Journal of Tropical Crop Science
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Cloning and Characterization of P5CS1 and P5CS2 Genes from Saccharum officinarum L. under Drought Stress
No full textIncreasing world sugar demand might be fulfilled with land extensification which include the use of dry area. Development of drought tolerance and high productivity sugarcane variety could be achieved by plant genetic engineering. Under drought condition, proline will be accumulated and functioned as an osmoregulator in plant cells. ∆1-pyrroline-5-carboxylate synthase (P5CS) is one of the important enzymes in proline biosynthesis. This enzyme is encoded by P5CS gene family. We cloned two homologous P5CS genes from sugarcane, SoP5CS1 (Accession Number : KF178299) and SoP5CS2 (Accession Number : KF178300), which encode 729 and 716 amino acid polypeptides. The identity between these two genes was 74% based on nucleotide sequences. The SoP5CS1 gene had 98% identity with SbP5CS1 (Accession Number : GQ377719.1) and SoP5CS2 had 99% identity with SaP5CS (Accession Number : EF113257.1). In this experiment, sugarcane plantlets were exposed to medium containing PEG 6000 (40%) for 12, 24, 48, and 72 hours. Proline concentration was measured after treatment and genes expression were analyzed by real time-qPCR. The results showed that the proline concentration was increased 12 folds (9.8 umol.g-1) after 48-hours stress treatment. The highest expression of SoP5CS1 occured at 24-hours treatment with approximately 16 times from plant without PEG (control plant) and decreased gradually at 48 and 72 hours treatment. The highest expression of SoP5CS2 occured at 24-hours drought stress with approximately 3.6 folds compared to control. In drought treatment, the expression level SoP5CS1 was higher than SoP5CS2 and has increased significantly at 12-hours treatment. It is suggested that the SoP5CS1 gene contributes more significantly to the production of proline during drought stress than SoP5CS2. Hence, SoP5CS1 could potentialy be used as a marker to screen sugarcane variety for drought tolerance and for the development of transgenic plant tolerant to drought.Keywords: cloning, drought, expression, P5CS, sugarcan
Description and Ecology of Indonesian Species Tetrastigma glabratum (Blume) Planch, a host of Rafflesiaceae
No full textThis article presents an overview of the currentknowledge of Tetrastigma glabratum (Blume) Planch, ahost of Rafesiaceae, by providing information onbotany and ecology of T. glabratum, and conservationstatus by propagating the plants vegetatively andmonitoring its growth in-situ and ex-situ. The study wasconducted in the protected rainforests at Mount Prau,Candiroto, North Kedu, Central Java between 2009-2014. Shoot growth and expansion in stem diameterwere faster in-situ than ex-situ whereas the number ofleaves was similar in both locations. Ex-situconservation and cultivation is very important to protectthese rare, unique, and endangered species fromextinction
The Potential of Flowering Weeds as Refugia for Predatory Insects at Bantimurung-Bulusaraung National Park, South Sulawesi
Get PDFA study on identication of owering weeds as predatoryinsect alternative foods was conducted at Bantimurung-Bulusaraung National Park, South Sulawesi, Indonesiain February 2013. Among the plants that naturally growin the national park area Cassia siamea andClerodendron paniculatum, which were source of nectarfor insects, particularly butteries. Visits of predatoryinsect to owering weeds and species of both insectsand owering weeds were recorded. The preferredowering weeds visited by the predatory insects are C.striata (Papilionaceae), A.conyzoides (Asteraceae) andL. camara (Verbenaceae) followed by S. nodiflora(Asteraceae), S. rhombifolia (Malvaceae) and L.crustacea (Scrophulariaceae). A large number of adultsand pupae of Coccinella sp. was found in theBantimurung-Bulusaraung National Park.Keywords : nectar, pollen, alternative host, weeds,Indonesi
Reproductive success and compatibility among accessions of Jatropha curcas in Indonesia
Get PDFLarge scale exploitation of Jatropha curcas for biofuelproduction is restrained by low productivity henceeconomically not protable. One of the main constraintsis due to limited number of female owers in aninorescence which eventually develop into fruits thatbear seed. Investigation on oral biology andreproductive potential of the species had been carriedout, but seems to vary among sites. The research wasconducted to study reproductive success and thecompatibility among Indonesian Jatropha accessions.The research was set up in two experiments. The rstexperiment was conducted at the Jatropha seedorchard, Pakuwon, Sukabumi, West Java from April toJuly 2007 using four-year- old trees of accessions fromLampung, Banten, West Java, and Central Java. Thesecond experiment was conducted at LeuwikopoExperimental Station of Bogor Agricultural Universityfrom April to July 2008 using one-year-old trees ofaccessions from Lampung, Bengkulu, Palembang, andKediri. Flowers were obtained from controlled self andcross-pollinated as well as left for open-pollination. Theresult showed that reproductive success varied amongaccessions; the greatest success was 0.73 obtainedfrom aWest Java accession and the least was 0.53 fromBanten accession. The Jatropha accessions werecategorized as partially self-incompatible as indicatedby index of self-incompatibility (ISI) that ranged 0.93-0.99. Banten accession produced highly viable andvigorous seeds regardless of pollination methods;accessions of Central Java produced higher viabilityseeds when cross-pollinated within accession, whereasthose from West Java and Lampung produced higherviability seeds when self-pollinated.Keywords: accessions, Jatropha curcas, selfpollination,self-incompatibility, seedviabilit
The Usefulness of Molecular Markers Approach for Developing Heterotic Groups in Maize
Get PDFThe phenomenon of heterosis provides a greatopportunity for plant breeders to gain greaterperformance and yield in the hybrids compared to theirinbred line parents. In most cross-pollinated crops likemaize, heterosis plays an important role as theperformance of the hybrids is of a great value. Heterosisgain much interest until recently and current studies stillattempt to elucidateone of these is utilizing molecularmarkers to estimate genetic distances among inbredlines followed by developing putative groups. In a welldened heterotic group, between-groups mating areexpected to produce better hybrids than within-groupsmating. Some studies of marker-aided heterotic groupdevelopment resulted in functional heterotic groups;while some other reported that the groups failed toprovide evidence of heterosis in the hybrids.Considering the prediction of hybrids' performance willdepend on genetic background of inbred lines, andmarkers technology are continuously improved, there isstill a good opportunity to obtain useful heterotic groupsfor a particular breeding population.Keywords: maize breeding, genetic distance, heterosis,molecular markers