IR@NEIST - North East Institute of Science and Technology (CSIR)
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Carlinoside reduces hepatic bilirubin accumulation by stimulating bilirubin-UGT activity through Nrf2 gene expression
Get PDFAccu mulati on of biliru bin, prima rily because of its insol ubilit y, has bee n found to be associa ted with liver
disea ses includ ing jaundice . Free bilir ubin is insol uble; its glucuro nidation by bilirubin -UGT enz yme
(UGT1 A1) makes it soluble and elimina tes it throu gh urine and fae ces. Taki ng CCl 4 induced ra t liver
dysfun ction m odel, we demonst rated that supp ression of UGT1A 1 activity in ra t liv er increa sed ser um
biliru bin level wh ich could be reve rsed by carl inoside (Cln), a flavone glyc oside. Alth ough Cln is a flavone
compou nd, it escaped self-glucu ronid ation in the intestin e and readily absorbe d. Kineti c stud y of
mic rosoma l UGT1A 1 from HepG 2 cells sugg ested that Cln enhan ced enz yme activity by increa sing V max
with out alterin g K m. This altered V max was foun d to be due to UGT1A 1 ove rexpre ssion by Cln wh ich wa s
obser ved in both HepG 2 and rat prima ry hepa tocytes . Sin ce Nrf2 is the transc ription factor of UG T1A1,
we exam ined whethe r Cln effe ct on UG T1A1 overexp ression is media ted th rough Nrf2. In Nrf2 knock -out
cells, Cln cou ld not eleva te UG T1A1 activity indica ting Nrf2 to be its target. Cln signific antly increas ed
Nrf2 ge ne expr ession in HepG2 cells wh ich was subse quently locali zed in nuclear region . Resu lts from
Ch IP assa y show ed that Cln mar kedly augm ented Nrf2 bindi ng to UGT1A 1 prom oter that con sequentl y
enhan ced report er act ivity. Our findings therefor e show that Cln upreg ulated Nr f2 gene expr ession,
incr eased its nu clear tran slocation and stimula ted UGT1A 1 prom oter act ivity. Tota l outc ome of these
even ts brought about a signific ant increase of biliru bin glucur onida tion. Cln ther efore could be a wor thy
choice to int ervene hype rbilir ubinemi a due to liv er dysfunc tion
Dinitroaliphatics as linkers: Application in the synthesis of novel artemisinin carba-dimer
Get PDFAbstract The versatility of nitroaliphatics is demonstrated
by using it in the syntheses of artemisinin derived dimers.
A few novel artemisinin derived dimer and monomer have
been synthesized using nitroalkane as linker
Control of Fusarium wilt of tomato caused by Fusarium oxysporum f. sp. lycopersici using leaf extract of Piper betle L.: a preliminary study
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Synthesis of Nio-nanoparticles on nanoporous clay matrix and catalytic transfer hydrogenation reaction
Get PDFNi°-nanoparticles of 0–8 nm were prepared in situ by impregnation of Ni(CH3COO)2 into the nanopores of
modifiedmontmorillonite(Mt)followedbypolyolreduction.TheMtwasactivatedwithHClundercontrolled
condition for generating desired pore sizes. The porous materials were characterized by XRD, TEM, SEM,
UV–visible spectroscopy, FTIR and XPS analysis. N2 adsorption data revealed specific surface areas (BET) in
the range of 296–548 m2/g, specific pore volumes of 0.4–0.6 cm3/g and pore diameters of 0–6.8 nm.
XRD pattern of Ni°-nanoparticles revealed the formation of face centered cubic (fcc) lattice. These supported
Nio-nanoparticles show efficient catalytic activity in transfer hydrogenation of acetophenone to 1phenylethanol
with about 98% conversion, having nearly 100% selectivi
Synthesis and characterization of CdS/PVA nanocomposite thin films from a complexing agent free system
Get PDFSodium dodecyl sulfate polyacrylamide gelelectrophoresis (SDS-PAGE) and random amplified polymorphic DNA (RAPD) based genetic variation studies in eri silkworm (Samia cynthia ricini Lepidoptera: Saturniidae
Get PDFBrevibacillus laterosporus strain BPM3,a potential biocontrol agent isolated from a natural hot water spring of Assam, India
Get PDFA bacterial strain designated as BPM3 isolated from mud of a natural hot water
spring of Nambar Wild Life Sanctuary, Assam, India, strongly inhibited growth of
phytopathogenic fungi (Fusarium oxysporum f. sp. ciceri, F. semitectum, Magnaporthe
grisea and Rhizoctonia oryzae) and gram-positive bacterium (Staphylococcus
aureus). The maximum growth and antagonistic activity was recorded at 30 %E6C, pH
8.5 when starch and peptone were amended as carbon and nitrogen sources, respectively.
In greenhouse experiment, this bacterium (BPM3) suppressed blast disease
of rice by 30 1467% and protected the weight loss by 35 1456.5%. The maximum disease
protection (67%) and weight loss protection (56.5%) were recorded when the
bacterium was applied before 2 days of the pathogen inoculation. Antifungal and
antibacterial compounds were isolated from the bacterium which also inhibited the
growth of these targeted pathogens. The compounds were purified and on spectroscopic
analysis of a purified fraction having Rf 0.22 which showed strong antifungal
and antibacterial activity indicated the presence of C 14H, carbonyl group, dimethyl
group, 14CH2 and methyl group. The bacterium was characterized by morphological,
biochemical and molecular approaches and confirmed that the strain BPM3 is
Brevibacillus laterosporus
nfluence of functional groups on the adsorption behaviour of substituted-benjoic acids at the alpha-alumina / water interface
Get PDFAdsorption kinetics of hemimellitic and trimellitic acids at a fixed initial concentration of 0.30mM
onto �-alumina surfaces were carried out in batch method in the presence of 0.05mM NaCl(aq) at
pH 5 and 288.15, 298.15, 303.15, and 313.15 K. The experimental data were significantly better fitted
to pseudo-second-order kinetic equation of non-linear form in the entire time duration. The state
of equilibration time of benzenetricarboxylic acids at 298.15K follows the sequence: hemimellitic
acid < trimellitic acid < trimesic acid. Adsorption isotherms of hemimellitic and trimellitic acids were carried
out at 298.15 K, pH 5–10, and 0.05mM NaCl(aq) by varying acid concentration. The adsorption data
were fitted to Langmuir adsorption model. Adsorption density of benzenetricarboxylic acids follows the
sequence: trimesic acid < trimellitic acid < hemimellitic acid. Comparison of the adsorption densities of
benzenetricarboxylic acids with dihydroxybenzoic and monohydroxybenzoic acids indicates that (i) in
benzenetricarboxylic acids the position and equally the number of –COOH groups, (ii) the two phenolic
–OH groups at any two position in the benzene ring of dihydroxybenzoic acids, and (iii) the phenolic –OH
groups at ortho position in the benzene ring of monohydroxybenzoic acids are the governing parameters
for yielding higher adsorption density onto �-alumina surface. Exception is the 2,4-dihydroxybenzoic
acid amongst dihydroxybenzoic acids series, which yields highest adsorption density due to ortho and
para position of the phenolic –OH groups. The thermodynamic data indicate that the adsorption process
is spontaneous for both the systems. The magnitude of shifting of �s(–COO−) and �as(–COO−) after
adsorption of trimellitic and hemimellitic acids onto �-alumina surface indicate that both acids form
outer-sphere surface complexes