1,721,081 research outputs found
improvising in the community of philosophical inquiry: a way to learn to inhabit uncertainty|improvisar na comunidade de investigação filosófica: uma forma de aprender a habitar a incerteza|improvisar en la comunidad de investigación filosófica: una forma de aprender a habitar la incertidumbre
No full textThis theoretical contribution proposes an open reflection on uncertainty, present in contemporary social and educational discussions, and on the value that learning to inhabit such “uncertainties” can have for the well-being of individuals and communities. Perceiving different alternatives before making a choice, finding yourself lost when faced with the ambiguity of some information, and feeling disorientated when faced with complexity, are all characteristics of this elusive “uncertainty” (Barreneche & Santi, 2022). Uncertainty can be perceived negatively if it provokes fear, worry, anxiety, perception of vulnerability (Hillen et al., 2017) or in a constructive way if it appears as a vital engine for research (Tauritz, 2019). Community of philosophical inquiry (CPI) – as conceptualized and promoted in the Philosophy for Children (P4C) curriculum - becomes a “safe creativity environment” (Weinstein, 2016), in which to learn to inhabit uncertainty. A CPI that improvises (Zorzi & Santi, 2023), develops and makes conscious dispositions towards uncertainty, learning to see alternatives as a resource, ambiguities as creative horizons of meaning, and complexity as a collective performance to be experienced. Six improvisational dimensions – (1) respecting diversities, (2) trusting possibilities, (3) building a sense of community, (4) opening attitude towards changes, (5) explorative attitude, (6) creative passion, emerged from other previous researches (Zorzi, Camedda, Santi, 2019), are proposed to orient facilitators’ and CoI's training, dialoguing with the “nine recurring moments of disequilibrium” suggested by Karin Murris (2008)
Improvvisare per imparare a sentirci irripetibili: “unrepeatable meshworks”, la proposta di un percorso di Ricerca-Formazione
Full text link“Improvising to learn to feel ourselves unrepeatable”: “ourselves” is a personal pronoun that embodies at the same time the reflexivity addressed to each one (me?) and that addressed to the plural (we?). It is that “be-there” that recalls the being that is there and, at the same time, our co-originally being-with-others. An invitation (a provocation?) is therefore open to all and each, no one excluded. This contribution aims to offer a design-pedagogical reflection that focuses on the concept of being unrepeatable, its connection with improvisation, and the integrating background of equity and well-being, at school (in the world?). A teachers professional development research proposal - here presented in the design phase - based on an original perspective that focuses the attention on the meshwork, to enhance the unrepeatable interweaving that characterize our lives and which further underline our uniqueness and diversities
Documentare per creare reti: narrative digitali attraverso lo storytelling
No full textIl Sistema integrato dell’educazione e della formazione 0-6, sottolinea la funzione educativa primaria delle famiglie, incoraggiando il loro coinvolgimento attivo nella comunità scolastica. I dati di una ricerca project-based che ha visto il coinvolgimento di 184 genitori ha inteso dimostrare l’efficacia della documentazione attraverso narrative digitali. La tecnica del Digital Storytelling è stata usata per definire nuove strategie ed azioni, per innovare le pratiche di comunicazione scuola-famiglia e attivare processi di partecipazione attiva
Proteasome inhibitor PS-341 blocks cell growth and induces apoptosis in anaplastic large cell lymphoma cell lines
No full textBlockade of the proteasome degradation pathway has emerged as a novel strategy to induce apoptosis in several tumor models, and PS-341 (Velcade) is among the newest compounds that show proteasome inhibitor activity that strictly correlates with antitumor effect. In this study, we investigated the antiproliferative activity of PS-341 in Anaplastic Large Cell Lymphomas (ALCLs), a high grade non-Hodgkin lymphoma characterized by the presence of specific chromosomal translocations involving the anaplastic lymphoma kinase (ALK) gene. Here we report that when administered for increasing time intervals PS-341 potently inhibited the growth of both ALK-positive (KARPAS, SUDHL1, SR786) and -negative (FE-PD) ALCL cell lines, irrespective of ALK-fusion proteins status and activity. The IC50s, as assayed by MTT following 24 h exposure, were 4.5-6 nM for SUDHL1, SR786 cells and the ALK-negative cell line FE-PD, whereas 17.8 nM for KARPAS. These cells resulted somehow more resistant to drug treatment, as time-dependent cleavage of PARP protein observed between 8 and 12 hours in SR786, SUDHL1 and FE-PD with 20 nM PS341, occurred only at higher concentrations (100 nM) in KARPAS cells. Because of the PS-341 pro-apoptotic activity, we assessed the activation of apoptotic caspase proteases and found that effector caspase-3 was strongly induced between 4 and 8 hours in ALCL lymphoma cells, and preceded the activation of caspase-8 and -9. Indeed, the caspase-8 inhibitor IETD-CHO prevented the formation of the active form of caspase-8 in the presence of PS-341, while it failed to block the procaspase-3 processing and PARP cleavage under the same experimental conditions. In contrast, the pan-caspase inhibitor ZVAD-fmk and the specific caspase-3 peptide inhibitor DEVD-CHO prevented both caspase-3 activation and PARP cleavage in all the cell lines. As expected, activation of caspases and mitochondrial cytochrome-c release was delayed in KARPAS cells, as well as up-regulation of the two cell-cycle inhibitors p27Kip and p21WAF. In contrast, the anti-apoptotic protein Mcl-1 was overexpressed in KARPAS cells and only slightly downregulated in the presence of PS-341, despite the potent inhibition of proteasome activity (90%) measured by fluorometric assay. Together, these data demonstrate the efficacy of PS-341 at inducing apoptosis in ALCL lymphoma cells, and suggest that induction of apoptosis may depend not solely on the inhibition of the 20S Proteasome, but also on the specific molecular profile of the individual cell line
Learning Revision Skills through Cognitive Apprenticeship: A didactic Experience in a Collaborative Context
Full text linkAlthough writing takes on fundamental role in people’s lives and education, cognitivism and socio-cultural constructivism studies have shown that teach-ing practices do not often stimulate and do not adequately support pupils in developing a mature approach to written composition. Specifically, one of the typical problems of apprentice writers, is represented by the revision process, traditionally understood as rereading and correction of spelling er-rors and embellishment of the text at the end of writing. However, according to scholars of the psychology of written composition, it is possible to help apprentices develop a mature approach to revision and complex revision skills through specific educational programs and teaching methodologies. Therefore, present paper aims to propose the use of cognitive apprenticeship in a collaborative context and the use of procedural facilitations as an effective methodological choice to stimulate the acquisition of a mature ap-proach to writing and, in particular, to the revision process in elementary school pupils
Il dis-orientamento come navigazione nell’incertezza: una ricerca con adolescenti in comunità di indagine filosofica
Full text linkLe Linee Guida del MIM del 2022 presentano l’orientamento nella sua dimensione individuale, focalizzando l’attenzione sul ruolo del merito personale nel successo formativo e professionale. In contrapposizione a questa prospettiva individualistica, si intende in questa ricerca proporre un'espressione sociale di orientamento, elaborando didatticamente una proposta che possa insegnare come affrontare comunitariamente un problema o una domanda, sostare insieme nell’incertezza e cercare collettivamente una possibile risposta.
Lo studio di questa dimensione comunitaria invita l'orientamento scolastico ad assumere uno sguardo critico sulla vita in società complesse, tempi cambiati e futuri incerti. Emerge dunque l’esigenza di una didattica orientante, che può trovare nella metodologia della Philosophy for Children (P4C) un modo di proporre e percorrere comunitariamente un problema (Barreneche & Santi, 2022). Questa ricerca esplorativa si presenta quindi come una proposta che, tramite l'esperienza educativa di P4C in aula con gli adolescenti, dimostra il suo valore per l'orientamento concepito collettivamente e che rivela il dis-orientamento come un carico condiviso all’interno di una comunità di indagine; aspetti entrambe fondamentali in contesti di società incerte
EFFETTO ANTITUMORALE DELLA COMBINAZIONE BORTEZOMIB E FLAVOPIRIDOLO NEL TRATTAMENTO DI CELLULE DI ALCL IN VITRO
No full textBortezomib è il capostipite di una nuova classe di farmaci
nota come inibitori del proteosoma con potente attività proapoptotica
e antiproliferativa. Per la sua attività antitumorale
Bortezomib è attualmente impiegato in diversi trials clinici
come agente singolo od in combinazione con chemioterapici
convenzionali e sinergismo è stato osservato se somministrato
in combinazione con modificatori della trascrizione cellulare.
Tra questi, Flavopiridolo, un inibitore delle chinasi ciclina
dipendenti (cdk), ha già dimostrato attività antitumorale
sinergica in combinazione con Bortezomib in cellule di leucemia,
CML. Lo scopo di questo studio è stato quello di analizzare
l’interazione tra Flavopiridolo e Bortezomib, su quattro
linee cellulari di linfoma anaplastico a grandi cellule
(ALCL), con espressione o meno della chinasi oncogena NPMALK.
Per valutare l’effetto antiproliferativo sinergico, saggi in
vitro di citotossicità sono stati condotti su linee di ALCL trattate
per tempi variabili con concentrazioni nanomolari di flavopiridolo
(50-200nM) e/o bortezomib (2.5-20 nM); mentre
tecniche di Western blotting e microscopia a fluorescenza
sono state impiegate per identificare le proteine coinvolte nei
meccanismi molecolari responsabili dell’attività antiproliferativa
sinergica. I dati ottenuti dimostrano che, quando usate in
combinazione, dosi sub-tossiche o citostatiche di bortezomib
(2,5-5nM) e flavopiridolo (50-100 nM), inducono una marcata
inibizione della proliferazione cellulare in maniera direttamente
proporzionale alla durata di somministrazione (25-
50% a 24h; 70-90% 48h). In particolare, il nostro studio ha
dimostrato che la contemporanea somministrazione di flavopiridolo
e bortezomib è responsabile della condensazione e
frammentazione della cromatina della maggior parte delle cellule
di ALCL trattate, dell’accumulo di proteine coinvolte nell’arresto
del ciclo cellulare (p27, p21), e della degradazione di
proteine fondamentali per la sopravvivenza della cellula linfomatosa
(PARP), attraverso l’attivazione di meccanismi apoptotici
caspasi- dipendenti. In conclusione, questo studio dimostra
per la prima volta l’efficacia della concomitante somministrazione
di bortezomib e flavopiridolo nell’inibire la proliferazione
e indurre apoptosi in cellule di ALCL
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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