Shiga University of Medical Science Repository BI WAKO / 滋賀医科大学機関リポジトリ
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Nardilysin in vascular smooth muscle cells controls blood pressure via the regulation of calcium dynamics
Get PDFBlood pressure is a crucial physiological parameter and its abnormalities can cause a variety of health problems. We have previously reported that mice with systemic deletion of nardilysin (NRDC), an M16 family metalloprotease, exhibit hypotension. In this study, we aimed to clarify the role of NRDC in vascular smooth muscle cell (VSMC) by generating VSMC-specific Nrdc knockout (VSMC-KO) mice. Our findings reveal that VSMC-KO mice also exhibit hypotension. Aortas isolated from VSMC-KO mice exhibited a weakened contractile response to phenylephrine, accompanied by reduced phosphorylation of myosin light chain 2 and decreased rhoA expression. VSMC isolated from VSMC-KO aortas showed a reduced increase in intracellular Ca2+ concentration induced by α-stimulants. These findings suggest that NRDC in VSMC regulates vascular contraction and blood pressure by modulating Ca2+ dynamics.journal articl
Expression of proinflammatory cytokines and proinsulin by bone marrow-derived cells for fracture healing in long-term diabetic mice
Get PDF滋賀医科大学博士（医学）Background:
Diabetes mellitus (DM) causes bone dysfunction due to poor bone quality, leading to severe deterioration in patient of quality of life. The mechanisms of bone metabolism in DM remain unclear, although chemical and/or mechanical factors are known to disrupt the homeostasis of osteoblasts and osteoclasts. The purpose of this study was to identify the changes of osteoblasts and osteoclasts under long-term hyperglycaemic conditions, using a mouse fracture model of long-term hyperglycemia (LT-HG).
Methods:
C57BL/6J mice and green fluorescent protein (GFP) -positive bone marrow transplanted C57BL/6J mice with LT-HG, maintained under a state of hyperglycaemia for 2 months, were used in this study. After the experimental fracture, we examined the immunohistochemical expression of proinsulin and tumor necrosis factor (TNF) -α at the fracture site. C57BL/6J fracture model mice without hyperglycaemia were used as controls.
Results:
In the LT-HG mice, chondrocyte resorption was delayed, and osteoblasts showed an irregular arrangement at the callus site. The osteoclasts were scattered with a decrement in the number of nuclei. The expression of proinsulin was confirmed in bone marrow derived cells (BMDCs) with neovascularization 2 and 3 weeks after fracture. Immunopositivity for TNF-α was also confirmed in immature chondrocytes and BMDCs with neovascularization at 2 weeks, and the number of positive cells was not decreased at 3 weeks. Examination of GFP-grafted hyperglycaemic mice showed that the majority of cells at the fracture site were GFP-positive. Immunohistochemistry showed that the rate of double positives was 15% for GFP and proinsulin and 47% for GFP and TNF-α.
Conclusion:
LT-HG induces an increase in the number of proinsulin and TNF-α positive cells derived from BMDCs. We suggest that proinsulin and TNF-α positive cells are involved in both bone formation and bone resorption after fracture under hyperglycaemic conditions, resulting in the delay of bone healing.令和5年度doctoral thesi
シャカイ フッキシタ ウツビョウ カンジャ ノ ハイグウシャ ガ ケイケンシタ ニチジョウ セイカツ ニ オケル コンナンカン
Get PDF【目的】
社会復帰したうつ病患者の配偶者が抱く日常生活における困難感を、現象学的手法を用いて記述する。
【方法】
研究対象はうつ病と診断された30歳以上70歳未満の通院治療中の患者の配偶者とし、半構成的面接を行った。分析方法には現象学的手法を用いて困難感に関するテーマを明らかにした。
【結果】
参加者は、60代女性計3名（Aさん、Bさん、Cさん）であった。うつ病患者の配偶者の困難感を表すテーマとして3名の研究参加者より計20のサブテーマが示された。そのうち７つのサブテーマから２つの共通する困難感のテーマと、13の個別の特徴をもつサブテーマが導きだされた。とくに共通するテーマは、自分の配偶者のうつ病に対して、現状の受け入れと否定を繰り返す不規則性を持つ「なんでというやる瀬のない思い」や、うつ病となった配偶者に対しこれまでの夫婦として過ごしてきた時間から生じる葛藤も含む「配偶者としての患者との接し方がわからない」という困難感を示していた。
【考察】
看護師はこれらうつ病患者の配偶者の困難な体験を患者家族がうつ病を認識し、積極的な介入を行っていく必要がある。[Purpose]
This study aimed to elucidate the hardships faced by spouses of individuals diagnosed with depression in their daily lives, utilizing phenomenological methods.
[Method]
The research focused on spouses of patients aged 30 to 70 undergoing outpatient treatment for depression, conducting semi-structured interviews to gather qualitative data. The phenomenological method was employed for analysis to identify themes associated with feelings of hardships.
[Results]
The participants were three women in their 60s (Participant A, Participant B, and Participant C). A total of 20subthemes were identified by the three research participants as themes expressing the difficulties faced by spouses of depressed patients. From these seven subthemes, two common themes of difficulty and 13 subthemes with individual characteristics were derived. Particularly common themes are ``why not?'' with irregularities of repeatedly accepting and denying the current situation when dealing with one's spouse's depression; The patient expressed a sense of difficulty, including conflicts arising from the time they had spent together as a married couple, saying, ``I don't know how to interact with the patient as a spouse.''
[Discussion]
Nurses need to make the patient's family aware of the difficult experiences of the spouses of these depressed patients and provide active intervention.departmental bulletin pape
Cases of pleural effusion possibly due to clinical pleuroperitoneal communication in the perioperative period of emergency gynecologic surgery: Case series and literature review
Get PDFPleuroperitoneal communication poses a respiratory failure risk due to pleural fluid accumulation with thoracic migration of ascites. Here, we discuss the following cases: Case 1: A woman was diagnosed with a ruptured ovarian tumor with right pleural fluid and ascites, without respiratory failure. Ovarian cystectomy was performed with inadequate removal of ascites. Postoperatively, respiratory failure occurred, and thoracentesis detected pleural fluid resembling ascites. Case 2: A woman was diagnosed with a ruptured ectopic pregnancy with right pleural fluid and ascites without respiratory failure. A diagnosis of clinical pleuroperitoneal communication was considered based on computed tomography findings. During laparoscopic salpingectomy, high-pressure ventilation was performed to push the pleural fluid back into the abdominal cavity; a negative-pressure drain was inserted, and the ascites was completely removed. Postoperative radiography revealed the absence of pleural fluid. Therefore, a preoperative diagnosis of clinical pleuroperitoneal communication and appropriate intraoperative techniques can prevent postoperative respiratory failure.journal articl