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The relationship between coping strategies, resistant responses, and suggestibility in children
No full textAccording to the interrogative suggestibility model, coping strategies have an important role in determining people’s resistance to leading questions or their accepting of suggestions. In this study, a sample of 95 children aged 11–14 years were assessed for coping strategies and their effect on immediate suggestibility and Resistant Behavioral Responses (RBR) using Gudjonsson Suggestibility Scales (GSS1 and GSS2), the Children Responses Inventory for coping strategies and Raven’s Progressive Matrices. The results highlighted that task-oriented and avoidant coping strategies that involve active cognitive efforts to reduce stress and social pressure led to lower suggestibility scores and foster greater resistant responses The effect of the coping strategy, such as Logical Analysis, Problem Solving, Cognitive Avoidance and Acceptance-Resignation, was greater for the second administration of the GSS contributing to a reduction of the levels of suggestibility, Problem-solving strategy was found to be highly significant regarding resistant behavioural responses, which also increased during administration of GSS1, but they did not during the administration of GSS2. Furthermore, it emerged that with increasing age, and using more active coping strategies, the suggestibility levels tended to decrease
Edward Ravenscroft, Amanti spensierati [The Careless Lovers]. Edited by Alba Graziano, Fabio Ciambella, Bianca Del Villano, Iolanda Plescia, Valentina Rossi, and Rossana Sebellin. Roma: Bulzoni, 2024
No full textThis review presents the first Italian translation of a relatively obscure Restoration play, Edward Ravenscroft’s The Careless Lovers (1673). The peculiarity of the translation is that it is a collaborative effort by the editors, and the distinctive quality of the book is that the six translators involved (Alba Graziano, Fabio Ciambella, Bianca Del Villano, Iolanda Plescia, Valentina Rossi and Rossana Sebellin) also feature as the authors of six scholarly essays on various aspects of Restoration drama, Ravenscroft’s art, Theatre Translation and Collaborative Translation. Strikingly consistent in its translating style, complete with notes to the bilingual text, this edition amounts to a very good introduction to Restoration farce, and makes the academic reader wish for more publications in the same mould
Les traductions des œuvres de Newton en France au XVIIIe siècle
No full textTous les Savants du XVIIIe subissent le charme de Newton. Bien que l’on se rappelle principalement Madame du Châtelet comme traductrice des Principia en langue française, Diderot a aussi inséré dans ses Mémoires un Texte de Neuton en latin et en bas de page, précédé de sa traduction en français. Notre article va d’abord approfondir le contexte culturel et scientifique qui accueille en France ces découvertes révolutionnaires, pour ensuite étudier les traductions choisies et les paratextes qui les accompagnent. Ce parcours de recherche n’est certainement pas exhaustif, mais il ne manquera pas de dévoiler la porosité des domaines et des connexions possibles à travers d’autres pistes à parcourir
Synovial Fluid-Derived Extracellular Vesicles: Critical Modulators of Endothelial Cell Function
No full textIntroduction
Inflammation plays a crucial role in the pathophysiology of several musculoskeletal disorders, including Femoroacetabular Impingement (FAI) and Osteoarthritis (OA). The progression of inflammation in OA is influenced by its microenvironment, which includes endothelial cells (ECs). ECs play a key role in the abnormal angiogenesis seen in OA, increasing vascularization and contributing to inflammation. Extracellular vesicles (EVs) are nanoscale, lipid-bound particles found in the synovial fluid (SF) important in both pathological and physiological processes. They are involved in intercellular communication, mediating the transfer of numerous bioactive molecules that can impact angiogenesis and ECs activation. Even though EVs studies have been exponentially expanding over the last years, the specific effect of the SF derived EVs on ECs functions remain poorly understood.
Methods
SFs were collected from patients with FAI, categorized as having either “low” or “high” inflammation levels based on cartilage damage scores, as well from patients with OA. The levels of interleukin-6 (IL-6) in SFs were measured using an ELISA immunoassay. Ultracentrifugation (UC) was used to isolate EVs, and nanoparticle tracking analysis (NTA) to quantify them. BCA assay was used to quantify the protein concentration in the EVs population. Dot Blot for CD63 and NanoFCM for both CD9 and CD63 were used to characterize the EVs. CryoTEM was used to evaluate the isolated EVs size and morphology. The experiments in this study are limited to small extracellular vesicles (sEVs), as these were the primary focus of subsequent analysis and experimental approaches. Confocal microscopy was used to assess the uptake of SF- sEVs labelled with PKH67 by human umbilical vein endothelial cells (HUVECs). Cell viability was evaluated by Calcein AM Assay when treated with 2 and 4 μg/mL of SF-sEVs. HUVECs migratory potential when treated was investigated using Wound healing and transwell migration assays. Tube formation assay was performed on HUVECs to study the impact of SF-sEVs on angiogenesis. The expression and localization of β-catenin and VE-cadherin were analyzed using immunofluorescence. The presence of Weibel-Palade bodies (WPBs) was investigated through transmission electron microscopy (TEM). MCP-1, ICAM-1 and VCAM-1 mRNA expressions were evaluated by RT-PCR.
Results and Conclusion
Our results show increased IL-6 levels in the SF of patients, progressing from low inflammation (LI) to OA. The successful separation of EVs was confirmed through NTA, enrichment in CD63 and CD9 markers as well by the morphology and size within the typical range of sEVs. Protein concentration within the sEVs under the three experimental conditions correlated with the inflammatory state. SF-sEVs were successfully delivered into HUVECs without showing any significant toxicity. Cells showed a significant increase in their migration capacity when treated with OA-SF-sEVs. The Pro-angiogenic effects of sEVs on ECs were confirmed by the formation of capillary-like structures, with distinct variations in the density and complexity of the networks depending on the treatment. Fluorescence intensity of β-catenin and VE-cadherin were significantly reduced in cells treated with LI and OA-sEVs. TEM analysis revealed the presence of WPBs in HUVECs treated with LI-sEVs. MCP1, VCAM1 and ICAM1 mRNA levels were overexpressed.
Our preliminary data suggests that SF-sEVs influence ECs functions by modulating endothelial permeability and the inflammatory response.Introduction
Inflammation plays a crucial role in the pathophysiology of several musculoskeletal disorders, including Femoroacetabular Impingement (FAI) and Osteoarthritis (OA). The progression of inflammation in OA is influenced by its microenvironment, which includes endothelial cells (ECs). ECs play a key role in the abnormal angiogenesis seen in OA, increasing vascularization and contributing to inflammation. Extracellular vesicles (EVs) are nanoscale, lipid-bound particles found in the synovial fluid (SF) important in both pathological and physiological processes. They are involved in intercellular communication, mediating the transfer of numerous bioactive molecules that can impact angiogenesis and ECs activation. Even though EVs studies have been exponentially expanding over the last years, the specific effect of the SF derived EVs on ECs functions remain poorly understood.
Methods
SFs were collected from patients with FAI, categorized as having either “low” or “high” inflammation levels based on cartilage damage scores, as well from patients with OA. The levels of interleukin-6 (IL-6) in SFs were measured using an ELISA immunoassay. Ultracentrifugation (UC) was used to isolate EVs, and nanoparticle tracking analysis (NTA) to quantify them. BCA assay was used to quantify the protein concentration in the EVs population. Dot Blot for CD63 and NanoFCM for both CD9 and CD63 were used to characterize the EVs. CryoTEM was used to evaluate the isolated EVs size and morphology. The experiments in this study are limited to small extracellular vesicles (sEVs), as these were the primary focus of subsequent analysis and experimental approaches. Confocal microscopy was used to assess the uptake of SF- sEVs labelled with PKH67 by human umbilical vein endothelial cells (HUVECs). Cell viability was evaluated by Calcein AM Assay when treated with 2 and 4 μg/mL of SF-sEVs. HUVECs migratory potential when treated was investigated using Wound healing and transwell migration assays. Tube formation assay was performed on HUVECs to study the impact of SF-sEVs on angiogenesis. The expression and localization of β-catenin and VE-cadherin were analyzed using immunofluorescence. The presence of Weibel-Palade bodies (WPBs) was investigated through transmission electron microscopy (TEM). MCP-1, ICAM-1 and VCAM-1 mRNA expressions were evaluated by RT-PCR.
Results and Conclusion
Our results show increased IL-6 levels in the SF of patients, progressing from low inflammation (LI) to OA. The successful separation of EVs was confirmed through NTA, enrichment in CD63 and CD9 markers as well by the morphology and size within the typical range of sEVs. Protein concentration within the sEVs under the three experimental conditions correlated with the inflammatory state. SF-sEVs were successfully delivered into HUVECs without showing any significant toxicity. Cells showed a significant increase in their migration capacity when treated with OA-SF-sEVs. The Pro-angiogenic effects of sEVs on ECs were confirmed by the formation of capillary-like structures, with distinct variations in the density and complexity of the networks depending on the treatment. Fluorescence intensity of β-catenin and VE-cadherin were significantly reduced in cells treated with LI and OA-sEVs. TEM analysis revealed the presence of WPBs in HUVECs treated with LI-sEVs. MCP1, VCAM1 and ICAM1 mRNA levels were overexpressed.
Our preliminary data suggests that SF-sEVs influence ECs functions by modulating endothelial permeability and the inflammatory response
Legge 14 maggio 2025, n. 76 e tutela della salute e sicurezza sul lavoro: primi spunti per una lettura organizzativa
Get PDFIl contributo propone una riflessione sulle possibili sinergie virtuose fra la recente legge n. 76/2025 in tema di partecipazione, con particolare riferimento a quella organizzativa, e i modelli di organizzazione e di gestione ex art. 30 d.lgs. n. 81/2008, interrogandosi sull’apertura di ulteriori spazi di azione per la rappresentanza collettiva in materia di salute e sicurezza
Salute e sicurezza nei luoghi di lavoro
No full textIl saggio ricostruisce la disciplina della tutela della salute e della sciurezza sul lavoro nell'ordinamento italian
Surface sanitation against foodborne pathogens in domestic environments: efficacy of a handheld ultraviolet C light emitting diode device
Get PDFAim: Ensuring food safety is fundamental to protect public health, especially in domestic environments where food is handled daily. This two-phase study aimed to investigate the efficacy of an ultraviolet-C light emitting diode (UV-C LED) handheld lamp through in vitro disinfection tests and disinfection tests on artificially contaminated surfaces. Methods and results: The UV-C LED-based lamp efficacy was assessed at different initial microbial contamination titers and several UV doses, and both American Type Culture Collection (ATCC), foodborne, and clinical strains were considered. The UV-C LED lamp demonstrated high efficacy (log10 reduction >1 log) against the standard bacteria strains tested using a UV dose of 21.77 mJ cm-2. The greatest efficacy was achieved against E. coli (k = 0.0232) followed then by Bacillus subtilis (k = 0.0225) against which a titer of <1 CFU ml-1 was achieved with a UV dose of 15.55 and 21.77 mJ cm-2, respectively. Cladosporium spp. (k = 0.001) showed higher resistance against UV treatment, where a 50.00 ± 14.14 inactivation rate % (%IR) was achieved by applying the highest UV dose (31.1 mJ cm-2). Compared with B. subtilis, isolated L. monocytogenes 490 showed similar susceptibility (k = 0.0236), unlike isolated Listeria monocytogenes 1484 (k = 0.0146), isolated Salmonella Infantis 43 072/20 (k = 0.0126), and isolated S. Infantis 29 673/20 (k = 0.0124), which showed greater UV resistance. Considering the results obtained on the surfaces (stainless steel, polypropylene, and glass), the type of surface material influenced the susceptibility of isolated bacterial strains. However, the presence of organic matter (5% fetal bovine serum) on the treatment surface did not significantly affect device decontamination efficiency by applying a UV dose of 15.55, 21.77, and 31.1 mJ cm-2. On both stainless steel and glass, a titer <1 CFU 169 cm-2 was achieved against all the isolated bacterial strains applying a UV dose of 31.1 mJ cm-2, except with L. monocytogenes 1484. Lastly, a titer <1 CFU 169 cm-2 was never achieved on polypropylene contaminated with L. monocytogenes 1484. Conclusion: The use of a UV-C LED handheld lamp (peak wavelength 265 nm) could be an efficient disinfection method to be applied in domestic or small-scale food-processing environments, to reduce the cross contamination of food
STUDY OF PROCESSES AFFECTING AEROSOL SIZE DISTRIBUTION AND NEW PARTICLE FORMATION AT HIGH ALTITUDES
No full textAtmospheric aerosols are tiny liquid or solid particles suspended in the atmosphere,
yet they have a powerful impact threatening human health and shaping
global climate. Their effects, strongly influenced by composition, size, and number,
and their processes, including New Particle Formation (NPF), remain among
the higest uncertainties in atmospheric science, especially in the free troposphere
(FT), where observations are rare. Mountain regions, by virtue of their altitude
and remoteness, provide a unique opportunity to advance our understanding of
aerosol processes, offering valuable data that can improve climate models and air
quality predictions.
This thesis investigates almost two decades of particle number size distribution
and NPF event at Monte Cimone (CMN), a GAW global high-altitude
station in the Northern Italian Apennines (2165 m a.s.l.), representative of the
Mediterranean Basin and the Southern European free troposphere (FT). Continuous
measurements of particle size distribution using Differential/Scanning
Mobility Particle Sizer (9 nm to 560/800 nm) and Neutral Air Cluster and Ion
Spectrometers (2–40 nm for particles, 0.8–40 nm for ions) have been conducted
since 2005 within EUSAAR, ACTRIS, ACTRIS-2 and ACTRIS RI providing, to
our knowledge, one of the most extensive time series for a high-altitude site.
The study also compares recent observations in extended size range with those
from the Jungfraujoch GAW global station (JFJ) in the Swiss Alps (3454 m a.s.l.),
using the same instrumental setup, to better characterize aerosol processes and
NPF at two GAW high-altitude sites representing Southern Europe’s background
conditions.
Results show a long-term decrease in total particle concentrations at CMN of
around 96 particles/cm3 since 2005, primarily driven by a decline in accumulation
mode particles, while nucleation mode particles show an increasing tendency.
NPF events occur on average 23% of days, with the highest frequency in spring.
They are typically influenced by low condensation sink before nucleation, high
solar radiation, calm winds, dry conditions, and higher concentrations of sulfur
dioxide. Dynamic properties of stronger NPF events show moderate nucleation
rates (dNNuc/dt = 0.51 cm−3s−1), particle growth rates (GRNuc = 4.5 nm/h), and
formation rates (J10 = 0.121 cm−3s−1), with temporal trends indicating increasing
nucleation rates but slightly decreasing growth rates. The comparison with JFJ
reveals higher aerosol concentrations at CMN due to regional anthropogenic
influences from the lower altitude and transport from the Po Valley. Although JFJ
is characterized by a greater number of particles below 25 nm, CMN experiences
more frequent and intense NPF events due to stronger regional influences. These
events, classified using three complementary methods (Dal Maso, Dada, and
Aliaga), show spring and autumn peaks at both locations, with CMN exhibiting
higher formation and growth rates, particularly in spring and summer, reflecting
differences in precursor availability. The comparison of two mountain sites
reveals that NPF in the European FT can vary considerably depending on the
measurement location. Mountain can act as a source of aerosol into the FT, both
by vertically transporting primary aerosols and by creating conditions that trigger
secondary aerosol formation. The latter may be influenced by the proximity
to the FT/PBL (planetary boundary layer) interface. As NPF is considered to
contribute to a major fraction of particle number and CCN concentrations in the
FT, this new insight should be explored in greater depth and incorporated into
global models.Atmospheric aerosols are tiny liquid or solid particles suspended in the atmosphere,
yet they have a powerful impact threatening human health and shaping
global climate. Their effects, strongly influenced by composition, size, and number,
and their processes, including New Particle Formation (NPF), remain among
the higest uncertainties in atmospheric science, especially in the free troposphere
(FT), where observations are rare. Mountain regions, by virtue of their altitude
and remoteness, provide a unique opportunity to advance our understanding of
aerosol processes, offering valuable data that can improve climate models and air
quality predictions.
This thesis investigates almost two decades of particle number size distribution
and NPF event at Monte Cimone (CMN), a GAW global high-altitude
station in the Northern Italian Apennines (2165 m a.s.l.), representative of the
Mediterranean Basin and the Southern European free troposphere (FT). Continuous
measurements of particle size distribution using Differential/Scanning
Mobility Particle Sizer (9 nm to 560/800 nm) and Neutral Air Cluster and Ion
Spectrometers (2–40 nm for particles, 0.8–40 nm for ions) have been conducted
since 2005 within EUSAAR, ACTRIS, ACTRIS-2 and ACTRIS RI providing, to
our knowledge, one of the most extensive time series for a high-altitude site.
The study also compares recent observations in extended size range with those
from the Jungfraujoch GAW global station (JFJ) in the Swiss Alps (3454 m a.s.l.),
using the same instrumental setup, to better characterize aerosol processes and
NPF at two GAW high-altitude sites representing Southern Europe’s background
conditions.
Results show a long-term decrease in total particle concentrations at CMN of
around 96 particles/cm3 since 2005, primarily driven by a decline in accumulation
mode particles, while nucleation mode particles show an increasing tendency.
NPF events occur on average 23% of days, with the highest frequency in spring.
They are typically influenced by low condensation sink before nucleation, high
solar radiation, calm winds, dry conditions, and higher concentrations of sulfur
dioxide. Dynamic properties of stronger NPF events show moderate nucleation
rates (dNNuc/dt = 0.51 cm−3s−1), particle growth rates (GRNuc = 4.5 nm/h), and
formation rates (J10 = 0.121 cm−3s−1), with temporal trends indicating increasing
nucleation rates but slightly decreasing growth rates. The comparison with JFJ
reveals higher aerosol concentrations at CMN due to regional anthropogenic
influences from the lower altitude and transport from the Po Valley. Although JFJ
is characterized by a greater number of particles below 25 nm, CMN experiences
more frequent and intense NPF events due to stronger regional influences. These
events, classified using three complementary methods (Dal Maso, Dada, and
Aliaga), show spring and autumn peaks at both locations, with CMN exhibiting
higher formation and growth rates, particularly in spring and summer, reflecting
differences in precursor availability. The comparison of two mountain sites
reveals that NPF in the European FT can vary considerably depending on the
measurement location. Mountain can act as a source of aerosol into the FT, both
by vertically transporting primary aerosols and by creating conditions that trigger
secondary aerosol formation. The latter may be influenced by the proximity
to the FT/PBL (planetary boundary layer) interface. As NPF is considered to
contribute to a major fraction of particle number and CCN concentrations in the
FT, this new insight should be explored in greater depth and incorporated into
global models
