Journal of Tropical Crop Science
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Plant Growth Performance of Top Grafted Young Cacao at Various Elevations in Indonesia
Get PDFThe purpose of this study was to evaluate the plant growth performance of top grafted young cacao cultivated in various elevations. This research was conducted from January-August 2019 in South Sulawesi and Central Sulawesi. A total of 54 plants were taken randomly from 6 locations with 3 altitudes (<300, 300-600, >600 meter above sea level, as.). The results showed that there was not significant different of plant height, stem circle, crown circle, the number of primary branches, the number of flowers, the length of horizontal and vertical roots in response to different elevations. However, the height of primary stem and leaves number of cacao from <300 m asl was significantly greater than those from either 300-600 m asl or > 600 m asl. The largest crown portion of actual biomass was found in the branches, then followed by the leaves and stem. While the highest actual root biomass was found at 10-20 cm soil depth
Nonparametric Stability Analysis of Starch Content of Gamma Irradiated Cassava at Three Locations in West Java, Indonesia
Get PDFCassava is one of the largest starch producing tuber crops in Indonesia. Tapioca from cassava starch can be used as a raw materials for a variety of foods including biscuits, instant porridge, meatball, sausage, nuggets, and flour condiment. Breeding programs to improve cassava yield and starch content by using mutagen gamma rays irradiation was tested in four cassava generations (M1V4). Cassava is propagated by stem cuttings and the new characteristic obtained from gamma irradiation mutation is stable and can be passed from one generation to the next. Cassava mutants were obtained by evaluating the performance in different environmental conditions. The testing of yield adaptability and stability through a series of multi location is an important step before a new variety can be released. The aim of this research was to compare nonparametric stability and to evaluate the stability of the starch content of 16 genotypes (14 mutants and 2 varieties) in three locations in West Java Province i.e. Tapos (Depok), Cikarawang (Bogor), and Ciseeng (Bogor). Experiments were conducted in a completely randomized block design with three replications nested in each environment. Testing of yield stability in this study used four approaches with 10 parameters of nonparametric method. Starch content was estimated using gravimetric method. Based on frequency stability ranking of starch content from gravimetric method the G63142 genotype had the highest starch content estimation (29.99%), and the top five genotypes with high starch content were G63142, G61142, “Manggu”, G62151, and G63124. G61142 was categorized as genotype with static and dynamic stability, therefore this genotype is a very potential mutant to be released, whereas G63124 is categorized as a genotype with a static stability. Genotypes G63142, G62151, and “Manggu” had variable but relatively high starch content
Correlations of Nitrogen, Phosphorus, Potassium, Pigments and Total Flavonoids of Moringa oleifera Lam. Leaves in the Vegetative and Generative Phases
Get PDFMoringa oleifera is universally known as the miracle plant or the tree of life. Moringa leaf extract contains phenolic acids and flavonoids, especially flavonols (quercetin, kaempferol, isorhamnetin glycosides) and flavones (apigenin). This study aimed to elucidate any correlations between nutrient, pigment, and flavonoid concentrations for different leaf positions and different growth phases. The results showed that pigment and total flavonoid concentrations increased from the 1st leaf to the 5th leaf, while nutrient concentration decreased. Pigment concentration, total flavonoid, and nutrient concentration were higher in the vegetative phase than the generative phase. The concentration of nutrients in the generative plants displayed a significant positive correlation with chlorophyll concentration. The 1st to 5th leaf of the vegetative plants can be used as indicator leaves for tissue analysis of the moringa plant
Enrichment of Organic Manure with Plant Growth Promoting Rhizobacteria Improved the Root and Shoot Growth of Okra (Abelmoschus esculentus L. Moench.)
Get PDFA well-structured root system is essential to ensure optimal plant growth and yield. Two experiments were conducted to determine the effects of plant growth promoting rhizobacteria (PGPR) on the root system of okra plant. These experiments were arranged with a completely randomized design. The first experiment was conducted in the growth chamber with 8 different bacterial isolates consisting of Methylobacterium sp., Bacillus sp., Bacillus methylotrophicus, Flavobacterium tirrenicum, Providencia stuartii, Azotobacter vinelandii, Methylocystis parvus and PGPR consortium. The second experiment was conducted in the greenhouse and examined the effects of four poultry manure rates, i.e. 0, 6, 12 and 18 ton ha-1, or equivalent to 0, 75, 150 and 225% of recommended rates and how these are altered with the presence or absence of PGPR. The results of the experiments showed that, PGPR significantly improved root architecture; the number and length of lateral roots was increased by 242.86% and 777.79% respectively, as well as the dry weight of the roots and shoots of okra plant by 236.36% and 333.33%, respectively. Moreover applying 150% (12 t.ha-1) of the recommended rate of poultry manure enriched with PGPR was found to be most effective in terms of improving the growth and root attributes of okra plants
The Growth, Fruit Set and Fruit Cracking Incidents of Tomato Under Shade
Get PDFSix tomato genotypes were grown in the field under full sun at 50% reduced light intensity using shading net to evaluate growth, fruit set, and fruit cracking incident. The experiment was conducted during the rainy season in December 2016 to March 2017 in Cikarawang Experimental Station, Bogor, Indonesia. The genotypes tested were sensitive (“Tora” and F7005001-4-1-12-5), tolerant (F7003008-1-12-10-3 and F7003008-1-12-16-2), and shade-loving (SSH-3 and “Apel Belgia”). The results showed 50% shading delayed flowering and harvesting time in all genotypes. Genotype and shading treatments had an independent effect on fruit set. Shaded plants had lower flower abortion and resulted in a higher number of fruits per harvest, except in “Apel Belgia” and “Tora” genotypes. Fruit cracking incidents were low under shading implying the use of shading can increase tomato quality. However, it needs further investigation through using natural shading, e.g., intercropping system before this finding is applied in farmers’ field
Application of Cytokinin and Mycorrhiza to Increase Production and Quality of Pineapple Seedlings from Crown Leaf Bud Cuttings
Get PDFThe pineapple vegetative propagation method using crown leaf bud cuttings has potential to produce seedlings of greater uniformity and in larger quantities than other methods. This study, aimed to explore the potential eff ects of concentration and frequency of Benzylaminopurine (BAP) application for increasing the production and quality of pineapple seedlings from crown leaf bud cutting propagation. The experiment used a randomized complete block design (RCBD) with two factors. The fi rst factor was the concentration of BAP (0, 200, 400, 600, 800 ppm) and the second factor was the frequency of BAP application (1, 2, 3 times). Furthermore, this study investigated the eff ects of applying mycorrhizae to accelerate seedling growth and improve seedling quality during production from crown leaf bud cutting propagation. The experiment used a RCBD with one factor (dose of mycorrhiza: 0, 50, 100, 150, 200 spores). The results indicated that the eff ects of concentration and frequency of BAP application and their interaction increased seed production as determined by sprout cuttings at 5 weeks after planting by applying 600 ppm 2 times. Generally, with increasing concentration and frequency of BAP application, leaf chlorophyll content was reduced. However, the application mycorrhiza positively infl uenced the production of seedlings from pineapple crown bud cuttings and improved seedling quality as measured by root dry weight, chlorophyll content, root infection, and phosphatase enzyme activity
Characterization of a Drought-Inducible Dehydrin Promoter from Sugarcane (Saccharum officinarum L.) in Tobacco (Nicotiana tabacum L.)
Get PDFDehydrin (DHN) is known to play an important role in plant response and adaptation to abiotic stresses (drought, high salinity, cold, heat, etc.). Previous research reported the increased expression of DHN in sugarcane stems exposed to drought stress for 15 days which may be controlled by its corresponding stress inducible promoter. The DHN promoter was succesfully isolated from sugarcane variety PSJT 941 (Pr-1DHNSo) and was cloned to pBI121 expression vector fused to a β-glucuronidase (GUS) reporter gene. The aim of this research was the functional testing of the Pr-1DHNSo promoter through transformation into tobacco plant treated with in vitro drought stress. Genetic transformation of Pr-1DHNSo construct was conducted by Agrobacterium tumefaciens. The transformed tobacco was then subjected to drought stress treatment using 40% PEG 6000 for five sequential incubations (0, 12, 24, 48 and 72 hours). The GUS assay reveal that the transformed tobacco treated with drought stress showed a blue color denoting GUS activity in leaf, stem and root tissues and this expression increased along with the length of the drought treatment. The analysis of gusA gene using real time-qPCR normalized to the L25 reference gene also showed that the expression increased in line with the length of time of drought stress. The results presented in this study indicated that the Pr-1DHNSo promoter from sugarcane was expressed and induced by drought stress treatment in tobacco
Application of Rapid Multiplication Technique Using Mini Cutting and NPK Compound Fertilizer to Increase Production of Sweet Potato Cuttings (Ipomoea batatas L.)
Get PDFRapid Multiplication Technique (RMT) is a technique used to produce large scale cuttings of sweet potato in a short period by using mini cuttings and proper fertilizer management. The research was carried out from October 2019 to March 2020 and composed of two experiments The first experiment involved a randomized complete block design, with clones (“Ase Kapas” and “Ase Merah”) as the first factor, and tuber weight (150 ± 25 g, 250 ± 25 g, and 350 ± 25 g) as the second factor. The second experiment was arranged in a split-plot design involving different doses of NPK compound fertilizer (as main plot) and different source of cuttings (as subplots). The different doses included NPK 16:16:16 at 100 kg.ha-1 (0.3 g per polybag), 200 kg.ha-1 (0.6 g per polybag), 300 kg.ha-1 (0.9 g per polybag), and without fertilizer as control. The source of cuttings were from the tip, middle and bottom stem of the plants. Results showed that the “Ase Kapas” showed the highest number and the longest shoots, number of nodes, and length of vines. Additionally, tuber weight of ± 350 g produced the highest number of shoots. When it comes to NPK compound fertilizer treatment, a dose of 0.9 g per polybag increased cutting production in “Ase Kapas”, and also responded better to fertilizer treatment. On the other hand, dose of 0.3 g increased cutting production in “Ase Merah”. “Ase Kapas” produced more cuttings from the middle stem, whereas “Ase Merah” produced more cuttings from the . The application of RMT in “Ase Kapas” produced cuttings with ratio of 1:31, which is higher than those in “Ase Merah” with a ratio of 1:17.
 
Study of Intercropping System and In Situ Organic Matter Application on Coffee Agroforestry at Citarum Watershed, West Java, Indonesia
Get PDFAgroforestry-based coffee is one of the conservation efforts to restore the damages at the Citarum watershed. Planting annual crops between coffee rows could potentially contribute income for the growers during the immature non-productive coffee growth. The aims of the research is to study the effect of various types of agroforestry models in the upstream Citarum watershed on the growth of coffee, and growth and yields of the intercrops. The research was conducted in September 2018 to June 2019 at the upstream Citarum watershed of Tarumajaya Subdistrict Kertasari, Bandung Regency, West Java, Indonesia. The research was arranged with a randomized block design with two factors. The first factor was the cropping system, i.e. coffee monoculture, coffee and corn, coffee and sweet corn, coffee and red beans, and coffee and habanero chili. The second factor was organic matter application, i.e. with and without application of in situ organic matter. The results of the research showed that coffee intercropped with habanero with application of in situ organic matter had the best vegetative growth, indicated by taller plants, more leaves, increased leaf P and Mg levels, and the maximum income from the intercrop. Coffee intercropped with corn with application of in situ organic matter had a maximum corn production but had the lowest income. Therefore, the model of coffee intercropped with habanero chili with application of in situ organic matter was the best model from several intercropping systems tested
Organic Fertilizer Titonia Plus and Micro Nutrients Improved Rice (Oryza sativa L.) Production in Koto Panjang and Koto Tingga, Padang City, West Sumatera, Indonesia
Get PDFA series of on-farm fi eld experiments were conducted in two locations in Padang, Koto Panjang and Koto Tingga, West Sumatera, Indonesia in 2015. The purpose of the experiment was to establish the formula of organic fertilizer derived from Tithonia supplied with micro nutrients, Zn and Mn, on rice. The experiments were conducted using completely-randomized block design with six treatments and three replications. The treatments were P = Tithonia Organic Fertilizer Plus (TOFP) + 3.0 kg Mn.ha-1, Q = TOFP + 3.0 kg Mn.ha-1+ 3.0 kg Zn.ha-1, R = TOFP + 4.5 kg Mn.ha-1 + 6 kg Zn.ha-1, S= TOFP + 4.5 kg Mn.ha-1+ 9 kg Zn.ha-1, T = TOFP only, U = 100% chemical fertilizer only. Treatment with micro nutrients as addition to TOFP (TOFP+ 3.0 kg Mn.ha-1 and TOFP+ 3.0 kg Mn.ha-1+3.0 kg Zn.ha-1) increased rice grain yield by 80 g per clump