743 research outputs found
Adherence to guidelines strongly improves reproducibility of brachial artery flow-mediated dilation.
No full textBACKGROUND: Brachial artery FMD is widely used as a non-invasive measure of endothelial function. Adherence to expert guidelines is believed to be of vital importance to obtain reproducible measurements. We conducted a systematic review of studies reporting on the reproducibility of the FMD in order to determine the relation between adherence to current expert guidelines for FMD measurement and its reproducibility. METHODS: Medline-database was searched through July 2015 and 458 records were screened for FMD reproducibility studies reporting the mean difference and variance of repeated FMD measurements. An adherence score was assigned to each of the included studies based on reported adherence to published guidelines on the assessment of brachial artery FMD. A Typical Error Estimate (TEE) of the FMD was calculated for each included study. The relation between the FMD TEE and the adherence score was investigated by means of Pearson correlation coefficients and multiple linear regression analysis. RESULTS: Twenty-seven studies involving 48 study groups and 1537 subjects were included in the analyses. The adherence score ranged from 2.4 to 9.2 (out of a maximum of 10) and was strongly and inversely correlated with FMD TEE (adjusted R(2) = 0.36, P < 0.01). Use of automated edge-detection software, continuous diameter measurement, true peak diameter for %FMD calculation, a stereostatic probe holder, and higher age emerged as factors associated with a lower FMD TEE. CONCLUSIONS: These data demonstrate that adherence to current expert consensus guidelines and applying contemporary techniques for measuring brachial artery FMD decreases its measurement error
Adsorption performance of g-C3N4/graphene, and MIL-101(Fe)/graphene for the removal of pharmaceutical contaminants: a molecular dynamics simulation study
No full textAbstract The rising presence of drug-related contaminants in water sources is a major environmental and public health concern. Several studies have addressed the hazardous influence of these pollutants on the lives of over 400 million people worldwide. In this study, we used molecular dynamics simulations to evaluate the efficacy of two promising composite materials for the removal of pharmaceutical contaminants by using the adsorption technique. Graphitic carbon nitride/graphene (g-C3N4/graphene) and metal-organic framework (MIL-101(Fe))/graphene have been simulated for the first time for the removal of three of the most common pollutants (acetaminophen (AC), caffeine (CAF), and sulfamethoxazole (SMZ)). The nanocomposite structure has been created and optimized using the geometry optimization task in the DFTB Modules in the Amsterdam Modeling Suite. We summarized the condition of the essential parameters (Temperature, pressure, and density) of the simulation box during the MD-simulation to ensure the accuracy of our MD-simulation results. The adsorption process, van der Waals interactions, and the adsorption capacity have been calculated by using the Reactive Forcefield (ReaxFF) software. We found that the combination of MIL-101(Fe)/graphene had a higher adsorption capacity for the removal of pharmaceutical contaminants than g-C3N4/graphene. At 40 Picosecond (Ps), 80 molecules of each pharmaceutical contaminants (AC, CAF and SMZ) have been adsorbed by MIL-101(Fe)/graphene with higher exothermic energy equated to (−1174, −1630, and − 2347) MJ/mol respectively. While for g-C3N4/graphene at 40 Ps, 70 molecules of each pharmaceutical contaminants have been adsorbed with exothermic energy equated to (−924, −966, and − 1268) MJ/mol respectively. Also, our results showed that the combination of g-C₃N₄/graphene and MIL-101(Fe)/graphene both have remarkable properties that make them effective at resisting surface clogging. Finally, the results showed that the adsorption kinetics followed a pseudo-first order model, while the adsorption isotherms for AC, CAF and SMZ adhered to Freundlich model
Salusins protect neonatal rat cardiomyocytes from serum deprivation-induced cell death through upregulation of GRP78
No full textSalusin-alpha and salusin-beta are newly identified bioactive peptides with hemodynamic and mitogenic activities. Recent studies have shown that salusins improve calcium uptake and protein synthesis in neonatal rat cardiomyocytes, suggesting that salusins may be regulatory factors for myocardial growth and hypertrophy. In this study, we investigated whether salusins improve the survival of cardiomyocytes after serum deprivation. Cultured neonatal cardiomyocytes were treated with or without salusins (salusin-alpha or salusin-beta) at a concentration range of 10(-10) to 10(-8) mol/L for 24 h under serum deprivation conditions. Cardiomyocytes viability was determined by 3-(4.5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazonium bromide assay. Cell death or apoptosis rate was identified by flow cytometry analysis. Compared to serum deprivation-only groups, cardiomyocyte viability was significantly increased in salusin-a or salusin-beta groups. Cell death rate was decreased after administration of 10(-8) mol/L salusin-alpha or salusin-beta. Salusin-beta was able to decrease the apoptotic rate. Salusins also increased the expression of cardiomyocyte glucose-regulated protein 78 (GRP78) as estimated by Western blot. Furthermore, antisense oligodeoxynucleotide specifically against GRP78 attenuated or abrogated antiapoptosis or survival effects of salusin-beta. These findings suggest that salusin-a and salusin-beta may be a potential survival factor against serum deprivation-induced myocardial cell death and that this cardioprotective effect may involve an upregulation of GRP78 expression in card iomyocytes.Cardiac & Cardiovascular SystemsPharmacology & PharmacySCI(E)5ARTICLE241-464
Stable carbon isotopes and prehistoric diets in the Eastern Cape Province, South Africa
No full textIncludes bibliographical references.The research reported in this thesis involves the measurement of stable carbon isotope ratios in human bone collagen as a means of reconstructing prehistoric diets. The sample population includes 67 skeletons of hunter-gatherers, pastoralists and agriculturalists from the Holocene of the Eastern Cape Province, South Africa. The aims of the thesis include the testing, through direct quantitative measurements, of the validity of archaeological conclusions about prehistoric human behaviour in the Eastern Cape. Secondly, the usefulness and applicability of the 13c tracer technique is demonstrated in what is arguable the most complex situation an archaeologist is likely to encounter. The natural environment included c3 and c4 plants, browsing and grazing ungulates, and a marine component - all subject to environmental change over the period under study - while the cultural environment included three different subsistence systems plus transition stages between them. A third, or subsidiary goal, was to test whether burial practices can be correlated with subsistence economies in this situation - that is, whether ritual and dietary behaviour formed part of some larger cultural whole such as "pastoralists" - in order to be able to assign individuals to socio-economic groups on the basis of burial pattern. The results of the laboratory analysis realize these goals with varying degrees of success and with important consequences for the archaeologist
Evaluation of the predictive accuracy of MRI-detected erosions in hand and foot joints in patients with undifferentiated arthritis
No full textPathophysiology and treatment of rheumatic disease
Depression and anxiety associate with less remission after 1 year in rheumatoid arthritis
No full textPathophysiology and treatment of rheumatic disease
Welfare of farmed fish : towards a sustainable development of European aquaculture
No full textThe European project FASTFISH has developed a fish behaviour monitoring system for aquaculture to ensure that fish production continues to be legitimised by society and remains competitive in production performance. Adoption of the monitoring system in society is a political process of choices and strategies, requiring experience and capacity from all stakeholders. The main challenge of FASTFISH is to combine learning processes as well as accountability for retailers and non-governmental organisations by establishing a platform facilitating stakeholder arrangement
Contribution of tenosynovitis of small joints to the symptom morning stiffness in patients presenting with undifferentiated and rheumatoid arthritis
No full textObjective: Morning stiffness (MS) is characteristic of rheumatoid arthritis (RA). Despite its association with functional disability, the extent to which local inflammatory processes contribute to this symptom is unknown. Magnetic resonance imaging (MRI)-detected tenosynovitis of small joints is recognized as an early feature of RA, which is also associated with functional impairments. It has been proposed that tenosynovitis contributes to MS. Therefore, we assessed the relationship between MS and MRI-detected inflammation, in particular tenosynovitis. Method: In total, 286 consecutive patients newly presenting with undifferentiated arthritis and RA underwent contrast-enhanced 1.5 T MRI of (2–5) metacarpophalangeal, wrist, and (1–5) metatarsophalangeal joints. Scans were scored for tenosynovitis according to Haavardsholm, and for synovitis by Rheumatoid Arthritis Magnetic Resonance Imaging Scoring (RAMRIS). MS was dichotomized as ≥ 60 min or not. Associations between MS and tenosynovitis/synovitis were tested with logistic regression, data were categorized (solitary or simultaneous presence of synovitis/tenosynovitis), and the presence of an additive interaction was assessed. Results: MS was present in 40% of patients. Tenosynovitis was more often present in patients with MS than without MS [80% vs 65%, odds ratio (OR) 2.11, 95% confidence interval (1.21;3.69)]. Synovitis was more often present in patients with MS [58% vs 44%, OR 1.79 (1.11;2.91)]. In categorized analyses, concurrent synovitis and tenosynovitis had the largest association [OR 2.43 (1.30;4.54)], in contrast to solitary synovitis [OR 0.85 (0.21;3.47)]. The additive interaction was non-significant. The variance explained in all analyses was small (range 4–5%). Conclusion: Tenosynovitis, combined with synovitis, at small joints is associated with MS and contributes to the pathophysiology of MS.</p
Improving the feasibility of MRI in clinically suspect arthralgia for prediction of rheumatoid arthritis by omitting scanning of the feet
No full textObjectives. The use of MR-imaging is recommended for the early detection of RA. Next to the small joints of the hands, foot-joints are often involved. Therefore, imaging inflammation of the feet in addition to hands may be informative, but prolongs scan-time and leads to additional costs. We studied the value of MRI of the feet alone and complementary to MRI of the hands in patients with clinically suspect arthralgia (CSA).Methods. 357 consecutively included CSA patients underwent contrast-enhanced 1.5 T-MRI of hand (MCP2-5 and wrist) and foot (MTP1-5) joints at baseline. Scans were scored for synovitis, osteitis and tenosynovitis. After 1 year follow-up, the development of clinically apparent inflammatory arthritis (IA) was studied. Cox regression was performed and test characteristics were evaluated. Sensitivity analyses were performed for the outcome RA-development (2010-criteria).Results. MRI-detected tenosynovitis of the feet was associated with IA-development, independently from synovitis and osteitis hazard ratio (HR) (95%CI) 4.75 (2.38; 9.49), and independently from ACPA and CRP, HR 3.13 (1.48; 6.64). From all CSA patients, 11% had inflammation in hands and feet, 29% only in hands and 3% only in feet. In line with this finding, the addition of MRI-feet to MRI-hands did not increase the predictive accuracy; the sensitivity remained 77%, while the specificity decreased from 66% to 62%. Sensitivity analyses with RA development as outcome showed similar results.Conclusion. Tenosynovitis at the forefeet in CSA predicted IA and RA development. Addition of foot MRI to hand MRI did not increase the accuracy. Foot MRI can be omitted to reduce scan time and costs and increase the feasibility.Pathophysiology and treatment of rheumatic disease
The use of MRI-detected synovitis to determine the number of involved joints for the 2010 ACR/EULAR classification criteria for Rheumatoid Arthritis - is it of additional benefit?
No full textPathophysiology and treatment of rheumatic disease
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