Centro de Investigación y Tecnología Agroalimentaria de Aragón
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Physiological and biochemical parameters controlling waterlogging stress tolerance in Prunus before and after drainage
Full text linkWaterlogging is associated with poor soil drainage. As a consequence oxygen levels decrease in the root environment inducing root asphyxia and affecting plant growth. Some plants can survive under these conditions triggering complex anatomical and biochemical adaptations, mostly in the roots. Long- and short-term responses to waterlogging stress were compared in two trials using a set of two myrobalans (Prunus cerasifera Erhr), P.2175 and P.2980, as tolerant rootstocks and two almond x peach [Prunus amygdalus Batsch x Prunus persica (L.) Batsch] interspecific hybrids, Garnem and Felinem, as sensitive ones in two consecutive years. Stomatal conductance and chlorophyll content were measured in the long-term trials to assess survival performance, while the enzyme activities of superoxide dismutase (SOD, EC 1.15.1.1), guaiacol peroxidase (POD, EC 1.11.1.7), and catalase (CAT, EC 1.11.1.6) were measured in the short-term trials to study early antioxidant response. The incidence of the stress in the root environment was different as a result of the different plant development at the moment of the treatment, as a consequence of different environmental conditions both before and during the treatment between the 2 years. The activity of the different enzymes was higher in the sensitive genotype Felinem than in the tolerant P.2175. This result shows an activation of the antioxidant system and has been observed to depend of the different nature of the roots between the 2 years. As the antioxidant enzymes seem to work more efficiently when roots are more aerated, we cannot conclude that they are responsible for the higher tolerance observed in the myrobalan plums.This work was supported by RTA- 08-0086 and A12 research group from the Government of Aragon.Publishe
¿Cómo afecta una restricción nutricional a corto plazo sobre los rendimientos y el metabolismo de las vacas nodrizas a lo largo de la lactación?
Full text linkLos cambios de dieta en un corto espacio de tiempo indujeron adaptaciones tanto productivas como metabólicas en vacas de carne en lactación. Los resultados de esta investigación muestran que las vacas de carne utilizan diferentes estrategias metabólicas (y con distinto éxito) para hacer frente a las perturbaciones nutricionales en función de la fase de lactaciónAl personal técnico de la Finca Experimental La Garcipollera y el CITA.
Financiación de la Unión Europea (GenTORE, H2020 contrato 727213)
e INIA-FEDER (RZP2015-01), y contrato predoctoral de K. Orquera del
Gobierno de AragónPublishe
Early pregnancy diagnosis in sheep using near-infrared spectroscopy on blood plasma
Full text linkThe objective of this study was to evaluate the ability of near-infrared reflectance spectroscopy (NIRS) to discriminate between pregnant and nonpregnant ewes in early stages of pregnancy after artificial insemination (AI) from blood plasma. Samples were collected using jugular puncture at 18 and 25 days after AI from 188 Rasa Aragonesa and Ansotana ewes. Plasma samples were analyzed for pregnancy-associated glycoprotein (PAG) and progesterone (P4) using ELISA commercial kits. The spectra of plasma samples were recorded in the visible and near-infrared ranges. The performance of these tests were compared, using as criterion standard the pregnancy status determined using transabdominal ultrasonography at 45 days after AI. Pregnancy rate was 47.9% (90/188). At Day 18, sensitivity was similar in NIRS and P4 tests (98.9% vs. 100%; not significant) and greater than PAG (32.2%; both P < 0.001). Specificity was similar in NIRS and PAG tests (both 100%) and greater than that of P4 (84.7%; P < 0.001). At Day 25, sensitivity and specificity of NIRS and PAG were both 100%. It can be concluded that NIRS was an accurate method of diagnosis of pregnancy at Days 18 and 25 after AI in ewes. (C) 2014 Elsevier Inc. All rights reserved.This study was funded by the Government of Aragon
(Research Group on Improvement of Sheep Production).
ELISA kits for pregnancy-associated glycoprotein determination were given by Prof. J.F. Beckers, Université de Liège. The authors thank Pilar Sánchez and María Ángeles Legua for their technical collaboration. An Spanish application patent related to the present research “Procedimiento de identificación de animales gestantes” with application number P201331294 has been filed the 2nd
September of 2013Early pregnancy diagnosisEweFertilityNIRSPAGProgesteroneSubmitte
Effect of nanocomposite packaging containing different proportions of ZnO and Ag on chicken breast meat quality
Full text linkThis study reports the antimicrobial capacity, nanoparticle migration properties and the influence on some meat quality traits of a packaging based on a low density polyethylene (LDPE) blended with a nano-antimicrobial master batch composed of Ag and ZnO (5% and 10% w/w). Meat was aged for storage times of 0, 7, 10, 15 or 21 days. Composition of the package atmosphere, some microbiological analyses, meat sensorial quality, meat color, visual appearance score and lipid oxidation index (TBAR) values were determined. Irrespective of the packaging, the O-2 concentration decreased and the CO2 concentration and count for all types of microbe increased with storage time. Redness, yellowness and Chroma of the breast meat increased until to 7 days whereas maximum lightness values were found at 15 days. Visual appearance scores decreased and lipid oxidation increased with storage time. It was found that adding ZnO + Ag nanoparticles to LDPE packaging has an antimicrobial effect whilst migration amounts were well within those allowed by law. (C) 2013 Elsevier Ltd. All rights reserved.This study was funded by project IPT-060000-2010-019. We thank the sensorial panelists for their valuable help.Publishe
Red AgriFoodTe Red de conocimiento e innovación agroalimentaria (AKIS) de Teruel
Full text linkActuación subvencionada por el Gobierno de España y el Gobierno de Aragón con cargo al Fondo de Inversiones de Terue
Development of an innovative intravaginal model of probiotic inoculation in dairy ovine flocks: positive effects on the vaginal microbiota, vaginitis and fertility.
Full text linkIn vitro studies have reported the antimicrobial effect of an inoculum (L2) made from commercial probiotic strains of Lactobacillus spp. (Lactobacillus (L.) crispatus, L. brevis and L. gasseri) against some ruminants' pathogens. However, their possible beneficial effects on controlling the vaginosis caused by the use of intravaginal sponges for oestrus synchronisation have not been evaluated in in vivo models. Therefore, the purpose of this study was to perform an intravaginal double inoculation of this same non-lyophilised (non-freeze-dried) L2 inoculum, in 120 ewes from four dairy commercial ovine flocks using straws similar to those used in artificial insemination. Globally, in the entire study population, the fertility percentage (65%) of the inoculated groups increased by 10% compared to the control groups (55%). Specifically, increases in the percentages of pregnant sheep in flocks 1 and 4 (10.7 and 5.7% respectively; P > 0.05) and flock 3 (27.3%; P < 0.05) were observed in inoculated ewes. Overall, the administration of L2 on the day of intravaginal sponge insertion decreased the number of ewes with vaginitis by 11.13% at the time of sponge removal (T1). In the first flock, the administration of the probiotic positively modulated the microbiota with an increased richness and presence of Lactobacillales accompanied by a reduction in Mycoplasma spp. Genera such as Fusobacterium, Streptobacillus, Campylobacter or Trueperella were linked to the presence of vaginitis at T1. Genera belonging to Lactobacillales, including some genera previously reported to be beneficial for pregnancy, significantly increased at the time of pregnancy diagnosis but significantly decreased at T1. The results of this study suggest that the use of probiotics via intravaginal inoculation as a strategy to positively modulate the microbiota and vaginal inflammation, derived from the use of intravaginal sponges, is also associated with improved fertility. This novel approach could improve animal welfare in oestrus-synchronisation protocols and represents an alternative to the prophylactic use of antibiotics.We would like to thank the livestock associations AGRAMA,
ASSAFE and CONFELAC, but also Neiker (Basque Institute for Agricultural Research and Development) and OVIGEN (centre for selection and genetic improvement of sheep and goats of Castilla y
León) for their participation in this study.Alternative to antibiotic therapyAnimalwelfareEwesLactobacillusMollicutesPublishe
Mapping the genomic landscape of Prunus spp. with PrunusMap
Full text linkNext-generation sequencing has fueled significant advancement in plant breeding tools, such as genome-wide association studies and single-nucleotide polymorphism (SNP) analysis. In this dynamic landscape, plant databases housing SNP markers have evolved into hubs facilitating breeding initiatives and genomic research. PrunusMap, accessible at https://prunusmap.eead.csic.es is an open-source Web application tailored for the Prunus community. Featuring a user-friendly interface, PrunusMap empowers users to seamlessly align and locate markers across multiple genome versions of Prunus species and cultivars, supporting different queries and formats. Beyond locating marker positions, it provides a comprehensive list of annotated nearby genes and proteins. This streamlined process, driven by four intuitive features ‘Find markers’, ‘Align sequences’, ‘Align proteins’, and ‘Locate by position’, significantly reduces workload and boosts efficiency, particularly for users with limited bioinformatics expertise. Moreover, PrunusMap’s versatility is underscored by its commitment to incorporate additional Prunus genome sequences, annotations, and markers upon user request.The authors would like to thank Dr Veronica Guajardo for
providing data on ‘Adafuel’ SNPs and Bixente Sehabiague for
his technical assistance. This work was funded by CSIC [grants
2020AEP119 & FAS2022_052], the Spanish Research Agency
[grants AGL2017-83358-R & PID2023-146749OB-I00, funded by
MCIN/AEI/10.13039/501100011033 ‘A way of making Europe’] and
the Government of Aragón [grants A44, A09_23R, A10_23R, and
PhD contract to Najla Ksouri 2018–2023], which were cofinanced
with FEDER fundsPublishe
Streptococcus suis transfiriere genes de resistencia a antibióticos a otros estreptococos patógenos de humanos
No full textEste estudio fue financiado por los proyectos ABC-VaccineSs (MCIN/AEI/10.13039/501100011033) concedido por la Agencia Estatal de Investigación y TRANSIT (Ref. LMP58_21) de I+D+i de la Dirección General de Aragón