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Neuronal Populations Involved in Motor Function Show Prominent Expression of Sbno1 During Postnatal Brain Development
Get PDF滋賀医科大学博士（医学）Journal of Developmental Biology. 2025 Jan 21;13(1):3.令和7年度thesi
Impact of Lactobacillus in the uterine microbiota on in vitro fertilization outcomes
Get PDF滋賀医科大学博士（医学）Journal of Reproductive Immunology. 2023 Dec:160:104138.令和6年度thesi
Metabolic analysis of three-dimensional cultured gastrointestinal cancer cells suggests that L-arginine inhibits tumor growth by affecting the urea cycle
Get PDF滋賀医科大学博士（医学）Pathobiology. 2024 Dec 10:1-17.令和6年度thesi
Comparison of postoperative adjuvant platinum-based chemotherapy and no further therapy after radical surgery in intermediate-risk early-stage cervical cancer
Get PDF滋賀医科大学博士（医学）Journal of Gynecologic Oncology. 2025 Jan;36(1):e2.令和6年度thesi
Clinical outcomes and future fertility after uterine artery embolization for postpartum and postabortion hemorrhage
Get PDF滋賀医科大学博士（医学）Background: Postpartum hemorrhage (PPH) and postabortion hemorrhage (PAH) are life-threatening conditions.
Purpose: This study aimed to evaluate the efficacy and safety of uterine arterial embolization (UAE) for PPH and PAH and to investigate future fertility after UAE.
Material and Methods: This study included 57 consecutive patients (mean age, 34 years) who underwent UAE for PPH (n = 46) and PAH (n = 11) at our institution from January 2011 to December 2022. Technical success, nonvisualization of the peripheral portion of bilateral uterine arteries on angiography, and clinical success, complete hemostasis after UAE, were assessed. UAE-associated complications and factors related to clinical success were analyzed. Pregnancy outcomes post UAE and complications during subsequent pregnancy were investigated in 16 patients who desired fertility and were followed up for > 1 year.
Results: The technical and clinical success rates were 100% and 84.2%. Sepsis (n = 1) and uterine empyema (n = 1) were observed as severe complications. Placental disorder, bleeding within 24 hours after delivery or abortion, ≥ 1.5 shock index, ≥ 6 units of transfusion erythrocytes, and ≥ 8 obstetrical disseminated intravascular coagulation score were significantly associated with unfavorable clinical outcomes. Sixteen pregnancies were observed in 12 patients after UAE, 3 of which were miscarriages and 13 were successful live births. During pregnancy, uterine rupture (n = 1) and accreta (n = 1) were observed.
Conclusion: UAE is an effective treatment for PPH and PAH. Although UAE could preserve future fertility, careful attention should be paid to perinatal management for unusual complications.令和6年度doctoral thesi
O-GlcNAc modification in endothelial cells modulates adiposity via fat absorption from the intestine in mice
No full text滋賀医科大学博士（医学）Introduction
Endothelial cells have a crucial function in transporting and exchanging various nutrients. O-GlcNAcylation, mediated by O-GlcNAc transferase (OGT), involves the addition of N-acetylglucosamine to proteins and serves as an intracellular nutrient sensing mechanism. However, the role of O-GlcNAcylation in endothelial cells remains poorly understood.
Objective
This study investigated the role of O-GlcNAcylation in endothelial cells.
Methods
Endothelial-cell-specific Ogt -knockout mice (Ogt-ECKO) were generated by crossing Ogt-floxed mice (Ogt-flox) with VE-Cadherin Cre ERT2 mice. Ogt-ECKO mice and Ogt-flox control mice were subjected to a normal or high-fat diet, and their body weight, glucose metabolism, and lipid metabolism were examined.
Results
Ogt-ECKO mice exhibited reduced body weight compared with Ogt-flox control mice under a high-fat diet. Lipid absorption was significantly impaired in Ogt-ECKO mice. Changes in the intercellular junctions of small intestinal lacteal endothelial cells from a button-like to a zipper-like configuration were observed. Furthermore, Ogt-ECKO mice showed decreased expression of VEGFR3. The administration of a nitric oxide donor restored lipid absorption and reversed the morphological alterations in Ogt-ECKO mice.
Conclusions
These findings demonstrate the critical role of O-GlcNAcylation in endothelial cells in lipid absorption in the intestine through the modulation of lacteal junction morphology. These results provide novel insight into the metabolic regulatory mechanisms under physiological conditions and have implications for the development of new therapeutic strategies for obesity.令和6年度doctoral thesi
Impact of leptin on postoperative peritoneal adhesion formations in colorectal resection
Get PDF滋賀医科大学博士（医学）International Journal of Colorectal Disease. 2025 Mar 15;40(1):67.令和7年度thesi
Metabolic Analysis of Three-Dimensional Cultured Gastrointestinal Cancer Cells Suggests that l-Arginine Inhibits Tumor Growth by Affecting the Urea Cycle
No full text滋賀医科大学博士（医学）Introduction: There is evidence for the anticancer effects of l-arginine (arginine); however, the direct effects on cancer cells and mechanism of action are unclear. Methods: Various upper gastrointestinal cancer cells (OE19, OE33, MKN1, MKN45, MKN74, and AGS) were divided into arginine-treated and -untreated groups and cultured using two-dimensional and three-dimensional culture systems. Proliferation was evaluated using the MTT assay to identify arginine-sensitive (OE33) and arginine-insensitive (OE19) strains. Furthermore, the effects of arginine were evaluated using a mitochondrial stress test, cell cycle assay, comprehensive metabolic analysis, and tracer study using (13C6) l-arginine. Results: In OE33 (but not in OE19), the maximal respiratory capacity of mitochondria was lower in the treated group than in the control group. In OE33, S phase cells (determined using BrdU) were significantly reduced. In a comprehensive metabolic analysis of OE33, citrulline/ornithine levels were significantly lower in arginine-treated than in untreated cells. Using OE33, carbamoyl aspartic acid (CAA) levels were significantly lower in arginine-treated than in untreated cells. A tracer study suggested that arginine promotes the urea cycle. Conclusion: Arginine affected urea cycle metabolism, thereby decreasing CAA, which is required for pyrimidine nucleotide synthesis. These findings provide insight into the mechanism underlying the anticancer effects of arginine.令和6年度doctoral thesi
Characterization of the gut phageome of Japanese patients with ulcerative colitis under endoscopic remission
No full text滋賀医科大学博士（医学）This study aimed to analyze the gut phageome in Japanese patients with ulcerative colitis (UC) in endoscopic remission. Fecal samples were collected from 35 UC patients and 22 healthy controls. The gut microbiome was analyzed using 16S rRNA amplicon sequencing, and the phageome was profiled through shotgun metagenomic sequencing. Compared to healthy controls, UC patients showed a significant reduction in phageome richness (observed species and Chao1 index). Principal coordinate analysis revealed a significant difference in beta-diversity between UC and healthy controls (p = 0.001). The abundance of temperate phages was higher in UC (15.2%) compared to healthy controls (5.9%), although this was not statistically significant (p = 0.088). Temperate phages associated with Coprococcus sp., Bacteroides sp. KFT8, and Faecalibacterium prausnitzii, as well as virulent phages associated with Ruminococcus gnavus and Lactobacillus farciminis, were increased in UC patients. Conversely, phages associated with Thermosipho affectus, Bacteroides sp. OF03-11BH, and Odoribacter splanchnicus were decreased in UC patients. Phages associated with the genera Odoribacter (p = 0.0004), Ruminococcus (p = 0.009), and Veillonella (p = 0.013) were significantly reduced in UC patients. The gut phageome of inactive UC patients exhibited notable alterations in viral composition compared to healthy controls. These results suggest that changes in the gut phageome might be involved in the pathogenesis of UC.令和6年度doctoral thesi