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Impact of abdominal aortic calcification on 90-day mortality in sepsis patients : a pilot retrospective cohort study
Get PDF滋賀医科大学博士（医学）pdfThesis or DissertationAcute Medicine & Surgery. 2021 Jan 25;8(1):e630.令和3年度thesi
Habitual dietary intake and glutamic acid in durable milk at one month postpartum
Get PDFpdfGlutamic acid (Glu), an umami component in human milk, has been suggested to play an important role in the prevention of allergies and infections in infants, but the relationship between mothers' habitual diet and Glu in human milk is not clear. Therefore, we aimed to clarify the relationship between maternal dietary habits and Glu concentrations in durable milk during the first month postpartum in 41 lactating women using a cross-sectional prospective study. Metrics included habitual dietary intake at 1 month postpartum (Self-Reported Dietary History Questionnaire), Glu concentration in durable milk whey, and plasma at 1 month postpartum. There was no correlation between Glu concentration in plasma and durable milk Glu concentration at 1 month postpartum. Food items that were correlated with plasma and milk Glu concentrations were sparse in Glu or did not contain Glu; therefore, we could not determine the relationship between Glu concentrations in durable milk and maternal dietary intake. In addition, Glu concentration in durable milk was significantly higher among multipara than primipara and among mothers with a relatively high degree of physical activity. Although the study did not show that mothers' habitual dietary intake affected milk Glu, Glu concentrations in durable milk were approximately 30 times higher than those found in plasma. Further research is needed to clarify the relationship between individual factors and milk or plasma Glu.Journal Articlejournal articl
Fut9 Deficiency Causes Abnormal Neural Development in the Mouse Cerebral Cortex and Retina
Get PDF滋賀医科大学博士（医学）pdfThesis or DissertationNeurochemical Research. 2022 Sep;47(9):2793-2804.令和4年度thesi
Relationship between the gut microbiota and bile acid composition in the ileal mucosa of Crohn's disease
Get PDFpdfBackground/Aims:
Crosstalk between the gut microbiota and bile acid plays an important role in the pathogenesis of gastrointestinal disorders. We investigated the relationship between microbial structure and bile acid metabolism in the ileal mucosa of Crohn’s disease (CD).Methods:
Twelve non-CD controls and 38 CD patients in clinical remission were enrolled. Samples were collected from the distal ileum under balloon-assisted enteroscopy. Bile acid composition was analyzed by liquid chromatography-mass spectrometry. The gut microbiota was analyzed by 16S rRNA gene sequencing.Results:
The Shannon evenness index was significantly lower in endoscopically active lesions than in non-CD controls. β-Diversity, evaluated by the UniFrac metric, revealed a significant difference between the active lesions and non-CD controls (P=0.039). The relative abundance of Escherichia was significantly higher and that of Faecalibacterium and Roseburia was significantly lower in CD samples than in non-CD controls. The increased abundance of Escherichia was more prominent in active lesions than in inactive lesions. The proportion of conjugated bile acids was significantly higher in CD patients than in non-CD controls, but there was no difference in the proportion of primary or secondary bile acids. The genera Escherichia and Lactobacillus were positively correlated with the proportion of conjugated bile acids. On the other hand, Roseburia, Intestinibacter, and Faecalibacterium were negatively correlated with the proportion of conjugated bile acids.Conclusions:
Mucosa-associated dysbiosis and the alteration of bile acid composition were identified in the ileum of CD patients. These may play a role in the pathophysiology of ileal lesions in CD patients.Journal Articlejournal articl
Gel immersion endoscopic mucosal resection for a gastric neoplasm with a background of fundic gland polyposis.
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令和4年度 基礎科学研究 研究報告
Get PDF＜化学＞
1. 草木染めの原理 / 中岡史奈子
2. 生活を支える人工軟骨 / 金岡珠希
3. 医療用テープを剥がす際の負担を軽減する / 松山峻大
＜情報学＞
4. 医療画像におけるディープラーニングの原理と応用 / 堀江敦揮
＜解剖学＞
5. レンチウイルスを用いた間葉系幹細胞の接着性亢進因子のスクリーニング / 成本彩乃
6. 分化ニューロン成熟化におけるSbno1機能の解析 / 寒出祐紀恵
＜動物センター＞
7. カニクイザル栄養膜幹細胞の性状解析① / 桑原歩夢
8. カニクイザル栄養膜幹細胞の性状解析② / 五百井里歌
＜数学＞
9. 数理医学入門 / 野崎龍一
＜物理学＞
10. 走査トンネル顕微鏡法によるアミロイドβ1−42 モノマーの構造評価 / 藤井洸太朗departmental bulletin pape
Conformational change of RNA-helicase DHX30 by ALS/FTD-linked FUS induces mitochondrial dysfunction and cytosolic aggregates.
Get PDFpdfGenetic mutations in fused in sarcoma (FUS) cause amyotrophic lateral sclerosis (ALS). Although mitochondrial dysfunction and stress granule have been crucially implicated in FUS proteinopathy, the molecular basis remains unclear. Here, we show that DHX30, a component of mitochondrial RNA granules required for mitochondrial ribosome assembly, interacts with FUS, and plays a crucial role in ALS-FUS. WT FUS did not affect mitochondrial localization of DHX30, but the mutant FUS lowered the signal of mitochondrial DHX30 and promoted the colocalization of cytosolic FUS aggregates and stress granule markers. The immunohistochemistry of the spinal cord from an ALS-FUS patient also confirmed the colocalization, and the immunoelectron microscope demonstrated decreased mitochondrial DHX30 signal in the spinal motor neurons. Subcellular fractionation by the detergent-solubility and density-gradient ultracentrifugation revealed that mutant FUS also promoted cytosolic mislocalization of DHX30 and aggregate formation. Interestingly, the mutant FUS disrupted the DHX30 conformation with aberrant disulfide formation, leading to impaired mitochondrial translation. Moreover, blue-native gel electrophoresis revealed an OXPHOS assembly defect caused by the FUS mutant, which was similar to that caused by DHX30 knockdown. Collectively, our study proposes DHX30 as a pivotal molecule in which disulfide-mediated conformational change mediates mitochondrial dysfunction and cytosolic aggregate formation in ALS-FUS.Journal Articlejournal articl
Availability of death review of children using death certificates and forensic autopsy results.
Get PDFapplication/pdfThe Model Project for Child Death Review (CDR) was initiated in Japan, but parental consent is required for detailed investigations. We proposed an alternative method to review child deaths using death certificates and forensic autopsy results when parental consent is not provided. We extracted and reviewed death certificates for the deceased younger than 18 years from among all certificates submitted in Shiga Prefecture between 2015 and 2017. In addition, we analyzed autopsy records in cases that underwent forensic autopsy. The prevalence of each cause of death was compared among age groups. The situation and circumstances of unnatural deaths were analyzed in detail. Of 131 certificates, unnatural deaths accounted for 29.7 %. The prevalence of each cause of death significantly differed among age groups. Malignant disease and suicide were most common in school-aged children and congenital disease was most common in infants. Suicide was the leading cause of unnatural death, followed by suffocation, which was most common in infants. Situations where suffocation was reported included co-sleeping with the mother and breastfeeding. Despite parental consent not being obtained, the trends of regional child deaths and the circumstances of accidental deaths were clarified by the present method. However, the results of detailed investigation were lacking. This study provided basic information for implementing detailed methods and procedures for CDR at the governmental level. To perform optimal CDR, legislation for collecting detailed information without parental consent is required.Journal Articlejournal articl