Biology, Medicine, & Natural Product Chemistry (E-Journal - Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity)
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Isolation and Characterisation of Essential oils of Mango (M. indica) Leave
Get PDFThis research work aimed at isolation and characterisation of essential oils of Mangifera. indica (M. indica) leaves. Phytochemical screening was carried out to determine the bioactive components present in the leaf sample as well as the antimicrobial activity of the crude ethanolic extract. The percentage yield of essential oils (colourless) of the leaf was 0.19%. The phytochemical screening revealed the presence of alkaloid, glycoside, flavonoid, saponin and tannin.  Steroids was absent in the extract. The antimicrobial evaluation of the essential oil of M. indica leaf revealed a significant activity (zone of inhibition). The percentage (%) of Diphenyl picryl hydrazine (DPPH) scavenged of antioxidant activities for the essential oils of M. indica at different concentration of 5µl, 10µl, 25µl and 100 µl showed a significant antioxidant activity. In concluded results, it’s clearly observed that, when the concentration of both ethanolic extract and essential oils leaf sample increases, the antimicrobial and antioxidant properties increases when compared to the standard (Amoxicillin) and that of essential oils has a significant property than other extracts. Considering the results of phytochemical screening, it’s also a potential source of cost-effective food supplements, nutritive ingredients and antibacterial agent for improving human health and curing acute and chronic diseases. The GC-MS analysis of the essential oils for the leaf sample revealed fifty two (52) different chemical compounds which include Alloaromadendrene, Humulene, Bicyclo[4,4,0]dec-1-ene, 2-isopropyl-5-methyl-9-methylene, Bicyclo[7.2.0]undec-4-ene,4,11,11,-trimethyl 8-methylene-,[1R-(1R*,4Z,9S*)]
Effectiveness Test of Methanol, Ethyl Acetate, and Chloroform Fractions of Bidara Leaf Extract (Zizyphus mauritiana L.) on Wound Healing in Rabbits (Oryctolagus cuniculus)
Get PDFBidara (Zizyphus mauritiana L.) contains alkaloids, glycosides, saponins, flavonoids, terpenoids, phenols, and the best antioxidant properties of its leaves. In bidara leaf, they are antibacterial, antiviral, and antiseptic, play a role in cell regeneration and repair, so they are effective in wound healing. This study aimed to determine the effectiveness of bidara leaf extract fractions on incision wounds and determine which fraction was more effective in narrowing the incision wound. Making wounds on rabbits (Oryctolagus cuniculus) using the Morton method, by making an incision on the back with a diameter of 2 cm. Testing the effectiveness of fractions in wound healing with rabbits divided into 5 groups, namely positive control, negative control, methanol fraction (MeOH), ethyl acetate fraction (Ethac), and chloroform fraction (CHCl3) with a dose of 100 mg/KgBB rabbit in 1 ml of solvent. The diameter of the wound area was measured using the Macbiophotonic Image J program, and the data were analyzed using One Way Anova (ANOVA) followed by the Tukey test. The results of data analysis using the ANOVA method with a significant value of 0,004 (p<0,05). The conclusion of the results of the research conducted for 8 days shows that the MeOH, Ethac, and CHCl3 fractions of bidara leaf extract have the effectiveness of wound healing in rabbits with differences in healing shown by the Ethac fraction with a better effect than the MeOH and CHCl3 fractions
Effect of Mixing Time Variation and Framing Time on the Quality of Coffee Grounds-based Solid Soap
Get PDFThe increase in public consumption of coffee has resulted in an increase in wasted coffee grounds. Coffee grounds have good content for the skin, so they can be reused as a basic ingredient in soap-making. In the soap making process, the mixing and framing stages can affect the quality of the soap. This study aims to determine the effect of mixing time of 24.5 minutes, 25 minutes, and 25.5 minutes as well as framing time of 3 days, 5 days, and 7 days on the physical and chemical quality of solid soap made from coffee grounds. The physical quality parameters observed are colour, texture, cracking, and foam power. The chemical quality parameters are foam stability, pH, moisture content, and free alkali. The method used was the questionnaire method followed by statistical analysis using the Kruskal Wallis test for physical quality tests and the experimental method followed by statistical analysis using the Two-Way ANOVA test for chemical quality tests. The results showed that all variations in mixing time and framing time had an effect on the physical and chemical quality of solid soap and there were significant differences. The panellists assessed the physical quality of the soap to be attractive, hard, with no cracks, and foamy, while the results of the chemical quality test, the soap produced is safe to use by the quality standards of solid soap with a foam stability range of 82%-97%, pH 10.3-11.8, moisture content 6%-14%, and alkali free 0.03%-0.09%
Prospecting Marine Natural Products as the Disease-Modifying Treatment of Alzheimer’s Diseases
Get PDFAlzheimer's disease (AD) is a severe and high costs health problem all over the world. To date, there is no therapy capable of curing AD, making drug discovery a challenging and intriguing field to explore. Targeting marine natural products (MNPs) as a source of drug leads is a suitable choice considering the content of bioactive compounds and promising pharmacological benefits. This article aims to look at MNPs with prospects in several prime targets in AD pathology to cure AD. Marine vertebrates (fishes), marine invertebrates (sponges, tunicates, ascidian, clams, scallops, sand dollars, crabs, cockle, sea cucumber, shrimp, bryozoa, marine worm), algae/seaweed, and marine microorganisms (marine fungi, bacterium, dinoflagellata, microalgae) can be potentially used as disease-modifying treatments (DMTs) for AD. By targeting multiple aspects of AD pathology, these MNPs offer a multifaceted approach to treating and potentially modifying the disease course. This result is an intriguing gap for researchers in the discovery and development of new drugs that can improve AD pathology
Anti –Inflammatory Activity of Propolis Trigona sp. Water Extract from North Lombok with Red Blood Cell Membrane Stability Method
Get PDFPropolis is a bee product that can be used as an anti-inflammatory. Cultivation of propolis-producing bees is also carried out in North Lombok. However, propolis has not been utilized optimally by the people of North Lombok. Apart from that, testing of North Lombok propolis is still limited to the chemical content and antioxidant activity of propolis extracted with ethanol solvent. Therefore, this study aims to determine the activity and effective concentration of North Lombok propolis water extract as an anti-inflammatory using the red blood cell membrane stability method. Groups include control groups such as positive control (diclofenac sodium), negative control (distilled water), and test groups (propolis water extract concentrations of 10%, 12.5%, 15%, 17.5%, 20%, 22.5%, 25%, 27.5%, and 30%). In The first step human blood was centrifuged of human blood, and then part of the red blood cells (sediment) was taken and saline was added to obtain a red blood cell suspension. Next, mixing the test solution was carried out in the order of 0.5 ml of red blood cell suspension, then 0.5 ml of extract was added (0.1% w/v Na-diclofenac solution in the positive control, distilled water in the negative control), 1 mL of buffer solution and 2 mL of hyposaline solution, then homogenized. Each group was incubated at 37°C for 30 minutes, then centrifuged for 10 minutes at 3000 rpm. The supernatant was taken, and the absorbance was read with a UV-Vis spectrophotometer at 560 nm. Next, the percentage value of red blood cell hemolysis protection was calculated. The data obtained were tested statistically using One-way ANOVA and post-hoc (LSD) tests SPSS version 29. The results showed that propolis water extract concentrations were 10%, 12.5%, 15%, 17.5%, 20%, 22.5%, 25%, 27.5%, and 30% have anti-inflammatory activity because they can increase the stability of the red blood cell membrane with a percentage of hemolysis protection of respectively 57.92%, 59.99%, 60.99%, 61.99%, 64.31%, 69.59%, 75.07%, 79.77% and 84.45%. Propolis water extract concentrations of 10%, 12.5%, 15%, 17.5%, 20%, 22.5%, and 25% had anti-inflammatory effects that were not significantly different from the positive control (p>0.05). The 27.5 % and 30% concentrations had a higher percent hemolysis protection value than the positive control (p<0.05)
Chemical Constituents and Evaluation of Bioactivity of Vernonia hymenolepis A. Rich Root Extracts
Get PDFThe medicinal herb Vernonia hymenolepis has been used to cure a wide range of ailments such as cancer, gonorrhea, stomach ache, malaria, hepatitis, pneumonia, toothache, diarrhea, amoebiasis, typhoid, hypertension, and constipation by diverse societies in Ethiopia, Kenya, and Tanzania. However, its biological and phytochemical data is extremely insufficient. Thus, the aim of this research work was to study the chemical constituents and antimicrobial activity of the root part of this plant. The roots of plant were dried by air under shade and then successively extracted with chloroform and methanol. The chloroform root extract was subjected to silica gel column chromatographic separation which gave two compounds, namely; betulinic acid (1) and 2-hydroxy-3-(4-hydroxyphenyl)-2-propenoic acid (2). The structures of the isolated compounds were elucidated using 1H and 13C NMR spectroscopic techniques and by comparing with literature reports. The crude extracts were assayed in vitro employing disc diffusion technique against four bacterial strains (Bacillus subtilis ATCC11778, Staphylococcus aureus ATCC25923, Escherichia coli ATCC25922, and Pseudomonas aeruginosa ATCC27853) and one fungal strain (Candida albicans ATCC 10231). Significant antibacterial activity was demonstrated by the crude extracts; with the chloroform extract exhibiting greater activity against Escherichia coli (18 mm) when compared to gentamicin (25 mm). The methanol extract revealed the highest activity (13 mm) against Candida albicans with the reference drug, clotrimazole showing an inhibition zone of 12 mm. The antimicrobial activity exhibited by root extracts of V. hymenolepis corroborates its traditional use against bacterial diseases
Antibacterial Activity Screening of Bacillus sp. AM12 Associated with Mangrove Soil
Get PDFThe use of synthetic antibiotics to treat bacterial infections can trigger resistance of pathogenic bacteria to antibiotics. One effort to overcome this is to explore microorganisms that produce antibacterial compounds from nature. A previous study reported isolates of Bacillus sp. AM12 from mangrove soil displayed antibacterial potential based on preliminary screening of the perpendicular streak test. This study was designed to confirm the antibacterial potential of Bacillus sp. AM12 uses chemical solvent extraction. Bacillus sp. AM12 was fermented in 100 mL liquid ISP-2 sterile and shaken at 150 rpm for 7 days. The supernatant was filtered from the cell mass using Whatman paper and extracted using 100 mL of ethyl acetate solvent (1:1, v/v) twice. The filtrate was evaporated at 40ºC until a thick, clear yellowish colored extract was obtained. The thick extract was tested for antibacterial activity using the Kirby-Bauer method against two Gram positive and two Gram negative bacterial targets. Antibacterial screening showed moderate diameter zone of inhibition of 6.72 ± 0.21 mm, 6.82 ± 0.15 mm, and 6.62 ± 0.21 mm against Staphylococcus aureus ATCC 25923, Streptococcus mutans FNCC 0405, and Klebsiella pneumoniae ATCC 70060, respectively. However, no antibacterial activity was observed against Escherichia coli ATCC 25922. Analysis of the chemical composition of ethyl acetate crude extracts using GC-MS has detected 10 different compounds, in which the 3 most dominant antibacterial compounds were Benzene, 1,2,4-Trimethyl-, Benzene, 1-ethyl-2-methyl- and 2-butoxyethyl acetate. In general, these results provide an initial description of Bacillus sp. AM12 is a potential of antibacterial producer
Phytochemical Screening and Antioxidant Activity Analysis of N-Hexane Extract of Sonneratia alba Mangrove Leaves
Get PDFMangrove plants have been widely used by people in various regions of Indonesia as traditional medicine for the treatment of wounds, fever, diarrhea, and others. Extreme habitat in coastal areas with high salinity and low oxygen level makes mangrove plants produce a variety of unique secondary metabolites and are rich in antioxidants. Although there have been studies on phytochemicals and antioxidant activity conducted, the study of mangroves in Bali, Indonesia, has not been widely explored. This study aims to determine the compounds contained in mangrove leaf extract and their antioxidant activity. The species to be studied is Sonneratia alba, which is commonly found in the Ngurah Rai Mangrove Forest, Bali, Indonesia. In this experimental study, compound extraction was carried out by maceration method using n-hexane solvent. Phytochemical screening using qualitative methods and GC-MS was carried out to determine the phytochemical compounds in the extract, while 1,1-diphenyl-2-picrylhydrazyl (DPPH) method was used to determine antioxidant activity. The result showed that the n-hexane S. alba mangrove leaves extract display strong antioxidant activity with IC50 value of 64.432 ± 7.675 ppm. The qualitative phytochemical tests showed that the extract contained phenol and steroid, which are known to have antioxidant properties. Moreover, GC-MS analysis showed that there are two compounds with the largest %area namely gamma-sitosterol and dl-alpha-tocopherol (52.88% and 7.77%, respectively), which have been reported to have antioxidant activities. To conclude, these findings demonstrate that n-hexane extract of S. alba mangrove leaves from Ngurah Rai Mangrove Forest, Bali, to have potential antioxidant activity
Physical Characteristics of Immobilized Cells Acetobacter xylinum of Various Concentrations of Na-alginate
Get PDFImmobilization of Acetobacter xylinum cells by trapping technique is considered one way to maintain cell viability and can be used repeatedly. The purpose of this study was to determine the physical characteristics of immobilized cells in different concentrations of Na-alginate and apply them to the manufacture of nata de coco, as well as to see the surface and pore shape of immobilized cells after fermentation. This study used a descriptive method with visual observation. This study used one factor, namely various concentrations of Na-alginate, namely 2%, 3%, 4%, and 5% w/v. The immobilized cells applied to the manufacture of nata de coco had only a Na-alginate concentration of 3% resulting in a nata fiber layer close to that of a control. Immobilized cells with a Na-alginate concentration of 2% have a cracked, slightly pore surface with a size of 60.37 μm, while a Na-alginate concentration of 5%, have a smooth surface, many pores, with a size of 10.55 μm. Thus, the concentration affects the characteristics of immobilized cells. Application of immobilized cells with a Na-alginate concentration of 3% produces nata de coco with a fairly thick and stable fiber layer characteristic (close to control)
Genotoxic and Cytotoxic Activities of Cornhusk Extract of Zea mays and Leaf Extract of Sacharum officinarum
Get PDFZea mays husk and Saccharum officinarum have been used for years in ethnomedicine for their antimalarial, anti-inflammatory, antipyretic, antidiabetic, and antiphlogistic activities. The cytotoxic and genotoxic effects of Zea mays husk and Saccharum officinarum leaf extracts on the root meristem cells of Allium cepa were investigated. Onion bulbs were exposed to 2.5 mg/ml, 5mg/ml, and 10 mg/ml concentrations of the extracts for macroscopic and microscopic analysis. Tap water was used as a negative control and Methotrexate (0.1 mg/ml) was used as a positive control. There was statistically significant (p < 0.05) inhibition of root growth depending on concentration by the extracts when compared with the negative control group. All the tested extracts were observed to have cytotoxic effects on cell division in A. cepa. The extract induced chromosomal aberrations and micronuclei (MNC) formations in A. cepa root tip cells were significant (p<0.05) when compared with the control group. The extracts treatment further induced cell death, ghost cells, cells membrane damage, and binucleated cells. The Zea mays husk extract was found to exhibit higher cytotoxic and genotoxic potential than Saccharum officinarum leaf extract. These results suggest that Zea mays husk and Saccharum officinarum leaf extracts possess cytotoxic and genotoxic effects on A. cepa