1,721,432 research outputs found
Zona C desde la casa
No full text¿Sabía usted que la fonoaudiología tiene también encuentros nacionales de discusión académica? La comunicación trasciende cualquier barrera desde la las limitaciones visuales hasta las de estructuras físicas por eso en Zona C al ritmo de rock alemán hablamos de la superación de barreras comunicativas y propuestas académicas nacientes muy innovadoras creadas por estudiantes de fonoaudiología UR además de datos que le ayudarán a conocer mejor que hacemos los fonoaudiólogos
Digital processing of the current noise evoked by kainate in cerebellar granule cells
No full textCurrent induced in cultured cerebellar granule cells by the bath application of kainate (500 microM) was measured using the conventional patch-clamp technique. Two different kinds of responses were observed after the agonist perfusion. Some cells exhibited small inward whole-cell currents: 116 +/- 40 pA (7 cells) at a clamp potential of -60 mV; in other cells, the agonist induced significantly larger currents: 420 +/- 35 pA (6 cells) at a clamp potential of -60 mV. The current flowing in the agonist-activated ionic channels was indirectly estimated by processing the fluctuations of whole-cell current by means of an original parametric method. Mean conductance of the underlying channels was then determined from the single-channel current estimated at different clamp potentials. In the cells exhibiting small inward currents, the mean conductance was equal to 0.5 +/- 0.2 pS (7 cells), whereas in the cells with large inward currents it was 3 +/- 0.4 pS (6 cells). This result gives a coherent explanation of the different kinds of responses observed at macroscopic level in the whole-cell current and confirms that kainate-activated channels can exhibit different levels of conductance
Calcium currents in cultured rat cortical neurons
No full textRat neocortical neurons grown in dissociated cell culture for 4-12 weeks were studied with whole-cell patch-clamp techniques in order to characterize the calcium currents present in these cells. When voltage-dependent Na and K currents were inhibited, depolarizations from negative holding potentials induced inward currents which had 3 components: a low threshold activated, small, relatively persistent component, which was completely inactivated at holding potentials more positive then -60 mV; a higher threshold, relatively persistent component (which was not inactivated at VH = -50 mV); and a higher threshold, larger, transient component. All 3 components were reduced by removal of Ca, and blocked by Cd and Ni at appropriate concentrations. The components were differentially affected by low concentrations of Ni (500 microM), nifedipine (500 microM) and Ba (1.8 mM). Only the first two components were present in very young neurons
Zona C. Il Kothon. Catalogo e tavole
No full textCatalogo della ceramica rinvenuta nella zona C, Mozia, campagna di scavi 200
Effects induced by the antiepileptic drug valproic acid upon the ionic currents recorded in rat neocortical neurons in cell culture
No full textRat neocortical neurons in culture were subjected to the whole cell mode of voltage clamping under experimental conditions designed to study Na+, Ca2+ and K+ currents in isolation. Following pharmacological blockade of most of the Ca2+ and K+ channels, depolarizing commands which brought the membrane potential from -80 to +10 mV elicited an inward current. This current was sensitive to tetrodotoxin (TTX) and was therefore caused by the opening of voltage-dependent channels permeable to Na+. Extracellular application of the antiepileptic drug valproic acid (VPA, 0.2-2mM) reduced in a dose-related, reversible way this Na+ current. VPA also evoked an increase of the voltage-dependent inward current recorded in the presence of TTX and thus presumably carried by Ca2+; this effect was seen in the presence of doses of VPA larger than 0.5 mM and was not reversible. Two types of outward K+ currents evoked by depolarizing steps in the presence of Na+ and Ca2+ channels blockers were not affected by VPA (up to 5 mM). Our data indicate that doses of VPA that are within the range present when it is used as an anticonvulsant, can influence inward currents generated by rat neocortical cells in culture. The reduction of the Na+, inward current is in line with findings obtained in mouse neurons by using standard intracellular recording techniques. This effect might represent an important mechanism of action for VPA in neocortex
Lamotrigine reduces voltage-gated sodium currents in rat central neurons in culture
No full textTo study the mechanism or mechanisms of action of lamotrigine (LTG) and, in particular, to establish its effects on the function of NA+ channels in mammalian central neurons
Calcium-activated potassium channels recorded from rat neocortical neurons in cell culture
No full textRat neocortical neurons in cell culture were studied with the patch-clamp technique in order to determine the properties of a large-conductance K+ channel in excised inside-out patches. In the presence of a physiological ionic gradient for K+ across the patch membrane ([K+]i = 120 mM; [K+]o = 3 mM), outward channel activity was detected when the patches were brought to membrane potential values less negative than -30 mV. Depolarization of the membrane increased the magnitude of the current. The I-V relationship displayed rectification at negative membrane potentials. When the I-V curve was differentiated the slope conductance calculated at 0 mV membrane potential was 120 pS. The single-channel permeability was 5.2 x 10(-13) cm/s and the current flow through the open K+ channel could be modeled using the constant-field electrodiffusion theory. K+ channel opening was not observed following removal of Ca2+ from the intracellular surface of the membrane. Our experiments indicate that, as in other cell types, rat neocortical neurons in culture exhibit a large-conductance K+ channel which is activated by Ca2+ acting on the cytoplasmic surface
Modulation of AMPA receptors in spinal motor neurons by the neuroprotective agent riluzole
No full textWe investigated the interaction of riluzole, a therapeutic agent used in amyotrophic lateral sclerosis (ALS), with alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor channels in mouse spinal motor neurons in culture using whole-cell patch-clamp recording techniques. Kainate elicited concentration-dependent (EC(50) = 35 microM) inward currents in all the patched cells. These responses were mediated primarily through the activation of AMPA receptors with a negligible contribution from kainate receptors, because bath application of 100 microM GYKI53655, a potent noncompetitive AMPA receptor antagonist, completely blocked the kainate-induced currents. Riluzole (0.5-100 microM) reduced in a dose-dependent manner the kainate-induced currents with an IC(50) of 1.54 microM in all tested neurons (n = 25) and this effect was found to be reversible. The response to kainate decreased in the presence of 1 microM riluzole in all spinal motor neurons tested, without changing its EC(50), indicating a noncompetitive mechanism of inhibition. The amplitude of the responses induced by kainate under control condition and during riluzole was a linear function of the membrane potential. The reversal potential of the current was not significantly different in the two experimental conditions, whereas the total conductance of the motor neurons for the currents induced by 100 microM kainate was reduced significantly in the presence of 1 microM riluzole (P < 0.05). These results reveal an interaction of riluzole with glutamatergic neurotransmission in spinal cord motor neurons and can contribute to explain its beneficial effect in the ALS treatment
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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