Dataverse UNIMI
Not a member yet
601 research outputs found
Sort by
ReData: 1912 - Daily meteorological bulletins edited by the Italian Royal Central Meteorological Office
No full textThis dataset contains the daily meteorological bulletins edited by the Italian Royal Central Meteorological Office relative to the year 191
Confidential deliverables generated in the frame of NoPEST project
No full textThe NoPest project generated several confidential deliverables related to key research areas:
Peptide Aptamer and Peptidomimetic Libraries
Creation of linear and cyclic peptide aptamer libraries for screening.
Compilation of interacting peptide aptamers from D1.1 and D1.4 targets.
Sets of peptide aptamers derived from D2.2 and D2.3.
Libraries of peptidomimetics obtained from D5.2 and D5.3.
Collection of modified peptides from constrained peptide design.
Identification of a set of non-peptidic hits.
Application and Efficacy Testing
Field application of non-invasive sensing technologies.
In vitro estimation of the antimicrobial efficacy of aptamers from D3.1 and D3.2.
Evaluation of peptidomimetics (with or without CPP) and non-peptide small molecules in vitro, including antimicrobial efficacy and phytotoxicity assessments.
Study of the effects of peptidomimetics, CPP, and non-peptide small molecules on non-target organisms.
Toxicological and eco-toxicological assessment of antimicrobial molecules identified during NoPest.
In Planta Studies
Evaluation of pathogen inhibition in planta by formulated peptidomimetics and non-peptide small molecules in Italy, the Netherlands, and Germany.
Study of the mobility of peptidomimetics and non-peptide molecules within plant tissues.
Dissemination and Exploitation
List of events dedicated to the dissemination and exploitation of project findings
Replication Data for: Astrocytic ATM deficiency triggers complement C3a-mediated synaptic dysfunction and depressive-like behaviors
No full textThis dataset includes LC-MS/MS data of differentially expressed proteins in the astrocyte proteome and secretome generated in the study entitled “Astrocytic ATM deficiency triggers complement C3a–mediated synaptic dysfunction and depressive-like behaviors” by Sabrina Briguglio, Anna Selimi, Clara Cambria, Ramona Stringhi, Manuela Moriggi, Mario Federici, Maria Vittoria Zavaglia, Elena Borroni, Francesco Rusconi, Elena Battaglioli, Elena Marcello, Laura Musazzi, Daniele Capitanio, and Flavia Antonucci.
Astrocytes were gently scraped, collected in PBS and pelletted. Astrocytes were then suspended in lysis buffer (2% SDS, 100 mM Tris-HCl pH 7.6, 0.1 M DTT, and 1% phenylmethanesulfonylfluoride) and sonicated on ice until completely dissolved. After incubation at 95 °C for 3 min, lysates were clarified by centrifugation at 12 000 g for 20 min at 4 °C. Protein quantitation with 2-D Quant-kit protein assay (Cytiva) was then performed. Culture media (15 ml) were centrifuged in 30 KDa MWCO filters (ThermoFisher scientific) to a final volume of 50 µl. Lysis buffer (50 µl) was then added, and each medium was processed as described for cells. Protein extracts (50 µg for each sample) were processed following the filter-aided sample preparation (FASP) protocol. Peptide samples were concentrated and then separated on a Dionex UltiMate 3000 HPLC System with an Easy Spray PepMap RSLC C18 column (250 mm, internal diameter of 75 µm) (ThermoFisher Scientific), and finally electrosprayed into an Orbitrap Fusion Tribrid (ThermoFisher Scientific) mass spectrometer. Three technical replicates for each sample were acquired.
Mass spectra were analysed using MaxQuant software (Max Planck Institute of Biochemistry, Munich, Germany, version 1.6.17.0). The maximum allowed mass deviation was set to 6 ppm for monoisotopic precursor ions and 0.5 Da for MS/MS peaks. Enzyme specificity was set to trypsin/P, and a maximum of two missed cleavages was allowed. Carbamidomethylation was set as a fixed modification, while N-terminal acetylation and methionine oxidation were set as variable modifications. Spectra were searched by the Andromeda search engine against the Mus musculus Uniprot UP000000589 sequence database (54707 proteins, March 2024). Protein identification required at least one unique or razor peptide per protein group. Quantification in MaxQuant was performed using the built-in extracted ion chromatogram (XIC)-based label-free quantification (LFQ) algorithm using fast LFQ. The required FDR was set to 1% at the peptide, 1% at the protein, and 1% at the site-modification levels, and the minimum required peptide length was set to 7 amino acids. Statistical analyses were performed using Perseus software (v.1.6.15.0, Max Planck Institute of Biochemistry, Martinsried, Germany). For each experimental group, proteins identified in at least 80% of samples were considered. False positives were reduced utilizing the Benjamini–Hochberg false discovery rate test
Agricultural season 2020
No full textThis dataset contains data collected during the 2020 agricultural season in the Lower Mondego Valley case study (Portugal). The experiment was conducted in pilot fields located on two experimental farms: Quinta do Canal (Alqueidão) and Bico da Barca (Alqueidão). At each farm, two experimental irrigation strategies were tested: (1) wet seeding with continuous flooding (WFL) and (2) wet seeding with alternate wetting and drying (AWD). The dataset also includes the experimental field layouts and details on the cultivated rice varieties
ReData: 1936 - Daily meteorological bulletins edited by the Italian Royal Central Meteorological Office
No full textThis dataset contains the daily meteorological bulletins edited by the Italian Royal Central Meteorological Office relative to the year 193
Agricultural season 2019
No full textThis dataset includes data gathered during the 2019 agricultural season in the Lis Valley case study (Portugal). The experiment was carried out in a pilot field located at the Nuno Guilherme pilot farm under a wet seeding and continuous flooding irrigation regime. The dataset also contains the experimental field layouts and information on the cultivated rice variety
Replication Data for "Contrasting elevation-dependent effect of snow cover on nest-box use in a cold-adapted alpine specialist, the white-winged snowfinch Montifringilla nivalis"
No full textReplication data for the paper "Contrasting elevation-dependent effect of snow cover on nest-box use in a cold-adapted alpine specialist, the white-winged snowfinch Montifringilla nivalis". Wildlife Biology. doi: 10.1002/wlb3.0155
Agricultural season 2019
No full textThis dataset contains data collected during the 2019 agricultural season as part of the Baix Ter case study (CS2), where three irrigation strategies were tested: (i) wet seeding with continuous flooding (WFL); (ii) dry seeding with delayed flooding starting from the three-leaf stage (DFL); and (iii) dry seeding with subsurface drip irrigation (SDI). WFL and DFL were applied at the Mas Pla pilot farm, located in the Pals area within a traditional rice-growing region. SDI was implemented at the Camp del Cobert pilot farm, also in Pals, but outside the conventional rice cultivation area. The dataset also includes the experimental layouts of the FL and SDI pilot fields, as well as the rice varieties cultivated in those fields
Replication Data for: "Small-Molecules as Chemiluminescent Probes to Detect Lipase Activity"
No full textExcel raw data from kinetic analyses as described in the Readme file.
Luminometric analyses were carried out in 96-well white clear-bottom plates (Corning) using a Varioskan LUX Multimode Microplate luminometer Reader (Thermo Fisher Scientific, USA).
Data from kinetic experiments were extracted in MS excel files, all analyses were performed by MS excel.
Tabs report Luminescence intensity, expressed as Relative Luminescence Units, released by compounds 2-4, released by enzymatic hydrolysis (esterase or different lipases), and chemically rearranged.
Experiments were performed in Tris-HCl buffer pH 7.4, 1mM EDTA in the presence (0.2 U/well) or in the absence (no enzyme) of three different lipases, CCL, PFL, and MML, and PLE as control. Varioskan LUX Multimode Microplate Reader (Thermo Fisher Scientific, USA) was used to measure light emission every 2 min for 1.5 h. Assays were performed in total white flat bottom microplates in a total volume/well of 200 L Tris-HCl, 1mM EDTA (10% DMSO), with 10 Mfinal concentration of the assessed compounds (2, 3, and 4).
CCL= Lipase from Candida cylindracea
MML=Mucor Miei Lipase
PFL= Pseudomonas fluorescens Lipas
Replication data for 'Looking for BVER into the GRASS'
No full textData and code used for the development of an archaeological inductive predictive model in the southern lake Maggiore (Verbano) area, between Lombardy and Piedmont, Italy. BVER is for "Basso VERbano" (Lower Verbano).
The inductive predictive model was originally created as a part of a full scale archaeological research in the area, supported by the Cattedra di Archeologia Cristiana, Tardoantica e Medievale (Chair of Christian, Late Antiquity and Medieval Archaeology) of the University of Milan, department of Cultural and Environmental Heritage.
The current work investigates the settlement dynamics of the lower Verbano area between the Iron Age and the Middle Ages. The known archaeological sites, obtained from the public database GNA (Geoportale Nazionale per l'Archeologia), together with a series of physical characteristics of the area, were used as predictors for the calculation of an inductive predictive model. The results show the shortcomings of this methodology in the field of predictive archaeology without an ethnoarchaeological premise.
The model is primarily intended to test the reliability of inductive predictive modelling in understanding ancient settlement patterns. The results will then be further discussed and developed