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La "Chiesa proprietaria" nel dibattito costituente: tra articolo 7 e articolo 20 della Costituzione
No full text3D-printed device for improved membrane-based extraction procedure of xenobiotics in complex matrices
No full textLa nozione di "fatto" nel contrasto teorico tra giudicati in un eclatante caso di overruling della Corte di cassazione
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Occurrence of Cystoisospora spp. and other intestinal parasites in dogs and cats with diarrhea
No full textThe occurrence of Cystoisospora spp. and other intestinal parasites in young dogs (n. 117) and cats (n. 118) with diarrhea was evaluated. Fecal samples were processed using flotation and sucrose concentration, and fecal and clinical scores were assigned to each animal. Statistical associations between the occurrence of coccidia and/or other intestinal parasites and the severity of clinical signs were evaluated. Seven dogs were infected only with Cystoisospora canis (6.8 %), 3 only with Cystoisospora ohioensis-like (3.4 %) and 1 with both (0.9 %). Forty cats (33.9 %) tested positive for coccidia, i.e. 33 (28 %) only with Cystoisospora felis, 6 (5.1 %) only with Cystoisospora rivolta, and 1 (0.8 %) with both. Other parasites recorded were Giardia spp., Ancylostomatidae, Toxocara spp. and Taeniidae. All dogs with coccidiosis had very soft feces. Watery/hemorrhagic diarrhea was detected in 4/21 (19 %) and 2/5 (40 %) cats infected with C. felis and C. rivolta, respectively. The occurrence of coccidia and Toxocara, and of hookworms and Giardia, was significantly higher (p ≤ 0.05) in cats than in dogs, and vice versa. Other significant associations found in dogs were: reduced activity and positivity to C. canis, presence of very soft feces and positivity to Ancylostomatidae. In cats, the positivity to Ancylostomatidae was significantly correlated with vomiting, dehydration, watery/hemorrhagic diarrhea. This study highlights the frequent occurrence of Cystoisospora spp. in young dogs and cats with diarrhea, with a higher incidence in cats compared to dogs. The clinical score results suggested a more pathogenic role of C. canis and C. rivolta in dogs and cats, respectively
POLYSTYRENE NANOPARTICLES IN ZEBRAFISH: THEIR ROLE IN OCULAR STRESS AND LIPIDIC METABOLIC IMBALANCE
No full textla riscossione spontanea. Definizione e modalità: autoliquidazione e liquidazione d'ufficio
No full textteoria e pratica delle varie forme di riscossione spontanea dell'ufficio, distinguendo tra autoliquidazione e liquidazione d'uffici
Comparing Immersive and Non-Immersive VR: Effects on Spatial Learning and Aesthetic Experience in Museum Settings
No full textBackground/Objectives: The use of virtual reality (VR) solutions in design has rapidly increased globally. However, it remains unclear to what extent these technologies enhance people’s cognitive abilities. Understanding the impact of such technologies is essential for assessing their validity and effectiveness. In this controlled study, we investigated how HMD and non-immersive VR museum environments affect the ability to learn about the environment and the perception of the experience. Methods: A total of 87 college students (46 females) were randomly assigned to either HMD or non-immersive VR museum environments. Subsequently, they had to answer questions related to the sense of immersion and pleasantness of the museum experience, as well as their willingness to repeat similar museum experiences. Results: The results indicated that the HMD setting was preferred for its greater sense of immersion, pleasantness, and intention to repeat a similar experience. Conclusions: The data are discussed in the context of fostering appreciation and promoting the preservation of cultural heritage
Paslahepevirus balyani in Suidae coming from the Monti Sibillini National Park (2020-2024)
No full textPaslahepevirus balayani or hepatitis E virus (HEV) represents one the most diffused viral zoonotic foodborne pathogens in Europe, and genotype 3 results one of the most identified in Italy among Suidae. For this reason, the aim of this work was to carry out biomolecular and phylogenetic evaluations of the persistence and evolution of HEV-3 in wild boars (Sus scrofa) and domestic swines (Sus scrofa domesticus) from the Monti Sibillini National Park (Ascoli Piceno province) in the period between 2020 and 2024. More in detail, from December 2020 to January 2024, the sampling of n. 1054 Suidae was performed and two aliquots (15±2 g) of tissue were taken from each of them: one from the liver and one from muscle tissue (diaphragm pillar) obtained by plucks conferred at the slaughterhouse level in Ascoli Piceno (EU Reg. No. 624/2019, No. 625/2017, and R.L. Marche No. 13/2012). In the tested population, 818/1054 (77.61%) were wild boars and 236/1054 (22.39%) were domestic pigs raised with extensive methods for domestic consumption. Tissues followed the same standardized laboratory procedures: homogenization, RNA extraction by the Trizol LS method, and finally biomolecular tests: real time RT-qPCR and nested RT-PCR of the codifying regions ORF-1, ORF-2, and ORF-3 of HEV. Sequencing of the positive ones was followed by: nucleotide similarity, alignment and evolutionary analysis (phylogenetic tree construction using Neighboring-Joining) using MEGA X software. Finally, they have been deposited and published on the GenBank platform. The results showed that HEV RNA was amplified by the liver of 114/1054 (10.81%) (102 GE/ μL) subjects tested, and 26/114 (22.81%) of them also presented positive muscle tissues too. None of them harbored HEV RNA in the muscle only. From a comparative and species point of view, wild boars presented a prevalence of 72/114 (63.16%), and domestic pigs of 42/114 (36.84%). The ORF-1 region was mostly amplified by 97/114 (85.08%) (103 GE/μL) of Suidae, while ORF-2 (102 GE/μL) only in the wild boar populations 72/114 (63.16%). In the period between 2020-2024, the loaded sequences on the GenBank platform were as follow: MN20202101, MN20202102, MN20202103, MN20202104, OR933697, OR93398, MN2020210201, MN2020210208, MN2020210212, ON364349, ON364350, ON364351, ON364352. In all positive cases, nucleotide similarity (over 98.0% average), alignments and phylogenetic analyses have demonstrated the involvement of HEV-3c during the five years 2020-2024. The radicalization of the presence and persistence of HEV-3c (from 2020 to 2024) in the Ascoli Piceno province confirms that the Suidae have a fundamental role as reservoirs in urban and wild cycles. The sharing of areas between extensive domestic pigs’ farms and the coexistence with wild boars within the Monti Sibillini National Park have allowed possible cross species -infection, as supported by the analysis of the discovered sequences. It is therefore clear that biomolecular surveillance on the territory has a fundamental role in terms of food safety and prevention for the final consumer