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Replication Data for: lactate of "Replicative Senescence in Mesenchymal Stem Cells: An In Vitro Study on Mitochondrial Dynamics and Metabolic Alterations"
No full textThe luminescence-based Lactate-Glo™ Assay (J5021, Promega) was used to quantify intracellular lactate. Specifically, lactate dehydrogenase uses lactate and NAD+ to produce pyruvate and NADH. In the presence of NADH, a pro-luciferin reductase substrate is converted by reductase into luciferin, which is subsequently used in a luciferase reaction to generate light. Therefore, the light emission is proportional to the amount of lactate in each sample. bMSCs were seeded in a 6-well plate (Costar, Sigma-Aldrich) (1.5 × 105 cells/well) and, after 3 days, were detached from the plate and counted for normalization. Cells were then incubated for 5 min at room temperature with Inactivation Solution (0.6 N HCl in H2O) which rapidly stops metabolism, lyses the cells, inhibits activity of endogenous proteins and destroys reduced NAD(P)H dinucleotides. After inactivation, Neutralization Solution (1 M Trizma®) was added to each sample. The samples were aliquoted in duplicate into a 96-well white plate (Costar, Sigma-Aldrich). At the same time, Lactate Detection Reagent was prepared according to the manufacturer’s instructions and added to each well. The plate was incubated for 1 h at room temperature in the dark. Luminescence was measured using a Varioskan LUX Multimode Microplate Reader (Thermo Fisher Scientific). The results were normalized to the cell number
Replication Data for: Energy-Aware Assignment Patterns in Multi-access Edge Computing
No full textReplication Data for: Energy-Aware Assignment Patterns in Multi-access Edge Computing. Please refer to the README.md file for full details on the dataset
Work Data for Story Board/Prima del Maestro-Count down n.1
No full textStory board formato da disegni per animazione in computer grafica denominato per "Prima del Maestro", Count down classical music", RaiSat,1991. Foto documentative di Nicola Gronchi
Replication Data for: "Supporting Young Adults’ Life Choices: A Longitudinal Perspective on the Role of Welfare Effort Across Crises"
No full textYoung adults’ life choices and patterns of their permanence in the parental house
are extraordinarily heterogeneous across European countries. What is the role of
the welfare state in accounting for such diversity and how has it changed over time?
This article addresses these questions by relying on regression models covering 31
European countries over the period 2005–2022. Deploying age-sensitive welfare
effort measures, the study provides fresh empirical evidence about the ‘welfare
matters’ hypothesis, confirming that even when controlling for a large set of
economic, social, political and cultural variables, youth welfare effort remains a
highly significant factor. Furthermore, our findings show that its role increased in the
wake of crisis periods such as the Great Recession and the COVID-19 pandemic,
producing additional divergence among countries. From a life-course perspective,
these results suggest that targeted social spending may be an effective investment
for promoting young people’s paths towards autonomy
Data for: DAW Usage Survey (2023)
No full textThis dataset, available in JSON format, contains the responses to a worldwide survey conducted from December 2023 to January 2024 on the usage of Digital Audio Workstations (DAWs). The goal was to study the habits of experts in audio production. The survey involved 615 people, including musicians, audio technicians, and scholars in the field of sound and music computing. The JSON file contains survey responses regarding the use of Digital Audio Workstations (DAWs). Each entry represents an individual respondent and consists of two main sections: PersonalData (user profiling with no sensitive data) and UseCases (a list of cases where the respondent has used DAWs), as further explained in the attached readme file.Data have been collected through a web form available at collection or generation (include links or references to publications or other documentation containing experimental design or protocols used). Data are presented in a raw, unprocessed format.Since JSON is a plain-text format, no proprietary software or instrument-specific information is needed to understand or interpret the data. Any digital text editor can open the dataset. Nevertheless, software capable of JSON formatting is recommended.
The dataset has been presented and analysed in a conference paper presented at the 22nd Sound and Music Computing Conference (SMC 2025).
Doi: 10.5281/zenodo.15843017</p
Replication Data for: Identification of neuronatin as a SERCA2b regulin-like protein and assessment of its aggregation propensity via coarse grained simulations
No full textThis dataset supports the study "Identification of neuronatin as a SERCA2b regulin-like protein and assessment of its aggregation propensity via coarse grained simulations". It provides all the structural and dynamic data necessary to reproduce and extend the computational results described in the publication.
The dataset includes:
Molecular docking poses of neuronatin (NNAT) on the SERCA2b calcium pump;
Molecular dynamics simulations of SERCA2b alone and in complex with NNAT, both in solution and membrane environments;
Aggregation simulations of NNAT variants using all-atom and coarse-grained models.
All simulations were performed using Desmond and converted to open formats compatible with free software. The files are intended for researchers interested in protein–protein interactions, membrane protein dynamics, and the biophysical behavior of intrinsically disordered proteins.
The files contained in the dataset are in the following formats`.pdb`: Structure file (used as topology); and `.xtc`: Trajectory file (compressed, portable format). For more details on how to open it, please refer to the attached readme file
Replication Data for: triglycerides of "Replicative Senescence in Mesenchymal Stem Cells: An In Vitro Study on Mitochondrial Dynamics and Metabolic Alterations"
No full textThe Triglyceride Quantification Kit (MAK266-1KT, Sigma-Aldrich) was used to quantify TGs in bMSCs. The cells were seeded in a 6-well plate (Costar, Sigma-Aldrich) (1.5 × 105 cells/well). After 3 days, the cells were lysed in 1 mL of H2O + 5% NP-40 and centrifuged for 2 min at 13,000 rpm. Cells (6 × 103) were plated in triplicate in a 96-well black plate (Costar, Sigma-Aldrich). Lipase was then added to each well for 20 min at room temperature. The glycerol generated from the hydrolysis of TGs was then oxidized by adding the Master Reaction Mix provided in the kit, resulting in the generation of a fluorescent product (λex/λem = 535/587 nm). The fluorescence was acquired at Varioskan LUX Multimode Microplate Reader (Thermo Fisher Scientific). A standard curve was generated to calculate the TG concentration in each sample (ng/μL)
Replication Data for "Neuromuscular Excitation Pattern in Expert Indoor Skydivers "
No full textThis Dataset relates to EMG amplitude values obtained during Static Indoor Skydiving.
Data are embedded in a single Excel file with different sheets containing descriptive data of participants and EMG amplitudes of the investigated muscles. More information about data and methodologies can be found in the cited related publication
Replication Data for: PriSM: a Privacy-friendly Support vector Machine
No full textThe repository contains a synthetic dataset used in the experimental evaluation of the paper "PriSM: a Privacy-friendly Support vector Machine", together with the source code used for data generation, and a detailed technical report on the methodology adopted
ReData: 1884 - Daily meteorological bulletins edited by the Italian Royal Central Meteorological Office
No full textThis dataset contains the daily meteorological bulletins edited by the Italian Royal Central Meteorological Office relative to the year 1884