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    Morphophysiological markers apt to individuate different susceptibility of pear rootstocks cultivated in 'in vitro' iron-deficient conditions

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    In many deciduous fruit trees species lime-induced chlorosis represents a serious economical damage. It may be overcome by agronomic strategies, as the use of tolerant rootstocks, valid alternative to reduce expensive iron-chelate fertilisation and chemical environmental impact. Since few years in vitro culture technique is applied to test rootstocks for iron chlorosis susceptibility. Micropropagated shoots were used for screening of quince rootstocks MA and BA 29, and pear cv. Conference under stress conditions on MS medium supplemented with different concentrations of FeNaEDTA, 100%=0.1 mM Fe (FeN) and 15%=0.015 mM (Fe15), and/or potassium bicarbonate, 0 mM (FeN) and 1 mM KHCO3 (FeNC and Fe15C). After 24 days, substrate pH, shoot growth markers, total leaf iron and photosynthetic pigment contents were determined. The medium pH reduction resulted similar among genotypes but different among treatments. Iron deficiency and high bicarbonate concentration induced different growth performances among the genotypes, reflecting a different sensibility and plastic adapation to the induced iron chlorosis. Total leaf iron content decreased in all genotypes, when iron deficiency and bicarbonate concentration increasing onto the medium, although pear cultivar showed more slight reduction. Photosynthetic pigments, analysed by HPLC, decreased in all type of stress, and the lowest levels were detected in Fe15C treatment, except for cv. Conference. A similar trend was exhibited by carotenoids, in particular by Violaxanthin. Our preliminary data confirm the potential of in vitro culture as a rapid screening method. The choice of markers and the result interpretation will be deeply discussed in the paper

    Differential responses in pear and quince genotypes induced by Fe deficiency and bicarbonate

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    Most of the studies carried out on Fe deficiency condition in arboreous plants have been performed, with the exception of those carried out on plants grown in the field, in hydroponic culture utilizing a total iron depletion growth condition.This can cause great stress to plants. By introducing Fe deficiency induced by the presence of bicarbonate,we found significant differences between Pyrus communis L. cv. Conference and Cydonia oblonga Mill. BA29 and MA clones,characterized by different levels of tolerance to chlorosis. Pigment content and the main protein–pigment complexes were investigated by HPLC and protein gel blot analysis, respectively. While similar changes in the structural organization of photosystems(PSs)were observed in both species under Fe deficiency, a different reorganization of the photosynthetic apparatus was found in the presence of bicarbonate between tolerant and susceptible genotypes, in agreement with the photosynthetic electron transport rate measured in isolated thylakoids. In order to characterize the intrinsic factors determining the efficiency of iron uptake in a tolerant genotype, the main mechanisms induced by Fe deficiency in Strategy I species, such as Fe3+ -chelate reductase (EC1.16.1.7) and H+-ATPase (EC 3.6.3.6) activities, were also investigated. We demonstrate that physiological and biochemical root responses in quince and pear are differentially affected by iron starvation and bicarbonate supply, and we show a high correlation between tolerance and Strategy I activation

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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