Biology, Medicine, & Natural Product Chemistry (E-Journal - Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity)
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Stability of T-DNA Integration in Phalaenopsis “Sogo Vivien†Transgenic Orchid Carrying 35S::Gal4::AtRKD4::GR
Get PDFOrchid is an elegant ornamental plant and favoured by the society. Phalaenopsis "Sogo vivien" is a mini-sized orchid with an interesting white-striped purple petals. This study was aimed to analyze the stability of the integration of embryonic gene carrier T-DNA from Arabidobsis AtRKD4 into the P. "Sogo vivien" genome produced in 2016. The study was conducted in 3 stages: 1) Transgenic plant phenotype analysis (1 year old); 2) Examination of T-DNA integration in orchid genotypes using PCR. 3) Analysis of transgenic plant leaf explants’ ability to produce somatic embryo in vitro. In vitro cultures were performed on the base medium of New Phalaenopsis (NP), plus various concentrations of TDZ (0, 1, 2 mg.L-1) and IBA (0, 1, 2 mg.L-1) or without TDZ and IBA as controls. The transgenic Phalaenopsis ‘Sogo vivien’ were transferred to pot mediums via ex vitro with two treatments: the first leaves were cut as explants for in vitro culture, and the plants were transferred to the mixture of fern medium with shavings of bark. The integration of T-DNA in the genome was detected by DNA genome amplification from the second leaves using the AtRKD4 gene primers and the POH1 gene. The results showed that the highest number of somatic embryo (SE) propagules or protocorm like bodies (PLBs) amounted to 27 were derived from transgenic plant # 2 cultured on NP + 2 mg.L-1 TDZ +1 mg.L-1 IBA medium. The presence of AtRKD4 transgenes were detected with the amplification of 380 bp of the RKD4 gene from the genome of transgenic plant # 2 by using PCR. There were 2 out of 15 plants that positively carry the AtRKD4 gene and produce SE. Thus, the stability of the AtRKD4 carrier T-DNA integration in the genomes of transgenic plants was 13.3%
Anatomical Study of Male Reproductive Organs of the Indonesian Short-Nosed Fruit Bat (Cynopterus titthaecheilus Temminck, 1825)
Get PDFBats are one of the mammals of Chiroptera order. Chiroptera Order has two sub-orders, Megachiroptera and Microchiroptera. One of the species of Megachiroptera is Cynopterus titthaecheilus (C. titthaecheilus). Local name for C. titthaecheilus is the Indonesian short-nosed fruit bat. Characteristics of C. titthaecheilus are dark brown body hair and no tragus in the ear. Population of C. titthaecheilus in Indonesia are relatively abundant, Â but research about anatomy of male reproduction organs of this species is still rare. The purpose of this study was to determine the anatomical features of male C. tittahecheilus reproductive organs. Observations of macroscopic anatomy include observation of morphology, size, weight and volume of penis, testiscle, epididymis, vas defferens, prostate and seminal vesicles. Observation on microscopical anatomy of male reproduction organ is using histological slide preparations stained by Hematoxylin-Eosin
Comparative Anatomy and Histology of Black Pomfret (Formio niger) and Nile tilapia (Oreochromis niloticus) Kidney
Get PDFBlack pomfret and nile tilapia are belonging to order Perciformes. Both fish are live in different habitat. Black pomfret is marine water whereas nile tilapia is in freshwater. The purpose of the study was to determine anatomy and histology of the kidneys structures in both fishes. Histological preparations were done using paraffin method, with Hematoxylin-Eosin (HE) staining. The results, showed that Black pomfret and nile tilapia have y-shape kidney. Nile tilapia has darker red colour and softer texture than black pomfret kidney. Histologically, black pomfret and nile tilapia kidneys have a distal tubule, proximal tubule, glomerulus and lymphoid tissue. Glomerular diameter of nile tilapia (69,22 µm) was larger than pomfret (61,25 µm). It can be concluded that differences between anatomical and histological structure of kidney are affected by habitat differences
The Female Population Growth Projection Year 2021 in Trenggalek Regency by Leslie Matrix Model on the Birth Rate and Life Expectancy
Get PDFThis research aims to determine the number of female residents in Trenggalek Regency in 2021 based on data on birth rate and life expectancy. The use of eigenvalues and eigenvectors aims to determine the dividing age distribution by Leslie matrix model. The eigenvectors are used to determine the number of female populations of each age interval, while the eigenvalues are used to determine population growth rates. The research method used is to determine the subject of research. The next stage is to collect research data, then analyze the data and last draw conclusions. The research data is obtained from BPS Kabupaten Trenggalek and BPS East Java Province that is data of woman population from year 2010-2015. The result of this research using Leslie matrix model for female population in Trenggalek Regency that is discrete model. The discrete model is divided into fourteen age intervals constructed using the birthrate and life expectancy. The conclusions of the study showed that the number of female population in Trenggalek Regency tended to increase with positive eigen value greater than one. In other words, the growth rate of female population in Trenggalek Regency tends to be positive. The success of Leslie's matrix model is the application of case studies in predicting the number of female populations in Trenggalek District by 2021 using the MAPLE 16 Program
Vitamin C and Total Sugar Content Characterization on 31 Accessions of Banana Collection of Banana Germplasm Plants of Yogyakarta
Get PDFBanana is one of the tropical fruits that people like because it tastes good and contains good nutritional value that beneficial for health. The content of vitamin C and total sugar are an important character to complete the morphological characterization of banana accession that can be utilized by breeders in choosing accession as parent. The study aimed to determine the content of vitamin C and total sugar on 31 accessions of banana collection of Banana Germplasm Plants of Yogyakarta. The research was conducted at Banana Germplasm Plantation and Laboratory of Agricultural Technology Production of Gadjah Mada University, Yogyakarta from January to December 2016. The results indicated that 31 banana accessions showed their susceptibility to vitamin C content 60.42 + 39.22 mg / 100 g and total sugar 22.06 + 16.01%. High standard deviation values indicate the large diversity of banana accessions that were characterized, indicating that the accessions of each characterized banana were separate accessions of one another
Alliin as a Natural Bioactive from Single Bulb Garlic (Allium sativum) for Nitric Oxide (NO) Increasing in Atherosclerotic Process Based on Insilico Screening
Get PDFAtherosclerosis is a chronic inflammatory disease in the arterial wall mediated by proinflammatory factor. Atherosclerosis cause heart disease and stroke is generally believed to be preventable. The single bulb garlic is one of the local plants known as the medicinal plant in Indonesia. Garlic contains alliin compounds as antiatherosclerotic agent. This study aimed to determine the potential of active compound of single bulb garlic as an antioxidant that improve endothelial function by increasing nitric oxide (NO) to increase vasodilation. The bioinformatics webserver used in this study are: Pubchem, Pharmmapper, Swiss Target Prediction, Superpred, Uniprot, and Protein Data Bank. Docking software using PyRx, PyMOL and Discovery Studio. Based on these steps, it was found that alliin interacts with nitric oxide synthase (NOS) through hydrogen and alkyl bonds. Alliin is effective as an inhibitor of NOS based on binding afinity (-5.4 kcal / mol) although no more negative than an aspirin inhibitor based on binding afinity (-6.5 kcal / mol). Based on the docking results, it is found that alliin is effective as a potential drug to decreased atherosclerosis
On Designing Interactive Online Atlas of Reptile Anatomy (Mabouya multifacsiata)
Get PDFThis research is an integration between fields of Biology, Photography, Design, and Informatics Engineering. The study aimed to build an interactive online atlas of reptile anatomy to improve the accessibility and data sharing (free access) of reptile anatomy. Website was developed using SDLC (System Development Life Cycle) which consist of five steps as follows: website’s strategic planning, determine the scope of website, website’s requirements analysis, design and implementations of website, and testing. Based on the results of testing and system implementation, it can be concluded that online interactive atlas (AtlasAnatomy.org) had been successfully built as anatomical educational media of reptile