5576 research outputs found
Sort by
Mrub_3018 is Orthologous to \u3cem\u3eE. coli\u3c/em\u3e B2759 (casB)
Get PDFThis project is part of the Meiothermus ruber genome analysis project, which uses a collection of online bioinformatics tools to predict gene function. We studied the biological activity of the Mrub_3018 gene, which we hypothesize is orthologous to E. coli gene B2759. We predicted that Mrub_3018(DNA coordinates 3057916… 3058524) encodes the protein CasB. CasB is a protein in the CRISPR CASCADE that will function as a structural protein. When the rest of the proteins form an “S” formation CasB will connect the front and back of the “S” creating a back bone for the structure. It will help bind DNA while other proteins like Cas3 degrade the protein. The hypothesis was proven by identical hits in KEGG, COG, Pfam, TIGRfam, and PDB, as well as similar location(Cytoplasm) found using PSORT-B, PRED and TMHMM. The genes were also found to be part of an operon as they were flanked by genes that are in the CRISPR system
Mrub_3015 is orthologous to the b2757 gene found in \u3cem\u3eEscherichia coli\u3c/em\u3e coding for casD
Get PDFThis project is part of the Meiothermus ruber genome analysis project, which uses a collection of online bioinformatics tools to predict gene function. We investigated the biological function of the gene Mrub_3015, which we hypothesize is a component of the CRISPR-Cas prokaryotic defense system. We predict that Mrub_3015 (DNA coordinates 3055550...3056245) encodes the the CRISPR-associated protein cas5, which is integral in maintaining the crRNA-DNA structure, keeping the complex from base pairing with the target phage DNA. Our hypothesis is supported by identical hits for Mrub_3015 and b2527 to the KEGG, Pfam, TIGRfam, CDD and PDB databases as well as a low E-value for a pairwise NCBI BLAST comparison. Both protein products are predicted to be localized in the cytoplasm. Finally, both proteins share numerous highly conserved amino acids, when compared to the consensus sequence of Pfam domains PF09704, PF18019, and PF00270
Children That Never Grew Wrinkles
No full textThis piece of art comes in the digital form, capturing the disheartening reality of children in the Holocaust. Millions of young children lost their lives before they could even start them. As I reflect on events of The Holocaust, I am constantly reminded of how fortunate I am to be alive today and grow old. Never Forget
And Lead Me Not Into Temptation
Get PDFThis piece discusses my personal struggles with my weight as an plus-size woman by integrating the Lord\u27s Prayer throughout the piece in a creatively bittersweet manner. Body image distortions continue to be all too common for countless young girls and women as they struggle with them, but also the growing prevalence of body shaming in our society
\u3cstrong\u3eRooted and Open : The Common Calling of the Network of ELCA Colleges and Universities\u3c/strong\u3e
Get PDF
Effects of Heat Treatment of Spinels as Determined by VIS/NIR and Raman Spectroscopy and XRD
Get PDFHeat treatment is done to improve the color and clarity and, therefore, the value of gemstones. The objectives of this study include observing color and inclusion changes in pink spinels after heat treatment, and analyzing structural changes within differently colored spinels using Raman Spectroscopy and X-ray diffraction with a Paris Edinburgh Press. We expected the heat treatment to yield color changes and fading of small inclusions. Cut and polished spinels were heated to 1400C for 2 days or 850C for 3 days. Future experiments involve 800C, 750C, and 700C all for 1 week, 1500C for 4 days, and 1600C for 12 hours. To determine changes, Vis/NIR spectroscopy data were taken before and after heating. Only non-heat treated spinels were analyzed with the Paris Edinburgh Press; these XRD data show that the spinels with different colors are structurally different from one another. Raman spectroscopy was used in this study to observe structural changes in spinels that were exposed to heat. Six spinels were heated to 850C for 24 hours, six spinels were heated at 1200C for 6 hours, and the last six were heated at 1600C for 6 hours. The Raman spectroscopy data show that the six spinels exposed to 850C for 24 hours did not change structurally, but the 12 spinels exposed to 1200C or 1600C for six hours did change structurally