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Assessment of the tolerance of Listeria innocua isolates recovered from meat products and food-processing environments in Poland to benzalkonium chloride
No full text[ENG:]14 files containing experimental data were deposited in the database. These include:a file with data on the isolates, their origin, tolerance profiles, and the presence of resistance genes (Data_on_isolates_tolerance_profiles.csv);three files containing images of microbiological cultures showing growth inhibition of isolates on BHI medium for three technical replicates (Images_of_growth_inhibition_of_isolates_BHI_1st_technical_replicate.zip, Images_of_growth_inhibition_of_isolates_BHI_2nd_technical_replicate.zip, Images_of_growth_inhibition_of_isolates_BHI_3rd_technical_replicate.zip);and three files containing images of microbiological cultures showing growth inhibition of isolates on M-H medium supplemented with 1.2% sheep blood for three technical replicates (Images_of_growth_inhibition_of_isolates_M-H_+_1.2%_sheep_blood_1st_technical_replicate.zip, Images_of_growth_inhibition_of_isolates_M-H_+_1.2%_sheep_blood_2nd_technical_replicate.zip, Images_of_growth_inhibition_of_isolates_M-H_+_1.2%_sheep_blood_3rd_technical_replicate.zip).In addition, the dataset includes four sets of files presenting the results of PCR agarose gel electrophoresis for individual resistance genes: Electrophoresis_results_for_the_qacE_gene.zip, Electrophoresis_results_for_qacF_gene.zip, Electrophoresis_results_for_the_qacG_gene.zip, Electrophoresis_results_for_the_qacH_gene.zip, Electrophoresis_results_for_the_qacJ_gene.zip, Electrophoresis_results_for_the_brcABC_gene.zip i Electrophoresis_results_for_the_ermC_gene.zip.The files were saved in .csv, .jpg, and .png formats and were prepared using Microsoft Word and Microsoft Excel. The data present the results of studies on the analysis of tolerance to benzalkonium chloride, a disinfectant widely used in the food industry, in 51 Listeria innocua bacterial isolates originating from raw and processed meat products as well as from meat-processing plant environments. Tolerance was determined using the agar diffusion method on two media: Brain Heart Infusion (BHI) agar and Mueller–Hinton (M-H) agar supplemented with 1.2% sheep blood, at BC concentrations of 0, 5, 10, 15, 20, 25, 30, 35, 40, and 45 µg/mL, which enabled the determination of minimum inhibitory concentrations (MICs). In addition, the presence of seven selected resistance genes to quaternary ammonium compounds (QACs), including benzalkonium chloride (qacE, qacF, qacG, qacH, qacJ, brcABC, and ermC), was analyzed using the PCR technique.[PL:]W bazie danych zdeponowano 14 plików zawierających dane eksperymentalne. Obejmują one:Plik z danymi dotyczącymi izolatów, ich pochodzenia, profili tolerancji oraz obecności genów oporności: Data_on_isolates_tolerance_profiles.csv,Trzy pliki zawierające zdjęcia posiewów mikrobiologicznych z zahamowaniem wzrostu izolatów na podłożu BHI dla trzech powtórzeń technicznych: Images_of_growth_inhibition_of_isolates_BHI_1st_technical_replicate.zip, Images_of_growth_inhibition_of_isolates_BHI_2nd_technical_replicate.zip, Images_of_growth_inhibition_of_isolates_BHI_3rd_technical_replicate.zip,Trzy pliki zawierające zdjęcia posiewów mikrobiologicznych z zahamowaniem wzrostu izolatów na podłożu M-H + 1,2% krwi baraniej dla trzech powtórzeń technicznych: Images_of_growth_inhibition_of_isolates_M-H_+_1.2%_sheep_blood_1st_technical_replicate.zip, Images_of_growth_inhibition_of_isolates_M-H_+_1.2%_sheep_blood_2nd_technical_replicate.zip, Images_of_growth_inhibition_of_isolates_M-H_+_1.2%_sheep_blood_3rd_technical_replicate.zip.Ponadto zbiór zawiera cztery zestawy plików przedstawiających wyniki elektroforezy agarozowej PCR dla poszczególnych genów oporności: Electrophoresis_results_for_the_qacE_gene.zip, Electrophoresis_results_for_qacF_gene.zip, Electrophoresis_results_for_the_qacG_gene.zip, Electrophoresis_results_for_the_qacH_gene.zip, Electrophoresis_results_for_the_qacJ_gene.zip, Electrophoresis_results_for_the_brcABC_gene.zip i Electrophoresis_results_for_the_ermC_gene.zip.Pliki zapisano w formatach .csv, .jpg oraz .png, a zostały przygotowane przy użyciu programów Microsoft Word oraz Microsoft Excel. Dane przedstawiają wyniki badań nad analizą tolerancji 51 izolatów bakterii Listeria innocua, pochodzących z surowych i przetworzonych produktów mięsnych oraz ze środowiska zakładów przetwórstwa mięsa, na chlorek benzalkoniowy, środek dezynfekcyjny powszechnie wykorzystywany w przemyśle spożywczym. Tolerancję oznaczano metodą metodą dyfuzji w agarze na dwóch podłożach: agarze Brain Heart Infusion (BHI) oraz agarze Mueller–Hinton (M-H) suplementowanym 1,2% krwi owczej, przy stężeniach BC wynoszących 0, 5, 10, 15, 20, 25, 30, 35, 40 i 45 µg/mL, co umożliwiło określenie minimalnych stężeń hamujących (MIC). Przeprowadzono także analize obecności siedmiu wybranych genów oporności na czwartorędowe sole amoniowe (QAC), do których należy chlorek benzalkoniowy (qacE, qacF, qacG, qacH, qacJ, brcABC i ermC), z z zastosowaniem techniki PCR.</p
Wykaz otwartych danych artykułu: Seks, młodzież i panika moralna w międzywojennym polskim mieście na przykładzie Tarnowa
No full textNiniejszy zbiór danych zawiera listę źródeł, jakie zostały użyte w artykule, jak i również kolekcje linków do bibliotek cyfrowych (głównie jagiellońska biblioteka cyfrowa, ale także i polona.pl) odsyłających do zdigitalizowanych źródeł. Zbiór zawiera także kolekcję linków do bibliotek i repozytoriów cyfrowych odsyłających do literatury przedmiotu użytej do badań. Wskazane w wypisach kolekcje cyfrowe są udostępniane na zasadach Open Access.W artykule podjęto analizę sposobów postrzegania oraz kwestionowania seksualności młodzieży w międzywojennym Tarnowie, średniej wielkości, etnicznie zróżnicowanym mieście II Rzeczypospolitej, ze szczególnym uwzględnieniem roli religii, procesów modernizacyjnych oraz dyskursu publicznego. Na podstawie prasy katolickiej, żydowskiej i świeckiej, a także materiałów wspomnieniowych, zbadano, w jaki sposób seksualność funkcjonowała jednocześnie jako temat tabu oraz źródło paniki moralnej. Publikacje katolickie w szczególny sposób wzmacniały tradycyjne role płciowe, akcentując znaczenie czystości i prokreacji, a zarazem przestrzegając przed wpływem kina, mody i miejskiej nowoczesności. Z kolei prasa żydowska częściej przedkładała kwestie mobilizacji politycznej nad otwartą debatę na temat seksualności. Pomimo instytucjonalnych prób kontroli zachowań intymnych, młodzi ludzie pozyskiwali wiedzę o seksualności poprzez media świeckie, kulturę popularną oraz relacje rówieśnicze, nierzadko negocjując praktyki bardziej liberalne, niż dopuszczały to oficjalne normy. Wspomnienia dodatkowo uwydatniają istotną rolę kobiet w kształtowaniu życia intymnego, komplikując utrwalone współcześnie stereotypy. Tarnów jawi się zatem jako studium przypadku napięć między konserwatywnymi dyskursami religijnymi a zmieniającymi się praktykami intymności w międzywojennej Polsce.</p
Data accompanying the chapter 'The Semiotics of Scientific Scrutiny: Unveiling Peer Review through Digital Symptomatology'
No full textThis dataset accompanies the chapter 'The Semiotics of Scientific Scrutiny: Unveiling Peer Review through Digital Symptomatology' (under review). It was generated using our Genre Modeler package (https://github.com/cognitive-metascience/genre-modeler) based on open corpora. The dataset contains also a visualization script for Figure 5 and the data exported from Sketch Engine for the MDPI 2 Peer Review corpus collocations of the verb "lack". The corpus is also available on RePOD: https://repod.icm.edu.pl/dataset.xhtml?persistentId=doi:10.18150/SHKP7B</p
Electrophoretically deposited nano TiO2/Ag powder to protect fuel cell Ni cathode from dissolution in molten carbonate
No full textData deposited in the form of an Excel file containing precise numerical results of performance test (current-voltage dependence), electrical conductivity measurement, and solubility tests.The functional properties of a porous nickel cathode from MCFC fuel cell produced by tape casting and reinforced with Ni foam were enhanced by electrophoretic deposition (EPD) of TiO2/Ag particles. The obtained results demonstrate a significant improvement in cell efficiency, with power output increasing from 90 mW/cm2 for the reference Ni cathode to 100 mW/cm2 for the modified cathode. Furthermore, cathode solubility was reduced by 300 %.</p
Nanoplatform based on SW480-derived exosome nanocarriers for efficient doxorubicin hydrochloride drug loading
No full textThe collection contains raw data generated during the successful isolation of exosomes from the human colon cancer cell line SW480 and their subsequent loading with doxorubicin hydrochloride as a drug-delivery nanoplatform. The size distribution of exosomes was obtained using the nanoparticle tracking assay (NTA). The loading of exosomes with doxorubicin was monitored using fluorescence spectroscopy and the standard addition method. The viability of HBE cells after exosomes loaded with doxorubicin uptake was evaluated using Alamar Blue assay.</p
Data for manuscript entitled 'Cavity-enhanced spectroscopy in the deep cryogenic regime for quantum sensing and metrology'
No full textThe contents of this dataset allows one to recreate the results from aforementioned article.</p
Validation of LC-MS/MS method for analysis of ruxolitinib and its metabolites in human plasma
No full textThe aim of the study was to develop and validate a UPLC-MS/MS method for determining the concentrations of ruxolitinib and its two main metabolites, M18 and M27, in blood plasma.Optimization of chromatographic separation included the selection of the stationary phase, separation temperature, and solvent for sample preparation. For further studies, a Luna Phenyl-Hexyl column and a mobile phase consisting of a mixture of 0.1% formic acid in water and acetonitrile in a gradient flow were used. The separation was performed at 40°C, and sample preparation involved protein precipitation with methanol and filtration. A triple quadrupole mass spectrometer was used for analyte detection after prior electrospray ionization. The full method validation was carried out in accordance with the ICH M10 guidelines. The following validation parameters were calculated: linearity, lower limit of quantification, precision, accuracy, matrix effect, stability.</p
Photocatalytic Deaminative Fluorination—Balancing Conflicting Chemical Properties
No full textThe transformation of amines into alkyl fluorides is an important but highly underdeveloped process that would be useful for the synthesis of biologically active compounds. In this article, we describe the path to the development of deaminative fluorination under photocatalytic conditions. Through optimization studies, focusing on mechanistic complexities, we were able to determine crucial parameters influencing this reaction course.</p
Hydrogen gas sensing performance of p-type copper and titanium mixed oxides: influence of the elemental composition and the annealing temperature
No full textFiles include the data presented in the manuscript entitled: Hydrogen gas sensing performance of p-type copper and titanium mixed oxides: influence of the elemental composition and the annealing temperature by E. Mankowska and M. Mazur.Fig. 4. Diffractograms of thin films of a) TiOx, b) (Cu0.23Ti0.77)Ox, c) (Cu0.41Ti0.59)Ox, d) (Cu0.56Ti0.44)Ox, e) (Cu0.77Ti0.23)Ox and f) CuxO after deposition and after annealing.Fig. 6. a) Summary of the size of selected TiO2 crystallites depending on the amount of copper in the thin film and annealing temperature, b) crystallization temperature of anatase depending on the elemental composition of the thin filmFig. 7. Summary of the size of selected crystallites depending on the amount of copper in the thin film and the annealing temperature.Fig. 8. Sensor response of thin films with various elemental composition annealed at 200°C, 250°C and 300°C and measured at various operating temperatures.Fig. 9. The dynamic of resistance changes of thin films a) TiOx, b) (Cu0.41Ti0.59)Ox, c) (Cu0.56Ti0.44)Ox, d) (Cu0.77Ti0.23)Ox and e) CuxO to 100, 200, 500 and 1000 ppm of H2 measured at operating temperatures from 200 to 300°C.Fig. 10. Sensor response to 100 ÷ 1000 ppm of hydrogen of copper and titanium oxides based thin films annealed at 300°C, measured at operating temperature of a) 250°C and b) 300°C.Fig. 11. Repeatability test of (Cu0.77Ti0.23)Ox and CuxO thin films annealed at 300°C and measured at 250°C upon exposure to 200 ppm of H2.Fig. 12. XPS spectra of Ti2p region of TiOx thin film after annealing at 200°C and after exposition to hydrogen for 15 min and 60 min.Fig. 13. XPS spectra of a-c) Cu2p and d-f) Cu LMM region of thin film (Cu0.56Ti0.44)Ox, (Cu0.77Ti0.23)Ox, and CuxO, respectively.</p
Utilization of post-fermentation sludge as a soil structure and strength conditioner
No full textThe deposited dataset was collected as part of the research project “Wpływ biowęgla wzbogaconego mikrobiologicznie na właściwości fizykochemiczne i biologiczne agregatów glebowych – badania modelowe” (“The influence of microbiologically enriched biochars on the physicochemical and biological properties of soil aggregates – model studies”), funded by the National Science Centre, Poland, under the SONATA 17 program (project no. 2021/43/D/ST10/01656). The project is based on model aggregates prepared from biochar and biochar enriched with microorganisms (Trichoderma), mixed with soils of different texture classes and physicochemical characteristics. The research in the project covers bulk density, aggregate structure and pore characteristics, water and mechanical stability of aggregates, wettability, microbial activity, as well as a range of other physicochemical and biological parameters of soils.The study was carried out between December 2023 and June 2024 at the Institute of Agrophysics, Polish Academy of Sciences, in Lublin.The dataset includes results for six soil types, post-fermentation sludge, and mixtures of soils amended with digestate at various proportions (1–15%). The deposited data comprise the following parameters:Total carbon content;pH measured in H2O and KCl;Organic carbon content;Solid-phase density;Bulk density;Specific surface area;Cation exchange capacity;Average pore diameter;Pore surface area;Total pore volume;Total porosity; Granulometric composition;Ash content;Content of available macronutrients and heavy metals;Maximum force causing aggregate destruction;Young’s modulus;Mercury intrusion porosimetry cumulative curves;Mercury intrusion porosimetry differential curves;Stress-strain curves.The dataset consists of the following *.xlsx files: A_1 – pH measured in H₂O for six soils (Abruptic Luvisol (A), Brunic Arenosol (B), Haplic Cambisol (C), Haplic Chernozem (D), Haplic Fluvisol (E), Stagnic Luvisol (F)), post-fermentation sludge, and soil–digestate mixtures at proportions of 0, 1, 3, 5, 10, and 15%; A_2 – pH measured in KCl for the same soils, post-fermentation sludge, and soil–digestate mixtures (0, 1, 3, 5, 10, and 15%); A_3 – Total carbon content of six soils and post-fermentation sludge; A_4 – Organic carbon content of six soils; A_5 – Solid phase density of six soils and post-fermentation sludge; A_6 – Bulk density of six soils, post-fermentation sludge and soil–digestate mixtures (0, 1, 3, 5, 10, and 15%); A_7 – Specific surface area of six soils, post-fermentation sludge, and soil–digestate mixtures (0, 1, 3, 5, 10, and 15%); A_8 – Cation exchange capacity (CEC) of six soils; A_9 – Average pore diameter obtained by mercury intrusion porosimetry for six soils, post-fermentation sludge, and soil–digestate mixtures (0, 1, 3, 5, 10, and 15%); A_10 – Surface area of pores obtained by mercury porosimetry method for six soils, post-fermentation sludge, and soil–digestate mixtures (0, 1, 3, 5, 10, and 15%); A_11 – Pore volume obtained by mercury porosimetry method for six soils, post-fermentation sludge, and soil–digestate mixtures (0, 1, 3, 5, 10, and 15%); A_12 – Total porosity obtained by mercury porosimetry method for six soils, post-fermentation sludge, and soil–digestate mixtures (0, 1, 3, 5, 10, and 15%); A_13 – Granulometric composition of six soils; A_14 – Organic carbon content of post-fermentation sludge; A_15 – Ash content of post-fermentation sludge; A_16 – Content of available macronutrients and heavy metals in post-fermentation sludge; A_17 – Cumulative mercury intrusion porosimetry curves for six soils, post-fermentation sludge, and soil–digestate mixtures (0, 1, 3, 5, 10, and 15%); A_18 – Differential mercury intrusion porosimetry curves for six soils, post-fermentation sludge and soil–digestate mixtures (0, 1, 3, 5, 10, and 15%); A_19 – Stress–strain curves for six soils, post-fermentation sludge, and soil–digestate mixtures (0, 1, 3, 5, 10, and 15%); A_20 – Maximum force required to destroy aggregates obtained for six soils, post-fermentation sludge, and soil–digestate mixtures (0, 1, 3, 5, 10, and 15%); A_21 – Young’s modulus determined for six soils, post-fermentation sludge, and soil–digestate mixtures (0, 1, 3, 5, 10, and 15%);Summary of methods and equipment: A_1, A_2 – CX-505 digital pH meter (Elmetron); A_3 – TOC MULTI N/C 2000 analyzer with HT 1300 module (Analytik Jena); A_4 – Dichromate oxidation method; A_5 – Helium Ultrapycnometer 1000 (Quantachrome); A_6 – Bulk density calculated by dividing aggregate mass by aggregate volume measured via mercury immersion; A_7 – 3Flex surface area analyzer (Micromeritics, Norcross, GA, USA); A_8 – CEC was determined using an indirect method by separately measuring the sum of basic cations (Ca²⁺, Mg²⁺, K⁺, and Na⁺) and exchangeable hydrogen (H⁺); A_9–A_12, A_17, A_18 – Autopore IV 9500 mercury porosimeter (Micromeritics, Norcross, GA, USA); A_13 – The aerometric method with the Prószyński modification; A_14 – The Alten method, based on the oxidation of organic matter in a sulfuric acid (VI) environment; A_15 – Ash content determined by heating samples at 550 °C for 6 h in a muffle furnace (FCF 12 SP, Czylok); A_16 – Phosphorus: UV–Vis spectrophotometry (SPECORD 50 PLUS, Analytik Jena); Potassium, magnesium, calcium: atomic absorption spectrometry (AAS contra AA 300, Analytik Jena); Zinc, lead, chromium, cadmium, copper: acid digestion (HNO₃–H₂O₂–HCl) followed by AAS (contra AA 300, Analytik Jena); A_19–A_21 – Mechanical testing machine LabTest 6.10.1 (LABORTECH s.r.o., Opava, Czech Republic).</ul