifetime. This study aimed to present a case of strangulated umbilical hernia.
Case presentation: 51 years old male patient presented with sharp, non-radiating pain in the left abdominal region, obstipation, and noted bulge at his umbilicus about one year ago that did not shrink when he was lying down two days ago, the diameter about 7 cm. A plain Abdominal X-ray showed partial small bowel obstruction. The patient was taken to the operation theatre for laparotomy resection and reanastomose ileum and repair hernia defect with mesh. Prior to surgery, the patient received antibiotic therapy with Cefazolin 2gr intravenous, and the nasogastric decompression tube (NGT) was installed. On exploration, it was found necrotic ileum along 15 cm and performed resection, followed by end-to-end anastomosis. After the surgical procedure, the patient's hemodynamics was stable.
Conclusion: Overall mortality and prognosis of hernia are influenced by whether the surgery was emergent or elective. Mortality is increased to up to 3% with emergency surgery for strangulated bowel, notably when bowel resection is performed