Jurnal Fakultas Farmasi Umi (Universitas Muslim Indonesia)
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ANALISIS KADAR FENOLIK FRAKSI ETIL ASETAT DAUN PETAI CINA (Leucaena leucocephala) (Lam.) de Wit) SECARA SPEKTROFOTOMETRI Uv-Vis
Get PDFTraditionally, river tamarind leaves (Leucaena leucocephala (Lam.) De Wit.) can be used as medicine for injuries, swollen, diarrhea and also as antiseptic. The river tamarind leaves contains several main compounds such as alkaloids, saponins, flavonoids, phenols, mimosine, lectin, protein, oil, calcium, phosphorus, iron, vitamin A and vitamin B. The research aimed to determine the phenolic levels found in the ethyl acetate fraction of the leaves. The extraction was done by maceration method using ethanol 96% then fractionated with ethyl acetate solvent. The obtained fraction was analysed by UV-Vis spectrophotometry method at the maximum wavelength of 761 nm using gallic acid as a standard. Based on the qualitative test, river tamarind leaves positively contained phenolic. The phenolic compound contained in the ethyl acetate fraction was 0.176 mgGAE/g in that every gram of ethyl acetate fraction had phenolic of 0.176 mg equivalent to garlic acid
IDENTIFICATION AND ANTIBACTERIAL ACTIVITY TEST OF ENDOPHYTE FUNGI MAHONI BARK (Swieenia mahagoni L.) IFKBM04
Get PDFEndophytes represent a complex community of microorganisms colonizing asymptomatically internal tissues of higher plants. Several reports have shown that endophytes enhance the fitness of their host plants by direct production of bioactive secondary metabolites. This study investigated the antibacterial activity of endophytes fungi IFKBM04 isolated from Swietenia mahagoni L. and identified macroscopic and microscopic characteristics of endophytic fungi. Based on the results of macroscopic and microscopic identification compared with the literature to determine the general characteristics of the sample. The results of this morphological identification showed that the sample of mahogany bark isolate (Swietenia mahagoni L.) code IFKMB-04 had similarities with the genus Saccharomyces sp
UJI AKTIVITAS GEL LENDIR BEKICOT (Achatina fulica) SEBAGAI PENYEMBUHAN LUKA SAYAT
Get PDFSnail mucus (Achatina fulica) has a high protein content, which includes beta agglutinin, achasin protein, acharan sulfate, and glycoconjugate. The objectives of this study was to determine differences in physical evaluation of the quality of the gel preparation against snail mucus concentration and to determine the efficacy of snail mucus gel as a wound healing. This study used an experimental research method with a sample of 4 male rats (Rattus novergicus) which were divided into 6 groups in each. The results showed that there was no effect of the physical quality of snail slime gel (Achatina fulica) on the concentration of snail slime formulated. It has been proven in the Kruskall wallis statistical test with the Sig. > 0.05. The results of the wound healing activity test showed an average value of K (+) 49.61%, K (-) 17.48%, P1 48.23%, P2 43.63%, P3 43.43%, and P4 23, 97. Values that indicate differences in K (-) and P4 are evidenced in the Post Hoc LSD test with Sig. <0.05. It can be concluded that the concentration of snail mucus does not affect the physical quality of snail mucus gel preparations and snail mucus gel with snail mucus concentration of 10% is effective in the process of wound healing
KARAKTERISASI ISOLAT BAKTERI PENGHASIL SELULOSA DARI BUAH NAGA MERAH (Hylocereus polyrhizus)
Get PDFCellulose is a natural polymer widely used for general industrial purposes. The development of science utilizes bacteria from certain fruits to produce biosellulose. Red dragon fruit (Hylocereus polyrhizus) contains a lot of sugar which can be bioconverted to cellulose by cellulose- producing lines. The research aimed to obtain bacteria from red dragon fruit with the potential to produce cellulose, then conduct morphological and biochemical characterization. The research was conducted by the isolation of bacteria from the fruit, purification, and screening tests using Hestrin-Schramm medium. The isolates obtained were characterized morphologically through macroscopic and microscopic observation and the biochemically through catalase test, carbohydrate fermentation, starch and gelatin hydrolysis, motility, and IMViC (Indole, Methyl red, Voges- Proskauer, Citrate). The results showed that 4 bacterial isolates had the potential to produce cellulose. Based on Bergey's Manual of Systematic Bacteriology, 3 bacterial genera were obtained, namely Acetobacter, Gluconacetobacter, and Azotobacter. The results of the characteristics of the isolates obtained at IBBN 2-2 were positive in the catalase test, sucrose and lactose fermentation, and methyl red. IBBN 3-1 was positive in the catalase test, sugar fermentation, starch hydrolysis, methyl red, and citrate. IBBN 3-2 was positive in the sucrose and glucose fermentation test, gelatin hydrolysis and 4-1 IBBN was positive in the sucrose fermentation test and gelatin hydrolysis
UJI AKTIVITAS EKSTRAK ETIL ASETAT DAUN KAYU JAWA (Lannea coromandelic (Houtt.) Merr.) TERHADAP PENYEMBUHAN LUKA BAKAR PADA TIKUS PUTIH (Rattus norvegicus)
Get PDFWound healing is a process of repairing skin and soft tissue when these organs are injured. One of the plants that are presumed to affect wound healing is Kayu Jawa (Lannea coromandelic (Houtt.) Merr.). This research aims to determine the healing effect of burns and the effective dose of ethyl acetate extract of Kayu Jawa’s leaves. The animal test that has been induced using a hot plate with temperature 100oC stuck at the back of mice were divided into 5 (five) groups. Group 1 Na.CMC as Negative control, Group 2 Lanakeloid-E as Positive control, Group III, IV, and V were extract ethyl acetate of Kayu Jawa’s leaves (Lannea coromandelic (Houtt.) Merr) with 3 dosage variations namely, 75 mg/kg, 150 mg/kg, and 300 mg/kg were given topically 2 times a day for 21 days. Then wound area measure using calipers. The group with 300 mg/kg dosage has the best effect for reducing wound area of burns, with statistical value not significantly different from the comparison group (Lanakeloid-E) with a percentage value of 84%
Screening of Antibacterial Activities of the Endophytic Fungi Isolated from the Leaves of Medinilla speciosa Blume
Get PDFEndophytes or endophytic fungi have been investigated as a store house of bioactive compound. This study was aimed to evaluate the potential antibacterial activity of endophytic fungi isolated from Medinilla speciosa Blume. The antibacterial test was determined by measuring the inhibition zone with disk-diffusion method. Twenty endophytes were isolated from the leaves of Medinilla speciosa Blume and identified morphologically. The results demonstrated that ten isolates showed variation in their antibacterial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633, and Shigella dysenteriae ATCC 13313. Further investigation will be needed to explore and identify the bioactive molecules of the isolated endophytic fungi
AKTIVITAS ANTIOKSIDAN EKSTRAK FERMENTAT ISOLAT FUNGI ENDOFIT BIJI BUAH KENARI (Canarium indicum L.) SECARA KLT-AUTOGRAFI
Get PDFWalnut seeds (Canarium indicum L.) contain tannin, flavonoids, carotenoids which is potentially fungtioning as antioxidants. The research aimed to examine the antioxidant activity of fermentate extract of walnut seeds endophytic fungi by autography-TLC. The result of the endophytic fungi isolation in Walnut seeds (Canarium indicum L.) obtained 5 isolates with characteristics. The test result of screening free antiradical activity obtained that isolate 4th was active as an antioxidant indicated by the presence of brown spots with the addition of 1,1-diphenyl-2-picrylhydrazyl 0.004%. Isolate 4th was fermented using Maltosa Yeast Broth (MYB) medium to obtain fermentate supernatant and mycelia. The Fermentate of supernantant of isolate 4th was extracted to obtained supernantant of ethyl acetate extract. Based on the result of free antiradical activity test of ethyl acetate fermentate extract isolate 4th by autography-TLC using ethyl acetate: methanol: water eluent (6:2:1), obtained Rf 0.78 value active as free antiradical by spraying 1,1diphenyl-2-picrylhydrazil 0.004%
PENGUJIAN AKTIVITAS EKSTRAK ETANOL KULIT BUAH KECOMBRANG (Etlingera elatior (Jack) R.M.Sm) SEBAGAI INHIBITOR TIROSINASE
Get PDFHealthy skin should have a flat surface, the texture is soft, elastic, and have the same skin color. Skin color is determined by the size, number and distribution of melanin pigment. Pigment brownish pigment melanin is to protect the skin from UV light scattering. An enzyme that plays a role in this browning reaction is tyrosinase. Based on this research is conducted on the fruit skin kecombrang to see the activity of the enzyme tyrosinase barriers and whether rind kecombrang can inhibit tyrosinase enzyme and with the aim to utilize natural materials and waste that can inhibit the enzyme tyrosinase and can be used as a skin whitheter. Research iniated by the extraction of fruit skins kecombrang using etanol. Flavonid content test on the sample using Mg powder and HCl p, the results show a color change from dark brown to yellow. So positive it contains flavonoids. Measurements were contucted on a maximum wavelength of 478,003 nm, using the substrate L-tyrosine and the enzyme tyrosinase. Using comparators hidroquinon. Of the results of this study concluded that kecombrang fruit peel ectract at a concentration of 2 mg/mL could inhibit amounted to 55,92% and a concentration of 2,5 mg/mL could inhibit amounted to 56,58
KARAKTERISASI EKSTRAK TERPURIFIKASI KELOPAK ROSELLA (Hibiscsus sabdariffa L.) DAN AKTIVITASNYA SEBAGAI ANTIHIPERTENSI PADA TIKUS SPRAGUE DAWLEY
Get PDFThe characterization was ensuring quality of traditional raw materials for drug preparations. Purification extract was a part of extract which released from compounds it was more effective for pharmacological activities. The effectivity an antihypertensive was still very rarely proven by use of traditional ingredients, especially from purified rosella. The aim of the study is characterization and knowing of calyx purified extract of rosella which is suspected to have antihypertensive activity in vivo. The method was done by purifying, characterization, determined of quercetin, and antihypertensive activity. Positive control (captopril), negative (CMC Na 0,5%), test of 12,5 dan 25 mg/kg BB. Descriptive data analysis for characterization, linear regression analysis of quercetin and antihypertensive statistical testing. The results for characterization of 1.41% water content, specific gravity 0.63% w / v, drying losses 2.82% and 2.31%. Levels of quersetin of 7.02 ± 0.15. The activity as antihypertensive result decrease in blood pressure which is indicated by a significant difference between the positive control and the test group. The 25 mg / kg body weight ETKR dose is an effective dose in reducing blood pressure. The non-specific character of purified extract of rosella calyx, FHI and through oral administration of ETKR can reduce the blood pressure of test animals with an effective dose of 25mg / kgBB. This research still requires identification of bioactive compounds responsible for these activities
Radiolabeled curcumin as β amyloid imaging and tumor targeting imaging agents
Get PDFCurcumin, a polyphenolic compound, derived from the rhizomes of Curcuma longa L. Curcumin shows potential pharmacological action against numerous disorders, including cancer, neurodegenerative, and infection diseases. Curcumin-based molecular imaging agents could be useful for early detection of Alzheimer Disease and tumor and monitor the progress of therapy. Radiolabeled curcumin and its derivatives become promising compounds as imaging agents. In this review, radiolabeled curcumin bearing radionuclides including fluorine-18, Technetium-99m, Iodine-125, and Gallium-68 are reviewed as an effort to develop curcumin-based probes not only for β amyloid imaging but also for tumor imaging