Jurnal FKIP Universitas Mataram (Fakultas Keguruan Dan Ilmu Pendidikan)
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The Analysis of Pb Content in Tilapia Fish from Taliwang Lake
Research on the heavy metal lead (Pb) is important to protect consumers from dangerous heavy metal contamination. The main objective of this research is to determine the levels of Lead (Pb) metal in the body of Tilapia Fish (Oreochromis mossambicus) obtained in Taliwang Lake. The results of this study are useful so that consumers of Tilapia fish can avoid fish that are contaminated with Pb heavy metals. Data collection for this research was carried out in Taliwang Lake. It was determined that there were 2 stations in this research, namely in the eastern and western parts of the lake. Fish were caught by using gill nets. Four fish were taken at each research station as samples. The next stage, the Tilapia fish samples were wrapped in plastic samples, which were then placed in sample boxes. The destruction of tilapia fish muscle samples was carried out in the Mataram University Analytical Laboratory and the Pb content analysis was carried out at the DLHK Environmental Laboratory West Nusa Tenggara. The Pb metal content was analyzed by using an AAS (Atomic Absorption Spectrophotometer) tool. Measurement of this heavy metal was carried out by adding concentrated HNO3 and HClO4 then heated at a temperature of 60 - 70ºC with a time span of 2-3 hours until the solution became clear. The solution sample was then measured with an AAS tool using an air-acetylene flame. The conclusion of this research was that the content of heavy metal Lead (Pb) in the tissue of Tilapia Fish (Oreochromis mossambicus) taken from Lake Taliwang waters was 0.0005 mg/kg (ppm)
Supporting Habitat Contribution to Reef Fish Diversity in Sekotong Bay, West Lombok, Indonesia
Habitat complexity plays a crucial role in shaping the diversity and structure of coral reef fish communities by offering a range of ecological niches for feeding, reproduction, and shelter. This study investigates how different levels of habitat complexity, particularly the presence of supporting habitats such as seagrass beds and mangroves, influence the richness and diversity of coral reef fish communities in Sekotong Bay, Lombok, Indonesia. Data were collected at four stations using the Underwater Visual Census (UVC) method, with observations focusing on habitat parameters, substrate complexity, and anthropogenic activity. Results showed that stations with buffer habitats —mangroves at Station III and seagrass beds at Station IV— exhibited significantly higher species richness and diversity indices compared to other stations. Seagrass beds, in particular, were associated with the highest diversity (H' = 2.95; 42 species), suggesting their key role in maintaining fish community structure. Although Station IV experienced human activity, it still supported a complete community structure, including target fish species, highlighting the ecological buffering capacity of adjacent seagrass habitats. These habitats not only function as nursery grounds and sediment filters but also sustain the ecological balance and resilience of coral reef ecosystems. The study also found that river mouths may negatively affect benthic substrate diversity due to sedimentation unless mitigated by buffer vegetation. Overall, findings underscore the importance of preserving habitat complexity and integrating seagrass and mangrove protection into coastal management strategies to enhance biodiversity, ecosystem stability, and local socio-economic resilience
Identification of Neisseria gonorrhoeae in Commercial Sex Worker Clients with and without Condom Use
This study investigates the prevalence of Neisseria gonorrhoeae among commercial sex workers and their clients in Pulau Baai, Bengkulu, with a focus on comparing infection rates between individuals who use condoms and those who do not. The primary objective is to assess the impact of condom usage on the prevalence of gonorrhea in this high-risk population. A cross-sectional observational design was employed, involving 40 male participants who had engaged with commercial sex workers in the Pulau Baai area. The participants were divided into two groups based on their condom use. Urethral secretion samples were collected and analyzed via Gram staining for Neisseria gonorrhoeae detection. The study found no cases of Neisseria gonorrhoeae in any of the samples, with all 36 condom users and 4 non-users testing negative. These results suggest no gonorrhea infections in the studied population, regardless of condom use. However, due to the limitations of the Gram staining method, further research using more sensitive diagnostic techniques and a larger sample size is recommended to verify these findings
Detection of Candida albicans Fungus in Urine of Women with Vaginal Discharge by PCR Method
Candida albicans is a type of fungus responsible for pathological vaginal discharge and the most pathogenic among other Candida fungal species. The development of PCR-based molecular techniques used for identification and characterization of fungi by isolating DNA from the quality and quantity to analyze through PCR-based applications. The purpose of this study is to determine the presence of Candida albicans fungi in urine isolate samples of women who experience vaginal discharge. The research method used was descriptive. The samples used were 10 female urine samples that experienced vaginal discharge in students of the Bachelor of Applied Medical Laboratory Technology study program of STIKES Nasional class of 2023. Based on the results of research it can be concluded that the Candida albicans fungus can be detected in the ITS1 region with a product length of 219bp and the ITS2 region with a product length of 338bp using the conventional PCR method. The concluded of this study is Candida albicans fungus can be detect in the ITS1 region with a product length of 219bp and the ITS2 region with a product length of 338bp using the conventional PCR method
Analysis of Ferritin Levels as a Risk Factor for Anemia in Adolescent Girls on the West Lombok Coast
Iron deficiency anemia (ADB) is a nutritional disorder that often occurs, especially in adolescent girls, due to increased nutrient requirements during growth and menstruation. Assessing ferritin levels is an important step in determining the status of iron stores in the body and identifying individuals at risk of iron deficiency anemia, especially in resource-constrained environments such as coastal areas. This study aims to determine the prevalence of anemia and iron deficiency in adolescent girls in the coastal areas of Lombok, Indonesia and analyze the relationship between ferritin levels as a risk factor for the incidence of iron deficiency anemia. The design of this study was a cross-sectional study conducted on 101 young women aged 15 - 19 years in the coastal areas of West Lombok. Ferritin levels were measured to determine iron deficiency status (< 15 ng/ml) and hemoglobin levels were used to determine anemia in adolescent girls (HB < 12 g/dl). The Chi-Squre and relative risk tests were used for statistical analysis of the relationship and risk between iron deficiency and the incidence of anemia. The results of the study found that the prevalence of iron deficiency was 40 (39.6%) and anemia was 21 (20.79%). Of the respondents who experienced anemia, 13 (61.9%) were iron deficiency anemia. There was a significant association between anemia and iron deficiency (p = 0.004) and an odd ratio value of 4.154 was obtained, indicating that adolescent girls with iron deficiency (low ferritin levels) had a 4.154 times greater risk of developing anemia compared to adolescent girls with normal ferritin levels
Nutritional Content of Mixed Silage Pennisetum purpureum and Sesbania grandiflora With Different Additive Fermentations
Forage consisting of grass and legumes is an important aspect of ruminant but availability is often dependent on the rainy season so preservation technology is needed, namely silage. This study aimed to determine the nutritional content of silage made from a mixture of Pennisetum purpureum and Sesbania grandiflora with different doses of fermentation additives. A Completely Randomized Design (CRD) was used, consisting of four treatments with five replications. They were P0: (75% Pennisetum purpureum + 25% Sesbania grandiflora), P1: (75% Pennisetum purpureum + 25% Sesbania grandiflora + 0.1% additive), P2: (75% Pennisetum purpureum + 25% Sesbania grandiflora + 0.2% additive), P3: (75% Pennisetum purpureum + 25% Sesbania grandiflora + 0.3% additive). Each treatment was added 2% molasses and incubated for 21 days. The results showed that the addition of fermentation additives had no significant effect (P>0.05) on DM and OM content but had a highly significant effect (P<0.01) on increasing CP, EE, and TDN content and reducing CF content. The highest average DM and OM values were observed in P0 (21.02% and 89.27%, respectively), while the highest average increases in CP, EE, and TDN were found in P3 (15.31%, 2.85%, and 50.32%, respectively), and the greatest reduction in CF was also recorded in P3 (27.89%)
Vegetable Pesticides and Planting Spattern Againt Grayak Caterpillar (Spodoptera exigua) Attacks on long Beans (Vigna Sinensis L.)
: The armyworm (Spodoptera exigua) is one of the most common plant pests that lower plant yields. The purpose of this study is to ascertain how armyworm (Spodoptera exigua) attacks on long bean plants are impacted by botanical pesticides and planting distance. This study employs experiments and is quantitative in nature. In this investigation, papaya leaf trials and garlic peels were used as plant insecticides in covert studies. The one-way ANOVA test was the statistical method employed in this investigation. The findings demonstrated that while the botanical concentration had no effect on plant height or attack severity, it significantly affected the percentage of attacks and the leaf area index. Plant height, attack intensity, attack %, and leaf area index were not significantly affected by planting distance in long bean plants. The study's conclusions include the need for more research on plant insecticides that use plant leaves to fend against attacks, particularly in lowering the incidence of armyworm attacks
Antimicrobial Activity of Starfruit Leaf Extract Against Gram-Positive and Gram-Negative Bacteria
Starfruit (Averrhoa bilimbi L.) is a plant traditionally used as medicine, mainly due to its secondary metabolite content that has the potential as an antimicrobial agent. This study aims to evaluate the antimicrobial activity of A. bilimbi leaf extract against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria. Extraction was carried out using three types of solvents: 100% ethanol, 100% ethyl acetate, and a mixture of ethanol-ethyl acetate (1:1) using the maceration method. Antimicrobial activity tests were carried out using the disc diffusion method to measure the inhibition zone of bacterial growth. The results showed that all A. bilimbi leaf extracts had inhibitory activity against both types of test bacteria. The extract with ethyl acetate solvent showed the largest inhibition zone against S. aureus and E. coli, followed by the mixed ethanol-ethyl acetate extract, and then the ethanol extract. These findings indicate that the active compounds in A. bilimbi leaves are more soluble in ethyl acetate and have the potential to be developed as natural antibacterial agents
Improving Kopay Chili Seed Germination Through Priming with Eichhornia crassipes Root Extract
Kopay chili (Capsicum annuum L. cultivar Kopay) is a local Indonesian cultivar valued for its distinctive flavor, economic potential, and adaptability to tropical climates. However, one of the major challenges in its cultivation is the low and uneven germination rate, which can affect crop uniformity and productivity. This research investigates the effect of priming with water hyacinth (Eichhornia crassipes (Mart.) Solms) root extract on the germination of kopay chili seeds (Capsicum annuum L. cultivar Kopay). The objective was to evaluate the influence of soaking duration, extract concentration, and their interaction on seed germination and early seedling growth. A Completely Randomized Design (CRD) with two factors and three replications was employed. The first factor was soaking duration: 3, 6, 9, and 12 hours; the second factor was extract concentration: 0% (control), 5%, 10%, 15%, and 20%. The results showed that a soaking duration of 3 hours significantly improved germination time, growth rate, vigor index, and root length. Extract concentration alone had no significant effect on any measured parameter. However, the interaction between soaking duration and extract concentration significantly affected germination time, vigor index, root length, and seedling dry weight. These findings suggest that short-term seed priming using water hyacinth root extract can enhance germination and early growth of kopay chili under certain conditions
Inflammatory Response of Tumor Necrosis Factor-alpha (TNF-α) in Incised Wounds of White Rats (Rattus norvegicus) Induced by Aloe vera Extract
Incised wounds are common injuries that trigger an inflammatory response, in which Tumor Necrosis Factor-alpha (TNF-α) plays a crucial role in the pathogenesis. Aloe vera has long been recognized for its wound-healing potential; however, its molecular mechanisms particularly in modulating TNF-α expression remain incompletely understood. This study aims to investigate the effect of Aloe vera extract on TNF-α expression in incised wounds of white rats. This experimental study employed a controlled group design, involving a positive control group (K+), a negative control group (K−), and a treatment group (P), each consisting of five white rats. Incised wounds were created on the dorsum of the rats, and the treatment group received topical Aloe vera extract. TNF-α expression was evaluated using immunohistochemical methods on days 3, 7, and 14 post-wounding. The treatment group that received Aloe vera extract showed a significant decrease in TNF-α expression compared to the negative control group on days 7 and 14. Aloe vera extract modulates TNF-α expression in incised wounds of white rats, indicating its potential as a therapeutic agent in wound healing through anti-inflammatory mechanisms