Biology, Medicine, & Natural Product Chemistry (E-Journal - Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity)
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Antioxidant Activities of the Leaf Extract and Fractions of Dryopteris filix-mas (L.) Schott could be Attributed to The Abundance of Polyphenol Compounds
Get PDFDryopteris filix mas (D filix-mas) is wildly used in ethnomedicine for the management of rheumatoid arthritis, wounds and other diseases. We investigated the anti-oxidant activities of its leaf extract, and chromatographic fractions. The ethanol leaf extract was partitioned into four fractions; n-hexane, ethyl acetate, n-butanol and water. Ferric reducing anti-oxidant power (FRAP), 1, 1-diphenyl-2-picrylhydrazil (DPPH) and nitric oxide (NO) scavenging in vitro assays were carried out on the extract and fractions at 6.25, 12.5, 25, 50, 100, 200, 400 and 800 µg/mL. The most active fraction (ethyl acetate fraction) was further purified using chromatographic techniques to isolate its major compound whose structure was elucidated using ID nuclear magnetic resonance (NMR) and mass spectrometry. The ethyl acetate fraction produced the highest free radical scavenging activity among the other fractions. The fraction (VLC-E7) from which the bioactive compound, quercetin-3-O-αL-rhamnopyranoside, was isolated had the best FRAP and DPPH scavenging activities with EC50 and IC50 values of 88.81 ± 3.41 and 26.87 ± 0.24 respectively more than the ethyl acetate fraction. This study revealed that the polyphenol flavonoid, quercetin-3-O-αL-rhamnopyranoside could be responsible for antioxidant activity of ethno-medicinal property of D filix-mas leaf
A Mathematical Model of the Covid-19 Cases in Indonesia (Under and Without Lockdown Enforcement)
Get PDFCOVID-19 stands for Corona (CO), Virus (VI), Disease (D) and year 2019 (19), which is COVID-19 first appeared in 2019. Mathematical model of covid deployment in Indonesia under and without lockdown case uses the SIRV model, such as Susceptible, Infected, Recovery, and Virus. The results of this model state that under lockdown the spread of COVID-19 could be stopped. If it were not under lockdown it can multiply 1,276 times higher over two months
Toxicological Effects of Ethanolic Stem Bark Extract of Xylopia Aethiopica on Testicular Oxidative Stress Markers and Histology of Male Rats
Get PDFImpairment in testicular function can occur through perturbations in testicular oxidative stress markers and histology. Xylopia aethiopica (XE) is used to enhance fertility in males, but with information gap on its effect on testicular oxidative stress markers and histology. The present study assessed the effects of ethanolic stem bark extract of Xylopia aethiopica (EEXA) on testicular oxidative stress markers and histology of male albino rats. Sixty adult male albino rats (200g-250g) were randomly grouped into 4 (A-D) of 15 rats per group. The rats in the control group A (A1-A3) were administered per oral (p.o) with water (0.2 mL/day) for 15, 30 and 60 days respectively. The rats in groups B (B1-B3), C(C1-C3) and D (D1-D3) were administered p.o with EEXA (200, 400 and 800 mg/kg/day) for 15, 30 and 60 days respectively. The rats were anesthetized at the termination of EEXA administration and were dissected and testes removed. The testes were weighed and evaluated for oxidative stress markers and histology. Testicular weights were decreased in a dose and-time dependent fashion in EEXA-treated rats. Significant decreases in testicular superoxide dismutase, glutathione, catalase, and glutathione peroxidase levels with significant increases in malondialdehyde levels in a dose and time-dependent fashion were observed in rats administered with EEXA. Testicular histology showed cellular necrosis, degeneration and loss of interstitial tissues in rats administered with EEXA. This study observed that EEXA perturbed testicular oxidative markers and histology. Its use may impair testicular function
The Potential of Chrysin of Oroxylum indicum L. to Induce Carbonic Anhydrase (CA) to Improve Cattle Fertility
Get PDFThe Artificial Insemination (AI) aims to develop the potential of the cattle reproduction comprehensively. The success of AI results is highly dependent on the level of livestock fertility. Therefore, improv the quality and quantity of semen could be done by hormone induction. However, hormone prices are expensive. This problem could be overcome using alternative way with the natural compound containing chrysin which might has the same function. The purpose of the study was analyze the potential of chrysin compound from Bungli plant (Oroxylum indicum) as fertilizer of males through bioinformatics approach. Methods performed through data search by webserver with the following stages: (1) Pubchem, (2) Swiss (3) Target Prediction, (4) Uniprot, (5) Protein Data Bank and (6) docking software using PyRx, PyMOL and Discovery Studio. The results showed that the chrysin compound interact with carbonic anhydrase (CA) expressed from the testes of the cattle, through van der Waals and Pi-Alkyl bonds. Chrysin is effective as CA ligand inducers based on affinity bonds (-8.4 kcal/mole) and more negatively than flavonol as a control compound with binding affinity (-6.2 kcal/mol). This suggests that chrysin is an effective compound as a potential drug for improved livestock fertility
A Chemical Overview of Azanza garckeana
Get PDFAzanza garckeana is a popular fruit tree in Nigeria, specifically in Gombe State, where it is locally called ‘goron tula’ which means ‘Kola of Tula’. It is also found in part of some African countries. Different plant part of this small tree/shrub has recorded different uses by the locals; uses ranging from its fruits being edible and others parts helping to remedy different diseases, especially sexually related diseases. It also records use as booster for sexual performance. The uses of the plant are majorly attributed to the presence of chemicals. Its local use initiates the necessity of this review to enhance the research for drug discovery since Chemicals are the chief constituencies responsible for its medicinal importance
Cover, Editorial Board, Guidance for Authors, & Table of Contents | Vol. 9 No. 1 April 2020
No full textAnatomical and Histological Study of Shark (Carcharhinus sorrah) Kidney
Get PDFSharks are sea water fishes belong to the class Chondrichthyes, Subclass Elasmobranchii. Sharks are cartilaginous fish that have a different osmoregulation process than any other sea water fish. Cartilaginous fish is the only vertebrate that can maintain urea. This study aims to determine the anatomical and histological structure of the kidney in the anterior, medial and posterior parts of kidney. The study was conducted by observing anatomy of the kidney. Histological preparations were made using the paraffin method. Qualitative descriptive data analysis was done. Research results show that shark kidneys consist of three parts, namely the head kidney, the body kidney, and the tail kidney. Kidney sharks are brownish red with a size of 18 cm long. Histological observations of shark kidney in the head kidney reveals many glomerulus, body kidney reveals many distal and tubule proximal contractile tubules whereas tail kidney reveals stroma that is rarely found in vertebrate kidney
Novel Approaches for Detection Fluorescent-Labeled by Cellvizio Lab System on Hippocampal CA1 Region
Get PDFNeurosteroids have been identified in the 1981. Dehydroepiandrosterone sulphate (DHEAS) is one of the vital neurosteroids that de novo synthesized in the nervous system from cholesterol precursor (Baulieu & Robel, 1998). The aim of the study is to develop a method for fluorescence labelling. Alexa Fluor 488 dye with DHEAS antibody can binds the DHEAS antibody in the rat brain monitored by Cellvizio Lab System. DHEAS antibody (IgG isotype antibodies) was fluorescently conjugated by an amine-reactive compound, Alexa Fluor 5-SDP ester 488 dye. The resultant Alexa Fluor 488-conjugated antibodies were collected and analyzed by UV-Vis spectrophotometer instrument. The absorbance of the protein-dye conjugate at 280 nm and 494 nm were measured. Then, the degree of labeling (DOL) was calculated to achieve the desired results. Fluorescence labelling were carried out into the CA1 region of hippocampus Sprague-Dawley rat. We reported that the conjugation was successful. Optimal labeling depending on degree of labeling (DOL) needs some necessity to achieve and effective binding to the target neurosteroid, DHEAS. Cellvizio Lab system connected with Fiber Fluorescence Microscopy (FFM) probe is presented as a new approach in real-time imaging of DHEAS. In conclusion, we have developed a new method of DHEAS-Alexa Fluor fluorescence labelling to visualize and evaluate the changes of DHEAS fluorescence level in the rat hippocampus. This novel approach as a diagnostic tool and can be used to better understand the mechanisms and functions of DHEAS and other neurosteroids in future research
Hepatotoxic Assessment of Tramadol-Diclofenac Use: A Study in a Rat Model
Get PDFThe concurrent use of tramadol and diclofenac may increase hepatotoxic risk due to their individual hepatotoxic effects. This study assessed the hepatotoxic effect of tramadol-diclofenac administration in albino rats. Twenty-four adult male albino rats (200-220g) randomized into four groups were orally administered with tramadol (12mg/kg/day), diclofenac (6mg/kg/day) and tramadol-diclofenac for 14 days respectively. The rats were anesthetized, blood samples were collected and evaluated for serum liver function and lipid parameters. Liver samples were weighed and evaluated for biochemical parameters and histology. The effects of tramadol-diclofenac on the body and liver weights did not differ significantly (p>0.05) when compared to control. Also, effects were not significant (p>0.05) on blood glucose, and serum cholesterol, triglyceride, low and high density lipoprotein cholesterol levels when compared to control. Liver and serum levels of aminotransferases, alkaline phosphatase, lactate dehydrogenase, gamma–glutamyl transferase, conjugated bilirubin and total bilirubin increased significantly in rats treated with tramadol (p<0.05), diclofenac (p<0.01) and tramadol-diclofenac (p<0.001) when compared to control. Furthermore, significant decreases in liver catalase, glutathione, superoxide dismutase, glutathione peroxidase levels with significant increases in malondialdehyde levels occurred in rats treated with tramadol (p<0.05), diclofenac (p<0.01) and tramadol-diclofenac (p<0.001) when compared to control. Hepatocyte necrosis was observed in rats treated with tramadol-diclofenac. Tramadol-diclofenac may increase hepatotoxic risk at doses used for this study