Biology, Medicine, & Natural Product Chemistry (E-Journal - Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity)
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Hippocratea africana Ethanol Root Extract and Fractions Attenuate Doxorubicin-Induced Testicular Toxicity and Oxidative Stress
Get PDFHippocratea africana root, used locally in treating poisoning, was investigated to confirm its antidotal potential in rats. The root extract (200-600 mg/kg) and fractions; dichloromethane (DCM) and aqueous, 400 mg/kg) were evaluated for hepatoprotective activity against doxorubicin-induced testicular toxicity in male rats. Testosterone level, lipid profile indices, testicular oxidative stress markers, and testis histology were used to assess the testicular protective effect of the extract. The root extract (200-600 mg/kg) and fractions, dichloromethane (DCM) and aqueous, 400 mg/kg reduced the serum levels of total cholesterol, triglycerides, LDL, and VLDL that were elevated by doxorubicin. In contrast, the high-density lipoprotein (HDL) reduced by doxorubicin was increased by the extract and fractions co-administration. Testosterone level, which was significantly (p<0.05) reduced by doxorubicin, was significantly (p<0.05-0.01) elevated by the root extract and fractions co-administration. The levels of GSH, GST, SOD, GPx, and CAT that were decreased by doxorubicin were significantly (p<0.01) elevated, and raised MDA level was reduced by the root extract and fractions. Histology of the testes sections of extract/fractions -treated animals showed absent/or reductions in the pathological features compared to the organotoxic-treated animals. The chemical pathological changes were consistent with histopathological observations, suggesting marked testicular protective potential. The anti-toxic effect of this plant may in part be mediated through the chemical constituents of the plant. The plant, H. africana possesses anti-toxicant properties which can be exploited in the treatment of doxorubicin-related toxicities
The Antibacterial and Antibiofilm Efficacy of Citrullus lanatus Rind Ethanol Extract Against Aggregatibacter actinomycetemcomitans
Get PDFWatermelon (Citrullus lanatus) rind contains secondary metabolite compounds which exhibit antimicrobial effects against Gram-positive and Gram-negative bacteria. Aggregatibacter actinomycetemcomitans can cause tissue damage and bone resorption, leading to aggressive periodontitis. Chlorhexidine as golden standard antimicrobial mouthwash can cause tooth staining. There is a need for herbal remedies with fewer side effects as an alternative treatment for periodontitis. This study aims to determine the antibacterial and antibiofilm efficacy of C. lanatus rind ethanol extract against A. actinomycetemcomitans. This is an experimental laboratory in vitro with a post-test-only control group design. The antibacterial test was conducted using the plate count method and the antibiofilm test using the microtiter plate biofilm assay method. The samples used were ethanol extract of C. lanatus rind 3.125%, 6.25%, 12.5%, 25%, 50%, and 100%, chlorhexidine 0.2% as positive control, distilled water, and BHI-B as negative control. As a result, extract concentrations from 12.5% to 100% exhibited antibacterial effects on A. actinomycetemcomitans equivalent to chlorhexidine. During the 3-hour incubation period, extract at 25% and 100% concentrations on all incubation times showed a better antibiofilm effect than chlorhexidine. It can be concluded that ethanol extract of C. lanatus rind had the potential as an alternative antibiofilm agent against A. actinomycetemcomitans
Identification of Marbofloxacin-Susceptible Bacterial Uropathogens in Feline Urinary Tract Infection of Different Age Groups
Get PDFUrinary tract infections (UTI), an adhesion and multiplication of bacterial uropathogens in the urogenital system, are one of the most important indications for antimicrobial use in veterinary medicine and contribute to the development of antimicrobial resistance. Previously, it was revealed that older cats with an average of 9.1 years old are most likely to develop UTI, however a contradicting investigation stated kittens and young adults are more likely to contract UTI. Along with the uprising of amoxicillin- and ampicillin-resistant uropathogens, the study aims to turn towards marbofloxacin reserve and to analyze their potency. Furthermore, the current study aims to enumerate and compare the number of uropathogens between age groups. Enumeration and identification of uropathgoens from urine samples of feline UTI patients were was conducted through the urine culture on EMB, MSA, and BHI medium followed by an IMViC test and inoculation to the Mueller-Hinton agar to observe the zone of inhibition. The investigation revealed that a higher number of younger cats and kittens were reported to have UTI and catheter-induced UTI with a higher number of uropathogens in the population. The uropathogens were further identified as Staphylococcus spp., Enterococcus spp., Enterobacter spp., Escherichia spp., and Klebsiella spp. Additionally, marbofloxacin was found to be effective in eliminating and inhibiting the growth of these uropathogens. In conclusion, younger cats were observed to be more prone to UTI of a diverse range of uropathogens, however, marbofloxacin may aid in the treatment of the cats as it has been proven to be most effective
The Effect of Paracetamol on The Development of Chicken Embryos
Get PDFParacetamol (N-Acetylaminophenol) is the most widely used over-the-counter drug in the world. Although considered safe for pregnant mothers, there is a concern regarding the teratogenic effect of paracetamol. This study aims to observe the teratogenic effect of paracetamol developing embryos. Using chicken embryos, the embryos were incubated for 48 hours and then injected with paracetamol in 3 concentrations, namely 10 ppm, 15 ppm, and 20 ppm. The control group and treatment group consisted of 3 replicates of fertile embryos. Then, the embryos were incubated again for 48 hours in the incubator. Data analysis was carried out qualitatively by observing any developmental alterations. Results showed that paracetamol caused enlargement of the head and heart edema. Exposure to paracetamol at concentrations of 15 ppm and 20 ppm affects the morphology of chicken embryos, especially the formation of the head and disrupts the process of angiogenesis and normal heart formation, causing bleeding and edema at higher concentrations
Comparison of Interleukin-1b-511 C/T Polymorphism in Schizophrenia of Batak and Minangkabau Ethnicities in Pematang Siantar
Get PDFThis research is an overview of a comparative study on the Interleukin-1β-511 C/T polymorphism in individuals with schizophrenia of Batak and Minangkabau ethnicities in Pematang Siantar, Indonesia. The study aims to investigate genetic differences among these ethnic groups to better understand susceptibility to schizophrenia, which is influenced by multiple factors including genetics. The research uses a comparative categorical analytic approach with a cross-sectional method and involves DNA isolation and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) examinations. Based on the results, significant demographic differences between the Batak and Minangkabau ethnic groups, particularly in gender distribution, age, onset of illness, duration of illness, and PANSS scores. Notably, it finds a significant difference in the occurrence of the C allele and the T allele between the two ethnic groups, with the C allele being more prevalent in the Minangkabau group and the T allele more so in the Batak group. This suggests that the IL-1β -511 C/T polymorphism may play a differential role in the susceptibility to schizophrenia among these ethnicities, indicating a potential for ethnic-specific risk factors or protective elements
Antiepileptic Potential of Justicia insularis Leaf Extract in Swiss Mice
Get PDFEpilepsy is a prevalent neurological condition characterized by repetitive seizures, necessitating the exploration of efficacious treatments. Justicia insularis, a plant with a historical use in treating convulsions and epilepsy, has garnered attention as a potential antiepileptic agent. This study aimed to investigate the antiepileptic properties of Justicia insularis leaf extract, focusing on generated seizure models in mice. The analysis of variance (ANOVA), specifically one-way ANOVA, was utilized to assess the statistical significance of the data. Seizure models were employed to evaluate the antiepileptic effects of Justicia insularis leaf extract. The p-values obtained from the one-way ANOVA were significant, with values of ap<0.005, bp<0.01, and cp<0.001. These results suggest the statistical significance of the observed effects in the seizure models. This study provides valuable insights into the therapeutic potential of Justicia insularis leaf extract as a treatment for epilepsy. The significant findings underscore the importance of further research into the use of natural medicines for managing epilepsy, potentially offering new avenues for treatment
Physicochemical, Antimicrobial, Lethality and In Vitro Antioxidant Profiles of Johnu Tisane: A Coffee (Coffea Arabica) Leaf Decoction Formula
Get PDFCoffee leaves-based tea has relevance in ethno-medications due to its rich phyto-constituents-related diverse pharmacologic activities. Jonhu tisane, a typical Coffea arabica leaf decoction has no supporting scientific basis amidst reported location, processing and specie-related variations. This investigated physicochemical, antimicrobial, lethality and in-vitro antioxidant profiles of Johnu tisane by acceptable methods. Results recorded moisture (92.26 %), ash (0.65 %), unsaponified matter (1.46 mg/100 g), free fatty acid (0.56 mgKOH/g), acid value (1.12 mgKOH/g), potential hydrogen (6.85), lethal concentration (1000 ppm) and concentration-dependent antimicrobial activity. Anti-oxidation results revealed total antioxidant capacity, ferric reducing antioxidant power, nitric oxide, hydrogen peroxide and 2, 2-diphynyl-1-picrylhydrazyl scavenging activities increased concentration-dependently compared to standard. Thus, Johnu tisane demonstrated low minerals and keeping quality; requisite physicochemical mix for consumption and bioactivity; high safety margin; antimicrobial potency; and requisite anti-oxidation capacity for in-vivo antioxidant role. These provided scientific support for its ethno-medicinal uses. They underscored the need to elucidate its pharmacologically active compounds; mechanistic roles in animal models; and the impact of ash to moisture mix variation on potential hydrogen, microbial and antimicrobial activities in relation to shelf life, bioactivity and in vivo anti-oxidative roles for novel insights on preserving sample quality, safety, bioactivity and in-vivo anti-oxidative outcomes
The Effect of Tapioca Flour and Bogor Taro Flour (Colocasia esculenta L. Schott) Formulations on the Chemical, Physical, and Sensory Characteristics of Catfish Sausage (Pangasius hypophtalmus)
Get PDFThis study was aimed to determine the effect of tapioca flour and Bogor taro flour formulation on the chemical, physical, and sensory characteristics of catfish sausage and to determine the best formulation. This study was arranged in a Randomized Complete Group Design (RCGD) with a single factor of tapioca flour and Bogor taro flour formulation with 6 treatments P1 (100%:0%), P2 (80%:20%), P3 (60%:40%), P4 (40%:60%), P5 (20%:80%), and P6 (0%:100%) with 4 replications. Data were tested for equality of variance with Barlett's test and data saturation with Tuckey's test. The data were analyzed for variance to determine the effect between treatments, then further analyzed using the HSD test at the 5% level. The results showed that the formulation of tapioca flour and Bogor taro flour had a very significant on water content, ash content, hardness, springiness, cohesiveness, appearance, and overall acceptance but no effect on the taste and aroma of catfish sausage. P5 was the best treatment with a water content of 52.08%, ash content of 1.55%, hardness of 270.75 gf, springiness of 10.03 mm, cohesiveness of 0.83, appearance of 2.93 (slightly dull), taste of 3.87 (typical of fish), aroma of 3.87 (typical of fish), overall acceptance of 3.88 (like), protein content of 15.61%, and fat content of 4.29%
Potential of California Variety Papaya Leaf Extract (Carica papaya L. California variety) As Bioherbicide of Snake Grass Weed (Cyperus rotundus L.)
Get PDFEfforts to increase production yields are by controlling weeds. Weeds that often grow and we encounter in cultivated plants are weed grass (C. rotundus). California papaya leaves have the potential to be a bioherbicide because they contain allelochemicals that can inhibit weed growth. This study aims to determine the effect of papaya leaves (C. papaya) on the growth of teki grass (C. rotundus) and determinetheconcentration level of papaya leaf allelopathy that most effectively inhibits the growth of teki grass.The method used was a completely randomized design (CRD) with five replications and four treatmentsof0%, 30%, 50%, and 70%. For data analysis in this study using the SPSS version 25.0 program. Papaya leaf extract (C. papaya) is able to inhibit the growth of weed grass (C. rotundus). The concentration of papaya leaf extract (C. papaya) that most effectively inhibits the growth of weed is 50% concentration with a mortality rate (64%), for the lowest plant heightis50% concentration which is 13.84 cm, for the slowestgrowthrate is 50% concentrationwith a growthrate of -0.16 cm
Antibacterial Activity Testing Propionibacterium acnes Combination of 70% Ethanol Extract of Starfruit Leaves (Averrhoa bilimbi L.) and Jarak Pagar Leaves (Jatropha curcas L.)
Get PDFAcne is an inflammation accompanied by blockage of oil gland ducts that gather and cause blackheads. The onset of acne can be caused by the bacterium Propionibacterium acnes. Flavonoid compounds and tannins contained in star fruit leaves and jatropha leaves can be used as antibacterials. Both extracts from the plant can be combined to achieve antibacterial effects that can be used as an alternative to acne treatment. The purpose of this study was to determine the antibacterial activity of star fruit leaf extract and jatropha leaf and determine the optimal concentration resulting from the antibacterial activity of the extract combination. The method used is experimental and antibacterial tests in vitro. The results of antibacterial tests on the combination of jatropha leaf extract and star fruit leaf extract against P. acnes had the most optimal average inhibitory zone of 9,642 mm with a ratio of 1:2 at a concentration of 10%