SUNY College of Environmental Science and Forestry
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Fred O\u27Neil receiving Container Corps of American check
No full textFred O\u27Neill receiving a Container Corps of America check from two Caucasian males
3396 x 4561https://digitalcommons.esf.edu/paperimages/1024/thumbnail.jp
Walters Hall and circle parking lot from north view
No full textA northern view of Walters Hall and a circle parking lot next to it
4767 x 4853https://digitalcommons.esf.edu/paperimages/1041/thumbnail.jp
Students examine paper in paper science program
No full textTwo white, male students in the paper science program standing with a professor examining paper
4950 x 4771https://digitalcommons.esf.edu/paperimages/1062/thumbnail.jp
Paper Science Engineering students
No full textFive male Paper Science Engineering students working in a lab
5822 x 4618https://digitalcommons.esf.edu/paperimages/1078/thumbnail.jp
Unknown person at cylinder paper former in original part of paper lab building- sub basement
No full textA white male working with a cylinder paper former in the original part of the paper lab building in the sub basement
5779 x 4586https://digitalcommons.esf.edu/paperimages/1082/thumbnail.jp
Refiner on far left, screw press on right- south end of paper machine room
No full textAn unknown male working in the south end of the paper machine room with the refiner (far left), screw press (on right)
6004 x 4785https://digitalcommons.esf.edu/paperimages/1149/thumbnail.jp
Student working with machine
No full textAn unknown male sitting down using a machine
6005 x 4797https://digitalcommons.esf.edu/paperimages/1157/thumbnail.jp
Biochemical Characterization of the Enhancer-Binding Protein DdaR of Pseudomonas aeruginosa
No full textPseudomonas aeruginosa is a bacterium that forms biofilms in the lungs of individuals with cystic fibrosis. Biofilm formation is regulated by nitric oxide synthase, which is known to produce nitric oxide from the substrate L-arginine. Pathogenic bacteria are released from biofilms in the presence of nitric oxide. A product of methylarginine metabolism in P. aeruginosa is NG,NG-dimethyl-L-arginine (ADMA), which inhibits nitric oxide synthase and prevents the formation of nitric oxide. The PA1195 (ddaH) gene of P. aeruginosa encodes a dimethylarginine dimethylaminohydrolase (DdaH) enzyme for methylarginine degradation, such as ADMA to L-citrulline. The protein DdaR may act as an enhancer-binding protein (EBP) and regulate the activation of σ54-dependent transcription for this gene. In this study we heterologously expressed the DdaR protein as an N-terminal Maltose Binding Protein (MBP) fusion-protein in Escherichia coli BL21 cells, and subsequently purified the MBP-DdaRdbd fusion-protein using amylose affinity chromatography. Polymerase chain reaction (PCR) was used to replicate the DNA probes ZS458 and ZS406. The ZS458 probe contains the ddaH promoter sequence and is the expected target promoter of DdaR. The ZS406 probe contains the gcvH2 promoter. DdaR is not expected to bind to this probe and hence it will serve as a negative control probe. The ZS406 and ZS458 probes were purified at a concentration of 9.1 ng/μL and 8.2 ng/μL, respectively. Future research will focus on utilizing the purified DdaR protein and ZS458 and ZS406 probes in electrophoretic mobility shift assays (EMSAs) to determine if DdaR binds to the promoter region of the ddaH gene in P. aeruginosa
Development and Validation of Rapid eDNA Detection Methods for Bog Turtle (Glyptemys muhlenbergii)
Get PDFBog turtles (Glyptemys muhlenbergii) are listed endangered species in the United States. Multi-state efforts are underway to better characterize extant populations of the species and prioritize restoration efforts. Traditional sampling methods for bog turtles can be ineffective due to their wetland habitat, small size, and burrowing nature. New molecular methods, such as qPCR, provide the ability to overcome this challenge by effectively quantifying minute amounts of turtle DNA left behind in its environment (eDNA). Developing such methods for bog turtles has proved difficult partly because of the high sequence similarity between bog turtles and closely-related, cohabitating species, such as wood turtles (Glyptemys insculpta). Additionally, substrates containing bog turtle eDNA are often rich in organics or other substances that frequently inhibit both DNA extraction and qPCR amplification. Our first goal was to develop a qPCR assay that could correctly identify blood collected from seven species of turtle over a wide geographic range. The eDNA detection method was primarily validated using contrived positive samples from the environment. Furthermore, methods employing a genetically modified strain of C. elegans as a full-process internal control which was used in method optimization and to determine DNA recovery from field sample
THE WHITEWASHING OF WILDERNESS: HOW HISTORY AND SYMBOLIC ANNIHILATION INFLUENCE BLACK AMERICANS\u27 PARTICIPATION AT NATIONAL PARKS
Get PDFVarious publications argue that National Parks are not ethnically diverse amongst visitors and personnel, particularly amongst Black Americans. Although the most cited causes for lack of visitation to areas include cost, lack of access, lack of knowledge, and racial bias, more research is needed on how National Park promotional materials impact Black American visitors. This research offers an assessment of Black environmental attitudes regarding outdoor recreation with interviews being the primary method of data collection. Additionally, to investigate how park guides influence Black Americans’ attitudes and intentions towards visiting National Parks, a content analysis was performed. This research aims to fill the gap in information on how the whitewashing of wilderness has impacted Black Americans’ intentions to participate. Preliminary findings demonstrate that there is a symbolic annihilation of Black people in National Parks and that impacts Black Americans intentions; therefore, lessening their intentions towards visiting National Parks