1,880,618 research outputs found
Clustering and dynamics of cytochrome bd-I complexes in the Escherichia coli plasma membrane in vivo.
No full textThe cytochrome bd-I complex of Escherichia coli is a respiratory terminal oxidase and an integral component of the cytoplasmic membrane. As with other respiratory components, the organization and dynamics of this complex in living membranes is unknown. We set out to visualize the distribution and dynamics of this complex in vivo. By exchanging cydB for cydB-gfpgcn4 on the E. coli chromosome, we produced a strain (YTL01) that expresses functional GFP-tagged cytochrome bd-I terminal oxidase complexes under wild-type genetic control. We imaged live YTL01 cells using video-rate epifluorescence and total internal reflection fluorescence (TIRF) microscopy in combination with fluorescence recovery after photobleaching (FRAP) and saw mobile spots of GFP fluorescence in plasma membranes. Numbers of GFP molecules per spot were quantified by step-wise photobleaching giving a broad distribution with a mean of approximately 76, indicating that cytochrome bd-I is concentrated in mobile patches in the E. coli plasma membrane. We hypothesize that respiration occurs in mobile membrane patches which we call 'respirazones'
Abteilung 1 : Bd. 1 - Zweiter Halbbandl92.
No full textAbteilung 3 t Bd. 1 ! 1929. Bd. 2 : 1930. EdA3 : 1930Sonderausgabe S 4 ! 193
Capteur CentoNewton-Bd' - ajustement
No full textCe document décrit l'ajustement de la version Bd' du capteur de force CentoNewton. Malgré les corrections déjà introduites, l'itération Bd a toujours un problème d'ajustement de l'offset, en raison du mauvais appariement des résistances d'ajustement grossier discret de l'offset (1±, 2±, et 3±), et de l'impossibilité de mesurer des offsets négatifs inhérente au signal amplifié. De ce fait, une révision mineure (Bd') a été faite, avec 1) un meilleur appariement des coupes et 2) un plus grand décalage positif intentionnel des valeurs d'offset du circuit combiné avec un faible gain initial, pour éviter / minimiser la saturation de l'offset initial. This document describes the trimming of version Bd' of the CentoNewton force sensor. In spite of previously-introduced corrections adjustment of the offset of version Bd still turned out to be problematic, due to the poor matching of the digital rough offset trimming resistors. combined with the impossibility to measure negative offset, which is inherent to the trimming of the amplified signal. Therefore, a minor revision (Bd') was made, with 1) better matching of the digital cuts and 2) an larger intentional positive offset shift of the circuit, combined with a low initial gain, in order to avoid / minimise the saturation of the initial offset.LPMPrésenté / publié en partie à IMAPS-PL Rzeszów-Czarna, Infoscience #20510
Hegel and Deleuze: immanence and otherness
No full textThe thesis critically analyses the dominant foundationalist tendency of modern philosophy, with special reference to the sophisticated antifoundationalist critiques of foundationalism formulated by G.W.F. Hegel and Gilles Deleuze.
It begins by outlining a general methodological aspect of foundationalism, regarding the necessity of radical self-critique in philosophy, which directly connects contemporary thought with Cartesianism, via classical German philosophy.
In the philosophies of Kant, Fichte and Schelling, this self-critical project is transformed: they undertake to show that reason can, by examining itself, give an account of experience that is systematic, or consistent with itself. However, each of these thinkers fails to accomplish this, and indeed, the commitment to a priori foundations is itself undermined in Schelling's work; where a philosophical crisis of meaning (a 'trauma of reason', philosophical nihilism) emerges.
Deleuze and Hegel's contrasting critiques of foundationalism, and their positive reconstructions of the standpoint of philosophy, are then interpreted as non-foundationalist attempts to overcome this internal crisis of foundationalist thought as inadvertently exposed by Schelling. Both criticise certain subjective presuppositions common to foundationalist philosophies, which they consider constitute a dogmatic 'image' of philosophy, a kind of transcendental illusion that is the guiding force behind foundationalism. Both also aim to replace this with a genuinely philosophical image.
The thesis provides an original historical contextualisation of Deleuze's thought in relation to German Idealism, and Schelling in particular, with whom, it is argued, Deleuze has much in common. Deleuze's conception of pure difference is treated in this regard as a kind of 'absolute knowledge'. This contextualisation also allows the sometimes crudely understood antipathy between Hegel and Deleuze to be addressed in a more penetrating fashion, which shows that they have more in common in terms of their critical orientation than is usually supposed.
The thesis concludes with a critical comparison of these thinkers, which argues that, although both succeed in their own terms, in relation to a criterion of self-consistency, Hegel's philosophy offers a more satisfactory treatment of the ontological and historical conditions of philosophical activity
Dossier : Maîtres de la BD européenne : Ressource du site Maîtres de la BD européenne
No full textTrois dossiers thématiques permettent d’approfondir les grandes tendances de la BD européenne, les principales représentations de héros et, enfin, les techniques de la bande dessinée
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Maîtres de la BD européenne : Le site complet
No full textLe site propose une découverte des grands maîtres de la BD européenne à travers une centaine de planches. Trois dossiers thématiques permettent d’approfondir les grandes tendances de la BD européenne, les principales représentations de héros et enfin, les techniques de la bande dessinée. Plusieurs planches peuvent être explorées à la loupe, comme M. Vertpré et M elle d’Espagnac de Töpffer ou L’enfant penchée de Schuiten et Peeters. Des repères sous forme de biographies, un glossaire et des pistes bibliographiques permettent à l’internaute de prolonger sa visite
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