65 research outputs found
Detailed Facade Reconstruction for Mahattan-world Buildings
No full text3D building models play an important role in many real-world applications. Different models are suitable for different application scenarios based on their levels of detail. LOD3 models with facade details are crucial for many applications, such as virtual reality and urban simulation. Currently, 3D building models with lower LOD are largely available, but the number of LOD3 models is very limited. Most LOD3 reconstruction methods depend on manual operation, which is very time-consuming. How to automatically reconstruct the detailed facade for building models has remained a problem in computer vision. The problem can be seen as an image processing problem, but how to convert the 2D results into 3D smoothly should also be considered. In this project, we proposed a method to automatically reconstruct the detailed building models based on the Faster R-CNN. The method starts from a set of street view images, and the results are models with facade elements. A 3D point cloud can be extracted from the images using SfM and MVS, and the camera parameters can also be recovered. We take advantage of the high-quality facade images and parse the facades to detect their bounding boxes. The bounding boxes can match pretty well with the rectangular shape of the facade elements. The 2D facade elements can be added to the 3D building model based on the camera parameters. The process is very efficient and automatic. The regularity of the facade elements will be reserved, making the result more convincing. Our method includes four main steps: (1) coarse model reconstruction, (2) facade image selection and rectification, (3) facade element detection and regularization, and (4) detailed facade reconstruction. Experiment results show that our method can produce reliable building models with facade details for many different situations. It can work for both the multi-face building blocks and the street side buildings. Our test shows that the window detection performance is pretty good. The object detection is extremely fast, and the whole pipeline is lightweight and efficient. In theory, the method can also be extended to reconstruct large-scale city models, which means it has broad application prospects.Geomatic
Thymopentin improves the survival of septic mice by promoting the production of 15-deoxy-prostaglandin J2 and activating the PPARγ signaling pathway
No full textSepsis, a systemic inflammatory response syndrome (SIRS) caused by infection, is a major public health concern with limited therapeutic options. Infection disturbs the homeostasis of host, resulting in excessive inflammation and immune suppression. This has prompted the clinical use of immunomodulators to balance host response as an alternative therapeutic strategy. Here, we report that Thymopentin (TP5), a synthetic immunomodulator pentapeptide (Arg-Lys-Asp-Val-Tyr) with an excellent safety profile in the clinic, protects mice against cecal ligation and puncture (CLP)-induced sepsis, as shown by improved survival rate, decreased level of pro-inflammatory cytokines and reduced ratios of macrophages and neutrophils in spleen and peritoneum. Regarding mechanism, TP5 changed the characteristics of LPS-stimulated macrophages by increasing the production of 15-deoxy-Δ12,14-prostaglandin J2 (15-d-PGJ2). In addition, the improved effect of TP5 on survival rates was abolished by the peroxisome proliferator-activated receptor γ (PPARγ) antagonist GW9662. Our results uncover the mechanism of the TP5 protective effects on CLP-induced sepsis and shed light on the development of TP5 as a therapeutic strategy for lethal systemic inflammatory disorders.Fil: Zhang, Ye. China Pharmaceutical University; ChinaFil: Yang, Xue. China Pharmaceutical University; ChinaFil: Yan, Wenchao. China Pharmaceutical University; ChinaFil: Li, Rui. China Pharmaceutical University; ChinaFil: Ye, Qian. China Pharmaceutical University; ChinaFil: You, Linjun. China Pharmaceutical University; ChinaFil: Xie, Wenhao. China Pharmaceutical University; ChinaFil: Mo, Kun. China Pharmaceutical University; ChinaFil: Fu, Ruifeng. China Pharmaceutical University; ChinaFil: Wang, Yanxiang. China Pharmaceutical University; ChinaFil: Chen, Yufei. China Pharmaceutical University; ChinaFil: Hou, Hui. China Pharmaceutical University; ChinaFil: Yang, Yong. China Pharmaceutical University; ChinaFil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; ArgentinaFil: Di, Qin. Nanjing Sport Institute; ChinaFil: Li, Xianjing. China Pharmaceutical University; Chin
TRPC absence induces pro-inflammatory macrophages and gut microbe disorder, sensitizing mice to colitis
No full textThe transient receptor potential canonical (TRPC) channels, encoded in seven non-allelic genes, are important contributors to calcium fluxes, are strongly associated with various diseases. Here we explored the consequences of ablating all seven TRPCs in mice focusing on colitis. We discovered that absence of all seven TRPC proteins in mice (TRPC HeptaKO mice) promotes the development of dextran sulfate sodium (DSS)-induced colitis. RNA-sequence analysis highlighted an extremely pro-inflammatory profile in colons of DSS-treated TRPC HeptaKO mice, with an amount of increased pro-inflammatory cytokines and chemokines. Flow cytometry analysis showed that the infiltration of Ly6Chi monocytes and neutrophils in colonic lamina propria was significantly increased in DSS-treated TRPC HeptaKO mice. Results also revealed that macrophages from TRPC HeptaKO mice exhibited M1 polarization and enhanced secretion of pro-inflammatory factors. In addition, the composition of gut microbiota was markedly disturbed in DSS-treated TRPC HeptaKO mice. However, upon antibiotic cocktail (Abx)-treatment, TRPC HeptaKO mice showed no significant differences with WT mice in disease severity. Collectively, these data suggest that ablation of all TRPCs promotes the development of DSS-induced colitis by inducing pro-inflammatory macrophages and gut microbiota disorder.Fil: Lin, Yanting. State Key Laboratory Of Natural Medicines; ChinaFil: Cui, Xinmeng. State Key Laboratory Of Natural Medicines; ChinaFil: Cao, Qiuhua. State Key Laboratory Of Natural Medicines; ChinaFil: Bi, Ran. State Key Laboratory Of Natural Medicines; ChinaFil: Liu, Yiming. State Key Laboratory Of Natural Medicines; ChinaFil: Jing, Dongquan. State Key Laboratory Of Natural Medicines; ChinaFil: Yue, Chongxiu. State Key Laboratory Of Natural Medicines; ChinaFil: Zhao, Qixiang. State Key Laboratory Of Natural Medicines; ChinaFil: Wang, Yue. State Key Laboratory Of Natural Medicines; ChinaFil: Liu, Siliang. State Key Laboratory Of Natural Medicines; ChinaFil: Su, Yali. State Key Laboratory Of Natural Medicines; ChinaFil: Formoso, Karina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Susperreguy, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; ArgentinaFil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. National Institute Of Environmental Health Sciences; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Freichel, Marc. Ruprecht Karls Universitat Heidelberg; AlemaniaFil: Yang, Yong. State Key Laboratory Of Natural Medicines; ChinaFil: You, Linjun. State Key Laboratory Of Natural Medicines; ChinaFil: Gao, Xinghua. State Key Laboratory Of Natural Medicines; Chin
Electrophysiological evidence of categorical perception of Chinese lexical tones in attentive condition
No full textPrevious electrophysiological evidence supports categorical perception of Chinese lexical tones at the preattentive stage (Xi and colleagues). In this study, we examined participants' attentive responses to tonal continua in an event-related potential experiment that recorded their N2b and P3b oddball responses. We found that for both the N2b and the P3b component, the responses elicited by the within-category deviants were similar in the left and the right recording sites. However, the across-category deviants elicited larger responses in the left recording sites than in the right sites, reflecting conscious phonological processing of lexical tones. These results provide electrophysiological correlates of categorical perception of Chinese lexical tones in later stages associated with controlled processes. NeuroReport 23: 35-39 (C) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins
Cortical Dynamics of Acoustic and Phonological Processing in Speech Perception
No full textIn speech perception, a functional hierarchy has been proposed by recent functional neuroimaging studies: Core auditory areas on the dorsal plane of superior temporal gyrus (STG) are sensitive to basic acoustic characteristics, whereas downstream regions, specifically the left superior temporal sulcus (STS) and middle temporal gyrus (MTG) ventral to Heschl's gyrus (HG) are responsive to abstract phonological features. What is unclear so far is the relationship between the dorsal and ventral processes, especially with regard to whether low-level acoustic processing is modulated by high-level phonological processing. To address the issue, we assessed sensitivity of core auditory and downstream regions to acoustic and phonological variations by using within-and across-category lexical tonal continua with equal physical intervals. We found that relative to within-category variation, across-category variation elicited stronger activation in the left middle MTG (mMTG), apparently reflecting the abstract phonological representations. At the same time, activation in the core auditory region decreased, resulting from the top-down influences of phonological processing. These results support a hierarchical organization of the ventral acoustic-phonological processing stream, which originates in the right HG/STG and projects to the left mMTG. Furthermore, our study provides direct evidence that low-level acoustic analysis is modulated by high-level phonological representations, revealing the cortical dynamics of acoustic and phonological processing in speech perception. Our findings confirm the existence of reciprocal progression projections in the auditory pathways and the roles of both feed-forward and feedback mechanisms in speech perception
Galphai1 and Galphai3 regulate macrophage polarization by forming a complex containing CD14 and Gab1
No full textHeterotrimeric G proteins have been implicated in Toll-like receptor 4 (TLR4) signaling in macrophages and endothelial cells. However, whether guanine nucleotide-binding protein G(i) subunit alpha-1 and alpha-3 (Gαi1/3) are required for LPS responses remains unclear, and if so, the underlying mechanisms need to be studied. In this study, we demonstrated that, in response to LPS, Gαi1/3 form complexes containing the pattern recognition receptor (PRR) CD14 and growth factor receptor binding 2 (Grb2)-associated binding protein (Gab1), which are required for activation of PI3K-Akt signaling. Gαi1/3 deficiency decreased LPS-induced TLR4 endocytosis, which was associated with decreased phosphorylation of IFN regulatory factor 3 (IRF3). Gαi1/3 knockdown in bone marrow-derived macrophage cells (Gαi1/3 KD BMDMs) exhibited an M2-like phenotype with significantly suppressed production of TNF-α, IL-6, IL-12, and NO in response to LPS. The altered polarization coincidedwith decreased Akt activation. Further, Gαi1/3 deficiency caused LPS tolerance in mice. In vitro studies revealed that, in LPS-tolerant macrophages, Gαi1/3 were down-regulated partially by the proteasome pathway. Collectively, the present findings demonstrated that Gαi1/3 can interact with CD14/Gab1, which modulates macrophage polarization in vitro and in vivo.Fil: Li, Xiaolin. China Pharmaceutical University. Center for New Drug Safety Evaluation and Research; ChinaFil: Wang, Duowei. China Pharmaceutical University. Center for New Drug Safety Evaluation and Research; ChinaFil: Chen, Zen. China Pharmaceutical University. Center for New Drug Safety Evaluation and Research; ChinaFil: Lu, Ermei. China Pharmaceutical University. Center for New Drug Safety Evaluation and Research; ChinaFil: Wang, Zhuo. China Pharmaceutical University. Center for New Drug Safety Evaluation and Research; ChinaFil: Duan, Jingjing. China Pharmaceutical University. Center for New Drug Safety Evaluation and Research; ChinaFil: Tian, Wei. China Pharmaceutical University. Center for New Drug Safety Evaluation and Research; ChinaFil: Wang, Yun. China Pharmaceutical University. Center for New Drug Safety Evaluation and Research; ChinaFil: You, Linjun. China Pharmaceutical University. Center for New Drug Safety Evaluation and Research; ChinaFil: Zou, Yulian. China Pharmaceutical University. Center for New Drug Safety Evaluation and Research; ChinaFil: Cheng, Yan. China Pharmaceutical University. Center for New Drug Safety Evaluation and Research; ChinaFil: Zhu, Qingyi. Jiangsu Province Hospital of Traditional Chinese Medicine. Departament of Urology; ChinaFil: Wan, Xiaojian. Second Military Medical University. Department of Anesthesiology and Intensive Care Medicine, Changhai Hospita; ChinaFil: Xia, Tao. China Pharmaceutical University. Center for New Drug Safety Evaluation and Research; ChinaFil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. National Institute of Environmental Health Sciences. Laboratory of Neurobiology, ; Estados UnidosFil: Yang, Yong. China Pharmaceutical University. Center for New Drug Safety Evaluation and Research; Chin
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A framework for natural disaster memorial park design 5/12 Earthquake Memorial Park design
No full textAn earthquake with a magnitude of 8.0 on the Richter scale rocked Sichuan on May 12, 2008, eventually claiming 69,227 lives, and 374,643 injured. The quake toppled houses and schools, virtually destroying entire villages, and irreparably changed everyone's world there. The quake severely affected an estimated 45,547,565 residents in Sichuan province, damaged approximately 4,300,000 structures, and destroyed buildings including homes, businesses, schools, and hospitals. Thousands of innocent people lost their precious lives in this disaster. Among these people, there were young students reading out beautiful poems in books; there were mamas preparing hot milk for their kids in the morning; there were...however, those bright lives, at that moment, all became cold numbers in the history books. A natural disaster memorial park is needed to memorialize all the survivors and victims of this great disaster, to tell stories of survival and loss, to encourage people surmounting the tragedy bravely, and to provide a refuge for future disasters. Throughout the research conducted after the May 12 earthquake, the author has observed that no integrated system of planning and design has been established in natural disaster memorial park design. The thesis defines the conception of a memorial landscape and natural disaster memorial park, reviews the history of natural disaster memorial parks, and sorts through many cases of memorial parks. A general design guideline and theory for natural disaster memorial park design will be summarized from case studies, and this guideline will be interpreted particularly in terms of the 5/12 Earthquake Memorial Park design in China. The result of this research is an extension of an overall knowledge base and a theory explaining the difference between "natural disaster memorials" and "war memorials" design and summarizing the basic elements that constitute the physical and psychological characteristics of natural disaster memorial park design. The 5/12 Earthquake Memorial design will be a new application of the integration of the new theory about Natural Disaster Memorial Landscape design and will provide guidance and reference for park planners and designers to promote multiple functions in memorial park design in the future
J Orthop Res
No full textVariations in chondrocyte density and organization in cartilage histology sections are associated with osteoarthritis progression. Rapid, accurate quantification of these two features can facilitate the evaluation of cartilage health and advance the understanding of their significance. The goal of this work was to adapt deep-learning-based methods to detect articular chondrocytes and chondrocyte clones from safranin-O-stained cartilage to evaluate chondrocyte cellularity and organization. The U-net and "you-only-look-once" (YOLO) models were trained and validated for identifying chondrocytes and chondrocyte clones, respectively. Validated models were then used to quantify chondrocyte and clone density in talar cartilage from Yucatan minipigs sacrificed 1 week, 3, 6, and 12 months after fixation of an intra-articular fracture of the hock joint. There was excellent/good agreement between expert researchers and the developed models in identifying chondrocytes/clones (U-net: R| \u2009=\u20090.93, y\u2009=\u20090.90x-0.69; median F1 score: 0.87/YOLO: R| \u2009=\u20090.79, y\u2009=\u20090.95x; median F1 score: 0.67). Average chondrocyte density increased 1 week after fracture (from 774 to 856 cells/mm| ), decreased substantially 3 months after fracture (610 cells/mm| ), and slowly increased 6 and 12 months after fracture (638 and 683 cells/mm| , respectively). Average detected clone density 3, 6, and 12 months after fracture (11, 11, 9 clones/mm| ) was higher than the 4-5 clones/mm| detected in normal tissue or 1 week after fracture and show local increases in clone density that varied across the joint surface with time. The accurate evaluation of cartilage cellularity and organization provided by this deep learning approach will increase objectivity of cartilage injury and regeneration assessments.P50 AR055533/AR/NIAMS NIH HHSUnited States/R49 CCR721745/CC/CDC HHSUnited States/P50 AR055533/AR/NIAMS NIH HHSUnited States
3D Representations for Visual Insight
No full textAs a method that can accurately represent 3D spatial information, point cloud visualisation for indoor environments is still a relatively unexplored field of research. Our client for this project, the Dutch National Police, requested a variety of potential solutions for visualising (unfamiliar) indoor environments that can be viewed by both external command centres, and internal operations units. Currently, unknown interior layouts (or layouts that are different in practise to what is stated on paper) can have serious, sometimes even life-threatening, consequences in time-sensitive situations. This project uses a game engine to directly visualise point cloud data input of indoor environments. The primary aim is to find ways of clearly communicating a point cloud of an environment to a layman viewer through intuitive visualisations, to aid decision-making in high-stress moments. The final product is a variety of visualisation concepts, hosted within a game engine in order to allow users to navigate throughout (part of) a building, and customise certain interaction features. To aid the layman viewer, various interpretation methods (e.g. cartography) are considered. The Unreal Engine 4 (UE4) project was designed and developed based on the requirements given by Dutch Police, and consisted of 4 modules: data preprocessing, render style, functional module, and User Interface (UI). An indoor point cloud dataset is used for the implementation, while corresponding mesh and voxel models are also respectively generated and evaluated as reference objects. The implemented software product is evaluated based on a Structured Expert Evaluation Method and finally our project result demonstrates that point cloud has unique advantages for visualisation of indoor environments especially in pre-processing efficiency, detail level, and volume perception.https://github.com/peterliu502/IndoorPointCloudViewer Repository link The GitHub repository of this project.Synthesis Project 2021Geomatic
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