Islamic University of Gaza

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    The attitude of blood donors towards the use of their samples and information in biomedical research.

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    Introduction: Blood donation is important for saving human life as blood is used in surgeries, blood transfusion and for clinical use. The mission of blood banks can be extended to include medical research. The objective of the study was to determine and assess the attitude of blood donors in Gaza towards the use of their blood samples and medical data for biomedical research and the associated ethical issues. Methods: The study is cross-sectional and questionnaire-based and involved 616 blood donors from the Gaza Strip. Results: The majority of participants (86%) showed a positive attitude towards donating blood for medical research, including genetic studies. About two-thirds of participants agreed that informed consent should be taken before conducting medical research on the donated blood. A similar percentage also accepted linking of their health information to the donated blood samples. On the other hand, about 38% of participants disapproved of sharing of their data with third parties such as health insurance companies and health authorities. Finally, 10% of participants stated that their future blood donation decision would be affected if they knew that part of their donated blood was to be used in research without taking their permission. Conclusion: Blood donors in Gaza Strip have a positive attitude toward using their donated blood for medical research. More awareness is required with respect to the importance of informed consent and access to biomedical information

    Toxoplasmosis Among Pregnant and Non-Pregnant Women Attending Al-Shifa Hospital In Gaza Strip, Palestine

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    The aim our work is to study the occurrence of toxoplasmosis among pregnant and nonpregnant in Gaza strip. In cross-sectional study 164 sample was collected from pregnant and non pregnant woman attending Al-shifa hospital. Quantitative investigation of each blood sample for the levels of the specific IgG and IgM of T.gondii was done for collected sample. The overall prevalence of Toxoplasma IgG was found 33.2% and Toxoplasma. IgM was found to be 21%. The highest rate of IgG (47.4%) was in age group from 35-45 years old and IgM was (40%) in age group 25 to 35 years old. The women residing in the East Gaza city had IgG (42.2%) but in IgM was(32.1%) in the women residing in the North and Midzone. The highest rate of IgG (57.1%) and IgM (42.9%) were noticed among the employed women. In conclusion the result of this study shows a significant high seroprevalence of toxoplasmosis infection in pregnant women, and an important association with different socioeconomic factors. It is recommended that Toxoplasma gondii should applied as a routine test in Gaza and further studies to determine the reality of the definitive host infection (cats) in Gaza

    Trichonamas vaginalis Infection among Palestinian Women: Prevalence and Trends during 2000-2006

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    Aim: Trichomonas vaginalis has not been studied in Gaza and there is no available data on the prevalence of this sexually transmitted disease ( STD . The objectives of this study were to determine the prevalence of Trichomonas vaginalis ( T. vaginalis among Palestinian women attending a child and mother care center in Gaza, Palestine and to estimate the trend of prevalence over the period from 2000-2006. Materials and Methods: A cross-sectional descriptive study was conducted among 430 pregnant women attending a child and mother care center in Gaza. The clinical and gynecologic examinations were performed according to the complaints reported by each woman, especially infertility. Vaginal and cervical swabs were collected and stained with Papanicolaou ( Pap stain. Results: Out of 423 women, a total of 77 were found to be infected with T. vaginalis, for a prevalence of 18.2%. A decrease in the prevalence of T. vaginalis was observed with increasing age. A significant association between vaginal discharge and T. vaginalis infection was found ( P = 0.001 . No clear trend was noted in the prevalence of T. vaginalis over the period from 2000-2006. Conclusions: A considerable prevalence of T. vaginalis was found among pregnant women in Gaza. These results may be useful for health authorities, especially for antenatal care and protection against STDs

    Bacterial, viral and fungal genital tract infections in Palestinian pregnant women in Gaza, Palestine.

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    Back ground: Pregnant women may be at high risk to be infected with one of sexually transmitted diseases. Of the sexually transmitted infections childbearing women, Chlamydia trachomatis, Human papilloma virus (HPV) and Candida albicans are of special importante. Objective: To determine the prevalence of Chlamydia trachomatis, Human papilloma virus (HPV) and Candida albicans among pregnant women attending child and mother health center in Gaza. Methods: The present study included 423 pregnant women aged 16 to 50 years old, who were attending child and mother care center in Gaza. Their complaints included vaginal discharge and signs suggestive of acute and chronic cervicitis. Detection of Chlamydia trachomatis, Human papilloma virus (HPV) and Candida albicans was carried out using standard methods. Results: The prevalence of Chlamydia trachomatis was 35(8.3%), Human papilloma virus 55(13%) and Candida albicans 22(5.2%). Chronic cervicitis was highly correlated to HPV. The pregnant women >50 years of age were found to be the highest group infected with C.trachomatis, Human papilloma virus and C. albicans. Different results were obtained when STIs were correlated to years where some improvement in primary healthcare and privates clinics had been established as has been noticed lately in Gaza Strip, beside the easy accessible to these services for women. Conclusion: It is concluded that these sexually tranmitted infections are common among pragnant women in Gaza and that these need more investigations using advanced techniques

    Protection tools and intestinal parasitic infection among farmers in Bait-lahia, Gaza strip, Palestine

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    Protection tools are very important to avoid the transmission of intestinal parasite infection. The present study has examined the use of personal protective equipment(PPE) and its relation to intestinal parasitic infection among farmers in Bait-lahia, Gaza strip. A total of 140 stool samples were collected from the farmers between September and November 2009. Stool examinationdid not provide full protection from infection with intestinal parastic was carried out in the Department of Biology. Faculty of Science, Islamic University of Gaza. The stool samples were preserved in formalin. For stool sample examination three methods were applied: wet mount method, concentration technique and agar plate method. The results of the present study indicated that wet mount overall prevalence of intestinal parasites was 18.6% where Entameoba histolytica/dispar 6.4%. Girdia lamblia 2.1% and Ascaris lumbricoides 7%. Hymenolepis nana 1.4%. Entamoeba coli 1.4%. Chilomastix mesnili 0.7%. Low awareness regarding the use of PPE during ther work in farms was observed. However, using PPE did not provide full protection from infection with intestinal parasitic infection which manifested by some symptoms

    Diagnosis of gastrointestinal parasites among hospitalized patients attending Al-Nasser Paediatric Hospital, Gaza, Palestine.

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    Aims: The aim of this study was to determine the prevalence of the dignosed gastrointestinal parasites among hospitalized children attending the Al-Nasser Paediatric Hospital. Method: This was a cross sectional study among hospitalized children attending the Al-Nasser Paediatric Hospital. The study included 522 children during the period May 2002 to May 2003. Data collection included two methods: medical laboratory results for the examination of stool specimens and a questionnaire. wet mount using saline and Lugol's iodine and formal ether sedimentation techniques were employed in the present stydy. Results: Evidence for infection with gastrointestinal parasite was found in 176 (33.7%) of the children. The intestinal parasite were most prevalent in femals (38.8%). The most higly prevalent parasite was found to be Entamoeba histolytica/dispar (26.4%)and the monthly high prevalence of intestinal parasite was in Februrary (54.5%). The prevalence of intestinal parasites increased significantly with increasing age for both Giardia lamblia and E. histolytica/dispar. Conclusions: The study showed a high prevalence hospitalized children. Hygiene and health measures should be reviewed in the hospital where nosocomial infection cannot be excluded. The diagnostic techniques for stool specimens should be reviewed and improved. Physicians in hospitals and private clinics should be aware of intestinal parasite diseases when requesting stool specimens znd prescribing drugs

    Modified Diphasic Liver Infusion Agar: Selective Medium for the Axenic Cultivation of Entamoeba histolytica

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    A modified diphasic liver infusion agar medium was prepared manually in the laboratory in order to be used for culturing Entamoeba histolytica in vitro. This medium is a good alternative for the commercial one and has the main ingredients for the parasite's growth.  E. histolytica was successfully cultured in the present study and PCR technique was performed to confirm Entamoeba species. The growth of E. histolytica trophozoites had started in the third day post-cultivation and reached its highest rate in the seventh. Then the growth started to decrease until the trophozoites completely disappeared after 12-14 days post-cultivation. Cryopreservation using glycerol 10% ( V/V was used in order to preserve trophozoites for later cultivation

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    تحديد الانتشار المصلي لداء المقوسات بين النساء المجهضات في غزة

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    Aims: The microbial pathogen Toxoplasma gondii is commonly associated with congenital infections that are not clinically apparent. Primary T. gondii infection in the first trimester pregnancy may cause severe congenital anomalies or even foetal loss. The present study is aimed to determine the seroprevalence of toxoplasmosis among aborted women in Gaza, to study the relationship between animal rearing and the presence of Toxoplasmosis and to assess the relation of some abnormalities and infection with Toxoplasmosis. Materials and Methods: A hospital-based study was implemented to detect, describe and analyze toxoplasmosis among women with abortion attending Al-shifa hospital in Gaza strip. Quantitative investigation of each blood sample for the levels of the specific IgG and IgM of T.gondii was done from 312 aborted women. Results: The overall prevalence of Toxoplasma IgG was found 17.9% and Toxoplasma IgM was found to be 12.8%. The age of the participant woman ranged from 16 to 45 years old; 216 came from Gaza city ( 69.2% and the others reside in near villages 96 ( 30.8% . Most of the participants ( 59.0% had secondary education level. Animal breading was reported for 33.3% of the aborted women. Conclusions: Toxoplasmosis still exists and considered one of the risk factors for pregnancy miscarriage. Both IgG and IgM tests are recommended as routine tests among pregnant women in Gaza.هدفت الدراسة الحالية الى تحديد الانتشار المصلي لداء المقوسات بين النساء المجهضات في غزة. و دراسة العلاقة بين تربية الحيوانات ووجدود المرض وتقييم العلاقة لبعض الحالات غير الطبيعية والاصابة بداء المقوسات. طبقت الدراسة في المستشفى للكشف ووصف وتحليل داء المقوسات بين النساء المجهضات الذيت يترددن على مستشفىالشفاء في قطاع غزة. و قد تم اجراء فحص كمي لكل عينة دم لقياس مستويات محددة لكل من أضداد الغلوبين المناعي G و M من 312 امرأة مجهضة. و قد أظهرت النتئج أن معدل انتشار أضداد من الغلوبولينات بين 16-45 سنة. وجدت الدارسة أن 69.2% من النساء يقطن مدينة غزة, بينما 30.8% من المشاركات يقطن القرى القريبة. كذلك بينت الدراسة ان معظم المشاركات 59.0% تلقوا التعليم الثانوي. سجلت تربية الحيوانات 33.3% من النساء المجهضات. تدل نتائج هذه الدراسة أن داء المقوسات لا يزال موجودا و يعتبر أحد عوامل الخطر المسببة للاجهاض. أوصت الدارسة بأن يعتبر فحص الغلوبولينات المناعية G و M كأحد الفحوصات الروتينية للنساء الحوامل في غزة

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