Biovelentia - Biological Research Journal
Not a member yet
64 research outputs found
Sort by
ANTIBACTERIAL ACTIVITY STUDY OF ACTIVE FRACTION FROM CHICK WEED PLANTS (Ageratum Conyzoides L.) AGAINST Bacillus Subtilis AND Vibrio Cholerae
No full textThe purpose of this research to determine the fractions of Chick Weed which has strong antibacterial activity against bacteria test categories of Bacillus subtilis and Vibriocholerae.determine the value of the minimum in hibitory concentration(MIC) of the active fraction antibacterial Chick Weed.This research was carried out in August up to November 2016. The method used in this study were extracted by maceration, fractionation by liquid-liquid fractionation, separation by column chromatography fractions, antibacterial activity test by theKirby-Bauermethod, while the determination of minimum in hibitory concentration by dilution broth,with test bacteria Bacillus subtilis and Vibriocholerae.The data presented in tabular form based on the average value and percent.The results of this study showed that the methanol extract Chick Weed active against test bacteria Bacillus subtilis and Vibrio cholerae. Fractionation which has strong category to standard antibiotics are methanol fraction by fraction column S4.The concentration MIC1000;500;250;125;62.5;31.2515.62; 7.81 ppm. The minimum in hibitory concentration column fractions S4 to test bacteria Vibrio cholerae of 62.5 ppm gives half the antibacterial activity of the antibacterial activity of standard antibiotics streptomycin and penicillin,tetracycline while giving a quarter activity. It can be concluded that the active fraction of methanol extractisa methanol fraction by fraction column S4 to test bacteria Vibrio cholerae.Keywords: Chick Weed, Minimum Inhibitory Concentration (MIC), active compound, Bacillus subtilis, Vibriocholera
STUDY ON MACROZOOBENTHOS COMMUNITY IN THE WATERS OF THE RIVER AIR PERIKAN MUNICIPALITY OF PAGAR ALAM SOUTH SUMATRA
No full textThe research entitled “ Study on Macrozoobenthos Community in the Waters of the River Air Perikan Pagar Alam Municipality”, was conducted from November 2007 until March 2008. The aims of the research: to know about composition, density, diversity index, dominancy index, and similarity index, which based on the different microhabitat types. Sampling was carried out on November 2007. Five sampling stations were determined by survey method and the Purposive Sampling method was used at each sampling point to find stony, gravel, sandy and leaf pack area as a different microhabitat substrate. Twenty one orders (Ephemeroptera, Trichoptera, Diptera, Plecoptera, Collembola, Hemiptera, Odonata, Coleoptera, Lepidoptera, Megaloptera, Hymenoptera, Orthoptera, Decapoda, Plesiopora, Tricladida, Amphipoda, Isopoda, Mesogastropoda, Ctenobranchiata, Eulamellibranchiata, and Rhynchobdellida) which are consisted of 70 families and 151 genera were identified from four types of substrates on each sampling station. According to this research, station 4 has the highest in composition (80 genera), and the lowest one at station 1(33 genera). The highest abundance was 12589 ind./m2 found in the station 5, and the lowest one was in the station 1 approximately 880 ind./m2. For the diversity index value, station 3 was the highest (3.1) and the lowest one was station 5 (1.9). The Dominancy index was found in station 5 at the sandy substrates was approximately 0,8 index, which dominated by Tubifex sp. There were differences species composition on each station. Keywords : Macrozoobenthos, community, the river Air Perikan
THE ANTIBACTERIAL ACTIVITY OF WATER APPLE LEAVES ACTIVE COMPOUND (Syzygium zeylanicum) AGAINST Escherichia coli AND Staphylococcus aureus
No full textEscherichia coli is one of the bacteria that cause infections in the human digestive tract such as diarrhea, while Staphylococcus aureus is one of the bacteria that cause infections in the skin injury such as boils and pimples. This study used Syzygium zeylanicum leaves because it has potential as a antibacterial because it contains active compounds. This study aimed was determine the antibacterial activity of the fraction and the active compound in Syzygium zeylanicum leaves against Escherichia coli and Staphylococcus aureus. Research conducted on November 2015 to January 2016. The method used in this research were extraction by maceration, fractionation by liquid fractionation, antibacterial activity test, and determination of minimum inhibitory concentration with the diffusion method and isolation of active compounds by column chromatography method. The bacteria used in this test are Escherichia coli and Staphylococcus aureus. Data are presented in tabular form based on the average value of the inhibition diameter and deviation standard. The results of this research showed the water methanol active fraction against the bacteria that used in this test. The methanol water fraction had obtained one antibacterial compound in bottle 1,3,5 which shows the value of tannin Rf 0,416. The minimum inhibitory concentration of water methanol of water apple leaves is 1000 µg/mL for Escherichia coli and 500 µg/mL for Staphylococcus aureus. The minimum inhibitory concentration of the active compound to Escherichia coli and Staphylococcus aureus in 500 µg/mL. The fraction and the active compound of water apple leaves have an antibacterial activity with Escherichia coli and Staphylococcus aureus and the active compound is tannin
CONCENTRATIONS TEST OF METHANE GAS FROM SWAMP FORAGE SILAGE WITH IN VITRO METHOD
No full textAims of this study to concentration test of methane gas from swamp forage silage with in vitro methods.This study used Completely Randomized Design with 3 treatments and each treatment consisted of 5 replications. The treatments used are as follows: P1 (100% kumpai tembaga grass (Hymenachne acutigluma)), P2 (50% kumpai tembaga grass (Hymenachne acutigluma) + 50% kemon air (Neptunia oleracea lour)), P3 (100% kemon air ( Neptunia oleracea lour)). The parameters observed N-Ammonia, total gas production, methane gas concentrations in vitro and VFA partially. The results of variance showed that treatment significantly different (P <0.05) with methane concentration in vitro with highest gas concentration treatment of silage made from 50% kumpai tembaga grass (Hymenachne acutigluma) and 50% kemon air (Neptunia oleracea lour).Keywords: Concentration, Methane, Silage Forage Swamp, In Vitro
HABITAT OPTIMALIZATION OF CAPTIVE DEER PT. PUSRI PALEMBANG
No full textDeer is one of the species that going through a reduction of population because its being used as the target of illegal hunting. To prevent the reduction in population, the efforts to save them with conservation method both in-situ or ex-situ has been done. Captive deer in PT. Pusri Palembang is the only captive deer which located in urban areas of Palembang and carrying out an ex-situ conservation effort. This research aimed to analyze the habitat condition such as daily feed, water needed, and shelter, to know about the level of palatability and also to analyze the habitat condition as a basic of captive deer management towards an optimalization. The research has been done in January 2015. The interview method, literature study and field observation are used to get the data about population and habitat condition in captive deer PT. Pusri Palembang. It can be concluded that the habitat condition in the captive deer PT. Pusri Palembang still quite good, and 1,5 hectare areas still be able to maintain the total of 44 deers (22 sambar deers and 22 chital deers) in 2015.Key words: sambar deer, chital deer, captivity, ex-situ conservation, PT Pusri Palembang.
VEGETATION ANALYSIS ON RECLAMATION AREA OF COAL MINE OF PT. BUKIT ASAM TANJUNG ENIM, SOUTH SUMATERA
No full textThe Research of Vegetation Analysis on Reclamation Area of Coal Mine of PT. Bukit Asam Tanjung Enim, South Sumatera was aimed to determine the types of vegetation that composing reclamation area was found in PT. Bukit Asam in terms of structure and composition. The research has been done in November until Desember 2014, in four reclamation area at PT. Bukit Asam, namely the area of reclaimed the Muara Tiga Besar Selatan, Mahayung, Klawas Timur, and the Muara Tiga Selatan having different strata age. the vegetation data includes rate of growth seedlings, saplings, poles, and tree obtained through transek method in the form plot lane. In each transect made a plot in size 20x20 m for tree level, 10 x 10 for poles level, 5x5 for saplings level, and 2x2 m for seedling and cover plants level. The results of this research, in the Muara Tiga Besar Selatan area was obtained the vegetation seedlings-cover plants and saplings level, each of 17 and 7 species. In the area Mahayung are only found level vegetation of seedlings-cover plants as many of 13 species. For the area Klawas Timur and Muara Tiga Besar Selatan was obtained growth level of seedlings-cover plants, saplings, poles, and tree each of 20, 4. 3, dan 2 species for Klawas Timur area, while on Muara Tiga Selatan area each obtained 19, 8, 7, and 3 species.The diversity index of species, overall in all area of reclamation and rate of growth included in the low category with value 0 ≤ H’ ≤ 2.Key words : PT. Bukit Asam, Vegetation Analysis, Reclamation, Transect, Diversity Inde
THE SECRETORY STRUCTURE OF ESSENTIAL OILS IN SOME SPECIES OF LAMIACEAE FOR TRADITIONAL COSMETICS OF BESEMAH TRIBES LAHAT’S DISTRICT
No full textBesemah tribe uses plants in addition to the treatment is also used for beauty treatments traditionally. Based on Agustina's (2015) study, 109 species of plants were used for traditional medicine by the Tribe of Besemah. Some species of Lamiaceae for traditional medicine such as patchouli (Pogostemon cablin (Blanco) Benth.), wild mint (Mentha arvensis L.), hoary basil (Ocimum americanum L.), mexican mint (Plectranthus amboinicus (Lour.) Spreng.), and sweet basil (Ocimum basilicum L.) contains essential oils that can be used for traditional cosmetic ingredients. Essential oils are stored in the secretory structure of plants in leaf and stem organs. This study aims to determine the type and location of the secretory structure of essential oils in some species of lamiaceae used for traditional cosmetics of Besemah Tribes Lahat’s District. The research was conducted in November 2016 until February 2017. The results showed that patchouli plants was found in the presence of glandular trichomes, oil cells, and idioblast cells. The wild mint plants have glandular trichomes. The hoary basil plants have glandular trichomes and oil cells. The mexican mint plants have glandular trichomes and idioblast cells. The sweet basil plants have glandulartrichomes.Keywords: essential oils, lamiaceae, traditional cosmetics, Besemah tribes, glandular trichomes, oil cells, idioblast cell
THE INFLUENCE OF DOLOMITE GIVING ON INDIGENOUS BACTERIA NITROGEN INHIBITOR IN TIDAL SOIL HAS BEEN MANAGE FOR OVER 30 YEARS
No full textThe study was conducted to determine the effect of land typology and lime on the population of indigenous nitrogen-fixing bacteria and soil pH, and to analyze the effect of soil typology interaction and lime on the population of indigenous nitrogen-fixing bacteria and soil pH. Sampling of soil in tidal land of Mulya Sari and Banyu Urip Village of Banyuasin Regency, South Sumatera. Then the sample was studied at the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Inderalaya. During January-May 2017. Based on the results of the study known the soil typology of the total population of nitrogen-fixing bacteria, the highest population of nitrogen inhibitor bacteria in the second week of soil typology D was 6.0x107 and the highest pH of 5.18 in typology B on week Third, the lime treatment that affected the population of indigenous nitrogen-fixing bacteria, obtained the population of bacteria as much as 9.3x105 in the second week and the highest soil pH 4.93 in the second week, while the results of the interaction between soil typology and lime treatment only affect the population of bacterial inhibitor Indigenous nitrogen, with bacterial population of 3.5x107 in soil typology D in the second week, but this interaction did not significantly affect soil pH, with the highest pH measurement of 5.33 on the soil typology D.Keywords: nitrogen-fixing bacteria, tidal land, lime treatmen
HORMESIS PHENOMENA OF SOME PARAMETER ASPECT IN USAGE OF WATER LETTUCE (Pistia stratiotes L.) FOR PHYTOREMEDIATION PROCESS OF PETROLEUM LIQUID WASTE
No full textThe research about potential test of water lettuce (Pistia stratiotes) in order of phytoremediation of petroleum liquid waste had been done during June untill December 2012 at Microbiology Laboratory, Department of Biology, Faculty of Science, The University of Sriwijaya, Indonesia. Completely Randomized Design was used in the experiment while the concentration of liquid waste as treatment were: 0, 15, 30, 45, 60 and 75 %. Each treatment were replicated 4 times. Three parameter was measured; Total Petroleum Hydrocarbons (TPH) reduction percentage, efficiency of phytoremediation and number of tillers. Regression analisys were used for the data and two of parameters show the hormesis phenomena, were; efficency of phytoremediation and number of tillers. The highest peak effect of treatment for efficiency of phytoremediation and number of tillers was on 45 %. TPH reduction percentage was the only one parameter that shows linier regression Keywords hormesis, Pistia stratiotes, TPH, phytoremediation, tille
FOOD HABITS AND LENGHT-WEIGHT RELATIONSHIP OF LUKAS FISH (Labiobarbus leptocheilus Valenciennes) IN GAJAH MUNGKUR RESERVOIR CENTRAL JAVA
No full textThe Research about the food habits and length-weight relationship of Labiobarbus leptocheilus Valenciennes was conducted from May to September 2010. The aims of this research were to find out the food habits and to analyze the growth pattern of Labiobarbus leptocheilus Valenciennes. The sampling of fishes was conducted on May 2010 in the Gajah Mungkur Reservoir, Central Java. The sampling of fishes was conducted by using survey on the landing place of fishes (TPI Mina Tirta). The methods of analysis of food habits by using the Index of preponderance. The results of this research obtained were 19 samples of Labiobarbus leptocheilus Valenciennes, the length between 15-20 cm, with the weight between 40-90 grams. Labiobarbus leptocheilus Valenciennes had the negative allometric growth pattern (b <3). The laboratory analysis of food habits showed that the food habits of Labiobarbus leptocheilus Valenciennes were detritus with Index of Preponderance (IP) 82.95% and plankton with IP 17.05%.Keywords: food, habits, length-weight, relationship, Labiobarbus leptocheilu