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Identification of morphological, biochemical and physiological parameters useful to characterize nutritional stress status in arboreous species differently tolerant to chlorosis
L'impiego del " push in " nel trattamento delle stenosi post traumatiche dell'uretra posteriore
Morphophysiological markers apt to individuate different susceptibility of pear rootstocks cultivated in 'in vitro' iron-deficient conditions
In many deciduous fruit trees species lime-induced chlorosis represents a serious economical damage. It may be overcome by agronomic strategies, as the use of tolerant rootstocks, valid alternative to reduce expensive iron-chelate fertilisation and chemical environmental impact. Since few years in vitro culture technique is applied to test rootstocks for iron chlorosis susceptibility. Micropropagated shoots were used for screening of quince rootstocks MA and BA 29, and pear cv. Conference under stress conditions on MS medium supplemented with different concentrations of FeNaEDTA, 100%=0.1 mM Fe (FeN) and 15%=0.015 mM (Fe15), and/or potassium bicarbonate, 0 mM (FeN) and 1 mM KHCO3 (FeNC and Fe15C). After 24 days, substrate pH, shoot growth markers, total leaf iron and photosynthetic pigment contents were determined. The medium pH reduction resulted similar among genotypes but different among treatments. Iron deficiency and high bicarbonate concentration induced different growth performances among the genotypes, reflecting a different sensibility and plastic adapation to the induced iron chlorosis. Total leaf iron content decreased in all genotypes, when iron deficiency and bicarbonate concentration increasing onto the medium, although pear cultivar showed more slight reduction. Photosynthetic pigments, analysed by HPLC, decreased in all type of stress, and the lowest levels were detected in Fe15C treatment, except for cv. Conference. A similar trend was exhibited by carotenoids, in particular by Violaxanthin.
Our preliminary data confirm the potential of in vitro culture as a rapid screening method. The choice of markers and the result interpretation will be deeply discussed in the paper
L'affondamento intercavernoso dell'uretra bulbo perineale nel trattamento dell'incontinenza urinaria maschile. Rivista Medica Trentina XV,109, 1977.
Differential responses in pear and quince genotypes induced by Fe deficiency and bicarbonate
Most of the studies carried out on Fe deficiency condition in arboreous plants have been performed, with the exception of those carried out on plants grown in the field, in hydroponic culture utilizing a total iron depletion growth condition.This can cause great stress to plants. By introducing Fe deficiency induced by the presence of
bicarbonate,we found significant differences between Pyrus communis L. cv.
Conference and Cydonia oblonga Mill. BA29 and MA clones,characterized by
different levels of tolerance to chlorosis. Pigment content and the main protein–pigment complexes were investigated by
HPLC and protein gel blot analysis, respectively. While similar changes in the
structural organization of photosystems(PSs)were observed in both species under Fe deficiency, a different reorganization of the photosynthetic apparatus was found in the presence of bicarbonate between tolerant and susceptible genotypes, in
agreement with the photosynthetic electron transport rate measured in isolated thylakoids. In order to characterize the intrinsic factors determining the efficiency of iron
uptake in a tolerant genotype, the main mechanisms induced by Fe deficiency in
Strategy I species, such as Fe3+ -chelate reductase (EC1.16.1.7) and H+-ATPase
(EC 3.6.3.6) activities, were also investigated. We demonstrate that physiological and biochemical root responses in quince and pear are differentially affected by iron
starvation and bicarbonate supply, and we show a high correlation between
tolerance and Strategy I activation
Going Beyond Counting First Authors in Author Co-citation Analysis
The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Identification of morphological, biochemical and physiological parameters useful to characterize nutritional stress status in arboreous species differently tolerant to chlorosis
Tomato fer mutation, by altering the root iron acquisition efficiency, induces H2O2 accumulation at root and leaf level
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